With the continuous emergence and rapid spread of multidrug-resistant and extensively-drug-resistant Mycobacterium tuberculosis strains, it is imperative to develop novel therapies against this bacterium. The intrins...With the continuous emergence and rapid spread of multidrug-resistant and extensively-drug-resistant Mycobacterium tuberculosis strains, it is imperative to develop novel therapies against this bacterium. The intrinsic β-lactam resistance of M. tuberculosis is primarily due to the production of an Ambler class-A β-lactamase BlaC, which limits the application of β-lactam antibiotics in the treatment of tuberculosis. Therefore, the inhibitors of BlaC could be novel anti-tuberculosis drug synergistic agents to recover the sensibility of M. Tuberculosis to the β-lactam antibiotics. In the present study, BlaC of M. tuberculosis was expressed and purified to establish a screening model of the BlaC inhibitors. The screening conditions were determined, and the screening model was evaluated to fit for the high throughput screening. A total of 22 BlaC inhibitors were screened out from 26 400 compound samples with a positive rate of 0.083%. Taken together, our findings lay the foundation for the discovery of novel anti-tuberculosis drug synergistic agents in clinic.展开更多
本文介绍了一种基于离散事件系统(DES)的无刷交流(BLAC)电机的电流控制系统,提出系统的控制器带有图形用户界面(GUI),能够直接显示出有限状态机(FSM)的状态。通过应用FPGA对原有DES控制系统进行改进,将这五个选定的矢量视为的DES离散状...本文介绍了一种基于离散事件系统(DES)的无刷交流(BLAC)电机的电流控制系统,提出系统的控制器带有图形用户界面(GUI),能够直接显示出有限状态机(FSM)的状态。通过应用FPGA对原有DES控制系统进行改进,将这五个选定的矢量视为的DES离散状态,合理设置FSM选择矢量规则,实现了限定所有可能的方向。在Xilinx Spartan 3E FPGA平台进行的硬件测试结果显示,本文提出的方法减少了60%以上的开关变化数量和开关损耗,证明了提出的DES改进方法对负载变化具有稳定性。展开更多
基金Fundamental Research Funds for Central Public Welfare Research Institutes(Grant No.2015PT350001)National Major Scientific and Technological Special Project for “Significant New Drugs Development”(Grant No.2015ZX09102007-009)
文摘With the continuous emergence and rapid spread of multidrug-resistant and extensively-drug-resistant Mycobacterium tuberculosis strains, it is imperative to develop novel therapies against this bacterium. The intrinsic β-lactam resistance of M. tuberculosis is primarily due to the production of an Ambler class-A β-lactamase BlaC, which limits the application of β-lactam antibiotics in the treatment of tuberculosis. Therefore, the inhibitors of BlaC could be novel anti-tuberculosis drug synergistic agents to recover the sensibility of M. Tuberculosis to the β-lactam antibiotics. In the present study, BlaC of M. tuberculosis was expressed and purified to establish a screening model of the BlaC inhibitors. The screening conditions were determined, and the screening model was evaluated to fit for the high throughput screening. A total of 22 BlaC inhibitors were screened out from 26 400 compound samples with a positive rate of 0.083%. Taken together, our findings lay the foundation for the discovery of novel anti-tuberculosis drug synergistic agents in clinic.
文摘本文介绍了一种基于离散事件系统(DES)的无刷交流(BLAC)电机的电流控制系统,提出系统的控制器带有图形用户界面(GUI),能够直接显示出有限状态机(FSM)的状态。通过应用FPGA对原有DES控制系统进行改进,将这五个选定的矢量视为的DES离散状态,合理设置FSM选择矢量规则,实现了限定所有可能的方向。在Xilinx Spartan 3E FPGA平台进行的硬件测试结果显示,本文提出的方法减少了60%以上的开关变化数量和开关损耗,证明了提出的DES改进方法对负载变化具有稳定性。