Manganese phthalocyanine (MnPc)-Fe3O4 nanoparticles composite was prepared and characterized. The results show that MnPc are completed on the surface of Fe3O4 nanoparticles in the state of single molecule. There is ef...Manganese phthalocyanine (MnPc)-Fe3O4 nanoparticles composite was prepared and characterized. The results show that MnPc are completed on the surface of Fe3O4 nanoparticles in the state of single molecule. There is effective composite between MnPc and Fe3O4 nanoparticles which can improve the antioxidization ability of Fe3O4 nanoparticles greatly. The composite was dispersed into chlorinated paraffin oil to form electromag-netorheological (EMR) fluid with high activity, and the EMR properties of this EMR fluid are studied.展开更多
An aptamer-based colorimetric biosensor was developed, which could be used to detect residual strepto- mycin from food quickly and cost-effectively. The ssDNA aptamer target for streptomycin was obtained using sys- te...An aptamer-based colorimetric biosensor was developed, which could be used to detect residual strepto- mycin from food quickly and cost-effectively. The ssDNA aptamer target for streptomycin was obtained using sys- temic evolution of ligands by exponential enrichment through affinity chromatography. A total of 19 candidates were obtained after 10 rounds of selection and were grouped into 3 families according to their similarity of sequence and structure. Among them, aptamer A15 showed the highest affinity for streptomycin tested by fluorescence intensity analysis with a dissociation constant of 6.07 nmol/L. Aptamer A15 also exhibited a higher streptomycin specificity with the lowest detectable limit of 25 nmol/L according to the value of A620/A520 ratio with AuNP-based colorimetric assay. The method was specific and sensitive for the detection of streptomycin from simply-treated milk and honey at 100 and 125 nmol/L, respectively, and is a promising approach to monitor antibiotics or other small molecules ana- logues in food inspection.展开更多
基金Funded by Natural Sciene Fund of China (No. 29674021).
文摘Manganese phthalocyanine (MnPc)-Fe3O4 nanoparticles composite was prepared and characterized. The results show that MnPc are completed on the surface of Fe3O4 nanoparticles in the state of single molecule. There is effective composite between MnPc and Fe3O4 nanoparticles which can improve the antioxidization ability of Fe3O4 nanoparticles greatly. The composite was dispersed into chlorinated paraffin oil to form electromag-netorheological (EMR) fluid with high activity, and the EMR properties of this EMR fluid are studied.
基金Supported by the National Natural Science Foundation of China（No.81202338）, the Natural Science Foundation of Hebei Province, China（No.H2016201121）, the National Training Program of Innovation and Entrepreneurship for Undergraduates, China（No.201610075001）, the Postdoctoral Science Foundation of China（No.2013M530885） and the Science and Technology Research Project of Hebei Higher Education Institutions, China（No. ZD2017010）.
文摘An aptamer-based colorimetric biosensor was developed, which could be used to detect residual strepto- mycin from food quickly and cost-effectively. The ssDNA aptamer target for streptomycin was obtained using sys- temic evolution of ligands by exponential enrichment through affinity chromatography. A total of 19 candidates were obtained after 10 rounds of selection and were grouped into 3 families according to their similarity of sequence and structure. Among them, aptamer A15 showed the highest affinity for streptomycin tested by fluorescence intensity analysis with a dissociation constant of 6.07 nmol/L. Aptamer A15 also exhibited a higher streptomycin specificity with the lowest detectable limit of 25 nmol/L according to the value of A620/A520 ratio with AuNP-based colorimetric assay. The method was specific and sensitive for the detection of streptomycin from simply-treated milk and honey at 100 and 125 nmol/L, respectively, and is a promising approach to monitor antibiotics or other small molecules ana- logues in food inspection.
