Total oxidative stress, paraoxonase and arylesterase levels at patients with pseudoexfoliation syndrome and pseudoexfoliative glaucoma

AIMTo investigate the oxidative stress status of the aqueous humor and serum of patients with pseudoexfoliation (PEX) syndrome and pseudoexfoliative glaucoma (PEG) and to measure paraoxonase (PON) and arylesterase (ARE) levels.METHODSA total of 78 patients were enrolled in the study, with 26 patients in each separate group. The patients were divided into three groups: the first group entailed PEX syndrome patients, while the second group consisted of patients with PEG and the third group involved patients with no additional systemic diseases, other than the diagnosis of cataract as control. Total oxidative stress (TOS), total antioxidant capacity (TAC), PON, and ARE levels in aqueous humor and serum were measured.RESULTSTAC, PON and arylesterase levels in aqueous humor and serum of the PEX syndrome and PEG patients were significantly decreased compared with control group (P<0.05). TOS values were higher in patients with PEX syndrome and PEG than controls (P<0.05). TAC, PON and ARE levels of aqueous humor did not differ significantly between the PEX syndrome and PEG groupsCONCLUSIONThese findings are potentially of significance and add to the growing body of evidence for oxidative stress in PEX syndrome and PEG. Decreased antioxidant defense and increased oxidative stress system may play an important role in the pathogenesis of PEX syndrome and PEG.

Serum arylesterase and paraoxonase activity in patients with chronic hepatitis

AIM: To investigate the relationship between serum paraoxonase (PON1), AST, ALT, GGT, and arylesterase (AE) activity alterations and the degree of liver damage in patients with chronic hepatitis.METHODS: We studied 34 chronic hepatitis patients and 32 control subjects, aged between 35 and 65 years,in the Department of Infection and Clinical Microbiology at the Firat University School of Medicine. Blood samples were collected from subjects between 8:00 and 10:00 a.m. following a 12-h fast. Baseline and salt-stimulated PON1 activities were measured by the hydrolysis of paraoxon. Phenyl acetate was used as the substrate and formed phenol was measured spectrophotometrically at 270 nm after the addition of a 10-fold diluted serum sample in AE activity measurements.RESULTS: The results of this investigation revealed that the levels of AE activity decreased from 132±52 to 94±36 (29%), baseline PON1 activity from 452±112 to 164±67 (64%), salt-stimulated PON1 activity from 746±394 to 294±220 (61%), HDL from 58.4±5.1 to 47.2±5.6(20%), triglyceride from 133±51.2 to 86±34.0 (35%),while a slight increase in the level of LDL (from 163±54.1 to 177.3±56.0; 9%) and significant increases in the levels of AST (from 29±9.3 to 98±44), ALP (from 57.2±13.1 to 91±38.1), ALT (from 27.9±3.32 to 89±19.1), GGT (from 24.3±2.10 to 94±48.2), total bilirubin (from 0.74±0.02 to 1.36±0.06; 84%) and direct bilirubin (from 0.18±0.01 to 0.42±0.04; 133%) were detected.However, the levels of albumin, total protein, cholesterol,and uric acid were almost the same in chronic hepatitis and the control subjects.CONCLUSION: Low PON1 and AE activity may contribute to the increased liver dysfunction in chronic hepatitis patients by reducing the ability of HDL to retard LDL oxidation and might be clinically useful for monitoring the disease of chronic hepatitis.

基于CMA-ES的USV自动靠泊研究

本文提出基于CMA-ES优化的LQR船舶自主靠泊控制系统,系统化地解决船舶在靠泊过程中涉及的路径规划、最优控制、外界干扰、物理执行结构约束等问题并使用仿真软件实现基于LQR以及基于CMA-ES优化的LQR的自主靠泊控制系统,实验结果表明,基于CMA-ES的优化控制方法能够很好地实现小型船舶的自主靠泊,系统鲁棒性表现良好。

Fast estimation of Michaelis-Menten constant of arylesterase with a pair of medium concentrations of substrate

Objective: To investigate the reliability for fast estimation of Michaelis-Menten constant (Km) with calibrated specific activity at only two medium concentrations of substrate by both simulation and experimentation with arylesterase (ArE)as model. Methods: Initial rates were simulated by randomly inserting uniform absolute error, and the experimental initial rates of ArE were determined by measuring the increaser of product absorbance. Calibrated specific activities at two substrate concentrations were obtained by regression analysis, and Km was calculated according to Michaelis-Menten equation. Results: By simulation with calibrated specific activities at two medium substrate concentrations, Km could be calculated according to Michaelis-Menten equation with reasonable precision and accuracy. By experimentation with substrates of 2-naphthyl acetate, phenyl acetate, and p-nitrophenyl acetate, there were no differences between the mean and SD of Km of ArE for either substrate by this linear kinetic method and the Lineweaver-Burk plot. Conclusion: This linear kinetic method was reliable for fast estimation of the Km of some specified enzyme on its substrate of lower solubility or lower sensitivity for quantification by common methods.

ES纤维的应用与发展探讨

ES纤维是近几年非常热门的复合型纤维,主要用于热风无纺布的生产,是卫生巾、纸尿裤使用最广泛的材料。我国自发生重大公共卫生事件后,受N95口罩等的影响,热风无纺布成为热门产品,带动ES纤维迅猛发展。文章就ES纤维的发展历史、制作方法、特性等做了简单介绍,并对ES纤维非织造布技术的具体通用,以及国内ES纤维的生产现状进行了分析,对其发展方向和市场趋势做了初步探讨,以期各位读者对ES纤维有更多的了解。

一种评价新思维:ESE评价理论与方法——城市轨道交通可持续发展状态评价

目前城市轨道交通可持续发展评价体系,采用简单的分层加权汇总方法,未考虑同层指标间的相互影响以及下层指标对上层指标的一对多影响,不能系统、全面、准确地反映实际可持续发展情况。针对这一问题,本文提出了一种评价新思维——ESE评价理论与方法,即通过数据具有ESE(经济-社会-环境)三性影响这一新认识,构建了一个ESE空间直角坐标系,并以此表征城市轨道交通所有业务场景。在此ESE三维立体空间中,城市轨道交通的可持续发展状态被分成“八种四类”,同时对可持续发展程度进行了量化计算,并实现了立体的可视化表达。

Water, Sanitation and Hygiene in Lower-Level Health Care Facilities of Dar es Salaam Region in Tanzania: Status towards Achieving the Sustainable Development Goals and Way Forward

Background: Improving Water, Sanitation and Hygiene in health care settings is a critical prerequisite for achieving national health goals and Sustainable Development Goals (SDGs). The World Health Organization (WHO) has set a target for each United Nations member state to reach by 2030. Each member state is required to reach by 2022, 2025 and 2030 at least 60%, 80% and 100%, respectively of basic level of service of the five elements which are water, sanitation, hygiene, waste management and environmental cleaning. Methods: This study aimed to evaluate and document the current state of basic water, sanitation, and hygiene services in all lower-level health care facilities in the Dar es Salaam region of Tanzania as of July 2022. A cross-sectional study was conducted in 99 public dispensaries in the Dar es Salaam region’s five councils: Ubungo, Kigamboni, Kinondoni and Temeke Municipalities, and Ilala City. The interviewee form and observational checklists were both digitalized using the Kobo tool software. The respondents were health care facility in-charges or nurse in-charges. Data were downloaded, validated, and imported to Stata version 15 for analysis. Results: The basic WASH level per JMP is far below the target in 2022. Each member state by 2022 is required to reach at least 60% of the basic level of service of each element. We found a low coverage of basic WASH in the 99 dispensaries included in this study. The basic WASH coverage was met in only 10 (10.1%) of the dispensaries, while the remaining 89 (89.9%) dispensaries fall on limited WASH services. Conclusion: This study revealed lower coverage of basic WASH services in dispensaries. An urgent need is required to improve the status of WASH in all the dispensaries and facilitate the provision of quality health care services, patient safety and reduce health care associated infections.

嵌入式系统OpenGLES提升帧率的批量渲染方法的研究

在嵌入式系统中使用OpenGL ES进行渲染工作,往往会受限于纹理渲染帧率。为了提高帧率,业内已有一些改进的方法,如VBO、PBO、压缩纹理等。从影响OpenGL ES渲染速率较大的2个API(glDraw*,glTexImage2D)入手,提出多纹理单元与Sprite Widget 2种Batch Rendering方法,并附有测试数据,验证了这2种方法在帧率提升方面具有较好效果。

Correlation of serum arylesterase activity on phenylacetate estimated by the integrated method to common classical biochemical indexes of liver damage

The correlation of serum arylesterase(PON1) activity on phenylacetate determined by an integrated method to clas-sical biochemical indexes of liver damage was investigated for the use of PON1 activity to evaluate liver damage.PON1 reaction curve as absorbance at 270 nm for 0.20 mmol/L phenylacetate hydrolysis was analyzed by the integrated method to determine maximal PON1 reaction rate.Classical biochemical indexes of liver damage were determined routinely.The 95% confidence threshold of PON1 activity in sera from healthy individuals was 2.12 mkat/L [(4.73±1.31) mkat/L,n=105].PON1 activity in clinical sera was closely correlated to serum albumin,total protein and the ratio of albumin to globulins,but was weakly correlated to both direct and total bilirubin in serum.There were no correlations of PON1 activity to γ-glutamyltransferase,alkaline phos-phatase,alanine aminotransferase and aspartate aminotransferase.Among 127 clinical sera with PON1 activity>2.12 mkat/L,there were 92% healthy individuals examined by albumin,90% healthy individuals examined by total protein,88% healthy individuals examined by total bilirubin,86% healthy individuals examined by direct bilirubin and 64% healthy individuals examined by the ratio of albumin to globulins,respectively.In each group of healthy individuals judged by classical biochemical indexes of close correlation to PON1 activity,percentage of healthy individuals examined by PON1 activity was always >80%.These results suggested PON1 activity on phenylacetate estimated by the integrated method was also suitable for the evaluation of liver damage.

细旦ES纤维的生产工艺探讨

ES纤维是两种不同的高分子材料通过熔融纺丝复合而成的纤维,细旦ES纤维是指线密度达到1 dtex以下的ES纤维。由于细旦ES纤维的极限性,文章主要根据ES纤维的生产流程,讨论细旦ES纤维的生产工艺,对影响细旦ES纤维生产的纺丝连续性与拉伸连续性的因素展开研究,最后指出有利于细旦ES纤维稳定生产的工艺调整方向。

ES热风无纺布打孔工艺及设备探讨

为探索热风无纺布的打孔工艺,文章分析了热风无纺布的结构,介绍热风无纺布的主要用途及对打孔的要求,简述了打孔无纺布与平纹无纺布的主要差异,基于在线打孔热风无纺布的现状和特点,详细研究了离线打孔无纺布的加工方法,旨在为热风无纺布打孔的新方法和工艺提供最新的思路。

东风富康ES600空调系统不制冷

车型:东风富康ES600纯电动车。VIN:LDCD730E1N3xxxxxx。行驶里程:82000km。故障现象:车辆的空调系统突然发生持续性不制冷的情况,此时中控屏上显示的室外温度和实际相符,没有异常。

东风富康ES600偶发自动挂N挡故障

车型:东风富康ES600。VIN:LDCD730E8N3××××××。行驶里程:213249km。故障现象:车辆在最近两个月的使用过程中,到每天晚上19点钟以后车辆缓行或等红灯的情况下,会不定时出现车辆自动挂N挡的不正常情况。故障诊断:先与客户进行沟通,了解车辆的使用情况及保养等相关信息,车辆此前无任何碰撞及事故维修。由于是客户购买后进行网约车运行的,所以按规定加装网约车GPS,同时也进行了改装近光灯(网购)。全车除这两个项目外无任何其他改装/加装电器和相关电器线路改装。

Strategies for Fostering Interaction in Online Classrooms:A Conversation Analysis of Teacher-Student Verbal Interaction in Random Questioning in Pandemic-Initiated Online Teaching

This paper aims to explore how a veteran teacher organizes online teaching initiated by the pandemic and how she deals with the problems in online teacher-student verbal interaction.By analyzing a corpus of 20 audio-recorded online lessons between a math teacher and her students during the COVID-19 pandemic from April 11 to May 10,2022,four interactional segments are selected as the focus of the study.The results of the conversation analysis of the segments showed that students’modesty,lack of confidence,lack of ability,and network delay are the main factors affecting online teacher-student interaction.By encouraging students to answer questions,enlightening students to give answers,enriching students’answers,and entertaining the teaching atmosphere(“4Es”strategies),the teacher solved the problems successfully.The findings from this study can provide pedagogical experience and implications for practical teaching.

基于区块链和ES搜索引擎的特高压工程设计数据共享技术

本文提出一种基于区块链和ES搜索引擎的特高压工程设计数据共享技术,该技术利用区块链验证了数据的真实性,建立了基于ES搜索引擎的数据全文检索机制,按照工程职责分工定义了数据共享权限,研发了在线共享系统,为多源设计数据统一管理与共享调阅提供了一种新思路。

东风富康ES600行驶过程中突然无法加速

车型:东风富康ES600(充电版)。VIN:LDCD730E1N×××××××。行驶里程:91143km。故障现象:客户反映车辆正常行驶时组合仪表突然显示:电子牵引力系统故障、速度受限、请维修车辆等故障信息,同时车辆行驶功率限制指示灯点亮(乌龟灯),车辆处于降功率运行状态,无法正常加速。故障诊断:客户将车辆开到网点后,我们先与客户进行沟通,了解车辆的使用情况及保养等相关信息,车辆此前无任何碰撞及事故维修,全车无任何改装/加装电器和相关电器线路改装。车辆组合仪表上的故障显示信息情况如图1所示。

基于实例分析ESE避雷针在海外光伏电站中的应用

大型光伏电站由于占地面积大且多位于空旷多雷区,易遭受雷击。分析了光伏电站雷击危害,针对海外光伏发电项目,提出在光伏场区采用预放电(ESE)避雷针的方法,ESE避雷针具有体积小、阴影小、保护半径大等优点。结合实际光伏电站,提出了采用ESE避雷针时的设计方案,比较了ESE避雷针方案和常规避雷针方案的经济性。实际运行结果表明:ESE避雷针可以规范、有效、经济的保证光伏电站的安全运行。

2019年别克GL8 ES驻车制动无法工作

车型:2019年别克GL8 ES,配置LTG 2.0T发动机。VIN:LSGUL83L4KA××××××。行驶里程:30948km。故障现象:客户反映前一段时间出现过车辆停车熄火后无法驻车制动现象,并伴随仪表上驻车制动器故障灯闪烁现象,但有些时候可以正常使用。这次出现此现象后就没有正常使用过驻车制动器。

基于OpenGL ES的图像滤波算法实现及优化研究

图像滤波算法广泛应用在机器学习、图像处理、图像识别等领域,在降低椒盐噪声、图像二值化、边缘识别和特征提取等方面发挥着重要作用。现有图像滤波算法虽然在OpenCV开源库中均有实现,但是在Android平台的性能表现与其他平台相比仍存在较大差距。随着嵌入式平台的高速发展,实际应用对嵌入式平台上滤波算法的性能要求越来越高。为此,从形态学滤波、盒式滤波、阈值滤波、压缩滤波、算术滤波等具有广泛应用场景的滤波算法入手,提出并实现针对Android平台设计、基于OpenGL ES的一系列高性能图像滤波算法。使用OpenGL ES计算着色器对算法进行并行加速,使用纹理对象进行访存优化,并在图像边界处理、图像数据类型和数据通信等方面进行深入优化,取得了较优的性能。将优化的图像滤波算法与开源OpenCV库中对应算法进行对比,实验结果表明,基于Android平台使用OpenGL ES接口的图像滤波算法整体性能明显优于OpenCV库中的相关算法,且图像规模越大计算优势越明显,性能最大提升为OpenCV库中对应算法的110.018倍。

中国区域电离层Es与太阳活动相关性分析

利用1958-2018年国内4台站电离层数据,研究了Es与太阳活动的相关性.结果表明,Es与太阳活动的相关性随季节和地方时不同而异.在冬分季白天,各台站Es与太阳活动为强的正相关,而夏季夜间为强的负相关,其他时段不同台站有所差异.满洲里和北京站在冬分季白天foEs中值与R12拟合系数为0.002,广州和海口站则为0.003.在夏季夜间分别为-0.002和-0.004。金属离子形成的Es在夏季白天出现较多,冬分季白天较少.此外,Es全遮蔽出现率P和半遮蔽因子Fb与太阳活动负相关,且呈现出二阶特性。