DNA polymorphisms from nucleotide insertion/deletions(InDels)in genomic sequences are the basis for developing InDel molecular markers.To validate the InDel primer pairs on the basis of the comparative genomic study o...DNA polymorphisms from nucleotide insertion/deletions(InDels)in genomic sequences are the basis for developing InDel molecular markers.To validate the InDel primer pairs on the basis of the comparative genomic study on DNA sequences between an Indica rice 93-11 and a Japonica rice Nipponbare for identifying Indica and Japonica rice varieties and studying wild Oryza species,we studied 49 Indica,43 Japonica,and 24 wild rice accessions collected from ten Asian countries using 45 InDel primer pairs.Results indicated that of the 45 InDel primer pairs,41 can accurately identify Indica and Japonica rice varieties with a reliability of over 80%.The scatter plotting data of the principal compo-nent analysis(PCA)indicated that:(i)the InDel primer pairs can easily distinguish Indica from Japonica rice varieties,in addition to revealing their genetic differentiation;(ii)the AA-genome wild rice species showed a relatively close genetic relationship with the Indica rice varieties;and(iii)the non-AA genome wild rice species did not show evident differentiation into the Indica and Japonica types.It is con-cluded from the study that most of the InDel primer pairs obtained from DNA sequences of 93-11 and Nipponbare can be used for identifying Indica and Japonica rice varieties,and for studying genetic relationships of wild rice species,particularly in terms of the Indica-Japonica differentiation.展开更多
基金This study was supported by the Italian Ministry of Environment and Environmental Protection,within the research project Application of biotechnology to the protection of the environment in collaboration with China.
文摘DNA polymorphisms from nucleotide insertion/deletions(InDels)in genomic sequences are the basis for developing InDel molecular markers.To validate the InDel primer pairs on the basis of the comparative genomic study on DNA sequences between an Indica rice 93-11 and a Japonica rice Nipponbare for identifying Indica and Japonica rice varieties and studying wild Oryza species,we studied 49 Indica,43 Japonica,and 24 wild rice accessions collected from ten Asian countries using 45 InDel primer pairs.Results indicated that of the 45 InDel primer pairs,41 can accurately identify Indica and Japonica rice varieties with a reliability of over 80%.The scatter plotting data of the principal compo-nent analysis(PCA)indicated that:(i)the InDel primer pairs can easily distinguish Indica from Japonica rice varieties,in addition to revealing their genetic differentiation;(ii)the AA-genome wild rice species showed a relatively close genetic relationship with the Indica rice varieties;and(iii)the non-AA genome wild rice species did not show evident differentiation into the Indica and Japonica types.It is con-cluded from the study that most of the InDel primer pairs obtained from DNA sequences of 93-11 and Nipponbare can be used for identifying Indica and Japonica rice varieties,and for studying genetic relationships of wild rice species,particularly in terms of the Indica-Japonica differentiation.
