Oscillating field stimulation(OFS)is a potential method for treating spinal cord injury.Although it has been used in spinal cord injury(SCI)therapy in basic and clinical studies,its underlying mechanism and the correl...Oscillating field stimulation(OFS)is a potential method for treating spinal cord injury.Although it has been used in spinal cord injury(SCI)therapy in basic and clinical studies,its underlying mechanism and the correlation between its duration and nerve injury repair remain poorly understood.In this study,we established rat models of spinal cord contusion at T10 and then administered 12 weeks of OFS.The results revealed that effectively promotes the recovery of motor function required continuous OFS for more than 6 weeks.The underlying mechanism may be related to the effects of OFS on promoting axon regeneration,inhibiting astrocyte proliferation,and improving the linear arrangement of astrocytes.This study was approved by the Animal Experiments and Experimental Animal Welfare Committee of Capital Medical University(supplemental approval No.AEEI-2021-204)on July 26,2021.展开更多
Transplantation of somatic cells,including bone marrow stromal cells（BMSCs）,bone marrow mononuclear cells（BMNCs）,and choroid plexus epithelial cells（CPECs）,enhances the outgrowth of regenerating axons and promot...Transplantation of somatic cells,including bone marrow stromal cells（BMSCs）,bone marrow mononuclear cells（BMNCs）,and choroid plexus epithelial cells（CPECs）,enhances the outgrowth of regenerating axons and promotes locomotor improvements.They are not integrated into the host spinal cord,but disappear within 2-3 weeks after transplantation.Regenerating axons extend at the spinal cord lesion through the astrocyte-devoid area that is filled with connective tissue matrices.Regenerating axons have characteristics of peripheral nerves：they are associated with Schwann cells,and embedded in connective tissue matrices.It has been suggested that neurotrophic factors secreted from BMSCs and CPECs promote “intrinsic” ability of the spinal cord to regenerate.Transplanted Schwann cells survive long-term,and are integrated into the host spinal cord,serving as an effective scaffold for the outgrowth of regenerating axons in the spinal cord.The disadvantage that axons are blocked to extend through the glial scar at the border of the lesion is overcome.Schwann cells have been approved for clinical applications.Neural stem/progenitor cells（NSPCs） survive long-term,proliferate,and differentiate into glial cells and/or neurons after transplantation.No method is available at present to manipulate and control the behaviors of NPSCs to allow them to appropriately integrate into the host spinal cord.NPSP transplantation is not necessarily effective for locomotor improvement.展开更多
基金the National Natural Science Foundation of China,No.30801222(to JZB).
文摘Oscillating field stimulation(OFS)is a potential method for treating spinal cord injury.Although it has been used in spinal cord injury(SCI)therapy in basic and clinical studies,its underlying mechanism and the correlation between its duration and nerve injury repair remain poorly understood.In this study,we established rat models of spinal cord contusion at T10 and then administered 12 weeks of OFS.The results revealed that effectively promotes the recovery of motor function required continuous OFS for more than 6 weeks.The underlying mechanism may be related to the effects of OFS on promoting axon regeneration,inhibiting astrocyte proliferation,and improving the linear arrangement of astrocytes.This study was approved by the Animal Experiments and Experimental Animal Welfare Committee of Capital Medical University(supplemental approval No.AEEI-2021-204)on July 26,2021.
基金supported by grants from the Japanese Ministry of Education,Culture,Sports,Science and Technology(No.2300125 to CI and No.26870744 to KK)
文摘Transplantation of somatic cells,including bone marrow stromal cells（BMSCs）,bone marrow mononuclear cells（BMNCs）,and choroid plexus epithelial cells（CPECs）,enhances the outgrowth of regenerating axons and promotes locomotor improvements.They are not integrated into the host spinal cord,but disappear within 2-3 weeks after transplantation.Regenerating axons extend at the spinal cord lesion through the astrocyte-devoid area that is filled with connective tissue matrices.Regenerating axons have characteristics of peripheral nerves：they are associated with Schwann cells,and embedded in connective tissue matrices.It has been suggested that neurotrophic factors secreted from BMSCs and CPECs promote “intrinsic” ability of the spinal cord to regenerate.Transplanted Schwann cells survive long-term,and are integrated into the host spinal cord,serving as an effective scaffold for the outgrowth of regenerating axons in the spinal cord.The disadvantage that axons are blocked to extend through the glial scar at the border of the lesion is overcome.Schwann cells have been approved for clinical applications.Neural stem/progenitor cells（NSPCs） survive long-term,proliferate,and differentiate into glial cells and/or neurons after transplantation.No method is available at present to manipulate and control the behaviors of NPSCs to allow them to appropriately integrate into the host spinal cord.NPSP transplantation is not necessarily effective for locomotor improvement.
