Two New Furostanol Glycosides from Asparagus cochinchinensis

Two new furostanol oligoglycosides named as aspacochioside A (1) and B (2) were isolated from the roots of Asparagus cochinchinensis (Lour.) Merr.. Their structures were elucidated to be 3-O-[{a-L-rhamnopyranosyl-(14)}{b-D-glucopyranosyl}]-26-O-[b-D-glucopy- ranosyl]-(25S)-5b-furostane-3b,22a,26-triol 1 and 3-O-[{a-L-rhamnopyranosyl-(14)}{b-D-glu- copyranosyl}]-26-O-[b-D-glucopyranosyl]-22a-methoxy-(25S)-5b-furostane-3b,26-diol 2 on the basis of spectroscopic techniques and chemical methods.

Furostanol Saponins from Asparagus cochinchinensis and Their Cytotoxicity

Phytochemical investigation on the roots of Asparagus cochinchinensis led to the isolation of one new furostanol saponin,named 26-O-β-d-glucopyranosyl-22α-hydroxyl-(25R)-Δ5(6)-furost-3β,26-diol-3-O-α-l-rhamnopyranosyl-(1→2)-[β-d-glucopyranosyl-(1→4)-α-l-rhamnopyranosyl-(1→4)]-β-d-glucopyranoside(1),along with three known congeners(2‒4).The structure of new saponin was elucidated via comprehensive inspection of its HRMS and NMR spectral data as well as chemical technology,whereas those of known ones were identified by comparison of their NMR and MS spectral data with those reported in literatures.All isolated saponins were evaluated for their cytotoxic effects on two human liver(MHCC97H)and lung adenocarcinoma(H1299)cancer cells in vitro.Among them,both 1 and 2 showed significant cytotoxicity against above mentioned cell lines.Further studies revealed that these two saponins could significantly inhibit their proliferation of MHCC97H and H1299 cells.

A New Furost-20(22)-ene Oligoglycoside from Asparagus cochinchinensis

A new furost-20(22)-ene oligoglycoside named as aspacochioside C was isolated from the roots of Asparagus cochinchinensis (Lour.) Merr.. Its structure was elucidated to be 26-O-b- D-glucopyranosyl-(25S)-5b-furost-20(22)-en-3b,26-diol-3-O-a-L-rhamnopyranosyl-(14)-b-D- glucopyranoside on the basis of spectroscopic techniques including 1D and 2D NMR experiments.

Two new furostanol glycosides fromAsparagus cochinchinensis(Lour.) Merr.

Two new furostanol glycosides, aspacochinosides L （1） and M （2）, were isolated from the roots of Asparagus cochinchinensis （Lour.） Merr. Their structures were determined by chemical methods and NMR spectral analysis, including extensive 1D and 2D NMR experiments. Compounds 1 and 2 were investigated for their anti-neuroinflammatory activity in LPS-induced BV-2 cells. Compound 2 showed moderate inhibitory effect on NO production in LPS-induced BV-2 microglial cells with IC 50 value of 32.26 μM.

Steroidal saponins from the roots of Asparagus cochinchinensis

AIM： To study the chemical constituents of the roots of Asparagus cochinchinensis （Asparagaceae）. METHOD： The compounds were isolated with Diaion HP20, silica gel, and ODS chromatography, and their structures were de- termined on the basis of chemical methods, HR-ESI-MS, and 1 D- and 2D-NMR techniques. RESULTS： Seven compounds were isolated from the n-butanol fraction of the roots of A. cochinchinensis, and their structures were elucidated as （25S）-26-O-β-D-glucopyranosyl-5β-furostan-3β, 22a, 26-triol-12-one-3-O-β-D-glucopyranoside （1）, （25S）-26- O-β-D-glucopyranosyl-22a-methoxy-5β-furostan-3p, 26-diol-12-one-3-O-β-D-glucopyranoside （2）, （25S）-26-O-β-D-glucopyra- nosyl-5β-furostan-3β, 22a, 26-triol （3）, （25S）-26-O-β-D-glucopyranosyl-5β-furstan-3β, 22a, 26-triol-3-O-β-D-glucopyranoside （4）, （25S）-26-O-β-D-glucopyranosyl-5β-furostan-3β, 22a, 26-triol-3-O-a-L-rhamnopyranosyl-（1, 4）-β-D-glucopyranoside （5）, （25S）- 5β-spirostan-3fl-ol-3-O-a-L-rhamnopyranoside （6）, and （25S）-5β-spirostan-3β-ol-3-O-β-D-glucopyranoside （7）. CONCLUSION： Compounds 1 and 2 were two new furostanol saponins.

外源硒对天门冬的生长及有效成分含量的影响

在灌根法施加外源硒的条件下,研究天门冬[Asparagus cochinchinensis(Lour.)Merr]生长(茎长、块根数量、块根长度、块根直径、块根干重)以及天门冬块根有效成分含量的变化规律。结果表明,种植天门冬时向土壤中施加10~40 mg/L的外源硒有利于天门冬的生长。当外源硒浓度为30 mg/L时,每株天门冬块根数量最多,当外源硒浓度为50 mg/L时,对天门冬块根数量有抑制作用;当外源硒浓度为40 mg/L时,天门冬茎长、块根长度、块根直径及块根干重最佳;当外源硒浓度为10~50 mg/L时,对有效成分的积累表现为促进作用,天门冬多糖、皂苷与黄酮积累的整体最适宜外源硒浓度为30 mg/L。