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J61321(Alteromonas aurantia)菌株产铁载体培养条件的研究 被引量:5
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作者 李俊峰 李红芳 +1 位作者 姚淑敏 池振明 《青岛科技大学学报(自然科学版)》 CAS 2007年第1期20-24,共5页
在摇瓶水平上采用单次单因子方法对铁载体高产菌株J61321(Alteromonas au- rantia)的生长和产铁载体的发酵条件进行优化,考察了碳源、氮源、培养时间、培养温度、初始pH值、摇床转速等因素的影响,获得菌株J61321产铁载体的最佳发酵条件... 在摇瓶水平上采用单次单因子方法对铁载体高产菌株J61321(Alteromonas au- rantia)的生长和产铁载体的发酵条件进行优化,考察了碳源、氮源、培养时间、培养温度、初始pH值、摇床转速等因素的影响,获得菌株J61321产铁载体的最佳发酵条件:酵母粉0.1%(W/V),蛋白胨0.2%(W/V),FePO_40.01g·L^(-1),温度28℃,转速180 r·min^(-1), pH7.5,铁载体在菌株J61321培养22 h后达到最大产量。在最适培养条件下测定了出发菌株A18和突变株J61321不同培养时间的菌液OD_(600nm)值、铁载体产量和抑菌活性,结果显示二者的生长量差距不大(p>0.05),但是铁载体产量和抑菌活性之间存在显著性差异(p<0.05)。 展开更多
关键词 Alteromonas aurantia 单次单因子 显著性差异 铁载体
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Characterization of a Mutant of Alteromonas aurantia A18 and Its Application in Mariculture 被引量:4
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作者 LI Junfeng CHI Zhenming +1 位作者 LI Hongfang WANG Xianghong 《Journal of Ocean University of China》 SCIE CAS 2008年第1期55-59,共5页
Mutant J61321 with enhanced siderophore production of Alteromonas aurantia AI8 was obtained after a series of chemical-physical mutageneses. It was found that the antibacterial activity against Vibrio anguillarum W-1 ... Mutant J61321 with enhanced siderophore production of Alteromonas aurantia AI8 was obtained after a series of chemical-physical mutageneses. It was found that the antibacterial activity against Vibrio anguillarum W-1 and siderophore production of the mutant were higher than those by the original strain A 18 which had been used in mariculture. The results of the specific assay(Csaky and Arnow methods) of siderophore showed that the sidrophore with hydroxamate group was produced by mutant J61321 and the original strain A 18, respectively, while the siderophore with catechol group was yielded by strain W-1 (Aibrio anguillarum). Meanwhile, the siderophore yield, antibacterial activity and anti-chelator activity of strain J61321 were higher than those of its parent strain A 18. 展开更多
关键词 Alteromonas aurantia A18 PROBIOTICS SIDEROPHORE MUTAGENESIS
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