There are two types of Human Immunodeficiency Virus （HIV）： HIV-1 and HIV-2. HIV-1 dominates epidemics in many different parts of the world, and HIV-2 is principally responsible for the epidemic in westem Africa. In...There are two types of Human Immunodeficiency Virus （HIV）： HIV-1 and HIV-2. HIV-1 dominates epidemics in many different parts of the world, and HIV-2 is principally responsible for the epidemic in westem Africa. In China, Zeng et al have reported the first individual infected with HIV-1 in 1985. And in the 1990s, there was a severe epidemic involving the HIV-1 B＇ strain among people who sold blood and plasma in Henan, Hubei and adjacent provinces. To further study in HIV/AIDS vaccines and HIV-1 drug resistance for people in these regions, we need to construct an infectious HIV-1 B＇ molecular clone which is representative of the virus in these areas. To this end, we have isolated a HIV-1 B＇ virus from a child who was infected with HIV-1 from his mother in Hubei province, and have constructed a full-length clone from this genome.展开更多
Background The correlation between HIV-1 Nef-specific CD8 T-cell responses and markers of HIV-1 disease progression still remains unclear. This study analysed and compared the role of HIV-1 Nef-specific CD8 T-cell res...Background The correlation between HIV-1 Nef-specific CD8 T-cell responses and markers of HIV-1 disease progression still remains unclear. This study analysed and compared the role of HIV-1 Nef-specific CD8 T-cell responses in patients with different disease status. Methods Two groups of patients with HIV-1 subtype B infection were selected according to CD4 count and clinical manifestations： long-term nonprogresssors （LTNPs, n = 20） and advanced progressors （APs, CD4 count 〈500 cells/pl, n = 34）. Nef-specific CD8 T-cell responses were studied by interferon- T ELISpot assay against 3 pools of HIV-Nef peptides. Results Nef-specific CD8 T-cell responses did not correlate with viral load or CD4 count in all patients and no significant differences were found in the magnitude of Nef-specific CD8 T-cell responses between groups LTNPs and APs （670 SFC/106 peripheral blood mononuclear cells vs 1107 SFC/106 peripheral blood mononuclear cells, P = 0.255）. Further comparisons showed that there were also no significant correlations observed in group LTNPs, but Nef-specific CD8 T cells correlated negatively with viral load （r = -0.397, P = 0.020） and positively with CD4 count （r = 0.364, P = 0.034） in group APs. Conclusion These data suggest that different correlation patterns between Nef-specific CD8 T-cell responses and disease progression exist in LTNPs and APs. Although a negative association was observed with concurrent plasma HIV RNA in APs, Nef-specific CD8 T-cell responses might fail to play a protective role in different stages of HIV- 1 infection.展开更多
基金This study was supported by the grants from the National High Technology Program 863(2005AA219070)the Project 973 of the National Program of China(2005CB522903).
文摘There are two types of Human Immunodeficiency Virus （HIV）： HIV-1 and HIV-2. HIV-1 dominates epidemics in many different parts of the world, and HIV-2 is principally responsible for the epidemic in westem Africa. In China, Zeng et al have reported the first individual infected with HIV-1 in 1985. And in the 1990s, there was a severe epidemic involving the HIV-1 B＇ strain among people who sold blood and plasma in Henan, Hubei and adjacent provinces. To further study in HIV/AIDS vaccines and HIV-1 drug resistance for people in these regions, we need to construct an infectious HIV-1 B＇ molecular clone which is representative of the virus in these areas. To this end, we have isolated a HIV-1 B＇ virus from a child who was infected with HIV-1 from his mother in Hubei province, and have constructed a full-length clone from this genome.
基金This study was funded by the National Nature Science Foundation of China (No. 30571641) and INCO: Phase 1/2 Clinical Testing of Clade C-based HIV-1 Candidate Vaccines in China (No. ICA4-2001-10148).
文摘Background The correlation between HIV-1 Nef-specific CD8 T-cell responses and markers of HIV-1 disease progression still remains unclear. This study analysed and compared the role of HIV-1 Nef-specific CD8 T-cell responses in patients with different disease status. Methods Two groups of patients with HIV-1 subtype B infection were selected according to CD4 count and clinical manifestations： long-term nonprogresssors （LTNPs, n = 20） and advanced progressors （APs, CD4 count 〈500 cells/pl, n = 34）. Nef-specific CD8 T-cell responses were studied by interferon- T ELISpot assay against 3 pools of HIV-Nef peptides. Results Nef-specific CD8 T-cell responses did not correlate with viral load or CD4 count in all patients and no significant differences were found in the magnitude of Nef-specific CD8 T-cell responses between groups LTNPs and APs （670 SFC/106 peripheral blood mononuclear cells vs 1107 SFC/106 peripheral blood mononuclear cells, P = 0.255）. Further comparisons showed that there were also no significant correlations observed in group LTNPs, but Nef-specific CD8 T cells correlated negatively with viral load （r = -0.397, P = 0.020） and positively with CD4 count （r = 0.364, P = 0.034） in group APs. Conclusion These data suggest that different correlation patterns between Nef-specific CD8 T-cell responses and disease progression exist in LTNPs and APs. Although a negative association was observed with concurrent plasma HIV RNA in APs, Nef-specific CD8 T-cell responses might fail to play a protective role in different stages of HIV- 1 infection.
