Comparison between Four Types of Buffers for DNA Agarose Gel Electrophoresis

To detect the genome of viruses (in environmental and clinical samples), we use electrophoresis running buffer after PCR reaction. Also, electrophoresis buffers were used widely to separate any DNA molecule. In this paper, we used four types of previously known electrophoresis buffers to compare which is easy for preparation, simple in structure, low cost and good performance in agarose gel electrophoresis. For this, we used two agarose concentration (1%, 2%) and two types of DNA ladder (100 bp, 1 kb) represent both smaller and larger sizes of molecule for each type of buffers, from the result we found in first level both supper buffer and TAE buffer with good performance and in second level we found bicarbonate buffer also with good performance also. Finally, we found the tang buffer cannot pose any electrophoretic activity on DNA agarose gel electrophoresis.

BUFFERS的优化设置

设置BUFFERS数目并减少常规内存占用的技巧。

Protocol Buffers——比XML快近100倍

文章介绍了Google公司开发的一种新的数据描述语言—Protocol Buffers,说明了Protocol Buffers的定义、来源、发展和特点,且对Protocol Buffers和XML进行了比较,阐述了Protocol Buffers是如何工作的。

基于Protocol Buffers的配置下发接口适配与应用

简述了网管接口技术的发展现状,对现有网管接口技术使用的编码方式做出了比较;介绍了一种简单快速的序列化机制Protocol Buffers的基本功能与特点;提出了一种采用Protocol Buffers作为编码方式的网管配置下发模型,设计了一个Protocol Buffers与现有命令行(CLI)接口进行适配的适配层,给出了其工作原理与部署方法。

Protocol Buffers在数据采集与传输系统中的应用

随着传感器类型的多样化,采集和传输的数据类型也越来越多样化。这也要求数据采集与传输系统能够方便快捷的对不同类型的传感器和传输数据类型进行扩展。在基于Protocol Buffers的数据采集与传输系统中,通过使用Protocol Buffers作为数据序列化和反序列化的手段,并利用其反射机制等实现系统采集数据类型的快速扩展。

An effective discrete artificial bee colony algorithm for flow shop scheduling problem with intermediate buffers

An effective discrete artificial bee colony(DABC) algorithm is proposed for the flow shop scheduling problem with intermediate buffers(IBFSP) in order to minimize the maximum completion time(i.e makespan). The effective combination of the insertion and swap operator is applied to producing neighborhood individual at the employed bee phase. The tournament selection is adopted to avoid falling into local optima, while, the optimized insert operator embeds in onlooker bee phase for further searching the neighborhood solution to enhance the local search ability of algorithm. The tournament selection with size 2 is again applied and a better selected solution will be performed destruction and construction of iterated greedy(IG) algorithm, and then the result replaces the worse one. Simulation results show that our algorithm has a better performance compared with the HDDE and CHS which were proposed recently. It provides the better known solutions for the makespan criterion to flow shop scheduling problem with limited buffers for the Car benchmark by Carlier and Rec benchmark by Reeves. The convergence curves show that the algorithm not only has faster convergence speed but also has better convergence value.

Timing Optimization by Inserting Minimum Buffers with Accurate Delay Models

An algorithm of minimizing the number of buffers under certain delay constraint with accurate delay models is presented.Given a two-terminal net,the algorithm can minimize the total number of buffers inserted to meet the delay constraint.A high order delay model is applied to estimate interconnect delay and a nonlinear delay model based on look-up table is for buffer delay estimation.The experimental results show that the algorithm can efficiently achieve the trade-offs between number of buffers and delay,and avoid needless power and area cost.The running time is satisfactory.

SYSTEM AGGREGATION METHOD FOR FAILURE PRONE PRODUCTION LINES WITH UNRELIABLE LIMITED BUFFERS

Different from traditional aggregation method, the unreliable buffers are originally considered and a more general aggregation method is offered, in which not only the unreliable buffers are considered, but also the probabilities of system states are obtained by a discrete model rather than the continuous flow model of unreliable manufacturing systems. The solution technique is offered to get the system sate probabilities. The method advances the traditional system aggregation techniques. Numerical results specify the extended aggregation method and also show that the unreliable limited buffers have a strong impact on the efficiency of the production lines.

Hybrid Discrete Harmony Search Algorithm for Flow Shop Scheduling with Limited Buffers

The flow shop scheduling problem with limited buffers( LBFSP) widely exists in manufacturing systems. A hybrid discrete harmony search algorithm is proposed for the problem to minimize total flow time. The algorithm presents a novel discrete improvisation and a differential evolution scheme with the jobpermutation-based representation. Moreover,the discrete harmony search is hybridized with the problem-dependent local search based on insert neighborhood to balance the global exploration and local exploitation. In addition, an orthogonal experiment design is employed to provide a receipt for turning the adjustable parameters of the algorithm. Comparisons based on the Taillard benchmarks indicate the superiority of the proposed algorithm in terms of effectiveness and efficiency.

Limited Delay Preemption Based Priority in Differentiated Optical Burst Switching Networks with Small Buffers

The composite scheme based on preemption and small buffers is an efficient method for contention resolution. To support services differentiation, it is the first time that the analytical model of delay preemption based priority is built. Further, in order to guarantee the low-loss requirement for high priority bursts, an improved scheme is proposed and investigated by limiting the buffered right of low priority bursts within the specific traffic states. The simulation results show that, without the deterioration of blocking performance, there is more than 40% reduction on burst loss being achieved under the conditionρ=1.0 for high priority bursts.

A Novel BIST Approach for Testing Input/Output Buffers in SoCs

A novel built-in self-test （BIST） approach to test the configurable input/output buffers in Xilinx Virtex series SoCs （system on a chip） using hard macro has been proposed in this paper. The proposed approach can completely detect single and multiple stuck-at gate-level faults as well as associated routing resources in I/O buffers. The proposed BIST architecture has been implemented and verified on Xilinx Virtex series FPGAs （field programmable gate configurations are required array）. Only total of 10 to completely test the I/O buffers of Virtex devices.

Increase of brightness and transmission efficiency in fiat panel display through serial synchronous scanning mode with double buffers

This paper presents a serial synchronous scanning mode in fiat panel display （FPD） by adding a latch buffer between the serializer and the driving buffer. Comparing with conventional techniques, the proposed structure can efficiently reduce the brightness loss and improve the transmission performance. Theoretical analysis and experimental results show that the ratio between the lightest weight display time and the relative transmission time is a tradeoff between brightness loss and transmission efficiency.

0.6-eV bandgap In_(0.69)Ga_(0.31)As thermophotovoltaic devices with compositionally undulating step-graded InAsyP_(1-y)buffers

Single-junction,lattice-mismatched In0.69Ga0.31As thermophotovoltaic（TPV） devices each with a bandgap of 0.6 eV are grown on InP substrate by metal-organic chemical vapour deposition（MOCVD）.Compositionally undulating stepgraded InAsyP1-y buffer layers with a lattice mismatch of ～1.2% are used to mitigate the effect of lattice mismatch between the device layers and the InP substrate.With an optimized buffer thickness,the In0.69Ga0.31As active layers grown on the buffer display a high crystal quality with no measurable tetragonal distortion.High-performance single-junction devices are demonstrated,with an open-circuit voltage of 0.215 V and a photovoltaic conversion efficiency of 6.9% at a short-circuit current density of 47.6 mA/cm2,which are measured under the standard solar simulator of air mass 1.5-global（AM 1.5 G）.

Landscape fragmentation associated with the Qingzang Highway and its influencing factors-A comparison study on road sections and buffers

Many studies have examined the effect of roads on landscape fragmentation.Yet they rarely considered local characteristics of the road and road buffer widths.Therefore,this study that took place in the Qingzang Highway(QH)examined the variations in road buffers and road sections of landscape fragmentation.The QH was divided into 32 sections with 23 buffer areas.Based on the indicators of landscape fragmentation from 1980 to 2018,we found significant spatial heterogeneity between sections and buffers.Generally,landscape fragmentation de-creased with increasing buffer distance to the QH.For different sections,the coefficients of variation between buffers were rather high and significantly different.Therefore,fixed-width buffers may overestimate or underes-timate the spatial scope and influence intensity of a road.The impacts of road sections around provincial capitals,prefecture-level cities and main counties on landscape fragmentation were relatively extensive and formed clus-ters of highly fragmented areas.Geodetector results indicated that natural and anthropogenic factors,such as altitude,climate,distance to major settlements and socioeconomic conditions,could well explain the spatiotem-poral characteristics of landscape fragmentation.Altitude,precipitation and the distance to major settlements had higher explanatory power for landscape fragmentation in permafrost regions,whereas slope and socioeconomic condition had higher explanatory power for non-permafrost regions in Xizang Autonomous Region.

Growth and Characterization of Doped CeO_2 Buffers on Ni-W Substrates for Coated Conductors Using Metal Organic Deposition Method

CeO2 and Ce0.8M0.2O2-d films （M = Mn, Y, Gd, Sm, Nd and La） with （00l） preferred orientation have been prepared on biaxially textured Ni-W substrates by metal organic decomposition （MOD） method. The factors influencing the formation of cracks on the surface of these CeO2 and doped CeO2 films on Ni-W substrates were explored by X-ray diffraction （XRD）, scanning electron microscopy （SEM） analysis, atomic force microscopy （AFM） and differential scanning calorimetry （DSC）. The results indicate that many factors, such as the change of the ionic radii of doping cations, the transformation of crystal structure and the formation of oxygen vacancies in lattices at high annealing temperature, may be related to the formation of cracks on the surface of these films. However, the crack formation shows no dependence on the crystal lattice mismatch degree of the films with Ni-W substrates. Moreover, the suppression of surface cracks is related to the change of intrinsic elasticity of CeO2 film with doping of cations with a larger radius. SEM and AFM investigations of Ce0.8M0.2O2-d （M = Y, Gd, Sm, Nd and La） films reveal the dense, smooth and crack-free microstructure, and their lattice parameters match well with that of YBCO, illuminating that they are potentially suitable to be as buffer layer, especially as cap layer in multi-layer architecture of buffer layer for coated conductors.

PARASITIC TOLERANT DIFFERENTIAL SC TRANSCONDUCTANCE USING UNITY GAIN BUFFERS

The unity gain buffer will be good to design high frequency SCF if its resistiveeffects can be eliminated,and therefore the whole parasitic sensitivities will greatly be reduced.On the basis of this concept,a novel parasitic tolerant SC DTE(differential transconductanceelement)is proposed.SC floating inductor and integrator fit for high frequency applications areformed by the DTE.The computer simulation and experiment on a third order elliptic LP filterverify its validity.

Investigation on performance of all optical buffer with large dynamical delay time based on cascaded double loop optical buffers

Optical buffers are critical for optical signal processing in future optical packet-switched networks. In this paper, a theoretical study as well as an experimental demonstration on a new optical buffer with large dynamical delay time is carried out based on cascaded double loop optical buffers （I：）LOBs）. It is found that pulse distortion can be restrained by a negative optical control mode when the optical packet is in the loop. Noise analysis indicates that it is feasible to realise a large variable delay range by cascaded DLOBs. These conclusions are validated by the experiment system with 4-stage cascaded DLOBs. Both the theoretical simulations and the experimental results indicate that a large delay range of 1-9999 times the basic delay unit and a fine granularity of 25 ns can be achieved by the cascaded DLOBs. The performance of the cascaded DLOBs is suitable for the all optical networks.

Comparative Study on Riparian Vegetation Buffers in Different Land Uses

This study focused on riparian vegetation buffer zones on the basis of low influence development(LID) and landscape restoration. Three types of land were selected to conduct comparative study. The types are buffers in urban districts,buffers in rural areas,and buffers in natural regions. These types basically reflect major ecological issues in different landuses. The follow conclusions were drawn:(1) there are different ecological issues in these 3 types,making the functions of buffers different.(2) The 3 types demand different ecological issues to be solved: Type A,functionally-diverse riparian landscape should be planned to enrich recreational activities and improve river's condition. Type B,agricultural areasource pollution needs to be cut down to sustain the stability of water conservation and ecological system. Type C,eco-conservation and various habitats for creatures are required.(3) Different planning methods were used respectively in the 3 types,construction methods were proposed for each type correspondingly and expressed in graphical ways in expectation of being instructive to the construction of eco-riparian landscape.

Creek Proximity and Flora Effects on Arthropod Populations in Riparian Buffers in Washington State, USA

Due to federal, state, and local regulations pertaining to clean water and salmon recovery, stream health and water quality are major concerns in the Pacific Northwest of the United States. An integral component of most riparian rehabilitation efforts is the effective establishment of preferred vegetation. We conducted arthropod surveys in representative disturbed and rehabilitated riparian buffers directly adjacent to apple orchards and grape vineyards in the Lower Yakima Valley near Prosser, Benton County, Washington State USA. Objectives of the study were to determine whether densities of select predatory and pest taxa changed with distance from the waterway and to test whether densities differed among disturbed, rehabilitated, or pristine riparian buffers. The studies suggest that populations of some beneficial arthropods, including spiders and ground beetles, were higher in the presence of exotic flowering plant species. In the riparian environments surveyed, more native woody plant species were found adjacent to the waterway, and this apparent stability appears to have had a positive effect on beneficial arthropods. Concurrently, data indicated that some exotic, flowering species of plants may be preferred over native plant species as hosts to certain arthropod pests. These plants tend to persist in degraded riparian buffers and at the interface of riparian habitats and cultivated areas, whereas native plant species are predominant in pristine or properly maintained and rehabilitated buffers.