The Crystals of acidic phospholipase A2 from the venom of Agkistrodon blomhoffii bre-vicaudus (i.e. Agkistrodon halys pallas) covalently modified with p-bromo-phenacylbromide wereobtained by the method of hanging drop...The Crystals of acidic phospholipase A2 from the venom of Agkistrodon blomhoffii bre-vicaudus (i.e. Agkistrodon halys pallas) covalently modified with p-bromo-phenacylbromide wereobtained by the method of hanging drop vapor diffusion. Crystallization droplet was composedof 0.06mol’L-1 Na(CH3)2AsO2 (pH=6.5), 24.8% (v/v) 1,4-butanediol, and 4.5mg.mL-1 protein.The crystal data are ot a=b=82.82A., c=32.85A, space group P61. 8945 unique reflections with1.93A resolution were ineasured on a Siemens area detector X-ray diffractometor, of which 8069reflections haring F0 >2σ(F). The experimental results show that the crystals are suitable to astructure analysis of high resolution.展开更多
Co(bpb) [bpbH2is N,N′-o-phenylenebis(pyridine-2-carboxamide) ,C18H12N4O2] complex has active axial sites like a porphyrin complex. We studied the coordination of azide ion(N3-) to Co(bpb) in binary methanol-water mix...Co(bpb) [bpbH2is N,N′-o-phenylenebis(pyridine-2-carboxamide) ,C18H12N4O2] complex has active axial sites like a porphyrin complex. We studied the coordination of azide ion(N3-) to Co(bpb) in binary methanol-water mixtures by spectrophotometric method at the temperature range of 283-303 K. From the temperature dependence of the rate constant,activation parameters(Ea,ΔH,ΔS,and ΔG) were obtained. An isokinetic temperature at about 302 K was observed at which the formation rate of Co(bpb) -N3-was more or less independent of the solvent composition. The resulting ΔH against TΔS plot showed a good linear correlation,indicating the existence of enthalpy-entropy compensation in azide complexation process. Under optimum conditions and based on the absorbance of Co(bpb) -N3-produced through complex formation,a spectrophotometric method for the determination of N 3-in solution was developed. A linear relationship between the absorbance and N3-concentration was obtained in the range of(0.85-5.00) × 10-4 mol·L-1(3.6-21.0 μg·mL-1) . The detection limit was 2.5×10-5 mol·L-1(1.0 μg·mL-1) . The proposed method was applied to the determination of N3-anion in real water samples.展开更多
文摘The Crystals of acidic phospholipase A2 from the venom of Agkistrodon blomhoffii bre-vicaudus (i.e. Agkistrodon halys pallas) covalently modified with p-bromo-phenacylbromide wereobtained by the method of hanging drop vapor diffusion. Crystallization droplet was composedof 0.06mol’L-1 Na(CH3)2AsO2 (pH=6.5), 24.8% (v/v) 1,4-butanediol, and 4.5mg.mL-1 protein.The crystal data are ot a=b=82.82A., c=32.85A, space group P61. 8945 unique reflections with1.93A resolution were ineasured on a Siemens area detector X-ray diffractometor, of which 8069reflections haring F0 >2σ(F). The experimental results show that the crystals are suitable to astructure analysis of high resolution.
基金supported by the Tarbiat Modares University Research Council.
文摘Co(bpb) [bpbH2is N,N′-o-phenylenebis(pyridine-2-carboxamide) ,C18H12N4O2] complex has active axial sites like a porphyrin complex. We studied the coordination of azide ion(N3-) to Co(bpb) in binary methanol-water mixtures by spectrophotometric method at the temperature range of 283-303 K. From the temperature dependence of the rate constant,activation parameters(Ea,ΔH,ΔS,and ΔG) were obtained. An isokinetic temperature at about 302 K was observed at which the formation rate of Co(bpb) -N3-was more or less independent of the solvent composition. The resulting ΔH against TΔS plot showed a good linear correlation,indicating the existence of enthalpy-entropy compensation in azide complexation process. Under optimum conditions and based on the absorbance of Co(bpb) -N3-produced through complex formation,a spectrophotometric method for the determination of N 3-in solution was developed. A linear relationship between the absorbance and N3-concentration was obtained in the range of(0.85-5.00) × 10-4 mol·L-1(3.6-21.0 μg·mL-1) . The detection limit was 2.5×10-5 mol·L-1(1.0 μg·mL-1) . The proposed method was applied to the determination of N3-anion in real water samples.
