A new reaction system to determine nonlinear chemical fingerprint(NCF)and its use in identification method based on double reaction system was researched.Panax ginsengs,such as ginseng,American ginseng and notoginseng...A new reaction system to determine nonlinear chemical fingerprint(NCF)and its use in identification method based on double reaction system was researched.Panax ginsengs,such as ginseng,American ginseng and notoginseng were identified by the method.The NCFs of the three samples of Panax ginsengs were determined through two nonlinear chemical systems,namely system 1 consisting of sample components,H2SO4,MnSO4,NaBrO3,acetone and the new system,system 2 consisting of sample components,H2SO4,(NH4)4Ce(SO4)2,NaBrO3 and citric acid.The comparison between the results determined through systems 1 and 2 shows that the speed to determine NCF through system 2 is much faster than that through system 1;for systems 1 and 2,the system similarities of the same kind of samples are≥98.09%and 99.78%,respectively,while those of different kinds of samples are≤63.04%and 86.34%,respectively.The results to identify the kinds of some samples by system similarity pattern show that both the accuracies of identification methods based on single system 1 and 2 are≥95.6%,and the average values are 97.1%and 96.3%,respectively;the accuracy of the method based on double system is≥97.8%,and the average accuracy is 99.3%.The accuracy of the method based on double system is higher than that based on any single system.展开更多
In this study,we established a DNA extraction method of meats and a PCR method for the detection of mink-derived compo-nents in common edible meats.The DNA of meats was extracted by a developed mink-derived component ...In this study,we established a DNA extraction method of meats and a PCR method for the detection of mink-derived compo-nents in common edible meats.The DNA of meats was extracted by a developed mink-derived component detection method and salting-out method,the specific primers of Cyt-b were designed,and the mink-derived components in common edible meats were identified by PCR.The results showed that the concentration and purity of mink meat DNA extracted by the two methods could meet the requirements of PCR.The mink meat DNA extracted by the developed mink DNA detection method had a high purity and yield.The mink-derived DNA in common edible meats could be identified.The specificity of mink meat DNA primer was proved to be good by repeated tests.The developed mink DNA detection method has the advantages of rapid detection and simple operation,which can be used for the identification of meat products in market and provide an experimental basis for the quality control of meat products.展开更多
基金Project(61533021)supported by the National Natural Science Foundation of ChinaProject(R201706)supported by Hunan Food Pharmaceutical,China
文摘A new reaction system to determine nonlinear chemical fingerprint(NCF)and its use in identification method based on double reaction system was researched.Panax ginsengs,such as ginseng,American ginseng and notoginseng were identified by the method.The NCFs of the three samples of Panax ginsengs were determined through two nonlinear chemical systems,namely system 1 consisting of sample components,H2SO4,MnSO4,NaBrO3,acetone and the new system,system 2 consisting of sample components,H2SO4,(NH4)4Ce(SO4)2,NaBrO3 and citric acid.The comparison between the results determined through systems 1 and 2 shows that the speed to determine NCF through system 2 is much faster than that through system 1;for systems 1 and 2,the system similarities of the same kind of samples are≥98.09%and 99.78%,respectively,while those of different kinds of samples are≤63.04%and 86.34%,respectively.The results to identify the kinds of some samples by system similarity pattern show that both the accuracies of identification methods based on single system 1 and 2 are≥95.6%,and the average values are 97.1%and 96.3%,respectively;the accuracy of the method based on double system is≥97.8%,and the average accuracy is 99.3%.The accuracy of the method based on double system is higher than that based on any single system.
基金financially supported by the Science and Technology Development Program of Jilin Province (20160204004NY, 20200404152YY, 20200403047SF)the Science and Technology Innovation Center Construction Program of Jilin Province (20190902018TC)the Graduate Innovation Program of Beihua University [Beihua Yanchuanghezi 2019 (016)]
文摘In this study,we established a DNA extraction method of meats and a PCR method for the detection of mink-derived compo-nents in common edible meats.The DNA of meats was extracted by a developed mink-derived component detection method and salting-out method,the specific primers of Cyt-b were designed,and the mink-derived components in common edible meats were identified by PCR.The results showed that the concentration and purity of mink meat DNA extracted by the two methods could meet the requirements of PCR.The mink meat DNA extracted by the developed mink DNA detection method had a high purity and yield.The mink-derived DNA in common edible meats could be identified.The specificity of mink meat DNA primer was proved to be good by repeated tests.The developed mink DNA detection method has the advantages of rapid detection and simple operation,which can be used for the identification of meat products in market and provide an experimental basis for the quality control of meat products.
