目的探讨BIOMED-2标准化基因重排克隆性分析系统对原发性中枢神经系统淋巴瘤(primary central nervous system lymphoma,PCNSL)的检出率及应用价值。方法采用BIOMED-2系统引物对29例PCNSL、10例淋巴结反应性增生病变,进行Ig/TCR基因重...目的探讨BIOMED-2标准化基因重排克隆性分析系统对原发性中枢神经系统淋巴瘤(primary central nervous system lymphoma,PCNSL)的检出率及应用价值。方法采用BIOMED-2系统引物对29例PCNSL、10例淋巴结反应性增生病变,进行Ig/TCR基因重排的克隆性分析。结果 29例PCNSL中,BIOMED-2系统引物组合检测出27例克隆性重排,其中Ig克隆性重排26例(B细胞性淋巴瘤),另1例检测出TCR克隆性重排(T细胞性淋巴瘤),检出率为93.1%,10例淋巴结反应性增生病变均未见克隆性重排,其检测特异性为100%。结论 PCNSL是一种少见的高侵袭性结外非霍奇金淋巴瘤,利用BIOMED-2系统的Ig/TCR基因重排分析系统可对PCNSL的定性及分类提供客观性手段。展开更多
Broadcasting is a basic technique in Mobile ad-hoc network(MANET),and it refers to sending a packet from one node to every other node within the transmission range.Flooding is a type of broadcast where the received pa...Broadcasting is a basic technique in Mobile ad-hoc network(MANET),and it refers to sending a packet from one node to every other node within the transmission range.Flooding is a type of broadcast where the received packet is retransmitted once by every node.The naive flooding technique,floods the network with query messages,while the random walk technique operates by contacting the subsets of every node’s neighbors at each step,thereby restricting the search space.One of the key challenges in an ad-hoc network is the resource or content discovery problem which is about locating the queried resource.Many earlier works have mainly focused on the simulation-based analysis of flooding,and its variants under a wired network.Although,there have been some empirical studies in peer-to-peer(P2P)networks,the analytical results are still lacking,especially in the context of P2P systems running over MANET.In this paper,we describe how P2P resource discovery protocols perform badly over MANETs.To address the limitations,we propose a new protocol named ABRW(Address Broadcast Random Walk),which is a lightweight search approach,designed considering the underlay topology aimed to better suit the unstructured architecture.We provide the mathematical model,measuring the performance of our proposed search scheme with different widely popular benchmarked search techniques.Further,we also derive three relevant search performance metrics,i.e.,mean no.of steps needed to find a resource,the probability of finding a resource,and the mean no.of message overhead.We validated the analytical expressions through simulations.The simulation results closely matched with our analyticalmodel,justifying our findings.Our proposed search algorithm under such highly dynamic self-evolving networks performed better,as it reduced the search latency,decreased the overall message overhead,and still equally had a good success rate.展开更多
Most of the public key algorithms used in the exchange of information for power data transmission protocols are RSA. The core of the key part of this kind of algorithm system has not been announced. For the domestic s...Most of the public key algorithms used in the exchange of information for power data transmission protocols are RSA. The core of the key part of this kind of algorithm system has not been announced. For the domestic sensitive information data field, there are threats such as preset backdoors and security vulnerabilities. In response to the above problems, the article introduces a secure communication protocol based on the optimized Secret SM2 algorithm, which uses socket programming to achieve two-way encrypted communication between clients and services, and is able to complete the security protection of data encryption transmission, authentication, data tampering, etc., and proves through experiments that the security protocol is more secure than traditional methods, can effectively identify each other, carry out stable and controllable data encryption transmission, and has good applicability.展开更多
The electronic system in a comprehensive shipboard has a extremely wide bandwidth. It supports synchronized and asynchronous data transmission, muhimedia correspondence, and videophone conferencing. In order to guaran...The electronic system in a comprehensive shipboard has a extremely wide bandwidth. It supports synchronized and asynchronous data transmission, muhimedia correspondence, and videophone conferencing. In order to guarantee the security and correspondent real time of the communication, choosing a multicast routing protocol based on different applications is a key problem in terms of the complexity of the system. A simulation model was first designed for existing multicast routing protocols in NS-2 and analysis was performed on their corresponding application environments. The experiments proved that PIM-DM based on the shortest-path tree protocol is suitable for communication in the field of comprehensive shipboard. Further work that is necessary is also discussed.展开更多
The IEC60870-5-104 protocol lacks an integrated authentication mechanism during plaintext transmission, and is vulnerable to security threats, monitoring, tampering, or cutting off communication connections. In order ...The IEC60870-5-104 protocol lacks an integrated authentication mechanism during plaintext transmission, and is vulnerable to security threats, monitoring, tampering, or cutting off communication connections. In order to verify the security problems of 104 protocol, the 104 master-slave communication implemented DoS attacks, ARP spoofing and Ettercap packet filtering and other man-in-the-middle attacks. DoS attacks may damage the network functions of the 104 communication host, resulting in communication interruption. ARP spoofing damaged the data privacy of the 104 protocol, and Ettercap packet filtering cut off the communication connection between the master and the slave. In order to resist the man-in-the-middle attack, the AES and RSA hybrid encryption signature algorithm and the national secret SM2 elliptic curve algorithm are proposed. AES and RSA hybrid encryption increases the security strength of communication data and realizes identity authentication. The digital signature implemented by the SM2 algorithm can realize identity verification, ensure that the data has not been tampered with, and can ensure the integrity of the data. Both of them improve the communication security of the 104 protocol.展开更多
3D （Three-dimensional） Caco-2 spheroids closely recapitulating in vivo physiological organization of intestinal epithelial cells, provide an excellent in vitro model system to study their pathophysiology and their r...3D （Three-dimensional） Caco-2 spheroids closely recapitulating in vivo physiological organization of intestinal epithelial cells, provide an excellent in vitro model system to study their pathophysiology and their response to stressful stimuli. The objective of this technical note is to provide optimized in vitro experimental protocols for culturing 3D Caco-2 spheroids and for analyzing their cell growth features. An optimized 3D Caco-2 spheroid culturing technique based on a new configuration of the culture medium is provided A methodological approach to determine the distribution of the cell cycle phases in disaggregated Caco-2 spheroids by using cytofluorimetric analysis is also described. The optimized culturing protocol favors 3D Caco-2 spheroid differentiation process, as evaluated by the number of well-differentiated spheroids with a single hollow lumen. The cytofluorimetric analysis allows rapid collection of cell cycle phase data from high numbers of spheroid samples, thus, permitting to estimate their growth dynamics in a relatively short time. The optimized technical approaches described here can be applied in systematic manner to a variety of research activities utilizing 3D Caco-2 spheroids. Ease of use, time and economic saving advantages deriving from these protocols further highlight their potential.展开更多
文摘目的探讨BIOMED-2标准化基因重排克隆性分析系统对原发性中枢神经系统淋巴瘤(primary central nervous system lymphoma,PCNSL)的检出率及应用价值。方法采用BIOMED-2系统引物对29例PCNSL、10例淋巴结反应性增生病变,进行Ig/TCR基因重排的克隆性分析。结果 29例PCNSL中,BIOMED-2系统引物组合检测出27例克隆性重排,其中Ig克隆性重排26例(B细胞性淋巴瘤),另1例检测出TCR克隆性重排(T细胞性淋巴瘤),检出率为93.1%,10例淋巴结反应性增生病变均未见克隆性重排,其检测特异性为100%。结论 PCNSL是一种少见的高侵袭性结外非霍奇金淋巴瘤,利用BIOMED-2系统的Ig/TCR基因重排分析系统可对PCNSL的定性及分类提供客观性手段。
文摘Broadcasting is a basic technique in Mobile ad-hoc network(MANET),and it refers to sending a packet from one node to every other node within the transmission range.Flooding is a type of broadcast where the received packet is retransmitted once by every node.The naive flooding technique,floods the network with query messages,while the random walk technique operates by contacting the subsets of every node’s neighbors at each step,thereby restricting the search space.One of the key challenges in an ad-hoc network is the resource or content discovery problem which is about locating the queried resource.Many earlier works have mainly focused on the simulation-based analysis of flooding,and its variants under a wired network.Although,there have been some empirical studies in peer-to-peer(P2P)networks,the analytical results are still lacking,especially in the context of P2P systems running over MANET.In this paper,we describe how P2P resource discovery protocols perform badly over MANETs.To address the limitations,we propose a new protocol named ABRW(Address Broadcast Random Walk),which is a lightweight search approach,designed considering the underlay topology aimed to better suit the unstructured architecture.We provide the mathematical model,measuring the performance of our proposed search scheme with different widely popular benchmarked search techniques.Further,we also derive three relevant search performance metrics,i.e.,mean no.of steps needed to find a resource,the probability of finding a resource,and the mean no.of message overhead.We validated the analytical expressions through simulations.The simulation results closely matched with our analyticalmodel,justifying our findings.Our proposed search algorithm under such highly dynamic self-evolving networks performed better,as it reduced the search latency,decreased the overall message overhead,and still equally had a good success rate.
文摘Most of the public key algorithms used in the exchange of information for power data transmission protocols are RSA. The core of the key part of this kind of algorithm system has not been announced. For the domestic sensitive information data field, there are threats such as preset backdoors and security vulnerabilities. In response to the above problems, the article introduces a secure communication protocol based on the optimized Secret SM2 algorithm, which uses socket programming to achieve two-way encrypted communication between clients and services, and is able to complete the security protection of data encryption transmission, authentication, data tampering, etc., and proves through experiments that the security protocol is more secure than traditional methods, can effectively identify each other, carry out stable and controllable data encryption transmission, and has good applicability.
基金Supported by the Performance Simulation and Computation Complexity on Computer Network Foundation (No.F2004060)
文摘The electronic system in a comprehensive shipboard has a extremely wide bandwidth. It supports synchronized and asynchronous data transmission, muhimedia correspondence, and videophone conferencing. In order to guarantee the security and correspondent real time of the communication, choosing a multicast routing protocol based on different applications is a key problem in terms of the complexity of the system. A simulation model was first designed for existing multicast routing protocols in NS-2 and analysis was performed on their corresponding application environments. The experiments proved that PIM-DM based on the shortest-path tree protocol is suitable for communication in the field of comprehensive shipboard. Further work that is necessary is also discussed.
文摘The IEC60870-5-104 protocol lacks an integrated authentication mechanism during plaintext transmission, and is vulnerable to security threats, monitoring, tampering, or cutting off communication connections. In order to verify the security problems of 104 protocol, the 104 master-slave communication implemented DoS attacks, ARP spoofing and Ettercap packet filtering and other man-in-the-middle attacks. DoS attacks may damage the network functions of the 104 communication host, resulting in communication interruption. ARP spoofing damaged the data privacy of the 104 protocol, and Ettercap packet filtering cut off the communication connection between the master and the slave. In order to resist the man-in-the-middle attack, the AES and RSA hybrid encryption signature algorithm and the national secret SM2 elliptic curve algorithm are proposed. AES and RSA hybrid encryption increases the security strength of communication data and realizes identity authentication. The digital signature implemented by the SM2 algorithm can realize identity verification, ensure that the data has not been tampered with, and can ensure the integrity of the data. Both of them improve the communication security of the 104 protocol.
文摘3D （Three-dimensional） Caco-2 spheroids closely recapitulating in vivo physiological organization of intestinal epithelial cells, provide an excellent in vitro model system to study their pathophysiology and their response to stressful stimuli. The objective of this technical note is to provide optimized in vitro experimental protocols for culturing 3D Caco-2 spheroids and for analyzing their cell growth features. An optimized 3D Caco-2 spheroid culturing technique based on a new configuration of the culture medium is provided A methodological approach to determine the distribution of the cell cycle phases in disaggregated Caco-2 spheroids by using cytofluorimetric analysis is also described. The optimized culturing protocol favors 3D Caco-2 spheroid differentiation process, as evaluated by the number of well-differentiated spheroids with a single hollow lumen. The cytofluorimetric analysis allows rapid collection of cell cycle phase data from high numbers of spheroid samples, thus, permitting to estimate their growth dynamics in a relatively short time. The optimized technical approaches described here can be applied in systematic manner to a variety of research activities utilizing 3D Caco-2 spheroids. Ease of use, time and economic saving advantages deriving from these protocols further highlight their potential.
