The result of the study contributes to enhancing and sustaining future livelihoods and food security in Vietnam vis-a-vis climate change. An innovative strategy based on marker-assisted backcrossing (MABC) was used to...The result of the study contributes to enhancing and sustaining future livelihoods and food security in Vietnam vis-a-vis climate change. An innovative strategy based on marker-assisted backcrossing (MABC) was used to transform popular rice variety AS996 into the one can tolerate submergence while maintaining its original characteristics preferred by farmers and consumers. The submergence tolerance QTL SUB1 counts for up to 70% of the submergence tolerant and provides a marked improvement of submergence tolerance in all genetic backgrounds and environments tested so far. Parental diversity was carried out with 460 markers. Of which, 53 polymorphic markers were used for assessment on BC1F1, BC2F1 and BC3F1 generations. The best BC1F1 plant was P422 with highest recipient allele was 87.5%, while the additional chosen plants were P412, P428, P215 and P39 (81% - 84%). All these plants were used to develop BC2F1 generation. The six BC2F1 plants were used to develop BC3F1 and BC2F2 were the plants number P422-11 and P422-14 having 93.75% recipient alleles and P422-12, P422-3, P39-17, P39-25 having 92.25% recipient alleles. Total of 445 BC3F1 plants were confirmed the introgresion of SUB1 using ART5 and SC3. After three generations of backcrossing, application of MABC resulted in the best BC3F1 individual P422-14-177 with 100% of recipient alleles based on the number of 53 markers used with only the introgression size of SUB1 was 0.3Mb between ART5 and SC3. Phenotyping was carried out on BC3F1 and BC2F2 of the selected lines. The survival ratio of these selected lines and IR64SUB1 were the same. It convinced the successfully introgress SUB1 into AS996 rice variety. The breeding line BC4F1 having 100% genetic background of donor variety is ready for develop new submergence tolerant rice variety ASS996-SUB1 to cope with climate change.展开更多
The breeding activities aimed to create a natural green cocoon variety for spring rearing with the characteristics of stable natural cocoon color,easy rearing and good silk quality.Four different lines from the excell...The breeding activities aimed to create a natural green cocoon variety for spring rearing with the characteristics of stable natural cocoon color,easy rearing and good silk quality.Four different lines from the excellent silkworm variety Jingsong×Haoyue were taken as recurrent parents,using the natural green cocoon variety G 9202 as donor parent.After other excellent parents were introduced to cross with these different lines,four new breeding materials were selected by recurrent backcross breeding.The characters of these breeding materials showed not only natural green cocoon but also excellent comprehensive traits.A naturally colored cocoon quaternary hybrid with excellent practical characters was selected based on multiple hybrids.It is suitable for spring rearing in Yangtze valley which includes Hunan Province.It was approved by Hunan Provincial Crop Variety Approval Committee and designated Xiangcailu No.1 in 2016.展开更多
The cytochrome bc1complex(the bc1complex or complex Ⅲ) is an attractive target for the discovery of numerous pharmaceuticals and pesticides. In order to identify new lead structures for this target, a new series of m...The cytochrome bc1complex(the bc1complex or complex Ⅲ) is an attractive target for the discovery of numerous pharmaceuticals and pesticides. In order to identify new lead structures for this target, a new series of molecules, N-(4-aryloxyphenyl)phthalimides, were designed and synthesized in a straightforward manner. Our design strategy was to introduce a 4-aryloxyphenyl group, a fragment which exhibited promising bc1complex-inhibiting properties, into the aryl group of the valuable N-arylphthalimide backbone. Afterward, the biochemical evaluation of the newly synthesized compounds was carried out,and the results implied that several compounds demonstrated good activities against succinatecytochrome reductase(SCR, a mixture of mitochondrial complex Ⅱ and the bc1complex). Further studies confirmed that 3e’, a representative compound in this paper, was identified as an inhibitor of the bc1complex. Furthermore, computational simulations were also performed to better understand binding of 3e’ to the enzyme complex, which indicated that 3e’ should bind to the Qosite of the bc1complex.Consequently, we harbor the idea that this paper can provide a solid platform for synthesis and discovery of other bc1complex inhibitors.展开更多
In searching for novel fungicidal leads,a series of pyrazole-containing strobilurins were rationally designed,synthesized and charac-terized.Bioassay indicated that compound 1-7 displayed excellent fungicidal activity...In searching for novel fungicidal leads,a series of pyrazole-containing strobilurins were rationally designed,synthesized and charac-terized.Bioassay indicated that compound 1-7 displayed excellent fungicidal activity against a broad spectrum of plant pathogens such as Gibberella zeae,Rhizoctonia cereolis,Sclerotinia sclerotiorum,Phytophthora infestans,Physolosporo piricola and Pellicularia sosakii with EC_(50) of 0.16,0.02,0.72,0.07,0.77 and 0.65μg/mL,respectively,which were 3-10 times more potent than the positive control azoxystrobin against the corresponding pathogens.Moreover,like azoxystrobin and kresoxim methyl,1-7,displayed excellent protec-tive activity against P.sorghi.Molecular docking validated that 1-7 and azoxystrobin would share a similar binding mode with cytochrome bc_(1) complex.This study demonstrates that 1-7 is a promising fungicidal candidate for further development.展开更多
文摘The result of the study contributes to enhancing and sustaining future livelihoods and food security in Vietnam vis-a-vis climate change. An innovative strategy based on marker-assisted backcrossing (MABC) was used to transform popular rice variety AS996 into the one can tolerate submergence while maintaining its original characteristics preferred by farmers and consumers. The submergence tolerance QTL SUB1 counts for up to 70% of the submergence tolerant and provides a marked improvement of submergence tolerance in all genetic backgrounds and environments tested so far. Parental diversity was carried out with 460 markers. Of which, 53 polymorphic markers were used for assessment on BC1F1, BC2F1 and BC3F1 generations. The best BC1F1 plant was P422 with highest recipient allele was 87.5%, while the additional chosen plants were P412, P428, P215 and P39 (81% - 84%). All these plants were used to develop BC2F1 generation. The six BC2F1 plants were used to develop BC3F1 and BC2F2 were the plants number P422-11 and P422-14 having 93.75% recipient alleles and P422-12, P422-3, P39-17, P39-25 having 92.25% recipient alleles. Total of 445 BC3F1 plants were confirmed the introgresion of SUB1 using ART5 and SC3. After three generations of backcrossing, application of MABC resulted in the best BC3F1 individual P422-14-177 with 100% of recipient alleles based on the number of 53 markers used with only the introgression size of SUB1 was 0.3Mb between ART5 and SC3. Phenotyping was carried out on BC3F1 and BC2F2 of the selected lines. The survival ratio of these selected lines and IR64SUB1 were the same. It convinced the successfully introgress SUB1 into AS996 rice variety. The breeding line BC4F1 having 100% genetic background of donor variety is ready for develop new submergence tolerant rice variety ASS996-SUB1 to cope with climate change.
基金Supported by Earmarked Fund for China Agriculture Research System(CARS-18)Hunan Provincial Science and Technology Support Program(2013NK3071)+1 种基金Natural Science Foundation of Hunan Province(2017JJ2137)Silkworm Germplasm Resources Multi-element Application Research and Development Innovation Team(2017XC01)
文摘The breeding activities aimed to create a natural green cocoon variety for spring rearing with the characteristics of stable natural cocoon color,easy rearing and good silk quality.Four different lines from the excellent silkworm variety Jingsong×Haoyue were taken as recurrent parents,using the natural green cocoon variety G 9202 as donor parent.After other excellent parents were introduced to cross with these different lines,four new breeding materials were selected by recurrent backcross breeding.The characters of these breeding materials showed not only natural green cocoon but also excellent comprehensive traits.A naturally colored cocoon quaternary hybrid with excellent practical characters was selected based on multiple hybrids.It is suitable for spring rearing in Yangtze valley which includes Hunan Province.It was approved by Hunan Provincial Crop Variety Approval Committee and designated Xiangcailu No.1 in 2016.
基金supported by the National Natural Science Foundation of China(Nos.21502062,21272091 and 21472063)Hubei Provincial Department of Education(No.Q20102606)+3 种基金Xiangyang Science and Technology Bureau(No.2010GG1B33)Structural Biomedicine and Pharmacochemistry of Hubei University of Arts and Sciencethe support from the Russian Foundation for Basic Research(No.18-29-04047)the Tomsk Polytechnic University Competitiveness Enhancement Program grant(No.VIU-195/2018)
文摘The cytochrome bc1complex(the bc1complex or complex Ⅲ) is an attractive target for the discovery of numerous pharmaceuticals and pesticides. In order to identify new lead structures for this target, a new series of molecules, N-(4-aryloxyphenyl)phthalimides, were designed and synthesized in a straightforward manner. Our design strategy was to introduce a 4-aryloxyphenyl group, a fragment which exhibited promising bc1complex-inhibiting properties, into the aryl group of the valuable N-arylphthalimide backbone. Afterward, the biochemical evaluation of the newly synthesized compounds was carried out,and the results implied that several compounds demonstrated good activities against succinatecytochrome reductase(SCR, a mixture of mitochondrial complex Ⅱ and the bc1complex). Further studies confirmed that 3e’, a representative compound in this paper, was identified as an inhibitor of the bc1complex. Furthermore, computational simulations were also performed to better understand binding of 3e’ to the enzyme complex, which indicated that 3e’ should bind to the Qosite of the bc1complex.Consequently, we harbor the idea that this paper can provide a solid platform for synthesis and discovery of other bc1complex inhibitors.
基金the National Natural Science Foundation of China(No.31872007)the Tianjin Natural Science Foundation(No.18JCZDJC33500)+1 种基金the International Science&Technology Cooperation Program of China(No.2014DFR41030)the Tianjin Development Program for Innovation and Entrepreneurship.
文摘In searching for novel fungicidal leads,a series of pyrazole-containing strobilurins were rationally designed,synthesized and charac-terized.Bioassay indicated that compound 1-7 displayed excellent fungicidal activity against a broad spectrum of plant pathogens such as Gibberella zeae,Rhizoctonia cereolis,Sclerotinia sclerotiorum,Phytophthora infestans,Physolosporo piricola and Pellicularia sosakii with EC_(50) of 0.16,0.02,0.72,0.07,0.77 and 0.65μg/mL,respectively,which were 3-10 times more potent than the positive control azoxystrobin against the corresponding pathogens.Moreover,like azoxystrobin and kresoxim methyl,1-7,displayed excellent protec-tive activity against P.sorghi.Molecular docking validated that 1-7 and azoxystrobin would share a similar binding mode with cytochrome bc_(1) complex.This study demonstrates that 1-7 is a promising fungicidal candidate for further development.
