目的:研究脂氧素类似物BML-111对脂多糖(lipopolysaccharide,LPS)诱导的宫颈癌细胞株Hela细胞增殖及骨桥蛋白(osteopontin,OPN)表达的影响。方法:分别用100、10、1、0.1μg/m L LPS刺激Hela细胞,MTT方法检测其对细胞存活率的影响;Wester...目的:研究脂氧素类似物BML-111对脂多糖(lipopolysaccharide,LPS)诱导的宫颈癌细胞株Hela细胞增殖及骨桥蛋白(osteopontin,OPN)表达的影响。方法:分别用100、10、1、0.1μg/m L LPS刺激Hela细胞,MTT方法检测其对细胞存活率的影响;Western blot免疫印迹法检测LPS对Hela细胞OPN及p53蛋白表达的影响。Hela细胞分4组:(1)空白组;(2)LPS组;(3)LPS+BML-111组;(4)LPS+BML-111+Boc-2(BML-111受体FPR2/ALX的阻断剂)组。MTT方法检测BML-111对LPS诱导的Hela细胞存活率的影响;Western blot免疫印迹法检测BML-111对LPS诱导的Hela细胞OPN及p53(增殖指标)蛋白表达的影响。结果:与对照组相比,100、10、1、0.1μg/m L LPS均能明显促进Hela增殖及OPN、p53蛋白表达(P<0.05),但不同浓度组间比较差异无显著性(P>0.05)。BML-111能明显抑制LPS诱导的Hela细胞存活率,并能抑制OPN及p53蛋白的表达,Boc-2能阻断这种效应。结论:BML-111能抑制LPS诱导Hela细胞增殖,其作用可能是通过与受体结合,进而抑制OPN下调p53实现。展开更多
Objective:To investigate the effect of lipoxin receptor agonist BML-111 on the NLRP3 inflammasome after traumatic brain injury in rats.Methods:Sixty male Sprague-Dawley rats,weighing 280~340 g,were randomly divided in...Objective:To investigate the effect of lipoxin receptor agonist BML-111 on the NLRP3 inflammasome after traumatic brain injury in rats.Methods:Sixty male Sprague-Dawley rats,weighing 280~340 g,were randomly divided into 4 groups(n=15):the sham operation group(group Sham),the traumatic brain injury group(group TBI),the BML-111 treatment group(group BML-111),and the BOC-2 treatment group(group BOC-2).The TBI model was prepared by craniocerebral collision,while the rats in group Sham underwent only craniotomy without collision.Acute traumatic brain injury model was prepared in group TBI,BML-111 and BOC-2.The rats in group BOC-2 were intraperitoneally injected with 50μg/kg of BOC-230 min prior to trauma.Then the rats in group BOC-2 and BML-111 were injected intraperitoneally with 1 mg/kg of BML-111 immediately and 24 hours after trauma.The neurological severity scores(NSS)were evaluated at 3 and 7 days after brain trauma.The protein expression levels of NLRP3,Caspase-1-p20 and active Caspase-3 were determined by Western blot.The content of IL-1βand IL-18 was detected by ELISA assays.The apoptotic cells were analyzed by the TUNEL method.Results:Compared with group Sham,the brain water content and NSS scores in group TBI were increased,and the protein levels of NLRP3,Caspase-1-p20,activated Caspase-3,IL-1βand IL-18 as well as TUNEL-positive cells in the cortex were elevated significantly(P<0.05);compared with group TBI,the brain water content and NSS scores in group BML-111 were reduced,and the protein levels of NLRP3,Caspase-1-p20,activated Caspase-3,IL-1βand IL-18 as well as TUNEL-positive cells in the cortex were decreased(P<0.05);Compared with group BML-111,the brain water content and NSS scores in group BOC-2 were increased,and the protein levels of NLRP3,Caspase-1-p20,activated Caspase-3,IL-1βand IL-18 as well as TUNEL-positive cells in the cortex were up-regulated(P<0.05).Conclusions:The lipoxin receptor agonist BML-111 might attenuate traumatic brain injury in rats by inhibiting NLRP3 inflammasome activation.展开更多
文摘目的:研究脂氧素类似物BML-111对脂多糖(lipopolysaccharide,LPS)诱导的宫颈癌细胞株Hela细胞增殖及骨桥蛋白(osteopontin,OPN)表达的影响。方法:分别用100、10、1、0.1μg/m L LPS刺激Hela细胞,MTT方法检测其对细胞存活率的影响;Western blot免疫印迹法检测LPS对Hela细胞OPN及p53蛋白表达的影响。Hela细胞分4组:(1)空白组;(2)LPS组;(3)LPS+BML-111组;(4)LPS+BML-111+Boc-2(BML-111受体FPR2/ALX的阻断剂)组。MTT方法检测BML-111对LPS诱导的Hela细胞存活率的影响;Western blot免疫印迹法检测BML-111对LPS诱导的Hela细胞OPN及p53(增殖指标)蛋白表达的影响。结果:与对照组相比,100、10、1、0.1μg/m L LPS均能明显促进Hela增殖及OPN、p53蛋白表达(P<0.05),但不同浓度组间比较差异无显著性(P>0.05)。BML-111能明显抑制LPS诱导的Hela细胞存活率,并能抑制OPN及p53蛋白的表达,Boc-2能阻断这种效应。结论:BML-111能抑制LPS诱导Hela细胞增殖,其作用可能是通过与受体结合,进而抑制OPN下调p53实现。
文摘Objective:To investigate the effect of lipoxin receptor agonist BML-111 on the NLRP3 inflammasome after traumatic brain injury in rats.Methods:Sixty male Sprague-Dawley rats,weighing 280~340 g,were randomly divided into 4 groups(n=15):the sham operation group(group Sham),the traumatic brain injury group(group TBI),the BML-111 treatment group(group BML-111),and the BOC-2 treatment group(group BOC-2).The TBI model was prepared by craniocerebral collision,while the rats in group Sham underwent only craniotomy without collision.Acute traumatic brain injury model was prepared in group TBI,BML-111 and BOC-2.The rats in group BOC-2 were intraperitoneally injected with 50μg/kg of BOC-230 min prior to trauma.Then the rats in group BOC-2 and BML-111 were injected intraperitoneally with 1 mg/kg of BML-111 immediately and 24 hours after trauma.The neurological severity scores(NSS)were evaluated at 3 and 7 days after brain trauma.The protein expression levels of NLRP3,Caspase-1-p20 and active Caspase-3 were determined by Western blot.The content of IL-1βand IL-18 was detected by ELISA assays.The apoptotic cells were analyzed by the TUNEL method.Results:Compared with group Sham,the brain water content and NSS scores in group TBI were increased,and the protein levels of NLRP3,Caspase-1-p20,activated Caspase-3,IL-1βand IL-18 as well as TUNEL-positive cells in the cortex were elevated significantly(P<0.05);compared with group TBI,the brain water content and NSS scores in group BML-111 were reduced,and the protein levels of NLRP3,Caspase-1-p20,activated Caspase-3,IL-1βand IL-18 as well as TUNEL-positive cells in the cortex were decreased(P<0.05);Compared with group BML-111,the brain water content and NSS scores in group BOC-2 were increased,and the protein levels of NLRP3,Caspase-1-p20,activated Caspase-3,IL-1βand IL-18 as well as TUNEL-positive cells in the cortex were up-regulated(P<0.05).Conclusions:The lipoxin receptor agonist BML-111 might attenuate traumatic brain injury in rats by inhibiting NLRP3 inflammasome activation.
