CMC固定化烟曲霉活菌体小球中微生物的增殖及基质消耗动力学

对采用基础培养基分批培养过程中羧甲基纤维素钠(Carboxymethyl cellulose sodium,CMC)固定化烟曲霉(Aspergillus fumigatus)活菌体小球中菌体的生长动力学和基质消耗动力学进行了考察,结果表明,Logistic方程能很好地反映CMC固定化烟曲霉活菌体小球中的菌体生长动力学,CMC固定化烟曲霉活菌体小球中的菌体生长未受到明显抑制;基质消耗动力学方程可以根据产物合成模型Luedeking-Piret方程得到。

Purification and characterization of extracellular chitinase from a novel strain Aspergillus fumigatus CS-01

Twelve samples derived from different locations in south central area of China are treated by enrichment and spread-plate technique for initial screening. Seven chitinase-producing strains are isolated. The chitinase present in the culture supematant of strain CS-01 possesses the maximum activity of 0.118 U/mL. Analysis of the morphological feature and the ITS rDNA sequence reveals that strain CS-01 belongs to Aspergillus fumigatus. Production of the chitinase is regulated by a inducible way and the maximum activity appears at 36 h in colloidal chitin culture. Purification of the chitinase is carried out by salting out, gel filtrate chromatography and anion exchange chromatography sequentially. Native-PAGE and SDS-PAGE indicate that the chitinase from A. fumigatus CS-01 is a monomer with the relative molecular mass estimated to be 4.50 × 10^4. Its maximum activity appears at pH 5 and 55 ℃. The chitinase is stable at pH 4.0-7.5 and below 45 ℃.

海洋真菌烟曲霉Aspergillus fumigatus YK-7中生物碱类代谢产物及其抗肿瘤活性研究

目的研究海洋真菌烟曲霉Aspergillus fumigatus YK-7中的生物碱类代谢产物。方法利用多种色谱方法(硅胶柱色谱、ODS柱色谱、Sephadex LH-20柱色谱、高效液相色谱)进行分离纯化,通过理化性质和波谱数据鉴定化合物的结构。采用台盼蓝法和MTT法进行抗肿瘤活性测试。结果从发酵液和菌丝体中分离得到10个生物碱类化合物,分别鉴定为pseurotin A(1)、pseurotin A1(2)、14-norpseurotin A(3)、FD-838(4)、demethoxyfumitremorgin C(5)、9β-hydroxyverruculogen TR-2(6)、6-methoxyspirotryprostatin B(7)、spiro[5H,10H-dipyrrolo[1,2-a:1',2'-d]pyrazine-2-(3H),2'-[2H]indole]-3',5,10(1'H)-trione(8)、terezine D(9)和14-hydroxyterezine D(10)。结论化合物3、6、7、9、10为首次从海洋来源烟曲霉中分离得到。化合物1~4具有一定的抗肿瘤活性。