Effects of Different Initial Processing Methods on Atractylodin Content of Atractylodes chinensis

[Objectives]The paper was to compare the effects of different initial processing methods on atractylodin content of Atractylodes chinensis.[Methods]The atractylodin content of A.chinensis obtained by different initial processing methods was determined by HPLC.The loss rate on drying was determined by weighing.[Results]In the study of drying methods,the atractylodin content varied significantly among different thicknesses of slices,and the overall content of the product with the slice thickness of 5 mm was higher.Among different drying methods,constant temperature vacuum drying and shade drying of 5 mm slice resulted in the highest content of atractylodin.In the experiment of root impacting,root impacting twice received the best effect,and there was no significant difference in the atractylosin content of A.chinensis between drying in the sun and drying in the shade after root impacting twice(P>0.05).[Conclusions]Constant temperature vacuum drying or drying in the shade with the slice thickness of 5 mm,and root impacting twice is the best initial processing method,which leads to high atractylodin content of A.chinensis and good quality of medicinal materials.

Quality Evaluation of Atractylodes chinensis(DC.)Koidz.in Chengde Region Based on Dual Component Analysis

[Objectives]To evaluate the quality of Atractylodes chinensis(DC.)Koidz.in Chengde region.[Methods]35 samples of A.chinensis from different growth years were collected,and a high performance liquid chromatography(HPLC)method was established for the dual components of atractylodin and atractylone,and the quality evaluation was conducted on the cultivated A.chinensis from Chengde region in terms of moisture,ash and volatile oil content.[Results]The moisture content of all samples met the regulations,and the content of volatile oil was relatively high.Among them,seven samples with atractylodin content lower than 0.3%were unqualified products(accounting for 20%),and one sample had excessive ash content(accounting for 3%).[Conclusions]The established method for the determination of the dual components of Atractylodis Rhizoma is stable,simple,fast,precise and reusable.Besides,according to the Pearson correlation analysis among the indicators,it is concluded that the atractylodin and atractylone content are significantly related to the growth period and the volatile oil content.In addition,the quality evaluation indicators of A.chinensis in Chengde region show that there are differences in the quality of Atractylodis Rhizoma,but the overall pass rate is relatively high.It is necessary to further study the reasons for the differences.

Effects of plug tray cell size on the growth of Atractylodes Chinensis (DC) Koidz seedlings

Atractylodes Chinensis(DC)Koidz is a perennial herb often used as a prescription medicine for influenza,to invigorate the spleen and remove dampness.The quality of the herb is determined by the quality of the seedlings.The traditional A.chinensis seedling production technique has nonuniform seedlings and low mechanisation.The plug seedling technique was used to produce A.Chinensis seedlings.This study was conducted to determine the growth characteristics of A.Chinensis seedlings according to the cell size of plug trays and number of days after sowing.Plant height,stem diameter,rhizome diameter,number of leaves,leaf area,and shoot dry and fresh weight of A.Chinensis seedlings were significantly higher in the P32-D treatment than in the P72,P50,and P32 treatments,but not significantly different from the P28-D treatment 60 d after sowing.The P28-D treatment resulted in a considerable drop in rhizome fresh weight and healthy seedling index,both of which are key indicators for assessing seedling quality.Although the differences in Pn among treatments were not significantly different,Tr was considerably greater in A.Chinensis seedlings treated with P32-D and P28-D than in the other three treatments.The P32-D and P28-D treatments had considerably greater potential maximum photochemical efficiency of PSII,quantum yield of photosystem II,photochemical quenching and electron transport rate than the other three treatments.Root vitality of A.Chinensis seedlings was significantly stronger in the P32-D treatment than in the other four treatments,and it was 1.9 times higher than in the P28-D treatment.The soluble protein,soluble sugar,and starch contents of A.Chinensis seedlings were highest in the P32-D treatment,but the differences among treatments were not significant.LUE,EUE,PY and EY were significantly higher in the P32-D treatment than in the other four treatments,being 1.3,1.3,1.4 and 1.4 times higher than in P28-D,respectively.On the other hand,as for the change in the growth of A.Chinensis seedlings according to the number of days after sowing,the growth of shoots and rhizome was most vigorous at the 60 d after sowing,while the fresh weight of the rhizome,which can be considered as an indicator of root growth,increased steadily during the experiment but slowed down after 60 d.As a result,32-cell deepened plug trays and a seedling nursery for a period of 60-75 d are recommended for commercial cultivation of A.Chinensis seedlings.This will provide technical support for production in A.Chinensis seedling.

(±)-Atrachinenins A-C,Three Pairs of Caged C_(27) Meroterpenoids from the Rhizomes of Atractylodes chinensis

Comprehensive Summary(±)-Atrachinenins A—C(1—3),three meroterpenoids possessing novel C_(27) skeletons,were isolated from the rhizomes of Atractylodes chinensis.(±)-Atrachinenin A(1)possesses an unprecedented cage-like skeleton with a fused 6/6/5/5 tetracyclic system(decahy-dro-1,5:4a,7-dimethanobenzo[7]annulene),which may be formed by the intermolecular cyclization of(±)-atrachinenin C(3).