Auxin is throughout the entire life process of plants and is involved in the crosstalk with other hormones,yet its role in apple disease resistance remains unclear.In this study,we investigated the function of auxin/i...Auxin is throughout the entire life process of plants and is involved in the crosstalk with other hormones,yet its role in apple disease resistance remains unclear.In this study,we investigated the function of auxin/indole-3-acetic acid(IAA)gene Md IAA24 overexpression in enhancing apple resistance to Glomerella leaf spot(GLS)caused by Colletotrichum fructicola(Cf).Analysis revealed that,upon Cf infection,35S::Md IAA24 plants exhibited enhanced superoxide dismutase(SOD)and peroxidase(POD)activity,as well as a greater amount of glutathione(reduced form)and ascorbic acid accumulation,resulting in less H_(2)O_(2)and superoxide anion(O_(2)^(-))in apple leaves.Furthermore,35S::Md IAA24 plants produced more protocatechuic acid,proanthocyanidins B1,proanthocyanidins B2 and chlorogenic acid when infected with Cf.Following Cf infection,35S::Md IAA24 plants presented lower levels of IAA and jasmonic acid(JA),but higher levels of salicylic acid(SA),along with the expression of related genes.The overexpression of Md IAA24 was observed to enhance the activity of chitinase andβ-1,3-glucanase in Cfinfected leaves.The results indicated the ability of Md IAA24 to regulate the crosstalk between IAA,JA and SA,and to improve reactive oxygen species(ROS)scavenging and defense-related enzymes activity.This jointly contributed to GLS resistance in apple.展开更多
Carbon monoxide(CO)and nitric oxide(NO)are signal molecules that enhance plant adaptation to environmental stimuli.Auxin is an essential phytohormone for plant growth and development.CO and NO play crucial roles in mo...Carbon monoxide(CO)and nitric oxide(NO)are signal molecules that enhance plant adaptation to environmental stimuli.Auxin is an essential phytohormone for plant growth and development.CO and NO play crucial roles in modulating the plant’s response to iron deficiency.Iron deficiency leads to an increase in the activity of heme oxygenase(HO)and the subsequent generation of CO.Additionally,it alters the polar subcellular distribution of Pin-Formed 1(PIN1)proteins,resulting in enhanced auxin transport.This alteration,in turn,leads to an increase in NO accumulation.Furthermore,iron deficiency enhances the activity of ferric chelate reductase(FCR),as well as the expression of the Fer-like iron deficiency-induced transcription factor 1(FIT)and the ferric reduction oxidase 2(FRO2)genes in plant roots.Overexpression of the long hypocotyl 1(HY1)gene,which encodes heme oxygenase,or the CO donor treatment resulted in enhanced basipetal auxin transport,higher FCR activity,and the expression of FIT and FRO2 genes under Fe deficiency.Here,a potential mechanism is proposed:CO and NO interact with auxin to address iron deficiency stress.CO alters auxin transport,enhancing its accumulation in roots and up-regulating key iron-related genes like FRO2 and IRT1.Elevated auxin levels affect NO signaling,leading to greater sensitivity in root development.This interplay promotes FCR activity,which is crucial for iron absorption.Together,these molecules enhance iron uptake and root growth,revealing a novel aspect of plant physiology in adapting to environmental stress.展开更多
Subgroup 4(Sg4)members of the R2R3-MYB are generally known as negative regulators of the phenylpropanoid pathway in plants.Our previous research showed that a R2R3-MYB Sg4 member from Camellia sinensis(CsMYB4a)inhibit...Subgroup 4(Sg4)members of the R2R3-MYB are generally known as negative regulators of the phenylpropanoid pathway in plants.Our previous research showed that a R2R3-MYB Sg4 member from Camellia sinensis(CsMYB4a)inhibits expression of some genes in the phenylpropanoid pathway,but its physiological function in the tea plant remained unknown.Here,CsMYB4a was found to be highly expressed in anther and filaments,and participated in regulating filament growth.Transcriptome analysis and exogenous auxin treatment showed that the target of CsMYB4a might be the auxin signal pathway.Auxin/indole-3-acetic acid 4(AUX/IAA4),a repressor in auxin signal transduction,was detected from a yeast two-hybrid screen using CsMYB4a as bait.Gene silencing assays showed that both CsIAA4 and CsMYB4a regulate filament growth.Tobacco plants overexpressing CsIAA4 were insensitive to exogenous a-NAA,consistent with overexpression of CsMYB4a.Protein-protein interaction experiments revealed that CsMYB4a interacts with N-terminal of CsIAA4 to prevent CsIAA4 degradation.Knock out of the endogenous NtIAA4 gene,a CsIAA4 homolog,in tobacco alleviated filament growth inhibition and a-NAA insensitivity in plants overexpressing CsMYB4a.All results strongly suggest that CsMYB4a works synergistically with CsIAA4 and participates in regulation of the auxin pathway in stamen.展开更多
Under appropriate culture conditions,plant cells can regenerate new organs or even whole plants.De novo organ regeneration is an excellent biological system,which usually requires additional growth regulators,includin...Under appropriate culture conditions,plant cells can regenerate new organs or even whole plants.De novo organ regeneration is an excellent biological system,which usually requires additional growth regulators,including auxin and cytokinin.Nitrate is an essential nutrient element for plant vegetative and reproductive development.It has been reported that nitrate is involved in auxin biosynthesis and transport throughout the growth and development of plants.In this study,we demonstrated that the ectopic expression of the MdNLP7 transcription factor in Arabidopsis could regulate the regeneration of root explants.MdNLP7 mainly participated in the regulation of callus formation,starting with pericycle cell division,and mainly affected auxin distribution and accumulation in the regulation process.Moreover,MdNLP7 upregulated the expression of genes related to auxin biosynthesis and transport in the callus formation stage.The results demonstrated that MdNLP7 may play a role in the nitrate-modulated regeneration of root explants.Moreover,the results revealed that nitrate–auxin crosstalk is required for de novo callus initiation and clarified the mechanisms of organogenesis.展开更多
The trichomes of rice leaves are formed by the differentiation and development of epidermal cells.Plant trichomes play an important role in stress resistance and protection against direct ultraviolet irradiation.Howev...The trichomes of rice leaves are formed by the differentiation and development of epidermal cells.Plant trichomes play an important role in stress resistance and protection against direct ultraviolet irradiation.However,the development of rice trichomes remains poorly understood.In this study,we conducted ethylmethane sulfonate(EMS)-mediated mutagenesis on the wild-type(WT)indica rice‘Xida 1B’.Phenotypic analysis led to the screening of a mutant that is defective in trichome development,designated lhl1(less hairy leaf 1).We performed map-based cloning and localized the mutated gene to the 70-kb interval between the molecular markers V-9 and V-10 on chromosome 2.The locus LOC_Os02g25230 was identified as the candidate gene by sequencing.We constructed RNA interference(LHL1-RNAi)and overexpression lines(LHL1-OE)to verity the candidate gene.The leaves of the LHL1-RNAi lines showed the same trichome developmental defects as the lhl1 mutant,whereas the trichome morphology on the leaf surface of the LHL1-OE lines was similar to that of the WT,although the number of trichomes was significantly higher.Quantitative real-time PCR(RT-qPCR)analysis revealed that the expression levels of auxin-related genes and positive regulators of trichome development in the lhl1 mutant were down-regulated compared with the WT.Hormone response analysis revealed that LHL1 expression was affected by auxin.The results indicate that the influence of LHL1 on trichome development in rice leaves may be associated with an auxin pathway.展开更多
Upland cotton(Gossypium hirsutum L.)is the most important natural textile fiber crop worldwide.Plant height(PH)is a significant component of plant architecture,strongly influencing crop cultivation patterns,overall yi...Upland cotton(Gossypium hirsutum L.)is the most important natural textile fiber crop worldwide.Plant height(PH)is a significant component of plant architecture,strongly influencing crop cultivation patterns,overall yield,and economic coefficient.However,cotton genes regulating plant height have not been fully identified.Previously,an HD-Zip gene(GhHB12)was isolated and characterized in cotton,which regulates the abiotic and biotic stress responses and the growth and development processes.In this study,we showed that GhHB12 was induced by auxin.Moreover,overexpression of GhHB12 induces the expression of HY5,ATH1,and HAT4,represses the spatial-temporal distribution,polar transport,and signaling of auxin,alters the expression of genes involved in cell wall expansion,and restrains the plant height in cotton.These results suggest a role of GhHB12 in regulating cotton plant height,which could be achieved by affecting the auxin signaling and cell wall expansion.展开更多
The auxin receptor(TIR1/AFBs)family encodes the F-box protein subunit,which is involved in the formation of the E3 ubiquitin ligase SCFTIR1/AFBs complex,a key component of the auxin signaling pathway.However,there are...The auxin receptor(TIR1/AFBs)family encodes the F-box protein subunit,which is involved in the formation of the E3 ubiquitin ligase SCFTIR1/AFBs complex,a key component of the auxin signaling pathway.However,there are few studies on the auxin receptor family in apple(Malus×domestica).In this study,eight MdAFBs were identified,and phylogenetic analysis showed that they were classified into four groups and distributed on eight chromosomes.Herein,a comprehensive analysis of the MdAFB gene family was conducted to identify cis-acting elements,gene structures,protein structures,aligned sequences,conserved motifs,conserved amino acids,and the protein–protein interaction network.The results of yeast two-hybrid assays showed that MdAFB1 interacted with three auxin repressor proteins.The results of qRT-PCR showed that MdAFB1 responded to osmotic and salt stress.The overexpression of MdAFB1 increased osmotic and salt resistance in apple calli,and the ectopic expression of MdAFB1 enhanced osmotic and salt tolerance in Arabidopsis.This study provided a basis for the identification of auxin receptor genes in apple and their functions in mediating osmotic and salt stress.展开更多
As the reproductive organ of the endangered species Fokienia hodginsii,the size of the cones is a constraint on the reproductive renewal of the population.In this study,the molecular basis of the influence of cone siz...As the reproductive organ of the endangered species Fokienia hodginsii,the size of the cones is a constraint on the reproductive renewal of the population.In this study,the molecular basis of the influence of cone size on F.hodginsii was elucidated by comparing the phenotype,biochemistry,and transcriptome of two cultivars of F.hodginsii(‘FJ431’and‘FJ415’).The two cultivars differed significantly in cone size,with FJ431 having a significantly larger cone size and weight than FJ415,1.32 and 1.90 times that of FJ415,respectively.RNA-Seq analysis of both cultivars retrieved 75,940 genes whose approximate functions were classified as the pathway of response to endogenous stimulus and response to hormone and showed significant differences in the auxin-activated signaling pathway,particularly the MAPK signaling pathway-plant.Furthermore,the endogenous IAA content was significantly higher in FJ431 than in FJ415,and 1.58 and 1.29 times more IAA was present in immature and mature cones,respectively.Moreover,exogenous IAA treatment significantly induced the expression of the MAPK pathway-related gene TRINITY_DN10564_c0_g1 and significantly inhibited the expression of the MAPK pathwayrelated gene TRINITY_DN17056_c0_g1.Our work suggests that IAA can affect the cone size of F.hodginsii,most probably through the MAPK pathway.This has high theoretical and practical significance for the improvement of genetic breeding and the further cultivation of quality germplasm resources of F.hodginsii.展开更多
Light is an environmental signaling,whereas Aux/IAA proteins and Auxin Response Factors(ARFs)are regulators of auxin signalling.Aux/IAA proteins are unstable,and their degradation dependents on 26S ubiquitin-proteasom...Light is an environmental signaling,whereas Aux/IAA proteins and Auxin Response Factors(ARFs)are regulators of auxin signalling.Aux/IAA proteins are unstable,and their degradation dependents on 26S ubiquitin-proteasome and is promoted by Auxin.Auxin binds directly to a SCF-type ubiquitin-protein ligase,TIR1,facilitates the interaction between Aux/IAA proteins and TIR1,and then the degradation of Aux/IAA proteins.A few studies have reported that some ARFs are also unstable proteins,and their degradation is also mediated by 26S proteasome.In this study,by using of antibodies recognizing endogenous ARF7 proteins,we found that protein stability of ARF7 was affected by light.By expressing MYC tagged ARF activators in protoplasts,we found that degradation of ARF7 was inhibited by 26 proteasome inhibitors.In addition,at least ARF5 and ARF19 were also unstable proteins,and degradation of ARF5 via 26S proteasome was further confirmed by using stable transformed plants overexpressing ARF5 with a GUS tag.展开更多
Auxin has been suggested to play an essential role in regulating apple fruit maturation and ripening, though the molecular function of auxin and its interaction with ethylene during apple fruit development are largely...Auxin has been suggested to play an essential role in regulating apple fruit maturation and ripening, though the molecular function of auxin and its interaction with ethylene during apple fruit development are largely unknown. To understand the function of auxin during apple fruit maturation and ripening, auxin efflux carrier and IAA-amido synthetase encoding genes were identified from the apple genome based on the results of previous microarray analysis. The expression patterns of these genes were analyzed using qRT-PCR during 10 - 12 weeks of fruit maturation for two apple cultivars: “Golden Delicious” (GD) and “Cripps Pink” (CP), which have the distinct patterns of maturation progression. Our results showed that the expressions of auxin efflux carrier and IAA-amido synthetase genes have a correlation with the timing of ethylene biosynthesis pathway activation in both cultivars. The earlier and stronger expression of MdGH3.102 and MdAECFP1 in the fruit of GD, a mid-season cultivar, correlates with the earlier activation of a pre-climacteric ethylene biosynthesis gene of MdACS3, compared with that in CP, a late-ripening apple cultivar. Results of exogenous IAA treatment indicated that the expression patterns of the genes were regulated in a fruit maturity dependent manner. Our results suggested that the dynamics of the auxin level in apple fruit cortex could be one of the key factors influencing the timing of ethylene biosynthesis pathway activation and consequently contributed to the control of the apple maturation progression.展开更多
The potential of Plant Growth Promoting Rhizobacteria(PGPR)has been demonstrated in the case of plant inoculation with bacteria of the genus Azospirillum which improves yield.A.brasilense produces a wide variety of mo...The potential of Plant Growth Promoting Rhizobacteria(PGPR)has been demonstrated in the case of plant inoculation with bacteria of the genus Azospirillum which improves yield.A.brasilense produces a wide variety of molecules,including the natural auxin indole-3-acetic acid(IAA),as well as other phytoregulators.However,several studies have suggested that auxin induces changes in plant development during their interaction with the bacteria.The effects of A.brasilense Sp245 on the development of Arabidopsis thaliana root were investigated to help explain the molecular basis of the interaction.The results obtained showed a decrease in primary root length from the first day and remained so throughout the exposure,accompanied by a stimulation of initiation and maturation of lateral root primordia and an increase of lateral roots.An enhanced auxin response was evident in the vascular tissue and lateral root meristems of inoculated plants.However,after five days of bacterization,the response disappeared in the primary root meristems.The role of polar auxin transport(PAT)in auxins relocation involved the PGP1,AXR4-1,and BEN2 proteins,which apparently mediated A.brasilense-induced root branching of Arabidopsis seedlings.展开更多
ENOX (ECTO-NOX) proteins of the external surface of the plasma membrane catalyze oxidation of both NADH and hydroquinones and protein disulfide-thiol interchange. They exhibit both prion-like and time-keeping (clock) ...ENOX (ECTO-NOX) proteins of the external surface of the plasma membrane catalyze oxidation of both NADH and hydroquinones and protein disulfide-thiol interchange. They exhibit both prion-like and time-keeping (clock) properties. The oxidative and interchange activities alternate to generate a regular period of 24 min in length. Here we report the cloning, expression and characterization of a constitutive plant ENOX protein activated by both natural (Indole-3-acetic acid, IAA) and synthetic (2,4-dichlorophenoxyacetic acid, 2,4-D) auxin plant growth regulators with an optimum of about 1 μM, higher concentrations being less effective. The gene encoding the 213 amino acid protein (ABP20) is found in EMBL accession number U81162. Functional motifs characteristic of ENOX1 proteins, previously identified by site-directed mutagenesis, are present in the candidate auxin-activated ENOX (dNOX, ENOX5), including adenine nucleotide and copper binding motifs along with essential cysteines and a motif having homology with a previously identified auxin-binding motif. Periodicity was exhibited by both the oxidative and protein disulfide-thiol inter-change activities as is characteristic for other ENOX proteins. Activity was blocked by the ENOX2-specific quassinoid inhibitor glaucarubolone and other ENOX2 inhibitors but not by the ENOX1-specific quassinoid inhibitor simalikalactone D. Activity required both auxin and bound copper. The inactive auxin 2,3-D was without effects.展开更多
TiO_(2) nanoparticles(nano-TiO_(2))are widely used in the world,and a considerable amount of nano-TiO_(2) is released into the environment,with toxic effects on organisms.In the various species of higher plants,growth...TiO_(2) nanoparticles(nano-TiO_(2))are widely used in the world,and a considerable amount of nano-TiO_(2) is released into the environment,with toxic effects on organisms.In the various species of higher plants,growth,including seed germination,root elongation,and biomass accumulation,is affected by nano-TiO_(2).However,the underlying molecular mechanisms remain to be elucidated.In this study,we observed that nano-TiO_(2) promoted root elongation in a dose-dependent manner.Furthermore,we found that nano-TiO_(2) elevated auxin accumulation in the root tips of the auxin marker lines DII-VENUS and DR5::GUS,and,correspondingly,quantitative real-time PCR analysis revealed that nano-TiO_(2) increased the expression levels of auxin biosynthesis-and transport-related genes.GFP fluorescence observation using transgenic PIN2-GFP indicated that nano-TiO_(2) promoted root growth by inducing PIN2 accumulation.Thus,we propose that nano-TiO_(2) promote root growth in Arabidopsis thaliana by altering the expression levels of auxin biosynthesis-and transport-related genes.展开更多
根据测序获得的1条260 bp cDNA片段,通过预测发现其包含小麦植物生长素(AUXIN)基因的部分编码序列,通过电子延伸、设计引物,从小麦Mardler/7*百农3217的cDNA中扩增获得一条608 bp的cDNA片段,该基因序列数据库(GenBank)登录号为AY902381...根据测序获得的1条260 bp cDNA片段,通过预测发现其包含小麦植物生长素(AUXIN)基因的部分编码序列,通过电子延伸、设计引物,从小麦Mardler/7*百农3217的cDNA中扩增获得一条608 bp的cDNA片段,该基因序列数据库(GenBank)登录号为AY902381(基因)和(蛋白)。编码202个氨基酸,预计蛋白的分子量为23.0 kDa,等电点为9.93。利用已经分离的小麦生长素(AUXIN)基因的保守序列为检索序列,对小麦和水稻中的AUXIN基因家族成员进行分析,利用这些基因编码蛋白序列构建系统发生树,查找在GenBank的EST数据库中查找这些基因的ESTs表达序列,分析了这些基因在细胞中的定位情况和蛋白结构的相似性,根据已知相似基因的功能,分析该基因有进一步深入研究的必要。展开更多
Auxin signaling plays a significant role in the whole process of plant growth and development from embryogenesis to senescence.Auxin response factors(ARFs) are reported to regulate the expression of auxin response gen...Auxin signaling plays a significant role in the whole process of plant growth and development from embryogenesis to senescence.Auxin response factors(ARFs) are reported to regulate the expression of auxin response genes by binding to auxin response elements.ARF is the most critical transcription factor family which has been released in most species,but few reports in strawberry.In this study,the structure characterization of 12 FvARF genes in strawberry,their expression patterns at different development stages,different organizations,and different indole-3-acetic acid(IAA) treatments were analyzed.The expression of 12 FvARFs was found in all experiment tissues and showed almost the same trend during fruit development.All FvARFs respond to the treatment of IAA.Our study provides comprehensive information on ARF family in strawberry,including gene structures,chromosome locations,phylogenetic relationships and expression patterns.The information on FvARF genes paves the way for future research on strawberry ARF genes.展开更多
Auxin regulates cell division and elongation of the primordial cells through its concentration and then shaped the plant architecture. Cell division and elongation form the internode of soybean and result in different...Auxin regulates cell division and elongation of the primordial cells through its concentration and then shaped the plant architecture. Cell division and elongation form the internode of soybean and result in different plant heights and lodging resistance. Yet the mechanisms behind are unclear in soybean. To elucidate the mechanism of the concentration difference of auxin related to stem development in soybean, samples of apical shoot, elongation zone, and mature zone from the developing stems of soybean seedlings, Charleston, were harvested and measured for auxin concentration distributions and metabolites to identify the common underlying mechanisms responsible for concentration difference of auxin. Distribution of indole-3-acetic acid(IAA), indole-3-butyric acid(IBA), and methylindole-3-acetic acid(Me-IAA) were determined and auxin concentration distributions were found to have a complex regulation mechanism. The concentrations of IAA and Me-IAA in apical shoot were significantly different between elongation zone and mature zone resulting in an IAA gradient. Tryptophan dependent pathway from tryptamine directly to IAA or through indole-3-acetonitrile to IAA and from indole-3-propionic acid(IPA) to IAA were three primary IAA synthesis pathways. Moreover, some plant metabolites from flavonoid and phenylpropanoid synthesis pathways showed similar or reverse gradient and should involve in auxin homeostasis and concentration difference. All the data give the first insight in the concentration difference and homeostasis of auxin in soybean seedlings and facilitate a deeper understanding of the molecular mechanism of stem development and growth. The gathered information also helps to elucidate how plant height is formed in soybean and what strategy should be adopted to regulate the lodging resistance in soybean.展开更多
The effects of auxins and media on callus induction from the mature and immature embryos of Chinese spring wheat (Triticum aestivum L.) varieties were investigated. It was found that genotype, medium, auxin source a...The effects of auxins and media on callus induction from the mature and immature embryos of Chinese spring wheat (Triticum aestivum L.) varieties were investigated. It was found that genotype, medium, auxin source and concentration had the significant effects on the induction of embryogenic callus, explants germination and the increment of callus fresh weight. For immature embryos cultured on MS medium, 2 mg L^-1 of 2, 4-D was optimal, and the highest frequency of embryogenic callus (33.50%) was observed. For the mature embryos on N6 medium, 4 mg L^-1 of 2, 4-D was optimal. The frequency of embryogenic callus and increment of callus fresh weight on 2, 4, 5-T media were higher than those on 2, 4-D media, and in the presence of 2, 4, 5-T the precocious germination of explants for all genotypes were significantly suppressed. These results indicated that 2, 4, 5-T was superior to 2, 4-D and NAA in the culture of immature embryos. This is the first report about the effect of 2, 4, 5-T and NAA on wheat tissue culture, particularly in comparison with 2, 4-D in detail.展开更多
Pak choi is a low-temperature vernalized plant that readily undergoes premature bolting during spring, but little is known about the governing molecular regulation of vernalization. In order to enhance our understandi...Pak choi is a low-temperature vernalized plant that readily undergoes premature bolting during spring, but little is known about the governing molecular regulation of vernalization. In order to enhance our understanding of mechanism about premature bolting, we discussed the relationship between auxin(indole-3-acetic acid, IAA) and flowering of pak choi. During vernalization, hormone metabolism is an important regulatory pathway, and IAA plays a specific role. IAA metabolism has been studied in Arabidopsis thaliana and other plants, but not in pak choi. In this paper, the IAA content in pak choi shoot apices during vernalization and different growth stages was compared. The IAA content decreased significantly after low-temperature treatment(4 °C) and then increased rapidly during vegetative growth. During floral bud initiation,the IAA content decreased rapidly and was the lowest. Expressions of genes encoding key IAA metabolic enzymes were analyzed, and a major synthetase-encoding gene was downregulated, while a key degrading enzyme-encoding gene was upregulated during each comparison period,resulting in decreased IAA content. Expressions of four genes(Bra034975, Bra030246, Bra012239 and Bra040296) were consistent with changes in the IAA content. The functions of differentially expressed genes(DEGs) were analyzed, and 15 DEGs were found to be related to IAA metabolism.The findings illuminated the molecular mechanism regulating IAA content during vernalization in pak choi.展开更多
Polar auxin transport plays a divergent role in plant growth and developmental processes including rootand embryo development, vascular pattern formation and cell elongation. Recently isolated Arabidopsispin gene fami...Polar auxin transport plays a divergent role in plant growth and developmental processes including rootand embryo development, vascular pattern formation and cell elongation. Recently isolated Arabidopsispin gene family was believed to encode a component of auxin efflux carrier (Galweiler et al, 1998). Basedon the Arabidopsis pin1 sequence we have isolated a Brassica juncea cDNA (designated Bjpinl), whichencoded a 70-kDa putative auxin efflux carrier. Deduced BjPIN1 shared 65% identities at protein level withAtPIN1 and was highly homologous to other putative PIN proteins of Arabidopsis (with highest homologyto AtPIN3). Hydrophobic analysis showed similar structures between BjPIN1 and AtPIN proteins. Presenceof 6 exons (varying in size between 65 bp and 1229 bp) and 5 introns (sizes between 89 bp and 463 bp)in the genomic fragment was revealed by comparing the genomic and cDNA sequences. Northern blotanalysis indicated that Bjpinl was expressed in most of the tissues tested, with a relatively higher levelof transcript in flowers and a lower level in root tissues. Promoter-reporter gene fusion studies furtherrevealed the expression of Bjpinl in the mature pollen grains, young seeds, root tip, leaf vascular tissue andtrace bundle, stem epidermis, cortex and vascular cells. BjPIN1 was localized on the plasma membraneas demonstrated through fusion expression of green fluorescent protein (GFP). Auxin efflux carrier activitywas elevated in transgenic Arabidopsis expressing BjPIN1.展开更多
Based on the sequence information of Arabidopsis PIN1, two cDNAs encoding PIN homologues fromBrassica juncea, Bjpin2 and Bjpin3, were isolated through cDNA library screening. Bjpin2 and Bjpin3encoded proteins containi...Based on the sequence information of Arabidopsis PIN1, two cDNAs encoding PIN homologues fromBrassica juncea, Bjpin2 and Bjpin3, were isolated through cDNA library screening. Bjpin2 and Bjpin3encoded proteins containing 640 and 635 amino acid residues, respectively, which shared 97.5% identities witheach other and were highly homologous to Arabidopsis PIN1, PIN2 and other putative PIN proteins. BjPIN2and BjPIN3 had similar structures as AtPIN proteins. Northern blot analysis indicated that Bjpin2 wasexpressed in stem, leaf and floral tissues, while Bjpin3 was expressed predominantly in stem and hypocotyls.Two promoter fragments of pin genes, Bjpin-X and Bjpin-Z, were isolated by 'genome walking' techniqueusing primers at 5'-end of pin cDNA. Promoter-gus fusion studies revealed the GUS activities driven byBjpin-X were at internal side of xylem and petal; while those driven by Bjpin-Z were detected at leaf vein,epidermal cell and cortex of stem, vascular tissues and anther. Results of the pin genes with differentexpression patterns in B. juncea suggested the presence of a gene family.展开更多
基金supported by the National Key Research and Development Program of China(Grant No.2018YFD1000307)the National Natural Science Foundation of China(Grant No.32172529)+2 种基金the Special Funds for Major Scientific and Technological Innovation from Shaanxi Province(Grant No.2020zdzx03-0101)the Earmarked Fund for China Agriculture Research System(Grant No.CARS-27)China Postdoctoral Science Foundation(Grant Nos.2017M610657,2018T111108)。
文摘Auxin is throughout the entire life process of plants and is involved in the crosstalk with other hormones,yet its role in apple disease resistance remains unclear.In this study,we investigated the function of auxin/indole-3-acetic acid(IAA)gene Md IAA24 overexpression in enhancing apple resistance to Glomerella leaf spot(GLS)caused by Colletotrichum fructicola(Cf).Analysis revealed that,upon Cf infection,35S::Md IAA24 plants exhibited enhanced superoxide dismutase(SOD)and peroxidase(POD)activity,as well as a greater amount of glutathione(reduced form)and ascorbic acid accumulation,resulting in less H_(2)O_(2)and superoxide anion(O_(2)^(-))in apple leaves.Furthermore,35S::Md IAA24 plants produced more protocatechuic acid,proanthocyanidins B1,proanthocyanidins B2 and chlorogenic acid when infected with Cf.Following Cf infection,35S::Md IAA24 plants presented lower levels of IAA and jasmonic acid(JA),but higher levels of salicylic acid(SA),along with the expression of related genes.The overexpression of Md IAA24 was observed to enhance the activity of chitinase andβ-1,3-glucanase in Cfinfected leaves.The results indicated the ability of Md IAA24 to regulate the crosstalk between IAA,JA and SA,and to improve reactive oxygen species(ROS)scavenging and defense-related enzymes activity.This jointly contributed to GLS resistance in apple.
基金Open Project of Jiangsu Key Laboratory for Eco-Agricultural Biotechnology around Hongze Lake,Grant Number HZHLAB2201.
文摘Carbon monoxide(CO)and nitric oxide(NO)are signal molecules that enhance plant adaptation to environmental stimuli.Auxin is an essential phytohormone for plant growth and development.CO and NO play crucial roles in modulating the plant’s response to iron deficiency.Iron deficiency leads to an increase in the activity of heme oxygenase(HO)and the subsequent generation of CO.Additionally,it alters the polar subcellular distribution of Pin-Formed 1(PIN1)proteins,resulting in enhanced auxin transport.This alteration,in turn,leads to an increase in NO accumulation.Furthermore,iron deficiency enhances the activity of ferric chelate reductase(FCR),as well as the expression of the Fer-like iron deficiency-induced transcription factor 1(FIT)and the ferric reduction oxidase 2(FRO2)genes in plant roots.Overexpression of the long hypocotyl 1(HY1)gene,which encodes heme oxygenase,or the CO donor treatment resulted in enhanced basipetal auxin transport,higher FCR activity,and the expression of FIT and FRO2 genes under Fe deficiency.Here,a potential mechanism is proposed:CO and NO interact with auxin to address iron deficiency stress.CO alters auxin transport,enhancing its accumulation in roots and up-regulating key iron-related genes like FRO2 and IRT1.Elevated auxin levels affect NO signaling,leading to greater sensitivity in root development.This interplay promotes FCR activity,which is crucial for iron absorption.Together,these molecules enhance iron uptake and root growth,revealing a novel aspect of plant physiology in adapting to environmental stress.
基金This work was financially supported by the joint funds of National Natural Science Foundation of China(U21A20232)the Natural Science Foundation of China(32072621,32002088,31870676)Collegiate Collaborative Innovation Foundation of Anhui Province(GXXT-2020-081).
文摘Subgroup 4(Sg4)members of the R2R3-MYB are generally known as negative regulators of the phenylpropanoid pathway in plants.Our previous research showed that a R2R3-MYB Sg4 member from Camellia sinensis(CsMYB4a)inhibits expression of some genes in the phenylpropanoid pathway,but its physiological function in the tea plant remained unknown.Here,CsMYB4a was found to be highly expressed in anther and filaments,and participated in regulating filament growth.Transcriptome analysis and exogenous auxin treatment showed that the target of CsMYB4a might be the auxin signal pathway.Auxin/indole-3-acetic acid 4(AUX/IAA4),a repressor in auxin signal transduction,was detected from a yeast two-hybrid screen using CsMYB4a as bait.Gene silencing assays showed that both CsIAA4 and CsMYB4a regulate filament growth.Tobacco plants overexpressing CsIAA4 were insensitive to exogenous a-NAA,consistent with overexpression of CsMYB4a.Protein-protein interaction experiments revealed that CsMYB4a interacts with N-terminal of CsIAA4 to prevent CsIAA4 degradation.Knock out of the endogenous NtIAA4 gene,a CsIAA4 homolog,in tobacco alleviated filament growth inhibition and a-NAA insensitivity in plants overexpressing CsMYB4a.All results strongly suggest that CsMYB4a works synergistically with CsIAA4 and participates in regulation of the auxin pathway in stamen.
基金the National Natural Science Foundation of China(31972378)the Shandong Province Key R&D Program+1 种基金China(2021CXGC010802)the China Agriculture Research System of MOF and MARA(CARS-27)。
文摘Under appropriate culture conditions,plant cells can regenerate new organs or even whole plants.De novo organ regeneration is an excellent biological system,which usually requires additional growth regulators,including auxin and cytokinin.Nitrate is an essential nutrient element for plant vegetative and reproductive development.It has been reported that nitrate is involved in auxin biosynthesis and transport throughout the growth and development of plants.In this study,we demonstrated that the ectopic expression of the MdNLP7 transcription factor in Arabidopsis could regulate the regeneration of root explants.MdNLP7 mainly participated in the regulation of callus formation,starting with pericycle cell division,and mainly affected auxin distribution and accumulation in the regulation process.Moreover,MdNLP7 upregulated the expression of genes related to auxin biosynthesis and transport in the callus formation stage.The results demonstrated that MdNLP7 may play a role in the nitrate-modulated regeneration of root explants.Moreover,the results revealed that nitrate–auxin crosstalk is required for de novo callus initiation and clarified the mechanisms of organogenesis.
基金supported by grants from the Natural Science Foundation of Chongqing,China(cstc2020jcyj-msxm0539,cstc2015jcyjA80008)the National College Students Innovation and Entrepreneurship Training Program from the Ministry of Education,China(202110635082)the National Natural Science Foundation of China(32171964,31171178).
文摘The trichomes of rice leaves are formed by the differentiation and development of epidermal cells.Plant trichomes play an important role in stress resistance and protection against direct ultraviolet irradiation.However,the development of rice trichomes remains poorly understood.In this study,we conducted ethylmethane sulfonate(EMS)-mediated mutagenesis on the wild-type(WT)indica rice‘Xida 1B’.Phenotypic analysis led to the screening of a mutant that is defective in trichome development,designated lhl1(less hairy leaf 1).We performed map-based cloning and localized the mutated gene to the 70-kb interval between the molecular markers V-9 and V-10 on chromosome 2.The locus LOC_Os02g25230 was identified as the candidate gene by sequencing.We constructed RNA interference(LHL1-RNAi)and overexpression lines(LHL1-OE)to verity the candidate gene.The leaves of the LHL1-RNAi lines showed the same trichome developmental defects as the lhl1 mutant,whereas the trichome morphology on the leaf surface of the LHL1-OE lines was similar to that of the WT,although the number of trichomes was significantly higher.Quantitative real-time PCR(RT-qPCR)analysis revealed that the expression levels of auxin-related genes and positive regulators of trichome development in the lhl1 mutant were down-regulated compared with the WT.Hormone response analysis revealed that LHL1 expression was affected by auxin.The results indicate that the influence of LHL1 on trichome development in rice leaves may be associated with an auxin pathway.
基金supported by the Science and Technology Innovation Program of Hunan Province,China(2020RC2057).
文摘Upland cotton(Gossypium hirsutum L.)is the most important natural textile fiber crop worldwide.Plant height(PH)is a significant component of plant architecture,strongly influencing crop cultivation patterns,overall yield,and economic coefficient.However,cotton genes regulating plant height have not been fully identified.Previously,an HD-Zip gene(GhHB12)was isolated and characterized in cotton,which regulates the abiotic and biotic stress responses and the growth and development processes.In this study,we showed that GhHB12 was induced by auxin.Moreover,overexpression of GhHB12 induces the expression of HY5,ATH1,and HAT4,represses the spatial-temporal distribution,polar transport,and signaling of auxin,alters the expression of genes involved in cell wall expansion,and restrains the plant height in cotton.These results suggest a role of GhHB12 in regulating cotton plant height,which could be achieved by affecting the auxin signaling and cell wall expansion.
基金supported by the National Natural Science Foundation of China(Grant Nos.32172538,31972378)China Agriculture Research System of MOF and MARA(Grant CARS-27).
文摘The auxin receptor(TIR1/AFBs)family encodes the F-box protein subunit,which is involved in the formation of the E3 ubiquitin ligase SCFTIR1/AFBs complex,a key component of the auxin signaling pathway.However,there are few studies on the auxin receptor family in apple(Malus×domestica).In this study,eight MdAFBs were identified,and phylogenetic analysis showed that they were classified into four groups and distributed on eight chromosomes.Herein,a comprehensive analysis of the MdAFB gene family was conducted to identify cis-acting elements,gene structures,protein structures,aligned sequences,conserved motifs,conserved amino acids,and the protein–protein interaction network.The results of yeast two-hybrid assays showed that MdAFB1 interacted with three auxin repressor proteins.The results of qRT-PCR showed that MdAFB1 responded to osmotic and salt stress.The overexpression of MdAFB1 increased osmotic and salt resistance in apple calli,and the ectopic expression of MdAFB1 enhanced osmotic and salt tolerance in Arabidopsis.This study provided a basis for the identification of auxin receptor genes in apple and their functions in mediating osmotic and salt stress.
基金The“Eagle Program”of Fujian Province,funded by the Department of Human Resources and Social Security of Fujian ProvinceThe“Fujian Cypress 1st Generation Core Breeding Population Construction Research”(No.2021R1010004),funded by the Department of Science and Technology of Fujian Province.
文摘As the reproductive organ of the endangered species Fokienia hodginsii,the size of the cones is a constraint on the reproductive renewal of the population.In this study,the molecular basis of the influence of cone size on F.hodginsii was elucidated by comparing the phenotype,biochemistry,and transcriptome of two cultivars of F.hodginsii(‘FJ431’and‘FJ415’).The two cultivars differed significantly in cone size,with FJ431 having a significantly larger cone size and weight than FJ415,1.32 and 1.90 times that of FJ415,respectively.RNA-Seq analysis of both cultivars retrieved 75,940 genes whose approximate functions were classified as the pathway of response to endogenous stimulus and response to hormone and showed significant differences in the auxin-activated signaling pathway,particularly the MAPK signaling pathway-plant.Furthermore,the endogenous IAA content was significantly higher in FJ431 than in FJ415,and 1.58 and 1.29 times more IAA was present in immature and mature cones,respectively.Moreover,exogenous IAA treatment significantly induced the expression of the MAPK pathway-related gene TRINITY_DN10564_c0_g1 and significantly inhibited the expression of the MAPK pathwayrelated gene TRINITY_DN17056_c0_g1.Our work suggests that IAA can affect the cone size of F.hodginsii,most probably through the MAPK pathway.This has high theoretical and practical significance for the improvement of genetic breeding and the further cultivation of quality germplasm resources of F.hodginsii.
文摘Light is an environmental signaling,whereas Aux/IAA proteins and Auxin Response Factors(ARFs)are regulators of auxin signalling.Aux/IAA proteins are unstable,and their degradation dependents on 26S ubiquitin-proteasome and is promoted by Auxin.Auxin binds directly to a SCF-type ubiquitin-protein ligase,TIR1,facilitates the interaction between Aux/IAA proteins and TIR1,and then the degradation of Aux/IAA proteins.A few studies have reported that some ARFs are also unstable proteins,and their degradation is also mediated by 26S proteasome.In this study,by using of antibodies recognizing endogenous ARF7 proteins,we found that protein stability of ARF7 was affected by light.By expressing MYC tagged ARF activators in protoplasts,we found that degradation of ARF7 was inhibited by 26 proteasome inhibitors.In addition,at least ARF5 and ARF19 were also unstable proteins,and degradation of ARF5 via 26S proteasome was further confirmed by using stable transformed plants overexpressing ARF5 with a GUS tag.
文摘Auxin has been suggested to play an essential role in regulating apple fruit maturation and ripening, though the molecular function of auxin and its interaction with ethylene during apple fruit development are largely unknown. To understand the function of auxin during apple fruit maturation and ripening, auxin efflux carrier and IAA-amido synthetase encoding genes were identified from the apple genome based on the results of previous microarray analysis. The expression patterns of these genes were analyzed using qRT-PCR during 10 - 12 weeks of fruit maturation for two apple cultivars: “Golden Delicious” (GD) and “Cripps Pink” (CP), which have the distinct patterns of maturation progression. Our results showed that the expressions of auxin efflux carrier and IAA-amido synthetase genes have a correlation with the timing of ethylene biosynthesis pathway activation in both cultivars. The earlier and stronger expression of MdGH3.102 and MdAECFP1 in the fruit of GD, a mid-season cultivar, correlates with the earlier activation of a pre-climacteric ethylene biosynthesis gene of MdACS3, compared with that in CP, a late-ripening apple cultivar. Results of exogenous IAA treatment indicated that the expression patterns of the genes were regulated in a fruit maturity dependent manner. Our results suggested that the dynamics of the auxin level in apple fruit cortex could be one of the key factors influencing the timing of ethylene biosynthesis pathway activation and consequently contributed to the control of the apple maturation progression.
基金supported by the Coordinación de la Investigación Científica UMSNH.E.C.-F.and J.A.-R.were fellows of CONACYT-México.
文摘The potential of Plant Growth Promoting Rhizobacteria(PGPR)has been demonstrated in the case of plant inoculation with bacteria of the genus Azospirillum which improves yield.A.brasilense produces a wide variety of molecules,including the natural auxin indole-3-acetic acid(IAA),as well as other phytoregulators.However,several studies have suggested that auxin induces changes in plant development during their interaction with the bacteria.The effects of A.brasilense Sp245 on the development of Arabidopsis thaliana root were investigated to help explain the molecular basis of the interaction.The results obtained showed a decrease in primary root length from the first day and remained so throughout the exposure,accompanied by a stimulation of initiation and maturation of lateral root primordia and an increase of lateral roots.An enhanced auxin response was evident in the vascular tissue and lateral root meristems of inoculated plants.However,after five days of bacterization,the response disappeared in the primary root meristems.The role of polar auxin transport(PAT)in auxins relocation involved the PGP1,AXR4-1,and BEN2 proteins,which apparently mediated A.brasilense-induced root branching of Arabidopsis seedlings.
文摘ENOX (ECTO-NOX) proteins of the external surface of the plasma membrane catalyze oxidation of both NADH and hydroquinones and protein disulfide-thiol interchange. They exhibit both prion-like and time-keeping (clock) properties. The oxidative and interchange activities alternate to generate a regular period of 24 min in length. Here we report the cloning, expression and characterization of a constitutive plant ENOX protein activated by both natural (Indole-3-acetic acid, IAA) and synthetic (2,4-dichlorophenoxyacetic acid, 2,4-D) auxin plant growth regulators with an optimum of about 1 μM, higher concentrations being less effective. The gene encoding the 213 amino acid protein (ABP20) is found in EMBL accession number U81162. Functional motifs characteristic of ENOX1 proteins, previously identified by site-directed mutagenesis, are present in the candidate auxin-activated ENOX (dNOX, ENOX5), including adenine nucleotide and copper binding motifs along with essential cysteines and a motif having homology with a previously identified auxin-binding motif. Periodicity was exhibited by both the oxidative and protein disulfide-thiol inter-change activities as is characteristic for other ENOX proteins. Activity was blocked by the ENOX2-specific quassinoid inhibitor glaucarubolone and other ENOX2 inhibitors but not by the ENOX1-specific quassinoid inhibitor simalikalactone D. Activity required both auxin and bound copper. The inactive auxin 2,3-D was without effects.
基金This work is supported by the National Science Foundation of China(No.31970289).
文摘TiO_(2) nanoparticles(nano-TiO_(2))are widely used in the world,and a considerable amount of nano-TiO_(2) is released into the environment,with toxic effects on organisms.In the various species of higher plants,growth,including seed germination,root elongation,and biomass accumulation,is affected by nano-TiO_(2).However,the underlying molecular mechanisms remain to be elucidated.In this study,we observed that nano-TiO_(2) promoted root elongation in a dose-dependent manner.Furthermore,we found that nano-TiO_(2) elevated auxin accumulation in the root tips of the auxin marker lines DII-VENUS and DR5::GUS,and,correspondingly,quantitative real-time PCR analysis revealed that nano-TiO_(2) increased the expression levels of auxin biosynthesis-and transport-related genes.GFP fluorescence observation using transgenic PIN2-GFP indicated that nano-TiO_(2) promoted root growth by inducing PIN2 accumulation.Thus,we propose that nano-TiO_(2) promote root growth in Arabidopsis thaliana by altering the expression levels of auxin biosynthesis-and transport-related genes.
文摘根据测序获得的1条260 bp cDNA片段,通过预测发现其包含小麦植物生长素(AUXIN)基因的部分编码序列,通过电子延伸、设计引物,从小麦Mardler/7*百农3217的cDNA中扩增获得一条608 bp的cDNA片段,该基因序列数据库(GenBank)登录号为AY902381(基因)和(蛋白)。编码202个氨基酸,预计蛋白的分子量为23.0 kDa,等电点为9.93。利用已经分离的小麦生长素(AUXIN)基因的保守序列为检索序列,对小麦和水稻中的AUXIN基因家族成员进行分析,利用这些基因编码蛋白序列构建系统发生树,查找在GenBank的EST数据库中查找这些基因的ESTs表达序列,分析了这些基因在细胞中的定位情况和蛋白结构的相似性,根据已知相似基因的功能,分析该基因有进一步深入研究的必要。
基金financially supported by the National Natural Science Foundation of China(31872069)the Natural Science Foundation of Liaoning Province,China(201602659)+1 种基金the Liaoning BaiQianWan Talents Program,China(2016921067)the Program for Excellent Talents in University of Liaoning Province,China(LJQ2014069)
文摘Auxin signaling plays a significant role in the whole process of plant growth and development from embryogenesis to senescence.Auxin response factors(ARFs) are reported to regulate the expression of auxin response genes by binding to auxin response elements.ARF is the most critical transcription factor family which has been released in most species,but few reports in strawberry.In this study,the structure characterization of 12 FvARF genes in strawberry,their expression patterns at different development stages,different organizations,and different indole-3-acetic acid(IAA) treatments were analyzed.The expression of 12 FvARFs was found in all experiment tissues and showed almost the same trend during fruit development.All FvARFs respond to the treatment of IAA.Our study provides comprehensive information on ARF family in strawberry,including gene structures,chromosome locations,phylogenetic relationships and expression patterns.The information on FvARF genes paves the way for future research on strawberry ARF genes.
基金financially supported by the National Natural Science Foundation of China(31571693)the earmarked fund for China Agriculture Research System(CARS-04-04B)。
文摘Auxin regulates cell division and elongation of the primordial cells through its concentration and then shaped the plant architecture. Cell division and elongation form the internode of soybean and result in different plant heights and lodging resistance. Yet the mechanisms behind are unclear in soybean. To elucidate the mechanism of the concentration difference of auxin related to stem development in soybean, samples of apical shoot, elongation zone, and mature zone from the developing stems of soybean seedlings, Charleston, were harvested and measured for auxin concentration distributions and metabolites to identify the common underlying mechanisms responsible for concentration difference of auxin. Distribution of indole-3-acetic acid(IAA), indole-3-butyric acid(IBA), and methylindole-3-acetic acid(Me-IAA) were determined and auxin concentration distributions were found to have a complex regulation mechanism. The concentrations of IAA and Me-IAA in apical shoot were significantly different between elongation zone and mature zone resulting in an IAA gradient. Tryptophan dependent pathway from tryptamine directly to IAA or through indole-3-acetonitrile to IAA and from indole-3-propionic acid(IPA) to IAA were three primary IAA synthesis pathways. Moreover, some plant metabolites from flavonoid and phenylpropanoid synthesis pathways showed similar or reverse gradient and should involve in auxin homeostasis and concentration difference. All the data give the first insight in the concentration difference and homeostasis of auxin in soybean seedlings and facilitate a deeper understanding of the molecular mechanism of stem development and growth. The gathered information also helps to elucidate how plant height is formed in soybean and what strategy should be adopted to regulate the lodging resistance in soybean.
基金the National High Tech R&D Program of China(863 Program,2003AA207100)the Foundation for the Author of National Excellent Doctoral Dissertation of P R China(200357) the Program forChangjiang Scholars and Innovative Research Team inUniversity(IRT0453).
文摘The effects of auxins and media on callus induction from the mature and immature embryos of Chinese spring wheat (Triticum aestivum L.) varieties were investigated. It was found that genotype, medium, auxin source and concentration had the significant effects on the induction of embryogenic callus, explants germination and the increment of callus fresh weight. For immature embryos cultured on MS medium, 2 mg L^-1 of 2, 4-D was optimal, and the highest frequency of embryogenic callus (33.50%) was observed. For the mature embryos on N6 medium, 4 mg L^-1 of 2, 4-D was optimal. The frequency of embryogenic callus and increment of callus fresh weight on 2, 4, 5-T media were higher than those on 2, 4-D media, and in the presence of 2, 4, 5-T the precocious germination of explants for all genotypes were significantly suppressed. These results indicated that 2, 4, 5-T was superior to 2, 4-D and NAA in the culture of immature embryos. This is the first report about the effect of 2, 4, 5-T and NAA on wheat tissue culture, particularly in comparison with 2, 4-D in detail.
基金supported by Shanxi Province Key Research and Development Program Key Projects (Grant No. 201703D211006 201703D211001-04-01)the Natural Science Foundation of Shanxi Province (Grant No. 201701D121101)
文摘Pak choi is a low-temperature vernalized plant that readily undergoes premature bolting during spring, but little is known about the governing molecular regulation of vernalization. In order to enhance our understanding of mechanism about premature bolting, we discussed the relationship between auxin(indole-3-acetic acid, IAA) and flowering of pak choi. During vernalization, hormone metabolism is an important regulatory pathway, and IAA plays a specific role. IAA metabolism has been studied in Arabidopsis thaliana and other plants, but not in pak choi. In this paper, the IAA content in pak choi shoot apices during vernalization and different growth stages was compared. The IAA content decreased significantly after low-temperature treatment(4 °C) and then increased rapidly during vegetative growth. During floral bud initiation,the IAA content decreased rapidly and was the lowest. Expressions of genes encoding key IAA metabolic enzymes were analyzed, and a major synthetase-encoding gene was downregulated, while a key degrading enzyme-encoding gene was upregulated during each comparison period,resulting in decreased IAA content. Expressions of four genes(Bra034975, Bra030246, Bra012239 and Bra040296) were consistent with changes in the IAA content. The functions of differentially expressed genes(DEGs) were analyzed, and 15 DEGs were found to be related to IAA metabolism.The findings illuminated the molecular mechanism regulating IAA content during vernalization in pak choi.
基金Studies were supported by "the National NaturalScience Foundation of China, No. 30070073", StateKey Project of Basic Research, No. G199901l604"and "National Natural Science Foundation of Pan-Deng". We thank Dr. Charles Brearley and JianXu for hel
文摘Polar auxin transport plays a divergent role in plant growth and developmental processes including rootand embryo development, vascular pattern formation and cell elongation. Recently isolated Arabidopsispin gene family was believed to encode a component of auxin efflux carrier (Galweiler et al, 1998). Basedon the Arabidopsis pin1 sequence we have isolated a Brassica juncea cDNA (designated Bjpinl), whichencoded a 70-kDa putative auxin efflux carrier. Deduced BjPIN1 shared 65% identities at protein level withAtPIN1 and was highly homologous to other putative PIN proteins of Arabidopsis (with highest homologyto AtPIN3). Hydrophobic analysis showed similar structures between BjPIN1 and AtPIN proteins. Presenceof 6 exons (varying in size between 65 bp and 1229 bp) and 5 introns (sizes between 89 bp and 463 bp)in the genomic fragment was revealed by comparing the genomic and cDNA sequences. Northern blotanalysis indicated that Bjpinl was expressed in most of the tissues tested, with a relatively higher levelof transcript in flowers and a lower level in root tissues. Promoter-reporter gene fusion studies furtherrevealed the expression of Bjpinl in the mature pollen grains, young seeds, root tip, leaf vascular tissue andtrace bundle, stem epidermis, cortex and vascular cells. BjPIN1 was localized on the plasma membraneas demonstrated through fusion expression of green fluorescent protein (GFP). Auxin efflux carrier activitywas elevated in transgenic Arabidopsis expressing BjPIN1.
文摘Based on the sequence information of Arabidopsis PIN1, two cDNAs encoding PIN homologues fromBrassica juncea, Bjpin2 and Bjpin3, were isolated through cDNA library screening. Bjpin2 and Bjpin3encoded proteins containing 640 and 635 amino acid residues, respectively, which shared 97.5% identities witheach other and were highly homologous to Arabidopsis PIN1, PIN2 and other putative PIN proteins. BjPIN2and BjPIN3 had similar structures as AtPIN proteins. Northern blot analysis indicated that Bjpin2 wasexpressed in stem, leaf and floral tissues, while Bjpin3 was expressed predominantly in stem and hypocotyls.Two promoter fragments of pin genes, Bjpin-X and Bjpin-Z, were isolated by 'genome walking' techniqueusing primers at 5'-end of pin cDNA. Promoter-gus fusion studies revealed the GUS activities driven byBjpin-X were at internal side of xylem and petal; while those driven by Bjpin-Z were detected at leaf vein,epidermal cell and cortex of stem, vascular tissues and anther. Results of the pin genes with differentexpression patterns in B. juncea suggested the presence of a gene family.