Various biological indices were used to observe the effects of Tween-80 in combination with hyperthermia at different temperature (39-43℃) for different period of time (20-100 minutes) on human stomach cell line BGC-...Various biological indices were used to observe the effects of Tween-80 in combination with hyperthermia at different temperature (39-43℃) for different period of time (20-100 minutes) on human stomach cell line BGC-823. The results showed that Tween-80obviously reduced the activation energy of BGC-823 cells. Synergistic effect was observed if applied with heat at 39℃ with the increase in temperature and time,the inhibitory effect on the cancer cells was gradually intensified. The lethal rate of BGC-823 cells treated by heat at 41℃ in combination with Tween-80 was around 5.2 times as that treated by hyperthermia alone. The synergistic effect of heat at 41℃ for 100 min. in combination with Tween-80 was equivalent to the effect of 43℃ for 100 min.In other words, the critical temperature for BGC-823 cells was hereby reduced about 2℃. The measurement of membrane mobility, SDH activity etc.also showed that at 41℃ the synergistic effect of hyperthermia in combination with Tween-80 was the best, it exceeding the single effect of these factors in combination and showing effect of multiplication. The synergistic effect of heat at 41℃ in combination with Tween-80 was higher than that of heat at 41℃ in combination with MMC. It also demonstrated that the specific and sustained action on the inhibition of cancer cells did exist. These studies suggested that the synergistic mechanism of Tween-80 and hyperthermia probably was that both of them acted on the cell membrane system. Supported by The National Natural Science Fund of China (No. 3860948)展开更多
背景与目的：探讨丹参酮ⅡA（tanshinoneⅡA，TanⅡA）对人胃癌BGC-823细胞增殖及凋亡的影响。材料与方法：采用0～10μg／ml Tan ⅡA分别作用于BGC-823细胞48h及72h，MTT比色法观察药物对细胞生长的抑制效应；2、5、10μg／ml TanⅡA...背景与目的：探讨丹参酮ⅡA（tanshinoneⅡA，TanⅡA）对人胃癌BGC-823细胞增殖及凋亡的影响。材料与方法：采用0～10μg／ml Tan ⅡA分别作用于BGC-823细胞48h及72h，MTT比色法观察药物对细胞生长的抑制效应；2、5、10μg／ml TanⅡA分别作用于细胞72h，应用HE染色、DNA琼脂糖凝胶电泳、流式细胞术分析药物对细胞凋亡的影响。结果：TanⅡA明显抑制BGC-823细胞增殖、诱导细胞凋亡，镜下可见BGC-823细胞明显的凋亡特征性改变；5、10μg／ml Tan ⅡA处理组细胞DNA电泳可见典型的“梯状”条带；FCM结果显示5、10μg／ml TanⅡA处理组细胞，凋亡率分别为（20．60±1．84）％、（31．03±1．47）％，与对照组（5．23±0．39）％比较差异有统计学意义（P〈0．01）。结论：在一定条件下，TanⅡA可抑制人胃癌BGC-823细胞增殖，诱导其凋亡。展开更多
文摘Various biological indices were used to observe the effects of Tween-80 in combination with hyperthermia at different temperature (39-43℃) for different period of time (20-100 minutes) on human stomach cell line BGC-823. The results showed that Tween-80obviously reduced the activation energy of BGC-823 cells. Synergistic effect was observed if applied with heat at 39℃ with the increase in temperature and time,the inhibitory effect on the cancer cells was gradually intensified. The lethal rate of BGC-823 cells treated by heat at 41℃ in combination with Tween-80 was around 5.2 times as that treated by hyperthermia alone. The synergistic effect of heat at 41℃ for 100 min. in combination with Tween-80 was equivalent to the effect of 43℃ for 100 min.In other words, the critical temperature for BGC-823 cells was hereby reduced about 2℃. The measurement of membrane mobility, SDH activity etc.also showed that at 41℃ the synergistic effect of hyperthermia in combination with Tween-80 was the best, it exceeding the single effect of these factors in combination and showing effect of multiplication. The synergistic effect of heat at 41℃ in combination with Tween-80 was higher than that of heat at 41℃ in combination with MMC. It also demonstrated that the specific and sustained action on the inhibition of cancer cells did exist. These studies suggested that the synergistic mechanism of Tween-80 and hyperthermia probably was that both of them acted on the cell membrane system. Supported by The National Natural Science Fund of China (No. 3860948)
文摘背景与目的：探讨丹参酮ⅡA（tanshinoneⅡA，TanⅡA）对人胃癌BGC-823细胞增殖及凋亡的影响。材料与方法：采用0～10μg／ml Tan ⅡA分别作用于BGC-823细胞48h及72h，MTT比色法观察药物对细胞生长的抑制效应；2、5、10μg／ml TanⅡA分别作用于细胞72h，应用HE染色、DNA琼脂糖凝胶电泳、流式细胞术分析药物对细胞凋亡的影响。结果：TanⅡA明显抑制BGC-823细胞增殖、诱导细胞凋亡，镜下可见BGC-823细胞明显的凋亡特征性改变；5、10μg／ml Tan ⅡA处理组细胞DNA电泳可见典型的“梯状”条带；FCM结果显示5、10μg／ml TanⅡA处理组细胞，凋亡率分别为（20．60±1．84）％、（31．03±1．47）％，与对照组（5．23±0．39）％比较差异有统计学意义（P〈0．01）。结论：在一定条件下，TanⅡA可抑制人胃癌BGC-823细胞增殖，诱导其凋亡。
