AIM: To explore the role and significance of costimulatory molecules B7H1,B7H2 and ICOS within tissues of human gastric carcinoma and the possible mechanisms in tumor escape.
METHODS: mRNA expressions of costimulatory...AIM: To explore the role and significance of costimulatory molecules B7H1,B7H2 and ICOS within tissues of human gastric carcinoma and the possible mechanisms in tumor escape.
METHODS: mRNA expressions of costimulatory molecules including B7H1,B7H2,ICOS and B7-1 in tissues of human gastric carcinoma were investigated by in situ hybridization using digoxigenin-labeled oligonucleotide-probes. The tissue of chronic gastric ulcer was used as a control. All data were analyzed by SPSS statistic software.
RESULTS: At the site of gastric carcinoma, mRNA expression levels of B7H1, B7H2 and ICOS were much higher than that of B7-1. Their mRNA positive expression indexes were 0.512±0.333, 0.812±0.454, 0.702±0.359 and 0.293±0.253, respectively. The positively stained cells were mainly tumor infiltrating lymphocytes (TILs), and some tumor cells. The difference between them was greatly significant P＜0.005. The mRNA expression levels of four molecules were not correlated to the pathological grade and matastasis of gastric carcinoma.
CONCLUSION: ICOS-B7H costimulatory pathway may be predominant at the site of gastric carcinoma. B7-1mRNA might be the basis of ICOS-B7H interaction. ICOS-B7H interaction induces the production of IL-10 which inhibits the antitumor immune responses. Therefore, it is supposed that ICOS-B7H costimulatory pathway may be involved in the negative regulation of cell-mediated immune responses.展开更多
AIM： To observe the status of tumor-associated B7 molecule mRNA expression in human colorectal cancer tissue by/n situ hybridization. METHODS： The mRNA expression patterns of cancer- associated B7-1,B7H1t,B7H2, ICOS...AIM： To observe the status of tumor-associated B7 molecule mRNA expression in human colorectal cancer tissue by/n situ hybridization. METHODS： The mRNA expression patterns of cancer- associated B7-1,B7H1t,B7H2, ICOS in 22 specimens of human colorectal cancer tissue were monitored by in situ hybridization （ISH） with digoxin-labeled oligonucleotide probes. RESULTS： B7-1, B7H1,B7H2,ICOS mRNA were detected in both cancer cells and tumor infiltrating lymphocytes （TIL）. The mRNA expression level of these molecules in tumor cells was higher than that in TIL （0.76±0.54-1.62±0.82 vs 0.38±0.19-0.65±0.33, P〈0.001）. There was no relationship between expression level of tested B7 family molecules and patients＇ sex, age, differentiation status of cancer and regional lymph node metastasis. CONCLUSION： Th2 cytokine predominant in tumor microenvironment might be related to the expression of B7H1t B7H2 co-signal molecules in tumor cells and TIL. 2005 The WJG Press and Elsevier Inc. All rights reserved展开更多
Objectives:A study to determine food safety hazard status of fish products from Lake Chivero was conducted in selected high density suburbs of Harare.Lake water and fish were tested for E.coli O157:H7,total bacterial,...Objectives:A study to determine food safety hazard status of fish products from Lake Chivero was conducted in selected high density suburbs of Harare.Lake water and fish were tested for E.coli O157:H7,total bacterial,fungal counts,mercury(Hg)and aflatoxin B1(AFB1)to determine contamination levels and assessing human health hazard exposure.Materials and Methods:Membrane filtration method was used to determine E.coli O157:H7 viable counts using CHROM agar.Plate count and Potato dextrose agar were used for determination of total viable bacterial and fungal counts.Concentrations of Hg and AFB1 in fish and water were determined by Atomic Absorption Spectrophotometer and High Performance Liquid Chromatography-Mass Spectrometry.A questionnaire survey was conducted on 136 adult fish consumers to determine the fish consumption patterns to assess hazard exposure against international standards.Results:Significant levels of microbial contamination above international standards in both fish and water were recorded.Mean E.coli O157:H7 counts were 106±10(cfu/cm^(2))in fish and 52±14(cfu/100ml)in water.Mean Log10 TBC were 8.98±0.26(cfu/cm^(2))in fish and 9.05±0.05(cfu/ml)in water.Mean Log10 TFC were 4.83±0.02(cfu/cm^(2))in fish and 4.56±0.03(cfu/ml)in water.Hg and AFB1 were 0.018(μg/kg)and 0.025(μg/kg)in fish and 0.008(μg/kg)and 0.005(μg/kg)in water,both with a hazard quotient(HQ)<1 using CODEX STAN 193-1995 of 2015.Conclusion:Fish and water are contaminated with E.coli O157:H7,contain bacterial and fungal loads above international food safety guidance levels.Fish were caught already contaminated with pathogens together with toxigenic Hg and carcinogenic AFB1 although their concentration levels are within international food safety toxicological levels.Levels of Hg and AFB1 in water were almost double that of fish,implying bio-accumulation occurred in fish.Thus,fish consumers are exposed to food safety hazards and are at risk of contracting foodborne illnesses from consuming contaminated fish.展开更多
文摘AIM: To explore the role and significance of costimulatory molecules B7H1,B7H2 and ICOS within tissues of human gastric carcinoma and the possible mechanisms in tumor escape.
METHODS: mRNA expressions of costimulatory molecules including B7H1,B7H2,ICOS and B7-1 in tissues of human gastric carcinoma were investigated by in situ hybridization using digoxigenin-labeled oligonucleotide-probes. The tissue of chronic gastric ulcer was used as a control. All data were analyzed by SPSS statistic software.
RESULTS: At the site of gastric carcinoma, mRNA expression levels of B7H1, B7H2 and ICOS were much higher than that of B7-1. Their mRNA positive expression indexes were 0.512±0.333, 0.812±0.454, 0.702±0.359 and 0.293±0.253, respectively. The positively stained cells were mainly tumor infiltrating lymphocytes (TILs), and some tumor cells. The difference between them was greatly significant P＜0.005. The mRNA expression levels of four molecules were not correlated to the pathological grade and matastasis of gastric carcinoma.
CONCLUSION: ICOS-B7H costimulatory pathway may be predominant at the site of gastric carcinoma. B7-1mRNA might be the basis of ICOS-B7H interaction. ICOS-B7H interaction induces the production of IL-10 which inhibits the antitumor immune responses. Therefore, it is supposed that ICOS-B7H costimulatory pathway may be involved in the negative regulation of cell-mediated immune responses.
基金Supported by the National Natural Science Foundation of China, No. 30370549
文摘AIM： To observe the status of tumor-associated B7 molecule mRNA expression in human colorectal cancer tissue by/n situ hybridization. METHODS： The mRNA expression patterns of cancer- associated B7-1,B7H1t,B7H2, ICOS in 22 specimens of human colorectal cancer tissue were monitored by in situ hybridization （ISH） with digoxin-labeled oligonucleotide probes. RESULTS： B7-1, B7H1,B7H2,ICOS mRNA were detected in both cancer cells and tumor infiltrating lymphocytes （TIL）. The mRNA expression level of these molecules in tumor cells was higher than that in TIL （0.76±0.54-1.62±0.82 vs 0.38±0.19-0.65±0.33, P〈0.001）. There was no relationship between expression level of tested B7 family molecules and patients＇ sex, age, differentiation status of cancer and regional lymph node metastasis. CONCLUSION： Th2 cytokine predominant in tumor microenvironment might be related to the expression of B7H1t B7H2 co-signal molecules in tumor cells and TIL. 2005 The WJG Press and Elsevier Inc. All rights reserved
文摘Objectives:A study to determine food safety hazard status of fish products from Lake Chivero was conducted in selected high density suburbs of Harare.Lake water and fish were tested for E.coli O157:H7,total bacterial,fungal counts,mercury(Hg)and aflatoxin B1(AFB1)to determine contamination levels and assessing human health hazard exposure.Materials and Methods:Membrane filtration method was used to determine E.coli O157:H7 viable counts using CHROM agar.Plate count and Potato dextrose agar were used for determination of total viable bacterial and fungal counts.Concentrations of Hg and AFB1 in fish and water were determined by Atomic Absorption Spectrophotometer and High Performance Liquid Chromatography-Mass Spectrometry.A questionnaire survey was conducted on 136 adult fish consumers to determine the fish consumption patterns to assess hazard exposure against international standards.Results:Significant levels of microbial contamination above international standards in both fish and water were recorded.Mean E.coli O157:H7 counts were 106±10(cfu/cm^(2))in fish and 52±14(cfu/100ml)in water.Mean Log10 TBC were 8.98±0.26(cfu/cm^(2))in fish and 9.05±0.05(cfu/ml)in water.Mean Log10 TFC were 4.83±0.02(cfu/cm^(2))in fish and 4.56±0.03(cfu/ml)in water.Hg and AFB1 were 0.018(μg/kg)and 0.025(μg/kg)in fish and 0.008(μg/kg)and 0.005(μg/kg)in water,both with a hazard quotient(HQ)<1 using CODEX STAN 193-1995 of 2015.Conclusion:Fish and water are contaminated with E.coli O157:H7,contain bacterial and fungal loads above international food safety guidance levels.Fish were caught already contaminated with pathogens together with toxigenic Hg and carcinogenic AFB1 although their concentration levels are within international food safety toxicological levels.Levels of Hg and AFB1 in water were almost double that of fish,implying bio-accumulation occurred in fish.Thus,fish consumers are exposed to food safety hazards and are at risk of contracting foodborne illnesses from consuming contaminated fish.
