Conventionally available Polyvinyl acetate (PVAc) wood glues are polyvinyl alcohol (PVA) stabilized, with drawbacks like poor strength at high humidity, poor strength at high temperature and workability at low-tempera...Conventionally available Polyvinyl acetate (PVAc) wood glues are polyvinyl alcohol (PVA) stabilized, with drawbacks like poor strength at high humidity, poor strength at high temperature and workability at low-temperature. PVAc is non-resistant to high humidity, and if such adhesive bonds are exploited in a highly humid environment, its strength substantially decreases. Sufficiently water-resistant adhesive bonds are achieved by modifying PVAc dispersion with special chemicals like acrylic acid (AA) and N-methylol acrylamide (NMA) as a co-monomer, Silanes, and ethylene modified PVA. The Lewis acids like aluminium chloride and aluminium nitrate are used as cross-linkers. So PVAc adhesives are classified as reactive and non-reactive glue. Application of non-reactive D1 (as per EN 204-205) and reactive D2 and D3 (as per EN 204-205) adhesives for bonding laminate on plywood is a regular practice in the Indian market. In summer time, Crack formation was seen in laminate bonded with reactive D2 and D3 adhesives in regions where the room temperature was above 45°C. However, if the same laminate substrates were bonded with non-reactive D1, no cracks were seen. To analyse the above phenomenon, we have done Dynamic mechanical analysis of non-reactive D1, reactive D2 and D3 adhesive.展开更多
Dopamine （DA） exposure at a dose of 100 pmol/L for 24 hours causes oxidative stress in SH-SY5Y cells with induction of neuronal differentiation by retinoic acid （RA,10 pmol/L,72 hours） followed by phorbol ester 12...Dopamine （DA） exposure at a dose of 100 pmol/L for 24 hours causes oxidative stress in SH-SY5Y cells with induction of neuronal differentiation by retinoic acid （RA,10 pmol/L,72 hours） followed by phorbol ester 12-O-tetradecanoyl-phorbol-13-acetate （TPA,80 nmol/L,72 hours）. However,it remains unclear whether the alteration of phenotype observed in response to oxidative stress is associated with protein regulation in this cellular model for Parkinson＇s disease. The present study detected protein regulation affected by oxidative stress at a proteomic level：selection of differentially altered proteins using two dimensional difference in-gel electrophoresis and identification of these proteins using matrix assisted laser desorption/ionization time-of-flight mass spectrometry. The results demonstrated significant alterations in expression of six proteins in SH-SY5Y cells following the differentiation and fourteen proteins in the differentiated cells following the exposure,exemplified by an increase of tubulin alpha1 in the former but a decrease of tubulin alpha-ubiquitous chain in the latter. These results suggest that two potentially specific but relevant patterns of proteomic change may be produced in SH-SY5Y cells with the induction of differentiation by RA followed by TPA,and in the differentiated cells after DA exposure.展开更多
The fluorescence emission wavelength [lambda(max(em))] values of three types of benzaldehyde derivatives, namely, ethylene acetals (1-Ys), 4-nitrophenylhydrazones (2-Ys) and phenylhydrazones (3-Ys), have been measured...The fluorescence emission wavelength [lambda(max(em))] values of three types of benzaldehyde derivatives, namely, ethylene acetals (1-Ys), 4-nitrophenylhydrazones (2-Ys) and phenylhydrazones (3-Ys), have been measured. Correlation analyses by the dual-parameter equation show that the lambda(max(em)) values of 1-Ys are mainly affected by the spin-delocalization effects of the substituents, while those of 2-Ys are mainly affected by the polar effects. However, those of 3-Ys are independent of the substituents.展开更多
OBJECTIVE:To investigate the antitumor effects of bornyl acetate(BA)isolated from Sharen(Fructus Amomi)in colorectal cancer(CRC)and the underlying mechanisms.METHODS:SW480 and HT29 cells were treated with increasing d...OBJECTIVE:To investigate the antitumor effects of bornyl acetate(BA)isolated from Sharen(Fructus Amomi)in colorectal cancer(CRC)and the underlying mechanisms.METHODS:SW480 and HT29 cells were treated with increasing doses of BA in order to determine its antitumor effects in vitro.Cell viability,colony formation,cell cycle,and apoptosis as well as migration and invasion were assessed using various assays.In addition,the in vivo antitumor effects of BA were assessed using a xenograft mouse model.We then assessed the mechanism of action of BA by conducting pathway activator-mediated rescue experiments and assessed the protein levels by Western blot analysis.RESULTS:BA showed anti-CRC tumor activities in vitro by suppressing cell proliferation and colony formation,inducing apoptosis,blocking cell cycle,and inhibiting migration and invasion.These effects were mediated via suppression of the phosphatidylinositol-3-kinase/protein kinase B(PI3K/AKT)pathway.In the tumor xenograft experiment,BA was found to repress tumor growth in vivo with low toxicity.CONCLUSIONS:The results demonstrated that BA exerts antitumor effects by suppressing the PI3K/AKT pathway,with low toxicity.Thus,BA might be a potential novel therapeutic agent for CRC.展开更多
The aim of this study was to evaluate the effect of naringin on experimentally induced inflammatory bowel dis- ease in rats. Naringin （20, 40 and 80 mg/kg） was given orally for 7 days to Wistar rats before induction...The aim of this study was to evaluate the effect of naringin on experimentally induced inflammatory bowel dis- ease in rats. Naringin （20, 40 and 80 mg/kg） was given orally for 7 days to Wistar rats before induction of colitis by intrarectal instillation of 2 mL of 4% （v/v） acetic acid solution. The degree of colonic mucosal damage was analyzed by examining mucosal damage, ulcer area, ulcer index and stool consistency. Intrarectal administration of 4% acetic acid resulted in significant modulation of serum alkaline phosphatase, lactate dehydrogenase, superoxide dismutase （SOD）, glutathione （GSH）, malondialdehyde （MDA） and myeloperoxidase （MPO） content along with colonic nitric oxide （NO）, xanthine oxidase （XO） level and protein carbonyl content in the colonic tissue as well as in blood. Naringin （40 and 80 mg/kg） exerted a dose dependent （P 〈 0.05） ameliorative effect, as it significantly increased hematological parameter as well as colonic SOD and GSH. There was a significant （P 〈 0.05） and dose dependant inhibition of macroscopical score, ulcer area along with colonic MDA, MPO activity by the 7 days of pretreatment of naringin （40 and 80 mg/kg）. Biochemical studies revealed a significant （P 〈 0.05） dose dependant inhibition in serum alkaline phosphatase （ALP） and lactate dehydrogenase （LDH） levels by pretreatment of naringin. Increased levels of colonic NO, XO, protein carbonyl content and DNA damage were also sig- nificantly decreased by naringin pretreatment. The findings of the present investigation propose that naringin has an anti-inflammatory, anti-oxidant and anti-apoptotic potential effect at colorectal sites as it modulates the production and expression of oxidative mediators such as MDA, MPO, NO and XO, thus reducing DNA damage.展开更多
Some new complexes RE2(EDODA)3·3H2O, where RE = La, Nd, Eu, Gd, Tb, Er, Yb, Lu and Y, EDODA = ethylene-1,2-dioxydiacetate, have been synthesized and characterized by elemental analysis, molar conductance, IR...Some new complexes RE2(EDODA)3·3H2O, where RE = La, Nd, Eu, Gd, Tb, Er, Yb, Lu and Y, EDODA = ethylene-1,2-dioxydiacetate, have been synthesized and characterized by elemental analysis, molar conductance, IR spectra, UV spectra, TG-DTA, 1H NMR and 13C NMR spectra. Various analyses indicate that the complexes are of nine-coordinated binuclear structure. The carboxylates are bidentate ligands and the ether oxygen atoms also coordinate to rare earth ions. Three water molecules are crystalline water. In addition, the influence of concentration on the chemical shift has been studied through the 1H NMR spectra of the complex Lu2(EDODA)3·3H2O in different concentrations.展开更多
文摘Conventionally available Polyvinyl acetate (PVAc) wood glues are polyvinyl alcohol (PVA) stabilized, with drawbacks like poor strength at high humidity, poor strength at high temperature and workability at low-temperature. PVAc is non-resistant to high humidity, and if such adhesive bonds are exploited in a highly humid environment, its strength substantially decreases. Sufficiently water-resistant adhesive bonds are achieved by modifying PVAc dispersion with special chemicals like acrylic acid (AA) and N-methylol acrylamide (NMA) as a co-monomer, Silanes, and ethylene modified PVA. The Lewis acids like aluminium chloride and aluminium nitrate are used as cross-linkers. So PVAc adhesives are classified as reactive and non-reactive glue. Application of non-reactive D1 (as per EN 204-205) and reactive D2 and D3 (as per EN 204-205) adhesives for bonding laminate on plywood is a regular practice in the Indian market. In summer time, Crack formation was seen in laminate bonded with reactive D2 and D3 adhesives in regions where the room temperature was above 45°C. However, if the same laminate substrates were bonded with non-reactive D1, no cracks were seen. To analyse the above phenomenon, we have done Dynamic mechanical analysis of non-reactive D1, reactive D2 and D3 adhesive.
基金the Science and Technology Development Program of Jilin Province, No. 200505200the Distinguished Professor Foundation of Jilin University, No. 450011011204
文摘Dopamine （DA） exposure at a dose of 100 pmol/L for 24 hours causes oxidative stress in SH-SY5Y cells with induction of neuronal differentiation by retinoic acid （RA,10 pmol/L,72 hours） followed by phorbol ester 12-O-tetradecanoyl-phorbol-13-acetate （TPA,80 nmol/L,72 hours）. However,it remains unclear whether the alteration of phenotype observed in response to oxidative stress is associated with protein regulation in this cellular model for Parkinson＇s disease. The present study detected protein regulation affected by oxidative stress at a proteomic level：selection of differentially altered proteins using two dimensional difference in-gel electrophoresis and identification of these proteins using matrix assisted laser desorption/ionization time-of-flight mass spectrometry. The results demonstrated significant alterations in expression of six proteins in SH-SY5Y cells following the differentiation and fourteen proteins in the differentiated cells following the exposure,exemplified by an increase of tubulin alpha1 in the former but a decrease of tubulin alpha-ubiquitous chain in the latter. These results suggest that two potentially specific but relevant patterns of proteomic change may be produced in SH-SY5Y cells with the induction of differentiation by RA followed by TPA,and in the differentiated cells after DA exposure.
文摘The fluorescence emission wavelength [lambda(max(em))] values of three types of benzaldehyde derivatives, namely, ethylene acetals (1-Ys), 4-nitrophenylhydrazones (2-Ys) and phenylhydrazones (3-Ys), have been measured. Correlation analyses by the dual-parameter equation show that the lambda(max(em)) values of 1-Ys are mainly affected by the spin-delocalization effects of the substituents, while those of 2-Ys are mainly affected by the polar effects. However, those of 3-Ys are independent of the substituents.
基金National Key R&D Program of China:Underground Ecological Planting Technology and Base Establishment of Sharen (Fructus Amomi) in the forest (2017YFC1701102)West Yunnan University of Applied Sciences University-level Engineering Research Center projects:Characteristic Dai-Medicine Resource ERC of West Yunnan University of Apllied Science (2022KYPT0004)+3 种基金National Natural Science Foundation of China:Study on the symbiotic system of Sharen (Fructus Amomi)weevil pollination and its "push-pull" pollination mechanism (82260736)Yunnan key labotatory of southern medicine utilization:Major Science and Technology Special Plan of Yunnan Province (202102AA100020)Scientific and Technological Talents and Platform Plan of Yunnan Province (202105AG070011)
文摘OBJECTIVE:To investigate the antitumor effects of bornyl acetate(BA)isolated from Sharen(Fructus Amomi)in colorectal cancer(CRC)and the underlying mechanisms.METHODS:SW480 and HT29 cells were treated with increasing doses of BA in order to determine its antitumor effects in vitro.Cell viability,colony formation,cell cycle,and apoptosis as well as migration and invasion were assessed using various assays.In addition,the in vivo antitumor effects of BA were assessed using a xenograft mouse model.We then assessed the mechanism of action of BA by conducting pathway activator-mediated rescue experiments and assessed the protein levels by Western blot analysis.RESULTS:BA showed anti-CRC tumor activities in vitro by suppressing cell proliferation and colony formation,inducing apoptosis,blocking cell cycle,and inhibiting migration and invasion.These effects were mediated via suppression of the phosphatidylinositol-3-kinase/protein kinase B(PI3K/AKT)pathway.In the tumor xenograft experiment,BA was found to repress tumor growth in vivo with low toxicity.CONCLUSIONS:The results demonstrated that BA exerts antitumor effects by suppressing the PI3K/AKT pathway,with low toxicity.Thus,BA might be a potential novel therapeutic agent for CRC.
文摘The aim of this study was to evaluate the effect of naringin on experimentally induced inflammatory bowel dis- ease in rats. Naringin （20, 40 and 80 mg/kg） was given orally for 7 days to Wistar rats before induction of colitis by intrarectal instillation of 2 mL of 4% （v/v） acetic acid solution. The degree of colonic mucosal damage was analyzed by examining mucosal damage, ulcer area, ulcer index and stool consistency. Intrarectal administration of 4% acetic acid resulted in significant modulation of serum alkaline phosphatase, lactate dehydrogenase, superoxide dismutase （SOD）, glutathione （GSH）, malondialdehyde （MDA） and myeloperoxidase （MPO） content along with colonic nitric oxide （NO）, xanthine oxidase （XO） level and protein carbonyl content in the colonic tissue as well as in blood. Naringin （40 and 80 mg/kg） exerted a dose dependent （P 〈 0.05） ameliorative effect, as it significantly increased hematological parameter as well as colonic SOD and GSH. There was a significant （P 〈 0.05） and dose dependant inhibition of macroscopical score, ulcer area along with colonic MDA, MPO activity by the 7 days of pretreatment of naringin （40 and 80 mg/kg）. Biochemical studies revealed a significant （P 〈 0.05） dose dependant inhibition in serum alkaline phosphatase （ALP） and lactate dehydrogenase （LDH） levels by pretreatment of naringin. Increased levels of colonic NO, XO, protein carbonyl content and DNA damage were also sig- nificantly decreased by naringin pretreatment. The findings of the present investigation propose that naringin has an anti-inflammatory, anti-oxidant and anti-apoptotic potential effect at colorectal sites as it modulates the production and expression of oxidative mediators such as MDA, MPO, NO and XO, thus reducing DNA damage.
文摘Some new complexes RE2(EDODA)3·3H2O, where RE = La, Nd, Eu, Gd, Tb, Er, Yb, Lu and Y, EDODA = ethylene-1,2-dioxydiacetate, have been synthesized and characterized by elemental analysis, molar conductance, IR spectra, UV spectra, TG-DTA, 1H NMR and 13C NMR spectra. Various analyses indicate that the complexes are of nine-coordinated binuclear structure. The carboxylates are bidentate ligands and the ether oxygen atoms also coordinate to rare earth ions. Three water molecules are crystalline water. In addition, the influence of concentration on the chemical shift has been studied through the 1H NMR spectra of the complex Lu2(EDODA)3·3H2O in different concentrations.
