Factors Contributing to Contamination of Street Foods in Bamako, Mali

The World Health Organization states that foodborne diseases are a worldwide public health issue. Although street foods can provide nutritious and affordable ready-to-eat meals for city dwellers, their health risks can outweigh the benefits. A cross-sectional study was conducted in the Bamako district, focusing on street food vendors near schools, universities, extensive markets, administrative centers, and major roads. We aimed to sample fifty (50) sellers per municipality, making 300 sellers for the Bamako district. We developed a survey sheet to collect data, and six teams rotated between the municipalities each month. Before starting the collection, the teams were provided administrative papers approved by the municipal authority. The survey revealed three types of sales sites: fixed (65%), semi-fixed (30%), and mobile (4.40%). The proportion of sellers was 26.8%, 23.2%, 19.7%, and 4.2% in municipalities III, IV, and I. In municipalities I, II, III, IV, and VI, respectively, 92%, 95.70%, 93%, 87.2%, and 100% of the sellers were female. The age distribution of sellers was 65.63%, 46.81%, 40.82%, 38.30%, 36.17%, 36%, and 32% in the 25-34 and 35 - 44 age groups. Illiteracy rates were 59.20%, 61.70%, 55.30%, 75%, and 56% in municipalities I, II, III, IV, and VI, respectively. The study identified two categories of sellers: 48.3% in type 1 and 51.7% in type 2. The first category comprised 154 sellers, and the second 165 sellers. The survey found that 66.00%, 56.00%, 48.90%, 44.90%, 38.30%, and 34.40% of municipal V, VI, III, I, II, and IV sales sites were open-air. In municipality I, 63.30% of the sites were under hangars, while in municipalities II and IV, the corresponding percentages were 51.10% and 59.40%, respectively. Moreover, 46.00%, 31.90%, 31.30%, 30.60%, and 27.70% of the sites in municipalities VI, II, IV, I, and III were located next to gutters. In conclusion, this study identified several factors that could compromise the quality of street foods sold in the six municipalities of Bamako.

Ultrasonic enhancement of persulfate oxidation system governs emerging pollutants decontamination

Emerging contaminants (ECs) are widely present in aquatic environments, posing potential risks to both ecosystems and human health. Theultrasound-assisted persulfate oxidation process has attracted considerable attention in the degradation of ECs due to its ability to generate bothsulfate radicals and cavitation effects, enhancing degradation effects. In this paper, the principle of ultrasonic synergistic Fenton-like oxidationsystem for degrading organic pollutants was reviewed, divided into homogeneous system, non-homogeneous system, and single-atom system toexplore the synergistic effect of ultrasound-enhanced persulfate technology in three aspects, and the effects of environmental factors such asultrasonic frequency and power, system pH, temperature, and initial oxidant concentration on the system's decontamination performance werediscussed. Finally, future research on ultrasonically activated persulfate technology is summarized and prospected.

Phytoremediation Strategies for Heavy Metal Contamination: A Review on Sustainable Approach for Environmental Restoration

Current globalization trends and important breakthroughs globally need a complete study of heavy metal contamination, its causes, its impacts on human and environmental health, and different remediation strategies. Heavy metal pollution is mostly produced by urbanization and industry, which threatens ecosystems and human health. Herein, we discuss a sustainable environmental restoration strategy employing phytoremediation for heavy metal pollution, the carcinogenic, mutagenic, and cytotoxic effects of heavy metals such as cadmium, copper, mercury, selenium, zinc, arsenic, chromium, lead, nickel, and silver, which may be fatal. Phytoremediation, which was prioritized, uses plants to remove, accumulate, and depollute pollutants. This eco-friendly method may safely collect, accumulate, and detoxify toxins using plants, making it popular. This study covers phytostabilization, phytodegradation, rhizodegradation, phytoextraction, phytovolatilization, and rhizofiltration. A phytoremediation process’s efficiency in varied environmental circumstances depends on these components’ complex interplay. This paper also introduces developing phytoremediation approaches including microbe-assisted, chemical-assisted, and organic or bio-char use. These advancements attempt to overcome conventional phytoremediation’s limitations, such as limited suitable plant species, location problems, and sluggish remediation. Current research includes machine learning techniques and computer modeling, biostimulation, genetic engineering, bioaugmentation, and hybrid remediation. These front-line solutions show that phytoremediation research is developing towards transdisciplinary efficiency enhancement. We acknowledge phytoremediation’s promise but also its drawbacks, such as site-specific variables, biomass buildup, and sluggish remediation, as well as ongoing research to address them. In conclusion, heavy metal pollution threatens the ecology and public health and must be reduced. Phytoremediation treats heavy metal pollution in different ways. Over time, phytoremediation systems have developed unique ways that improve efficiency. Despite difficulties like site-specificity, sluggish remediation, and biomass buildup potential, phytoremediation is still a vital tool for environmental sustainability.

Exploring the mechanical behavior and microstructure of compacted loess subjected to dry-wet cycles and chemical contamination

Due to climatic factors and rapid urbanization,the soil in the Loess Plateau,China,experiences the coupled effects of dry-wet cycles and chemical contamination.Understanding the mechanical behavior and corresponding microstructural evolution of contaminated loess subjected to dry-wet cycles is essential to elucidate the soil degradation mechanism.Therefore,direct shear and consolidation tests were performed to investigate the variations in mechanical properties of compacted loess contaminated with acetic acid,sodium hydroxide,and sodium sulfate during dry-wet cycles.The mechanical response mechanisms were investigated using zeta potential,mineral chemical composition,and scanning electron microscopy(SEM)tests.The results indicate that the mechanical deterioration of sodium hydroxidecontaminated loess during dry-wet cycles decreases with increasing contaminant concentration,which is mainly attributed to the thickening of the electrical double layer(EDL)by Nat and the precipitation of calcite,as well as the formation of colloidal flocs induced by OH,thus inhibiting the development of large pores during the dry-wet process.In contrast,the attenuation of mechanical properties of both acetic acid-and sodium sulfate-contaminated loess becomes more severe with increasing contaminant concentration,with the latter being more particularly significant.This is primarily due to the reduction of the EDL thickness and the erosion of cement in the acidic environment,which facilitates the connectivity of pores during dry-wet cycles.Furthermore,the salt expansion generated by the drying process of saline loess further intensifies the structural disturbance.Consequently,the mechanical performance of compacted loess is sensitive to both pollutant type and concentration,exhibiting different response patterns in the dry-wet cycling condition.

Long-term hydrochemical monitoring and geothermometry:understanding groundwater salinization and thermal fluid contamination in Mila’s basin,Northeastern Algeria

The regular hydrochemical monitoring of groundwater in the Mila basin over an extended period has provided valuable insights into the origin of dissolved salts and the hydrogeochemical processes controlling water salinization.The data reveals that the shallow Karst aquifer shows an increase in TDS of 162 mg L^(-1) while the ther-mal carbonate aquifer that is also used for drinking water supply exhibits an increase of 178 mg L^(-1).Additionally,significant temperature variations are recorded at the sur-face in the shallow aquifers and the waters are carbo-gaseous.Analysis of dissolved major and minor elements has identified several processes influencing the chemical composition namely:dissolution of evaporitic minerals,reduction of sulphates,congruent and incongruent car-bonates’dissolution,dedolomitization and silicates’weathering.The hydrogeochemical and geothermometric results show a mixing of saline thermal water with recharge water of meteoric origin.Two main geothermalfields have been identified,a partially evolved water reservoir and a water reservoir whosefluid interacts with sulphuric acid(H_(2)S)of magmatic origin.These hot waters that are char-acterized by a strong hydrothermal alteration do ascend through faults and fractures and contribute to the contamination of shallower aquifers.Understanding the geothermometry and the hydrogeochemistry of waters is crucial for managing and protecting the quality of groundwater resources in the Mila basin,in order to ensure sustainable water supply for the region.A conceptual model for groundwater circulation and mineralization acquisition has been established to further enhance under-standing in this regard.

Investigation of an electrode-driven hydrogen plasma method for in situ cleaning of tin-based contamination

To prolong the service life of optics,the feasibility of in situ cleaning of the multilayer mirror(MLM)of tin and its oxidized contamination was investigated using hydrogen plasma at different power levels.Granular tin-based contamination consisting of micro-and macroparticles was deposited on silicon via physical vapor deposition(PVD).The electrodedriven hydrogen plasma at different power levels was systematically diagnosed using a Langmuir probe and a retarding field ion energy analyzer(RFEA).Moreover,the magnitude of the self-biasing voltage was measured at different power levels,and the peak ion energy was corrected for the difference between the RFEA measurements and the self-biasing voltage(E_(RFEA)-eV_(self)).XPS analysis of O 1s and Sn 3d peaks demonstrated the chemical reduction process after 1 W cleaning.Analysis of surface and cross-section morphology revealed that holes emerged on the upper part of the macroparticles while its bottom remained smooth.Hills and folds appeared on the upper part of the microparticles,confirming the top-down cleaning mode with hydrogen plasma.This study provides an in situ electrode-driven hydrogen plasma etching process for tin-based contamination and will provide meaningful guidance for understanding the chemical mechanism of reduction and etching.

Assessment of Heavy Metals Contamination of Topsoil and Street Dust around Cement Factory in Southern Jordan

Evaluation of assessment of the metal processes governing the metals distribution in soil and dust samples is very significant and protects the health of human and ecological system. Recently, special attention has given to the assessment of metals pollution impact on soil and dust within industrial areas. This study aims to assess the metal contamination levels in the topsoil and street dust around the cement factory in Qadissiya area, southern Jordan. The levels of seven metals (namely Fe, Zn, Cu, Pb, Cr, Cd, and Mn) were analyzed using Flame Atomic Absorption Spec-trophotometer (FAAS) to monitor, evaluate, and to compare topsoil and road dust pollution values of metals of the different types of urban area. The physicochemical parameters which believed to affect the mobility of metals in the soil of the study area were determined such as pH, EC, TOM, CaCO3 and CEC. The levels of metal in soil samples are greater on the surface but decrease in the lower part as a result of the basic nature of soil. The mean values of the metals in soil can be arranged in the following order: Zn > Pb > Mn > Fe > Cu > Cr > Cd. The relatively high concentration of metals in the soil sample was attributed to anthropogenic activities such as traffic emissions, cement factory and agricultural activities. Correlation coefficient analysis and the spatial distribution of indices and the results of statistical analysis indicate three groups of metals: Fe and Mn result by natural origin, Zn, Pb, Cu and Zn result by anthropogenic origin (mainly motor vehicle traffic and abrasion of tires) while Cd is mixed origin. The higher content level values of metals of anthropogenic source in soil samples indicate that it is a source of contamination of air in the studied area. .

Evaluating bacterial contamination and surgical site infection risks in intracorporeal anastomosis: Role of bowel preparation

We recently read the study by Kayano et al on intracorporeal anastomosis(IA)for colon cancer,which assessed bacterial contamination and medium-term onco-logical outcomes and affirmed that IA is analogous to extracorporeal anastomosis in reducing intraperitoneal bacterial risk and achieving similar oncological results.Our commentary addresses gaps,particularly concerning bowel preparation and surgical site infections(SSIs),and highlights the need for comprehensive details on the bowel preparation methods that are currently employed,including mecha-nical bowel preparation,oral antibiotics(OA),their combination,and specific OA types.We emphasize the necessity for further analyses that investigate these me-thods and their correlation with SSI rates,to enhance clinical protocol guidance and optimize surgical outcomes.Such meticulous analyses are essential for refi-ning strategies to effectively mitigate SSI risk in colorectal surgeries.

Study on Preservation of Boar Semen and Bacteria Contamination in Kandal Province

The breeding program is progressing steadily,with increasing the awareness among farmers regarding the its critical role in improving productivity,promoting rapid growth,enhancing disease resistance,and boosting household income.At the same time,the dissemination of AI(Artificila Insemination)technique among farmers has been active and effective.For the application of the AI,the quality of boar semen is extended in BTS(Belts Ville Thawing Solution)for more longer preservation period.The study purpose is to evaluate sperm survival time and bacterie contamination in boar semen.Six boars with different breed around 3 years old were used for this study as two Durocs,two Landraces and two Yorkshires.In evaluation sperm survival time in extender on each boar was designed by CRD(Completely Randomized Design)and calculating the survival time of spermatozoa was done after semen dilution and the sperm mobility was determined.The experimental BTS compositions are glucose,sodium bicarbonate acid,trisodium citrate,ethylenediaminetetraacetic acid,potassium chloride and distill water.In the same time,eighteen sample semen ware collected and used for detection of bacterial contamination.The results showed that the survival time of spermatozoa with its straight mobility more than 50%between the commercial BTS and BTS-KH was from 52 to 56 h.They are not significantly different(p>0.05).And the total survival time of spermatozoa was from 92 to 98 h,they are also not significantly different(p>0.05).The survival time of spermatozoa with its straight mobility more than 50%between the Yorkshire,Landrace and Duroc was 55 h 5 min,54 h 25 min and 50 h 18 min respectively.They are significantly different(p>0.05).The total survival time of spermatozoa between the commercial BTS and BTS-KH was 97 h 19 min,91 h 29 min and 92 h 26 min respectively.They are also not significantly different(p>0.05).The main bacteria isolated from the diluted semen were gram-negative bacteria,Escherichia coli 27.78%was contaminated with an average 6.4×106 CFU/mL,and the family Pseudomonaceae was 11.11%with 8.3×106 CFU/mL.In conclusion,the comparative results of the survival time of spematozoa during the breeding period(t=0.5)between the breeds and extender are similar.For bacteria contamination isolate from the semen all breeds may be due to direct and environmental factors.It is a rainy season with heavy rains that can cause infections.

Agricultural Contamination of the Surface Waters of the Upper Ouémé in Benin: The Case of Heavy Metals and Pesticides

Objective: The main objective of this study was to assess the degree of contamination of surface waters by heavy metals and pesticides. Method: To this end, data were collected in December 2022 from four specific sampling stations: Okpara, Térou, Affon and Adjiro. Levels of heavy metals, including cadmium, chromium, copper, iron, mercury, nickel and lead, were measured and subjected to in-depth statistical analysis using graphical summation models. In addition, the concentrations of pesticide active ingredients present in the samples were interpreted and evaluated. The statistical data collected during this study were processed using R software, version 3.5.0. Results: The values obtained at the different stations Okpara, Térou, Affon and Adjiro are respectively Arsenic (2 × 10-4 mg/L;2.2 × 10-1 mg/L;1.2 × 10-4 mg/L;2 × 10-4 mg/L), Cadmium (4.4 × 10-5 mg/L;1.1 × 10-2 mg/L;10-4 mg/L;4 × 10-4 mg/L). Then Copper (7 × 10-4 mg/L;3 × 10-3 mg/L;7 × 10-4 mg/L;1 × 10-4 mg/L), Iron (1.51 mg/L;6.4 × 10-1 mg/L;2.0012 mg/L;2.9 × 10-1 mg/L), Lead (0 mg/L;0 mg/L;1.5 × 10-3 mg/L;1.5 × 10-3 mg/L). Mercury, nickel and chromium were not detected in surface waters. It is important to note that the values obtained for trace metals (Cadmium, Chromium, Copper, Iron, Mercury, nickel and chromium were not detected in surface waters. It is important to note that the values obtained for trace metals (cadmium, chromium, copper, iron, mercury, nickel and lead) were all below the guideline standards set by the WHO in 2006 for uncontaminated surface waters. This indicates that the surface waters of the Upper Ouémé were below acceptable contamination thresholds in terms of heavy metals. However, the presence of pesticide active ingredients such as cyfluthrin, endosulfan-alpha, endosulfan-beta, profenosfos, tihan, atrazine, gala super and glycel clearly indicates that these surface waters are subject to agricultural contamination.

微针导入重组Ⅲ型人源化胶原蛋白在皮肤屏障功能修复中的应用效果

目的探究微针导入重组Ⅲ型人源化胶原蛋白治疗因皮肤炎性衰老导致的皮肤屏障功能下降的有效性及安全性。方法选取2020年6月至2021年5月在本院接受面部皮肤治疗的30例患者作为研究对象,采用随机数字表法将其分为对照组(重组Ⅲ型人源化胶原蛋白)与治疗组(微针导入重组Ⅲ型人源化胶原蛋白),各15例。治疗后定期对患者进行随访,分别进行主观疗效评价及客观疗效评价。结果治疗后4、8、12周,治疗组的满意度显著高于对照组(P<0.05)。治疗后,治疗组的皮肤皱纹、纹理、红区、毛孔、弹性、皮肤经皮失水及皮肤含水量改善明显(P<0.05);对照组治疗后油脂改善明显(P<0.05)。治疗后12周,治疗组的总症状评分显著低于对照组(P<0.05)。结论微针导入重组Ⅲ型人源化胶原蛋白对皮肤修复有较好的疗效,可增强皮肤屏障功能,为改善皮肤屏障功能、治疗因皮肤屏障受损引起的炎性衰老提供了新选择。

基于CatBoost-NSGA-Ⅲ算法的盾构姿态预测与优化

为解决盾构掘进过程中因盾构前倾变形、蛇形、轴线偏离及纠偏等影响施工安全性与高效性的问题,提出一种将类别型特征梯度提升(CatBoost)与第三代非支配排序遗传算法(NSGA-Ⅲ)相结合的盾构姿态多目标优化方法;以贵阳地铁为例,选取22个影响因素作为输入参数,利用CatBoost算法建立输入参数与盾构姿态之间的非线性映射函数关系,采用随机森林(RF)算法评价输入参数的重要性;以盾构姿态绝对值最小化为目标,构建CatBoost-NSGA-Ⅲ多目标优化模型,并通过案例分析验证所提方法的适用性和有效性。结果表明:采用CatBoost算法训练工程实测数据得到的预测模型具有较高的精度,5个盾构姿态目标的R^(2)范围为0.916~0.943;所研发的CatBoost-NSGA-Ⅲ盾构姿态多目标优化方法,可使盾构姿态得到显著优化,整体改进的平均值为53.34%。

水稻Ⅲ类过氧化物酶基因IPH1调控水稻株高

过氧化物酶(peroxidase, PRX)是过氧化物酶体的标志酶,能够保护细胞免受氧化损伤和解除H2O_(2)的毒害作用,还可以增强自然杀伤细胞的活性,调节细胞的增殖、分化和凋亡等.其中Ⅲ类过氧化物酶(CⅢPRXs)是植物中特有的过氧化物酶家族,通过清除活性氧(ROS)在植物免疫中发挥重要作用,然而CⅢPRXs在水稻株型建立中的功能尚不清楚.通过基因编辑技术CRISPR/Cas9获得CⅢPRXs基因(LOC_Os12g09460)两种不同形式的敲除突变体iph1-1,iph1-2.iph1突变体的株高显著高于野生型,除倒1节节长(即第5节)外,其他节节长均显著或极显著长于野生型.农艺性状考察表明,穗长及结实率等主要性状差异无统计学意义;通过RT-qPCR技术进行的表达模式分析表明,IPH1在根、茎、叶、鞘、穗中均表达,并在茎和鞘中表达相对较高;亚细胞定位分析表明,IPH1蛋白主要定位于过氧化物酶体中.进一步通过生理分析,发现突变体中过氧化物酶活性显著降低,同时H2O_(2)质量分数显著增加.这些结果初步证实了IPH1能够通过过氧化氢途径调控水稻株高的形成,可为丰富株高调控网络提供有利的基因资源,进一步为株型相关生物育种奠定基础.

改良前方牵引矫治器治疗骨性Ⅲ类错[牙合]伴拥挤的疗效评价

目的:评价改良前方牵引矫治器治疗骨性Ⅲ类错[牙合]伴拥挤的疗效。方法:将40例骨性Ⅲ类错[牙合]患者,按磨牙关系分为2组,每组20例,试验组磨牙中性或远中关系,应用改良前方牵引矫治器;对照组磨牙近中关系,应用传统前方牵引矫治器。2组患者治疗前后分别拍摄头颅侧位片进行比较,采用SPSS 22.0软件包对数据进行统计学分析。结果:2组患者治疗前后角度测量中,SNA、ANB、SN-MP、U4-SN显著增加(P<0.01),SNB显著减小(P<0.01);SN-OL在试验组治疗前后变化有显著差异(P<0.05)。2组患者治疗前后矢状向测量指标中,除对照组上颌牙弓长度外,其余指标均有显著改变(P<0.05);垂直向测量指标中,U1-PP、L1-MP、U4-SN、U6-SN、ANS-Me显著增加(P<0.05);U4-PP、U6-PP在对照组治疗前后的变化有显著差异(P<0.05)。2组患者治疗前后头影测量值变化量与对照组相比,试验组治疗后上颌牙弓长度显著增加,U6位置更偏远中,磨牙关系变化更小(P<0.01)。结论:改良前方牵引矫治器对磨牙中性或远中关系的骨性Ⅲ类错[牙合]伴拥挤患者具有良好的前方牵引和推磨牙向远中的效果。

以箬叶“炭”究Cr(Ⅲ)和Cr(Ⅵ)的检测与吸附综合实验

本文以“科教融合”为导向,以湖北鹤峰的箬叶为原料,制备生物质炭和碳量子点并进行基本表征,以生物质炭吸附Cr(Ⅲ)和Cr(Ⅵ),以荧光淬灭法检测Cr(Ⅲ)和Cr(Ⅵ),吸附和检测过程绿色环保。结果表明生物质炭吸附Cr(Ⅲ)的吸附符合颗粒内扩散模型,吸附Cr(Ⅵ)符合准二级动力学模型,二者均为自发吸热过程。作为本科生综合教学实验,本实验不仅涉及多种无机化学、分析化学、物理化学、仪器分析的理论知识,还包含了制备、表征、数据处理、吸附机制分析等重要环节,同时融入了文化自信、地域文化和绿色化学等课程思政理念。该创新实验教学时长约为24小时,适合用于综合化学实验教学,实现了基础化学理论在实践中的有效应用,提升了化学专业学生的综合能力。

P507溶剂浸渍树脂吸附Ga(Ⅲ)的性能研究

溶剂浸渍树脂(SIRs)分离法兼具溶剂萃取与离子交换两种分离方法的优点,且树脂制备简单,可根据实际情况灵活选用不同的载体和萃取剂,有望实现镓绿色、高效的分离回收。本文以苯乙烯-二乙烯基苯大孔吸附树脂(代号:HZ818)为载体、2-乙基己基膦酸单2-乙基己基酯(代号:P507)为萃取剂,制备P507 SIRs,研究了该SIRs在H 2SO 4体系中吸附Ga(Ⅲ)的性能。静态吸附实验表明:298 K时,P507 SIRs对Ga(Ⅲ)的最佳吸附pH值为3.3,对应吸附量为13.7 mg·g^(-1);吸附平衡时间为14 h,动力学吸附过程可用拟二级动力学方程描述;等温吸附均符合Langmuir等温吸附模型。动态吸附实验表明:低流速有利于P507 SIRs对Ga(Ⅲ)充分吸附;1.5 mol·L^(-1)的HCl可以将吸附于P507 SIRs上的Ga(Ⅲ)完全洗脱;P507 SIRs对Ga(Ⅲ)循环吸附稳定性能有待提高。在Ga(Ⅲ)-Zn(Ⅱ)、Ga(Ⅲ)-Cu(Ⅱ)二元混合体系中,P507 SIRs对Ga(Ⅲ)具有较好的吸附选择性,通过控制溶液pH值可以实现Ga(Ⅲ)的分离富集;在含Fe(Ⅲ)的Ga(Ⅲ)-Fe(Ⅲ)二元体系和Ga(Ⅲ)-Zn(Ⅱ)-Cu(Ⅱ)-Fe(Ⅲ)多元体系中,P507 SIRs对Fe(Ⅲ)表现出较好的吸附选择性,而对Ga(Ⅲ)吸附甚微,表明Fe(Ⅲ)的存在对P507 SIRs吸附Ga(Ⅲ)产生较大干扰。

复方黄柏液涂剂促进Ⅲ度烧伤创面肉芽组织形成的作用机制研究

目的:基于网络药理学和分子对接方法,确定复方黄柏液治疗的Ⅲ度烧伤肉芽组织愈合的有效活性成分、关键靶点和潜在的药理学机制,并进行肉芽组织成纤维细胞的初步验证。方法:从公共数据库中药系统药理学分析平台(TCMSP)检索复方黄柏液组成成分连翘、黄柏、金银花的有效成分和靶点;GeneCards、OMIM数据库检索“Ⅲ度烧伤”疾病相关靶点。通过生物信息学分析,包括蛋白质-蛋白质相互作用(Protein-proteininteraction,PPI)以及基因本体(Gene ontology,GO)和京都基因和基因组百科全书(Kyoto encyclopedia of genes and genomes,KEGG)分析,获得了关键的有效成分、核心靶点和相关信号通路;DiscoveryStudio分子对接分析有效成分化合物与靶蛋白的结合。0.5%的DMSO溶液处理的成纤维细胞记为对照组;槲皮素(40μmol/ml)处理的成纤维细胞记为槲皮素组。采用CCK8法、Transwell实验检测细胞增殖、迁移侵袭;WB试验检测细胞p-PI3K、p-Akt蛋白。结果:共筛选出74个有效成分,331个作用靶点,AKT1为潜在的治疗靶点,木犀草素、山柰酚、槲皮素、汉黄芩素、丹皮酚为潜在的候选药物。PI3K-AKT信号通路可能在复方黄柏液治疗Ⅲ度烧伤中发挥关键作用;分子对接表明槲皮素与AKT1结合最好。与对照组相比,槲皮素组成纤维细胞增殖、迁移侵袭均显著降低,p-PI3K、p-Akt蛋白表达也显著降低(P<0.05)。结论:复方黄柏液促进Ⅲ度烧伤患者肉芽组织形成的生物活性成分为槲皮素,潜在通路为PI3K-AKT信号通路,为复方黄柏液治疗Ⅲ度烧伤的研究提供了思路。

含氮杂环卡宾配体的半三明治铱(Ⅲ)配合物的合成及抗肿瘤转移作用

铱(Ⅲ)抗肿瘤配合物[(η^(5)-C_(5)MeC_(6)H_(4)C_(6)H_(5))Ir(C∧C)Cl]PF_(6)能够选择性杀伤、抑制肿瘤细胞,但其内在的作用机制尚不完全明确.采用细胞划痕法、迁移及侵袭等实验考察了金属铱(Ⅲ)配合物对A549细胞的抗转移能力,采用免疫组织化学法和蛋白印迹法分析NEDD9、β-catenin表达水平.研究结果显示:金属铱(Ⅲ)配合物对A549细胞有明显的抗转移作用,可能通过作用于NEDD9降低肺癌细胞的增殖和转移.同时该金属配合物也可通过诱发β-catenin蛋白的表达,增加细胞膜表面粘性,降低A549细胞的转移能力.

妊娠糖尿病患者蛋白C、蛋白S和抗凝血酶Ⅲ水平及其与孕周的关系

目的探讨妊娠糖尿病(GDM)患者蛋白C(PC)、蛋白S(PS)和抗凝血酶Ⅲ(ATⅢ)水平及其与孕周的关系。方法选择2019年1月至2023年3月在医院就诊的232例GDM孕妇作为观察组,同时随机选取同期在医院进行孕检的268例正常孕妇作为对照组,比较两组一般资料和PC、PS和ATⅢ水平;按孕周将观察组和对照组分为孕中期和孕晚期,分别比较孕中期和孕晚期观察组和对照组PC、PS和ATⅢ水平。通过相关性分析,分析PC、PS和ATⅢ水平与孕周的相关性。结果与对照组相比,观察组PS和PC水平降低(P<0.05),但ATⅢ水平差异无统计学意义(P>0.05)。GDM患者PC和ATⅢ水平与孕周呈负相关(r=-0.156、-0.134,P<0.05)。结论GDM患者PS和PC水平降低,且随孕周增大,PC、PS和ATⅢ水平降低,应尽早进行干预,以减少血栓形成的风险。

OPD-ScanⅢ及IOL-Master 700测量白内障术前角膜曲率和水平角膜直径的比较

目的比较OPD-ScanⅢ与IOL-Master 700在角膜曲率(平坦曲率K1与陡峭曲率K2)及水平角膜直径(WTW)测量上的差异性及一致性。方法选取2021年10月至2022年9月在航天中心医院眼科行白内障手术的患者268例328眼(右眼164例,左眼164例)。采用OPD-ScanⅢ及IOL-Master 700仪器分别检测患者左右眼的K1、K2以及WTW值并进行分析比较。采用配对t检验进行参数比较;Pearson相关分析检测参数间的相关性;Bland-Altman以及组内相关系数(ICC)分析确定两种仪器相关参数测量的一致性。结果在左右眼参数的测量中,OPD-ScanⅢ测得的K1及K2值均大于IOL-Master 700,WTW值均低于IOL-Master 700的测量值,差异均有统计学意义(均为P<0.001)。OPD-ScanⅢ在左右眼中测得的K1、K2及WTW值与IOL-Master 700所测得的对应值均呈正相关,差异均有统计学意义(均为P<0.001)。两种仪器K1、K2及WTW值在95%一致性界限外的比例均在5%以内,但95%界限内差值绝对值均接近或超过了1.0 D,存在较大的临床偏差。ICC分析证实两种仪器左右眼测量K1及K2值一致性均极好(ICC>0.90)。两种仪器测得的WTW差值与K2值均显著相关(均为P<0.05)。WTW正偏差范围外有5例(83.33%)样本IOL-Master 700测得的K 2在47.03以上。结论OPD-ScanⅢ及IOL-Master 700在测量K1、K2及WTW上存在偏差,临床应用中不可互相替代。IOL-Master 700测得的WTW值大于OPD-ScanⅢ,当其测量的K2≥47.03时,所获得的WTW值参考价值可能有限。