Two different species, Trichoderma viride TV10 and Trichoderma harzianum TH12 from 30 Trichoderma isolates were selected out based on their high growth inhibition of the phytopathogen Sclerotinia sclerotiorum (Lib) de...Two different species, Trichoderma viride TV10 and Trichoderma harzianum TH12 from 30 Trichoderma isolates were selected out based on their high growth inhibition of the phytopathogen Sclerotinia sclerotiorum (Lib) de Bary, which reached 84.44% and 100%, respectively. Their untreated culture filtrates (CF) and culture filtrates treated with heat (CFH) also were tested for growth inhibition of the pathogen in potato dextrose agar (PDA). Morphological and molecular characterisation by internal transcribed spacer (ITS) PCR provided consistent identification of these isolates. The degree of infection and disease index (DI) of S. sclerotiorum were examined in Brassica napus (AACC) and Raphanus alboglabra (RR) and Brassica alboglabra (CC). The results revealed that Raphanus alboglabra showed higher disease resistance than that of B. napus. Biotic elecitors T. harzianum TH12 and T. viride TV10 and their CF and CFH demonstrated the ability to cause induced systemic resistance (ISR) in B. napus and Raphanus alboglabra against sclerotinia stem rot (SSR) disease. Furthermore, a high ability to reduce the degree of infection and DI in B. napus with the biotic elicitors T. harzianum TH12 and T. viride TV10 was observed, with numbers reaching 7.22% to 6.67% and 17.78% to 11.67%, respectively. When CF were used, reached 20.00% to 16.67% and 33.33% to 23.33%, respectively;with CFH, values reached 35.00% to 21.67% and 37.78% to 28.33%, respectively. While in Raphanus alboglabra the degree of infection and DI reached 0.00% and 0.00% with all biotic elicitors treatments. These results show that biotic elicitor treatments significantly (P B. napus and Raphanus alboglabra ranked as most effective. This study showed for the first time the ability of genotype Raphanus alboglabra (RRCC) to demonstrate resistance against S. sclerotiorum with or without treatment by biotic elicitors and the ability of genotype B. napus (AACC) to demonstrate resistance to the pathogen after treatment with biotic elicitors.展开更多
选用棉花枯萎菌、黄瓜腐霉菌和白菜根系非致病镰刀菌作为指示菌株,在马铃薯蔗糖琼脂培养基上测定芥蓝(Brassica alboglabra L. H. Bailey)、结球甘蓝(B. oleraceavar. capitata)、白花椰菜(B. oleracea L. var. botry-tisL)、球茎甘蓝(B...选用棉花枯萎菌、黄瓜腐霉菌和白菜根系非致病镰刀菌作为指示菌株,在马铃薯蔗糖琼脂培养基上测定芥蓝(Brassica alboglabra L. H. Bailey)、结球甘蓝(B. oleraceavar. capitata)、白花椰菜(B. oleracea L. var. botry-tisL)、球茎甘蓝(B. oleracea var. gongylodes)、苦菜[B. integrifolia (West) O.E.Schulz]、芹菜(Apium graveolens L.)、黄瓜(Cucumis sativus L.)和大葱(Allium fistulosum L.)的生物熏蒸能力。结果表明,不同材料的组织捣碎粉末对3种菌均具有抑制作用,其中芸苔属植物的熏蒸抑菌效果好于非芸苔属植物,球茎甘蓝熏蒸效果较稳定,且效果最好,在第7 d对棉花枯萎病菌的抑制效果仍然达91.9%;但各植物抑菌效果均随着培养时间的延长而逐渐下降。展开更多
本试验利用6个单,双低甘蓝型油菜品种作为亲本,研究了14个产量、品质性状的超亲优势和12个性状的配合力,同时计算了单株种子产量优势强度和其它11个性状优势强度间的相关系数和通径系数。结果表明:a.甘蓝型油菜F_1杂种的超亲优势普遍存...本试验利用6个单,双低甘蓝型油菜品种作为亲本,研究了14个产量、品质性状的超亲优势和12个性状的配合力,同时计算了单株种子产量优势强度和其它11个性状优势强度间的相关系数和通径系数。结果表明:a.甘蓝型油菜F_1杂种的超亲优势普遍存在,以单株种子产量平均超亲优势值最高,分别为37.1%和34.3%;亲本不同来源(亚洲油菜×欧洲油菜)的组合表现的绝对值和超亲优势在主要农艺性状方面高于来源相同的(亚洲油菜×亚洲油菜或欧洲油菜×欧洲油菜)组合.b.12个性状的g. c. a. 方差和s. c. a方差均十分显着,说明加性效应和非加性效应对这些性状的的遗传变异影响显著.c.甘蓝型油菜单株产量的配合力效应是产量直接构成因素配合力互补的结果。特殊配合力效应与超亲优势关系密切,可以用s. c. a. 效应大致预测杂种优势,而g. c. a. 效应与其后代的超亲优势之间无明显规律可循。d.单株种子产量超亲优势与一次有效分枝数、二次有效分枝数和单株有效角果数超亲优势呈极显著正相关,与其它性状优势相关不显著。展开更多
文摘Two different species, Trichoderma viride TV10 and Trichoderma harzianum TH12 from 30 Trichoderma isolates were selected out based on their high growth inhibition of the phytopathogen Sclerotinia sclerotiorum (Lib) de Bary, which reached 84.44% and 100%, respectively. Their untreated culture filtrates (CF) and culture filtrates treated with heat (CFH) also were tested for growth inhibition of the pathogen in potato dextrose agar (PDA). Morphological and molecular characterisation by internal transcribed spacer (ITS) PCR provided consistent identification of these isolates. The degree of infection and disease index (DI) of S. sclerotiorum were examined in Brassica napus (AACC) and Raphanus alboglabra (RR) and Brassica alboglabra (CC). The results revealed that Raphanus alboglabra showed higher disease resistance than that of B. napus. Biotic elecitors T. harzianum TH12 and T. viride TV10 and their CF and CFH demonstrated the ability to cause induced systemic resistance (ISR) in B. napus and Raphanus alboglabra against sclerotinia stem rot (SSR) disease. Furthermore, a high ability to reduce the degree of infection and DI in B. napus with the biotic elicitors T. harzianum TH12 and T. viride TV10 was observed, with numbers reaching 7.22% to 6.67% and 17.78% to 11.67%, respectively. When CF were used, reached 20.00% to 16.67% and 33.33% to 23.33%, respectively;with CFH, values reached 35.00% to 21.67% and 37.78% to 28.33%, respectively. While in Raphanus alboglabra the degree of infection and DI reached 0.00% and 0.00% with all biotic elicitors treatments. These results show that biotic elicitor treatments significantly (P B. napus and Raphanus alboglabra ranked as most effective. This study showed for the first time the ability of genotype Raphanus alboglabra (RRCC) to demonstrate resistance against S. sclerotiorum with or without treatment by biotic elicitors and the ability of genotype B. napus (AACC) to demonstrate resistance to the pathogen after treatment with biotic elicitors.
文摘选用棉花枯萎菌、黄瓜腐霉菌和白菜根系非致病镰刀菌作为指示菌株,在马铃薯蔗糖琼脂培养基上测定芥蓝(Brassica alboglabra L. H. Bailey)、结球甘蓝(B. oleraceavar. capitata)、白花椰菜(B. oleracea L. var. botry-tisL)、球茎甘蓝(B. oleracea var. gongylodes)、苦菜[B. integrifolia (West) O.E.Schulz]、芹菜(Apium graveolens L.)、黄瓜(Cucumis sativus L.)和大葱(Allium fistulosum L.)的生物熏蒸能力。结果表明,不同材料的组织捣碎粉末对3种菌均具有抑制作用,其中芸苔属植物的熏蒸抑菌效果好于非芸苔属植物,球茎甘蓝熏蒸效果较稳定,且效果最好,在第7 d对棉花枯萎病菌的抑制效果仍然达91.9%;但各植物抑菌效果均随着培养时间的延长而逐渐下降。
文摘本试验利用6个单,双低甘蓝型油菜品种作为亲本,研究了14个产量、品质性状的超亲优势和12个性状的配合力,同时计算了单株种子产量优势强度和其它11个性状优势强度间的相关系数和通径系数。结果表明:a.甘蓝型油菜F_1杂种的超亲优势普遍存在,以单株种子产量平均超亲优势值最高,分别为37.1%和34.3%;亲本不同来源(亚洲油菜×欧洲油菜)的组合表现的绝对值和超亲优势在主要农艺性状方面高于来源相同的(亚洲油菜×亚洲油菜或欧洲油菜×欧洲油菜)组合.b.12个性状的g. c. a. 方差和s. c. a方差均十分显着,说明加性效应和非加性效应对这些性状的的遗传变异影响显著.c.甘蓝型油菜单株产量的配合力效应是产量直接构成因素配合力互补的结果。特殊配合力效应与超亲优势关系密切,可以用s. c. a. 效应大致预测杂种优势,而g. c. a. 效应与其后代的超亲优势之间无明显规律可循。d.单株种子产量超亲优势与一次有效分枝数、二次有效分枝数和单株有效角果数超亲优势呈极显著正相关,与其它性状优势相关不显著。
