利用菌丝生长速率法测定了采自中国苹果主产区4省27个县市的苹果轮纹病菌(Botryospha eriaberengeriana f. sp. piricola)对多菌灵、亚胺唑和丙环唑的敏感性。结果表明,苹果轮纹病菌对多菌灵的敏感程度最高,其EC50平均值为(0.1415±...利用菌丝生长速率法测定了采自中国苹果主产区4省27个县市的苹果轮纹病菌(Botryospha eriaberengeriana f. sp. piricola)对多菌灵、亚胺唑和丙环唑的敏感性。结果表明,苹果轮纹病菌对多菌灵的敏感程度最高,其EC50平均值为(0.1415±0.0887)mg·L-1,对亚胺唑和丙环唑的次之,其EC50平均值分别为(0.1994±0.2431)和(0.2809±0.2607)mg·mL-1。对上述3种药剂间的交互抗性分析显示,多菌灵和亚胺唑、多菌灵和丙环唑之间没有交互抗性,而丙环唑和亚胺唑之间有交互抗性。但在田间已发现2株对多菌灵和丙环唑的敏感性都相对较低的菌株。展开更多
[ Objective ] The study aimed to explore the release conditions for the conidia of Botryosphaena berengeriana and understand the release dynamic of conidia. [Method] The systematical survey on the release conditions f...[ Objective ] The study aimed to explore the release conditions for the conidia of Botryosphaena berengeriana and understand the release dynamic of conidia. [Method] The systematical survey on the release conditions for the conidia of B. berengeriana were conducted in two growing seasons in 2008 and 2009, combined with the collection of meteorological data around conidia release period, the weather conditions causing large amount release of B. berengedana were analyzed. [ Result] During a growing season, the conidia of pathogen appeared several large release peaks. Under the suitable temperature, when the precipitation lasted for 4 h, the conidia of B. berengeriana began to release with large amount, the amount of conidia reached the peak after release and trended to be stable during 4 - 12 h, which significantly reduced after 24 h, tended to dis- appear after 36 h, and completely disappeared after 72 h. [Conclusion] The dominant factor affecting B. berengeriana conidia release in large a- mount was precipitation, while the lasting time of precipitation played a decisive role.展开更多
In order to get a great quantity of spores of Botryosphaeria berengeriana f.sp.piricola,several methods to promote sporulation of the pathogen were compared.The results showed that the wounded young apple fruits withi...In order to get a great quantity of spores of Botryosphaeria berengeriana f.sp.piricola,several methods to promote sporulation of the pathogen were compared.The results showed that the wounded young apple fruits within 60-day old was inoculated with mycelia of B.berengeriana f.sp.piricola and induced under the 365 nm-UV-light;when the brown lesion was shown,the young apple began to form pycnidia on the lesion around the inoculation point after 3-5 days irradiation with UV-light and extruded plenty of conidia.This method induced great more conidia than that of irradiating wounded mycelia with UV-light.However,mature apple seldom produced conidia when induced with the same method.展开更多
文摘利用菌丝生长速率法测定了采自中国苹果主产区4省27个县市的苹果轮纹病菌(Botryospha eriaberengeriana f. sp. piricola)对多菌灵、亚胺唑和丙环唑的敏感性。结果表明,苹果轮纹病菌对多菌灵的敏感程度最高,其EC50平均值为(0.1415±0.0887)mg·L-1,对亚胺唑和丙环唑的次之,其EC50平均值分别为(0.1994±0.2431)和(0.2809±0.2607)mg·mL-1。对上述3种药剂间的交互抗性分析显示,多菌灵和亚胺唑、多菌灵和丙环唑之间没有交互抗性,而丙环唑和亚胺唑之间有交互抗性。但在田间已发现2株对多菌灵和丙环唑的敏感性都相对较低的菌株。
基金Supported by State Apple Industry Technology System Project(nybcytx-08-04-01)~~
文摘[ Objective ] The study aimed to explore the release conditions for the conidia of Botryosphaena berengeriana and understand the release dynamic of conidia. [Method] The systematical survey on the release conditions for the conidia of B. berengeriana were conducted in two growing seasons in 2008 and 2009, combined with the collection of meteorological data around conidia release period, the weather conditions causing large amount release of B. berengedana were analyzed. [ Result] During a growing season, the conidia of pathogen appeared several large release peaks. Under the suitable temperature, when the precipitation lasted for 4 h, the conidia of B. berengeriana began to release with large amount, the amount of conidia reached the peak after release and trended to be stable during 4 - 12 h, which significantly reduced after 24 h, tended to dis- appear after 36 h, and completely disappeared after 72 h. [Conclusion] The dominant factor affecting B. berengeriana conidia release in large a- mount was precipitation, while the lasting time of precipitation played a decisive role.
文摘In order to get a great quantity of spores of Botryosphaeria berengeriana f.sp.piricola,several methods to promote sporulation of the pathogen were compared.The results showed that the wounded young apple fruits within 60-day old was inoculated with mycelia of B.berengeriana f.sp.piricola and induced under the 365 nm-UV-light;when the brown lesion was shown,the young apple began to form pycnidia on the lesion around the inoculation point after 3-5 days irradiation with UV-light and extruded plenty of conidia.This method induced great more conidia than that of irradiating wounded mycelia with UV-light.However,mature apple seldom produced conidia when induced with the same method.
