Early Screening of Rice Varieties with High Essential Amino Acids by Aseptic Seedling Method

With 5 rice varieties( Yifeng 3321,Yifeng 3315,Yifeng 3317,Yifeng 45,Yifeng 14-24) as the experimental objects,the aseptic seedling method was used to culture the rice seeds,and the HPLC was used to determine the content of amino acids in aseptic rice seedlings as well as the total content of 8 kinds of essential amino acids and the content of protein. And the content of protein and amino acids in the polished rice of 5 varieties was detected,and the analysis of variance was performed for screening of the varieties containing high essential amino acids. The rice with high essential amino acids could be produced to meet people's rice quality requirements. The analysis of variance indicated that there were significant differences( F = 39. 262,p = 0. 01) in the content of essential amino acids among 5 kinds of rice varieties. By the Duncan's multiple comparison,the differences among rice varieties were calculated to obtain screening results. The aseptic seedlings:Yifeng 3321(7. 268),Yifeng 3315(6. 395),Yifeng 3317(5. 698),Yifeng 45(5. 567),Yifeng 14-24(5. 190). Rice: Yifeng 3321(2.998),Yifeng 14-24(2. 627),Yifeng 3317(2. 611),Yifeng 3315(2. 503),Yifeng 45(2. 281). Based on the estimated human daily requirement of essential amino acids and amino acids requirement pattern,it was found that the polished rice of 5 varieties was up to standard.The variety with the highest content of essential amino acids was Yifeng 3317(3830 mg/100g),followed by Yifeng 3321(3770 mg/100 g).The results showed that the high quality rice variety in this experiment was Yifeng 3321.

Effects of Exogenous Hormones on Subculture Multiplication and Root Induction of Tissuecultured Seedlings of Mulberry

Objective The purpose of this experiment was to investigate the effects of exogenous hormone factors on subculture multiplication and root induction of tis-suecultured seedlings in mulberry. [Method] The aseptic seedlings of Guisangyou 12 were used as the materials and the comparative experiment on subculture multi-plication and root induction used different kinds,concentrations and combinations of exogenous hormones. [Result] The hormone combination of 0.1 mg/L IBA＋1.5 mg/L 6-BA＋0.03 mg/L TDZ showed the best effects to bud multiplication, and the multiple of bud multiplication was 5.28. The effects of NAA was better than IBA and IAA on root induction. The hormone combination of 2.0 mg/L NAA＋1.0 mg/L PP333 was most suitable to the root induction, in which the rooting rate arrived to 100%, the root number was 7.01 and the root length was 1.38 cm on average. [Conclusion] The results wil provide some technical reference for large-scale propagation of mul-berry seedlings in vitro.

Study on Major Factors Influencing the Proliferation and Growth of Aseptic Buds of Vietnam Mesona blumes

[Objective]This study aimed to investigate the major factors influencing the proliferation and growth of aseptic buds of Vietnam Mesona blumes,thus providing technical references for establishing rapid propagation system in vitro of Vietnam M.blumes.[Method]The effects of medium components（different basic medium,sucrose concentrations and cytokinins）and explant materials（different explants）on the proliferation of aseptic buds of Vietnam M.blumes were analyzed.[Result]Among MS,Miller and modified White basic medium,MS medium exhibited the best effect on proliferation of Vietnam M.blumes buds with the bud proliferation multiple of 8.53.Among different sucrose concentrations,20.0 g/L sucrose gave the highest bud proliferation multiple of 8.34,while 25.0 g/L sucrose led to the best growth status with the bud proliferation multiple of 7.05.The effect of CPPU on bud proliferation was higher than that of 6-BA and KT,with the bud proliferation multiple of 9.80.Among different explants,top stem exhibited the best effect on bud proliferation with the bud proliferation multiple of 9.35,resulting in robust seedlings.[Conclusion]The most suitable subculture medium for the proliferation and growth of aseptic buds of Vietnam M.blumes is MS＋20.0-25.0 g/L sucrose＋0.5 mg/L CPPU and the most suitable explant is top stem.

Preliminary Studies on the Tissue Culture of Cannabis sativa L.(Industrial hemp)

The internodes of the new cultivar Long-ma No.1 of Cannabis sativa L.（Industrial hemp） were used as explants for tissue culture. The paper studied the key factors of industrial hemp tissue culture, such as the physiological state of aseptic seedlings, the selection and concentration of plant growth regulators and so on.Hemp seed disinfection used 75% alcohol for 2 min and sterilized in 1‰ Hg Cl2 for 5min. The best combinations of plant growth regulators were 1.0 mg/L 6-BA and 0.5mg/L NAA for the induction of callus, and the best combinations of hormones were1.0 mg/L KT and 0.5 mg/L NAA for differentiation rate of adventitious bud.