The effects of BCG PSN on T cell subsets and cytokines in vernal conjunctivitis were observed. The level of total IgE was quantitatively determined before and after treatment with BCG PSN by allergen diagnostic ins...The effects of BCG PSN on T cell subsets and cytokines in vernal conjunctivitis were observed. The level of total IgE was quantitatively determined before and after treatment with BCG PSN by allergen diagnostic instrument in vitro . The content of T cell subsets of peripheral blood and cytokine were determined by using indirect immune fluorescence method, and IL 4 and INF γ were quantified by ELISA. The results showed that the level of total IgE was substantially reduced ( P <0.01) after treatment in the BCG PSN group. Meanwhile, CD + 8 was decreased, CD + 4 and CD + 4/CD + 8 ratio elevated with significant differences ( P <0.05) as compared with pre treatment results. The changes in total IgE, CD + 8 ,CD + 4 and CD + 4/CD + 8 ratio after treatment also presented significant differences ( P <0.05) between BCG PSN group and routine treatment group. The level of IL 4 in serum declined ( P <0.05) after treatment in the BCG PSN group, and INF γ went up ( P <0 05). IL 4 and INF γ in serum showed significant differences ( P <0.05) between two groups after treatment. It is concluded that BCG PSN has a bi directional immunoregulating effect. It can bring CD + 4 and CD + 8 into homeostasis, thereby preventing the occurrence of anaphylaxis. At the same time, BCG PSN can restrain Th 2, decrease the synthesis of IL 4, switch the balance of Th l/Th 2 to Th 1 side, boost up the predominance of Th 1 relatively, which is propitious to perennial stabilization and recovery of vernal conjunctivitis.展开更多
目的探究自血穴注疗法联合卡介苗多糖核酸治疗小儿支气管哮喘的临床疗效。方法选取庆阳市妇幼保健医院2020年1月—2022年1月收治的120例支气管哮喘患儿为研究对象,随机将其分为联合组和传统组各60例,给予传统组卡介苗多糖核酸治疗,联合...目的探究自血穴注疗法联合卡介苗多糖核酸治疗小儿支气管哮喘的临床疗效。方法选取庆阳市妇幼保健医院2020年1月—2022年1月收治的120例支气管哮喘患儿为研究对象,随机将其分为联合组和传统组各60例,给予传统组卡介苗多糖核酸治疗,联合组在传统组的基础上采用自血穴注疗法治疗,比较临床疗效,肺功能改善情况,日间症状积分,夜间症状积分以及生活质量积分变化,免疫功能相关指标水平变化及不良反应发生情况。结果联合组总有效率高于传统组(P<0.05);治疗后4周两组患儿第1秒用力呼气容积(forced expiratory volume in the first second,FEV1)、用力呼气流量(forced expiratory flow,FEF)水平均显著升高,且联合组显著高于传统组(P<0.05);日间症状积分和夜间症状积分均降低,生活质量积分升高,联合组显著优于传统组(P<0.05);治疗后CD_(3)^(+)、CD_(4)^(+)、CD_(4)^(+)/CD_(8)^(+)水平均显著升高,且联合组显著高于传统组(P<0.05);两组均未发生不良反应。结论自血穴注疗法联合卡介苗多糖核酸治疗小儿支气管哮喘安全高效,可广泛应用于临床。展开更多
文摘The effects of BCG PSN on T cell subsets and cytokines in vernal conjunctivitis were observed. The level of total IgE was quantitatively determined before and after treatment with BCG PSN by allergen diagnostic instrument in vitro . The content of T cell subsets of peripheral blood and cytokine were determined by using indirect immune fluorescence method, and IL 4 and INF γ were quantified by ELISA. The results showed that the level of total IgE was substantially reduced ( P <0.01) after treatment in the BCG PSN group. Meanwhile, CD + 8 was decreased, CD + 4 and CD + 4/CD + 8 ratio elevated with significant differences ( P <0.05) as compared with pre treatment results. The changes in total IgE, CD + 8 ,CD + 4 and CD + 4/CD + 8 ratio after treatment also presented significant differences ( P <0.05) between BCG PSN group and routine treatment group. The level of IL 4 in serum declined ( P <0.05) after treatment in the BCG PSN group, and INF γ went up ( P <0 05). IL 4 and INF γ in serum showed significant differences ( P <0.05) between two groups after treatment. It is concluded that BCG PSN has a bi directional immunoregulating effect. It can bring CD + 4 and CD + 8 into homeostasis, thereby preventing the occurrence of anaphylaxis. At the same time, BCG PSN can restrain Th 2, decrease the synthesis of IL 4, switch the balance of Th l/Th 2 to Th 1 side, boost up the predominance of Th 1 relatively, which is propitious to perennial stabilization and recovery of vernal conjunctivitis.
文摘目的探究自血穴注疗法联合卡介苗多糖核酸治疗小儿支气管哮喘的临床疗效。方法选取庆阳市妇幼保健医院2020年1月—2022年1月收治的120例支气管哮喘患儿为研究对象,随机将其分为联合组和传统组各60例,给予传统组卡介苗多糖核酸治疗,联合组在传统组的基础上采用自血穴注疗法治疗,比较临床疗效,肺功能改善情况,日间症状积分,夜间症状积分以及生活质量积分变化,免疫功能相关指标水平变化及不良反应发生情况。结果联合组总有效率高于传统组(P<0.05);治疗后4周两组患儿第1秒用力呼气容积(forced expiratory volume in the first second,FEV1)、用力呼气流量(forced expiratory flow,FEF)水平均显著升高,且联合组显著高于传统组(P<0.05);日间症状积分和夜间症状积分均降低,生活质量积分升高,联合组显著优于传统组(P<0.05);治疗后CD_(3)^(+)、CD_(4)^(+)、CD_(4)^(+)/CD_(8)^(+)水平均显著升高,且联合组显著高于传统组(P<0.05);两组均未发生不良反应。结论自血穴注疗法联合卡介苗多糖核酸治疗小儿支气管哮喘安全高效,可广泛应用于临床。
