Artemisiae argyi(A.argyi)is a traditional medical plant,with medicinal and economic value.It has been used to treat diseases for more than 2000 years,and its application has been expanded in recent years with the in-d...Artemisiae argyi(A.argyi)is a traditional medical plant,with medicinal and economic value.It has been used to treat diseases for more than 2000 years,and its application has been expanded in recent years with the in-depth research on the chemical composition and pharmacology.It is mainly used clinically for the treatment of“cold pain in the abdomen,cold clotting in the menstrual channels,cold infertility in the uterus,fetal movement,vomiting blood,epistaxis,pus and blood dysentery,and external treatment of itchy skin”and other symptoms.This paper reviews the research on the biological activity and chemical composition of A.argyi by scholars in recent decades.展开更多
Individuals with Glucose-6-phosphate dehydrogenase (G6PD) deficiency are susceptible to hemolytic anemia when exposed to pro-oxidant substances. This study investigates the hemolytic impact of Artemisia annua (A. annu...Individuals with Glucose-6-phosphate dehydrogenase (G6PD) deficiency are susceptible to hemolytic anemia when exposed to pro-oxidant substances. This study investigates the hemolytic impact of Artemisia annua (A. annua) extracts in G6PD-deficient subjects through a mixed experimental approach. In the in vitro phase, red blood cells from G6PD-deficient individuals and rats induced with Dehydroepiandrosterone (DHEA) were exposed to various concentrations of A. annua infusion, with distilled water and physiological saline as positive and negative controls respectively. The in vivo study involved G6PD-deficient Wistar rats divided into three groups receiving A. annua infusion, quinine (positive control), and distilled water (negative control) via gavage. Blood samples were collected for biochemical and hematological analyses. Notably, at a 40% concentration of A. annua infusion, there was a significant increase in the hemolysis rate of G6PD-deficient red blood cells compared to controls (p A. annua exhibited elevated aspartate aminotransferase (129.25 ± 4.55 U/L vs. 80.09 ± 4.03 U/L;p A. annua infusion tested positive for saponins. These findings underscore the risk of hemolysis in G6PD-deficient individuals upon ingesting A. annua.展开更多
Background:Artemisia vulgaris,a medicinal aromatic plant,is widely used as a food item,tonic pharmaceutical,and cosmetic industry additive owing to its antibacterial,antihypertensive,hepatoprotective,antioxidant,and a...Background:Artemisia vulgaris,a medicinal aromatic plant,is widely used as a food item,tonic pharmaceutical,and cosmetic industry additive owing to its antibacterial,antihypertensive,hepatoprotective,antioxidant,and antispasmodic properties.But the effect of different geographic locations on the chemical composition and bioactivities of its extracts is unclear.Methods:Biological activities of essential oils and ethanol extracts of three varieties of Artemisia vulgaris leaves,which are grown in Shanxi province China,were studied.Results:Gas chromatography-mass spectrometry analysis revealed that the main components of essential oils were terpenes and ketones.Essential oils and ethanol extract of Artemisia vulgaris leaves possessed good antioxidant activities,and their half maximal inhibitory concentrations determined using 1,1-diphenyl-2-picrylhydrazyl and 2,2’-azinobis-(3-ethylbenzthiazoline-6-sulphonate)assays were 57.0 and 22.9μg/mL,respectively.The essential oils also exhibited remarkable antibacterial activity against three foodborne pathogenic bacterial strains.The ethanol extract presented a high anticancer activity against the MGC-803 human gastric cancer cell line.Conclusion:These biological activities were well correlated with the composition of the extract and EOs,which in turn is affected by the genetic composition of Artemisia vulgaris and geographic location and diverse climatic condition under which it is grown.These findings demonstrate the remarkable potential of Artemisia vulgaris as a valuable source of antioxidant,antibacterial,and anticancer agents.展开更多
All nuclei in mesophyll cells of Artemisia marschalliana are located in vacuoles and occupy up to 90% of their volume. The ultrastructural organization of chromatin in nuclei shows different degrees of its decondensat...All nuclei in mesophyll cells of Artemisia marschalliana are located in vacuoles and occupy up to 90% of their volume. The ultrastructural organization of chromatin in nuclei shows different degrees of its decondensation, up to complete separation of DNA from histones. It is possible that the separation of DNA from histones enables Artemisia to grow in soils with high salinity.展开更多
[Objectives]This paper was to figure out whether the dominant bacterial community has the role and effect of bacterial community and its defense mechanism against potential pathogenic fungi in Artemisia annua,and thus...[Objectives]This paper was to figure out whether the dominant bacterial community has the role and effect of bacterial community and its defense mechanism against potential pathogenic fungi in Artemisia annua,and thus establish a systematic model of bacteria-fungus-plant.[Methods]Fifty-eight strains of bacteria and one strain of pathogenic fungi,Globisporangium ultimatum,were used for the experiments.These 58 bacterial strains were assembled into a bacterial community,and the bacteria with abundance in the top 1%were reassembled into a dominant bacterial community as measured by 16S rDNA.[Results]The growth of A.annua seedlings inoculated with bacterial communities and pathogenic fungi or dominant bacterial communities and pathogenic fungi was significantly better than that of A.annua seedlings inoculated with pathogenic fungi during in vitro confrontation,which was evident in both enzymatic and non-enzymatic antioxidant assays.[Conclusions]The results suggest that the dominant bacterial community has a crucial role as a representative core microbial community of synthetic bacterial community,which can protect plants by interfering with the growth of phytopathogenic fungi mediated by chemical signals,and can be used as the main synthetic community of biocides to achieve the effect of biocontrol.展开更多
Objective: To identify the chemical constituents and biological activities of essential oil and crude methanol extract of Artemisia vulgaris(A.vulgaris) and Gaultheria fragrantissima(G.fragrantissima).Methods: Phytoch...Objective: To identify the chemical constituents and biological activities of essential oil and crude methanol extract of Artemisia vulgaris(A.vulgaris) and Gaultheria fragrantissima(G.fragrantissima).Methods: Phytochemical screening, total phenolic and flavonoid content, antibacterial activities, anti-oxidant assay of the crude extract were carried out to identify the biological activities and phytonutrients present in the extract.Furthermore, the chemical constituents present in the essential oil and crude methanol extract were analyzed using gas chromatography mass spectroscopy and high performance liquid chromatography(HPLC) analysis.Results: Gas chromatography mass spectroscopy analysis of essential oil from the aerial part of A.vulgaris revealed 24 different compounds in it.Sabinene(11.29%), b-thujone(19.19%), chrysanthenone(4.48%), camphor(11.89%), borneol(4.44%) and germacrene D(8.42%) were the major compounds.Similarly, leaves of G.fragrantissima contained methyl salicylate(95%) and asarone(4.64%).Furthermore, methanol extract of leaves of A.vulgaris and G.fragrantissima were found rich in the total flavonoids and phenolic content.HPLC analysis of the methanol extract of leaves A.vulgaris revealed the presence of morin and luteolin, whereas rutin was found as a major flavonoids compound in the leaves of G.fragrantissima.Further, methanol extract of the A.vulgaris and G.fragrantissima showed the highest antioxidant and antibacterial properties compared to the essential oil.Conclusions: The HPLC analysis of the methanol extract of A.vulgaris shows the presence of luteolin and morin, whereas G.fragrantissima reveals the presence of rutin and a glycosylated flavonoids.Results reveal that A.vulgaris oil is the rich source of monoterpene and sesquiterpene compounds.Furthermore, A.vulgaris and G.fragrantissima are the rich source of the phenolic and flavonoids compounds and show good antioxidant and antibacterial activity.展开更多
[Objectives] The aim was to study the chemical composition of the aboveground part of Artemisia subdigitata Mattf. [Methods]Using the systemic preparative test,the water extract,ethanol extract and petroleum ether ext...[Objectives] The aim was to study the chemical composition of the aboveground part of Artemisia subdigitata Mattf. [Methods]Using the systemic preparative test,the water extract,ethanol extract and petroleum ether extract of the aboveground part of A. subdigitata were subjected to physiochemical identification,and the contained chemical constituents were identified preliminarily. [Results] The preliminary comparisons of preparative test results of the aboveground part of A. subdigitata suggested that the whole plant may contain saponins,tannins,phenols,flavonoids,anthraquinones,coumarins,lactones,alkaloids,sterides or triterpenes,volatile oils and oils. [Conclusions]A. subdigitata contains a variety of chemical constituents. This study provides certain experimental basis for the further research and development and utilization of A. subdigitata.展开更多
Artemisia vulgaris L. was collected from various regions in the USA and Montreal Canada. Gas Chromatography-Mass Spectrometry was used to identify the analytes present in the volatiles extracted by headspace solid-pha...Artemisia vulgaris L. was collected from various regions in the USA and Montreal Canada. Gas Chromatography-Mass Spectrometry was used to identify the analytes present in the volatiles extracted by headspace solid-phase microextraction of the crushed leaves and flowers. Four distinct chemotypes are were found: One featuring the coexistence of ar-curcumene and α-zingiberene;two marked by the presence or absence of thujone and santolinatriene;and a fourth characterized by the presence of crysanthenyl acetate (40%). DNA was used to confirm the identity of Artemisia vulgaris L.展开更多
Background: Artemisia afra is an indigenous plant used in Africa. It is used in traditional medicine to treat a variety of diseases caused by bacterial infections. As a result, developing innovative therapeutic method...Background: Artemisia afra is an indigenous plant used in Africa. It is used in traditional medicine to treat a variety of diseases caused by bacterial infections. As a result, developing innovative therapeutic methods using natural ingredients to combat pathogenic germs is critical. Methodology: Artemisia afra leaves were extracted using maceration extraction utilizing three solvents (petroleum ether, ethanol, and dichloromethane). Fresh leaves were extracted using hydrodistillation. The agar well diffusion method was used to test the crude extract and essential oil leaves of Artemisia afra against different human pathogenic bacteria strains (E. coli, S. aureus, E. faecalis, and K. pneumonia) at different concentrations in the presence of a positive (ciprofloxacin 5.0 μg) and negative (DMSO) control. Results: Artemisia afra revealed the presence of alkaloids, flavonoids, tannins, saponins, anthraquinones, terpenoids, coumarins, phenolic quinones, cardiac glycosides, and steroids. It showed that the highest antibacterial activity given by the ethanol extract had highly inhibition zones against gram-positive and gram-negative bacteria. The essential oil extract was effective against all tested bacteria. Conclusion: Plant crude extracts and essential oils may have antibacterial properties due to the synergistic activity of two or more active secondary metabolites.展开更多
Background:As one of the most popular traditional Chinese medicines(TCMs)for the treatment of various liver diseases,virgate wormwood herb(Artemisia capillaris Thunb.)has a long application history in TCM practices.It...Background:As one of the most popular traditional Chinese medicines(TCMs)for the treatment of various liver diseases,virgate wormwood herb(Artemisia capillaris Thunb.)has a long application history in TCM practices.It has been well established that the chemical composition is responsible for the pronounced therapeutic spectrum of A.capillaris.Although they are comprehensive,the time-intensive liquid chromatography coupled to tandem mass spectrometry(LCeMS/MS)assays cannot fully satisfy the analytical measurement workload from many test samples.Direct infusion-MS/MS(DIeMS/MS)may be the optimal choice to achieve high-throughput analysis if the mass spectrometer can universally record MS2 spectra.Methods:According to the application of gas phase ion fractionation concept,the MS/MSALL program enables to gain MS2 spectrum for each nominal m/z value with a data-independent acquisition algorithm via segmenting the entire MS1 ion cohort into sequential ion pieces with 1 Da width,when sufficient measurement time is allowed by DI approach.Here,rapid clarification of the chemical composition was attempted for A.capillaris using DIeMS/MSALL.A.capillaris extract was imported directly into the electrospray ionization interface to obtain the MS/MSALL measurement.After the MS1-MS2 dataset was well organized,we focused on structural characterization through retrieving information from the available databases and literature.Results:Twenty-six compounds were found,including 12 caffeoyl quinic acid derivatives,7 flavonoids,and 7 compounds belonging to other chemical families.Among them,24 ones were structurally identified.Compared with the LCeMS/MS technique,DIeMS/MSALL has the advantages of low-costing,solvent-saving,and time-saving.Conclusions:Chemical profiling of A.capillaris extract was accomplished within 5 min by DIeMS/MSALL,and this technique can be an alternative choice for chemical profile characterization of TCMs due to its extraordinary high-throughput advantage.展开更多
The aim of this study was to investigate the chemical composition of the essential oils of “Covid-Organics” of Madagascar (62% Artemisia annua and two other undisclosed medicinal plants) used as curative and prevent...The aim of this study was to investigate the chemical composition of the essential oils of “Covid-Organics” of Madagascar (62% Artemisia annua and two other undisclosed medicinal plants) used as curative and preventive treatments of Covid-19, to identify its constituent species. The essential oils isolated by hydro-distillation from two samples (curative and preventive) were analyzed by GC-FID and GC-MS. These essential oils (curative and preventive) were mainly dominated by camphor (17.9% and 11.9%, respectively), spathulenol (4.8% and 11.8%, respectively), α-acorenol (4.3% and 3.7%, respectively), (E)-β-caryophyllene (3.4% and 4.2%, respectively), 1,8-cineole (3.1% and 3.6%, respectively), hexadecanoïc acid (3.8% and 3.2%, respectively) and caryophyllene oxide (3.4% and 2.4%, respectively). From the chemical composition, two species were identified, A. annua characterised by camphor and Cinnamomumcamphora (Ravintsara) characterised by 1,8-cineole and sabinene. However, we were unable to identify the third species.展开更多
Artemisia argyi is a traditional Chinese herb,which is widely distributed in various areas in China.In recent years,due to the development of A.argyi related industries and the continuous improvement of people’s heal...Artemisia argyi is a traditional Chinese herb,which is widely distributed in various areas in China.In recent years,due to the development of A.argyi related industries and the continuous improvement of people’s health awareness,experts and scholars are concerned about the nutrients and effects of A.argyi.A.argyi is rich in carbohydrates,proteins,essential amino acids,fatty acids,minerals,vitamins,dietary fibers,flavonoids and other nutrients.It has the effects of antioxidant,anti-tumor,antibacterial,antiinflammatory,neuroprotection and immune regulations,and has been widely applied in the fields of COVID-19 prevention,food health care,food preservation and feed breeding,and has good development potential.This review summarized the research and application of A.argyi nutrients,in order to provide reference and application for further study.展开更多
Malaria causes many deaths around the world, particularly in Africa, which ultimately affects the socio-economic development of African countries. The resistance of Plasmodium falciparum to quinine-based drugs led to ...Malaria causes many deaths around the world, particularly in Africa, which ultimately affects the socio-economic development of African countries. The resistance of Plasmodium falciparum to quinine-based drugs led to new studies showing the efficiency of new artemisin-based drugs. The molecule artemisin is extracted from Artemisia annua a plant from China that has been used for decades in traditional Chinese medicine. The purpose of this study is to improve the production of sweet wormwood (Artemisia annua) using organic fertilizers in the north of Cote d’Ivoire. To do so, a morpho-pedological characterization of the study site was firstly performed to determine the soil type and their fertility level. Then, a randomized complete block system including two factors (the quantity of compost and the plant density) was implemented to test the effect of organic amendment and plant arrangement on the growth of Artemisia annua. Six treatments were set up: a control plot (no compost) where the plants are arranged in square (T0D1) and the plants are arranged in staggered (T0D2). Then, a treatment with compost addition of 25 t/ha where the plants are arranged in square (T1D1) and in staggered (T1D2). A treatment with compost addition of 50 t/ha where plants are arranged in square (T2D1) and in staggered (T2D2). Our results showed that the soils hosting our experimentation are Arenithic Plinthic Ferrasols with a very low level of fertility, prone to leaching and erosion. T1D2 and T2D2 treatments obtained the highest yields of 2.82 t/ha and 3.91 t/ha, respectively. Our findings indicate that a high dose of organic amendment combined with a staggered plant arrangement strongly improves the biomass production of sweet wormwood. This is in agreement with previous studies showing that the addition of organic matter can restore the level of soil fertility by increasing soil porosity and the activity of micro and macroorganisms.展开更多
基金This work was financially supported by National Nature Science Foundation of China(81973284)Scientific Research Foundation of the Education Department of Liaoning Province(LJKZ0944).
文摘Artemisiae argyi(A.argyi)is a traditional medical plant,with medicinal and economic value.It has been used to treat diseases for more than 2000 years,and its application has been expanded in recent years with the in-depth research on the chemical composition and pharmacology.It is mainly used clinically for the treatment of“cold pain in the abdomen,cold clotting in the menstrual channels,cold infertility in the uterus,fetal movement,vomiting blood,epistaxis,pus and blood dysentery,and external treatment of itchy skin”and other symptoms.This paper reviews the research on the biological activity and chemical composition of A.argyi by scholars in recent decades.
文摘Individuals with Glucose-6-phosphate dehydrogenase (G6PD) deficiency are susceptible to hemolytic anemia when exposed to pro-oxidant substances. This study investigates the hemolytic impact of Artemisia annua (A. annua) extracts in G6PD-deficient subjects through a mixed experimental approach. In the in vitro phase, red blood cells from G6PD-deficient individuals and rats induced with Dehydroepiandrosterone (DHEA) were exposed to various concentrations of A. annua infusion, with distilled water and physiological saline as positive and negative controls respectively. The in vivo study involved G6PD-deficient Wistar rats divided into three groups receiving A. annua infusion, quinine (positive control), and distilled water (negative control) via gavage. Blood samples were collected for biochemical and hematological analyses. Notably, at a 40% concentration of A. annua infusion, there was a significant increase in the hemolysis rate of G6PD-deficient red blood cells compared to controls (p A. annua exhibited elevated aspartate aminotransferase (129.25 ± 4.55 U/L vs. 80.09 ± 4.03 U/L;p A. annua infusion tested positive for saponins. These findings underscore the risk of hemolysis in G6PD-deficient individuals upon ingesting A. annua.
基金This work was financially supported by the National Natural Science Foundation of China(Grant No.32001817)the Science and Technology Innovation Plan of Colleges and Universities of Shanxi Province(2020L0298)the College student innovation project of North University of China and the start-up funds for scientific research at North University of China(No.304-1101285714).
文摘Background:Artemisia vulgaris,a medicinal aromatic plant,is widely used as a food item,tonic pharmaceutical,and cosmetic industry additive owing to its antibacterial,antihypertensive,hepatoprotective,antioxidant,and antispasmodic properties.But the effect of different geographic locations on the chemical composition and bioactivities of its extracts is unclear.Methods:Biological activities of essential oils and ethanol extracts of three varieties of Artemisia vulgaris leaves,which are grown in Shanxi province China,were studied.Results:Gas chromatography-mass spectrometry analysis revealed that the main components of essential oils were terpenes and ketones.Essential oils and ethanol extract of Artemisia vulgaris leaves possessed good antioxidant activities,and their half maximal inhibitory concentrations determined using 1,1-diphenyl-2-picrylhydrazyl and 2,2’-azinobis-(3-ethylbenzthiazoline-6-sulphonate)assays were 57.0 and 22.9μg/mL,respectively.The essential oils also exhibited remarkable antibacterial activity against three foodborne pathogenic bacterial strains.The ethanol extract presented a high anticancer activity against the MGC-803 human gastric cancer cell line.Conclusion:These biological activities were well correlated with the composition of the extract and EOs,which in turn is affected by the genetic composition of Artemisia vulgaris and geographic location and diverse climatic condition under which it is grown.These findings demonstrate the remarkable potential of Artemisia vulgaris as a valuable source of antioxidant,antibacterial,and anticancer agents.
文摘All nuclei in mesophyll cells of Artemisia marschalliana are located in vacuoles and occupy up to 90% of their volume. The ultrastructural organization of chromatin in nuclei shows different degrees of its decondensation, up to complete separation of DNA from histones. It is possible that the separation of DNA from histones enables Artemisia to grow in soils with high salinity.
基金Supported by Science and Technology Plan Project of Guizhou Province,China(QKH JC[2020]1Y179)Key Field Project of Education Department of Guizhou Province(QJHKYZ[2021]044)+1 种基金Forestry Research Project of Guizhou Province(QLKH[2021]11)Project of Guizhou Provincial Characteristic Key Laboratory(QJHKY[2021]002).
文摘[Objectives]This paper was to figure out whether the dominant bacterial community has the role and effect of bacterial community and its defense mechanism against potential pathogenic fungi in Artemisia annua,and thus establish a systematic model of bacteria-fungus-plant.[Methods]Fifty-eight strains of bacteria and one strain of pathogenic fungi,Globisporangium ultimatum,were used for the experiments.These 58 bacterial strains were assembled into a bacterial community,and the bacteria with abundance in the top 1%were reassembled into a dominant bacterial community as measured by 16S rDNA.[Results]The growth of A.annua seedlings inoculated with bacterial communities and pathogenic fungi or dominant bacterial communities and pathogenic fungi was significantly better than that of A.annua seedlings inoculated with pathogenic fungi during in vitro confrontation,which was evident in both enzymatic and non-enzymatic antioxidant assays.[Conclusions]The results suggest that the dominant bacterial community has a crucial role as a representative core microbial community of synthetic bacterial community,which can protect plants by interfering with the growth of phytopathogenic fungi mediated by chemical signals,and can be used as the main synthetic community of biocides to achieve the effect of biocontrol.
基金supported by Academy Partnership Program Track 1-1 funded by KOICA(APP-Track 1-1 2015-0249)
文摘Objective: To identify the chemical constituents and biological activities of essential oil and crude methanol extract of Artemisia vulgaris(A.vulgaris) and Gaultheria fragrantissima(G.fragrantissima).Methods: Phytochemical screening, total phenolic and flavonoid content, antibacterial activities, anti-oxidant assay of the crude extract were carried out to identify the biological activities and phytonutrients present in the extract.Furthermore, the chemical constituents present in the essential oil and crude methanol extract were analyzed using gas chromatography mass spectroscopy and high performance liquid chromatography(HPLC) analysis.Results: Gas chromatography mass spectroscopy analysis of essential oil from the aerial part of A.vulgaris revealed 24 different compounds in it.Sabinene(11.29%), b-thujone(19.19%), chrysanthenone(4.48%), camphor(11.89%), borneol(4.44%) and germacrene D(8.42%) were the major compounds.Similarly, leaves of G.fragrantissima contained methyl salicylate(95%) and asarone(4.64%).Furthermore, methanol extract of leaves of A.vulgaris and G.fragrantissima were found rich in the total flavonoids and phenolic content.HPLC analysis of the methanol extract of leaves A.vulgaris revealed the presence of morin and luteolin, whereas rutin was found as a major flavonoids compound in the leaves of G.fragrantissima.Further, methanol extract of the A.vulgaris and G.fragrantissima showed the highest antioxidant and antibacterial properties compared to the essential oil.Conclusions: The HPLC analysis of the methanol extract of A.vulgaris shows the presence of luteolin and morin, whereas G.fragrantissima reveals the presence of rutin and a glycosylated flavonoids.Results reveal that A.vulgaris oil is the rich source of monoterpene and sesquiterpene compounds.Furthermore, A.vulgaris and G.fragrantissima are the rich source of the phenolic and flavonoids compounds and show good antioxidant and antibacterial activity.
基金Supported by National Key Technology Research and Development Plan(2015BAC05B02)Fundamental Research Funds for the Central Universities(2018NZD10)
文摘[Objectives] The aim was to study the chemical composition of the aboveground part of Artemisia subdigitata Mattf. [Methods]Using the systemic preparative test,the water extract,ethanol extract and petroleum ether extract of the aboveground part of A. subdigitata were subjected to physiochemical identification,and the contained chemical constituents were identified preliminarily. [Results] The preliminary comparisons of preparative test results of the aboveground part of A. subdigitata suggested that the whole plant may contain saponins,tannins,phenols,flavonoids,anthraquinones,coumarins,lactones,alkaloids,sterides or triterpenes,volatile oils and oils. [Conclusions]A. subdigitata contains a variety of chemical constituents. This study provides certain experimental basis for the further research and development and utilization of A. subdigitata.
文摘Artemisia vulgaris L. was collected from various regions in the USA and Montreal Canada. Gas Chromatography-Mass Spectrometry was used to identify the analytes present in the volatiles extracted by headspace solid-phase microextraction of the crushed leaves and flowers. Four distinct chemotypes are were found: One featuring the coexistence of ar-curcumene and α-zingiberene;two marked by the presence or absence of thujone and santolinatriene;and a fourth characterized by the presence of crysanthenyl acetate (40%). DNA was used to confirm the identity of Artemisia vulgaris L.
文摘Background: Artemisia afra is an indigenous plant used in Africa. It is used in traditional medicine to treat a variety of diseases caused by bacterial infections. As a result, developing innovative therapeutic methods using natural ingredients to combat pathogenic germs is critical. Methodology: Artemisia afra leaves were extracted using maceration extraction utilizing three solvents (petroleum ether, ethanol, and dichloromethane). Fresh leaves were extracted using hydrodistillation. The agar well diffusion method was used to test the crude extract and essential oil leaves of Artemisia afra against different human pathogenic bacteria strains (E. coli, S. aureus, E. faecalis, and K. pneumonia) at different concentrations in the presence of a positive (ciprofloxacin 5.0 μg) and negative (DMSO) control. Results: Artemisia afra revealed the presence of alkaloids, flavonoids, tannins, saponins, anthraquinones, terpenoids, coumarins, phenolic quinones, cardiac glycosides, and steroids. It showed that the highest antibacterial activity given by the ethanol extract had highly inhibition zones against gram-positive and gram-negative bacteria. The essential oil extract was effective against all tested bacteria. Conclusion: Plant crude extracts and essential oils may have antibacterial properties due to the synergistic activity of two or more active secondary metabolites.
基金supported by National Natural Science Foundation of China(81973444 and 81773875)National Key Research and Development Plan(2018YFC1707300).
文摘Background:As one of the most popular traditional Chinese medicines(TCMs)for the treatment of various liver diseases,virgate wormwood herb(Artemisia capillaris Thunb.)has a long application history in TCM practices.It has been well established that the chemical composition is responsible for the pronounced therapeutic spectrum of A.capillaris.Although they are comprehensive,the time-intensive liquid chromatography coupled to tandem mass spectrometry(LCeMS/MS)assays cannot fully satisfy the analytical measurement workload from many test samples.Direct infusion-MS/MS(DIeMS/MS)may be the optimal choice to achieve high-throughput analysis if the mass spectrometer can universally record MS2 spectra.Methods:According to the application of gas phase ion fractionation concept,the MS/MSALL program enables to gain MS2 spectrum for each nominal m/z value with a data-independent acquisition algorithm via segmenting the entire MS1 ion cohort into sequential ion pieces with 1 Da width,when sufficient measurement time is allowed by DI approach.Here,rapid clarification of the chemical composition was attempted for A.capillaris using DIeMS/MSALL.A.capillaris extract was imported directly into the electrospray ionization interface to obtain the MS/MSALL measurement.After the MS1-MS2 dataset was well organized,we focused on structural characterization through retrieving information from the available databases and literature.Results:Twenty-six compounds were found,including 12 caffeoyl quinic acid derivatives,7 flavonoids,and 7 compounds belonging to other chemical families.Among them,24 ones were structurally identified.Compared with the LCeMS/MS technique,DIeMS/MSALL has the advantages of low-costing,solvent-saving,and time-saving.Conclusions:Chemical profiling of A.capillaris extract was accomplished within 5 min by DIeMS/MSALL,and this technique can be an alternative choice for chemical profile characterization of TCMs due to its extraordinary high-throughput advantage.
文摘The aim of this study was to investigate the chemical composition of the essential oils of “Covid-Organics” of Madagascar (62% Artemisia annua and two other undisclosed medicinal plants) used as curative and preventive treatments of Covid-19, to identify its constituent species. The essential oils isolated by hydro-distillation from two samples (curative and preventive) were analyzed by GC-FID and GC-MS. These essential oils (curative and preventive) were mainly dominated by camphor (17.9% and 11.9%, respectively), spathulenol (4.8% and 11.8%, respectively), α-acorenol (4.3% and 3.7%, respectively), (E)-β-caryophyllene (3.4% and 4.2%, respectively), 1,8-cineole (3.1% and 3.6%, respectively), hexadecanoïc acid (3.8% and 3.2%, respectively) and caryophyllene oxide (3.4% and 2.4%, respectively). From the chemical composition, two species were identified, A. annua characterised by camphor and Cinnamomumcamphora (Ravintsara) characterised by 1,8-cineole and sabinene. However, we were unable to identify the third species.
基金Supported by the Research on Scientific and Technological Innovation of Beef Cattle in Northeast Cold Region by Ministry of Education(002020010)。
文摘Artemisia argyi is a traditional Chinese herb,which is widely distributed in various areas in China.In recent years,due to the development of A.argyi related industries and the continuous improvement of people’s health awareness,experts and scholars are concerned about the nutrients and effects of A.argyi.A.argyi is rich in carbohydrates,proteins,essential amino acids,fatty acids,minerals,vitamins,dietary fibers,flavonoids and other nutrients.It has the effects of antioxidant,anti-tumor,antibacterial,antiinflammatory,neuroprotection and immune regulations,and has been widely applied in the fields of COVID-19 prevention,food health care,food preservation and feed breeding,and has good development potential.This review summarized the research and application of A.argyi nutrients,in order to provide reference and application for further study.
文摘Malaria causes many deaths around the world, particularly in Africa, which ultimately affects the socio-economic development of African countries. The resistance of Plasmodium falciparum to quinine-based drugs led to new studies showing the efficiency of new artemisin-based drugs. The molecule artemisin is extracted from Artemisia annua a plant from China that has been used for decades in traditional Chinese medicine. The purpose of this study is to improve the production of sweet wormwood (Artemisia annua) using organic fertilizers in the north of Cote d’Ivoire. To do so, a morpho-pedological characterization of the study site was firstly performed to determine the soil type and their fertility level. Then, a randomized complete block system including two factors (the quantity of compost and the plant density) was implemented to test the effect of organic amendment and plant arrangement on the growth of Artemisia annua. Six treatments were set up: a control plot (no compost) where the plants are arranged in square (T0D1) and the plants are arranged in staggered (T0D2). Then, a treatment with compost addition of 25 t/ha where the plants are arranged in square (T1D1) and in staggered (T1D2). A treatment with compost addition of 50 t/ha where plants are arranged in square (T2D1) and in staggered (T2D2). Our results showed that the soils hosting our experimentation are Arenithic Plinthic Ferrasols with a very low level of fertility, prone to leaching and erosion. T1D2 and T2D2 treatments obtained the highest yields of 2.82 t/ha and 3.91 t/ha, respectively. Our findings indicate that a high dose of organic amendment combined with a staggered plant arrangement strongly improves the biomass production of sweet wormwood. This is in agreement with previous studies showing that the addition of organic matter can restore the level of soil fertility by increasing soil porosity and the activity of micro and macroorganisms.