In the high mountain oligotrophic Seven Rila Lakes (2 095–2 535 m a.s.l.) bacterial abundance was recorded in July and September 2006. In July, bacterial abundance (average (3.32±2.5)×105 cell/ml) in the la...In the high mountain oligotrophic Seven Rila Lakes (2 095–2 535 m a.s.l.) bacterial abundance was recorded in July and September 2006. In July, bacterial abundance (average (3.32±2.5)×105 cell/ml) in the lakes correlated negatively with elevation, while in September (average (26.39±7.1)×105 cell/ml) this pattern was altered and the greatest abundance of bacteria occurred at the highest ((39.05±1.5)×105 cell/ml) and the lowest ((30.63±6.0)×105 cell/ml) elevated lakes. Regression analyses suggested that the principal factor controlling bacterial abundance, in both July and September, was inorganic nitrogen (NO3-N and NH4-N; R2=0.70, P<0.05). Other major regulating factors were temperature (R2=0.66, P<0.03) in July and filter-feeding zooplankton (R2=0.95, P<0.01) in September. All factors, except NO3-N, had a positive effect on bacterial abundance.展开更多
The effects of nitrogen （N） addition on microbial biomass, bacterial abundance, and community composition in sediment colonized by Suaeda heteroptera were examined by chloroform fumigation extraction method, real-ti...The effects of nitrogen （N） addition on microbial biomass, bacterial abundance, and community composition in sediment colonized by Suaeda heteroptera were examined by chloroform fumigation extraction method, real-time quantitative polymerase chain reaction, and denaturing gradient gel electrophoresis （DGGE） in a salt marsh located in Shuangtai Estuary, China. The sediment samples were collected from plots treated with different amounts of a single N fertilizer （urea supplied at 0.1, 0.2, 0.4 and 0.8 g/kg （nitrogen content in sediment） and different forms of N fertilizers （urea, （NH4）2SO4, and NH4NO3, each supplied at 0.2 g/kg （calculated by nitrogen）. The fertilizers were applied 1-4 times during the plant-growing season in May, luly, August, and September of 2013. Untreated plots were included as a control. The results showed that both the amount and form of N positively influenced microbial biomass carbon, microbial biomass nitrogen, and bacterial abundance. The DGGE profiles revealed that the bacterial community composition was also affected by the amount and form of N. Thus, our findings indicate that short-term N amendment increases microbial biomass and bacterial abundance, and alters the structure of bacterial community.展开更多
Lead(Pb) and its compounds are common pollutants in industrial wastewaters.To develop appropriate Pb 2+ treatment technologies,aerobic granules(AG) and bacterial alginates(BA) were studied as alternative biosorbents t...Lead(Pb) and its compounds are common pollutants in industrial wastewaters.To develop appropriate Pb 2+ treatment technologies,aerobic granules(AG) and bacterial alginates(BA) were studied as alternative biosorbents to remove Pb 2+ from aqueous solutions.The biosorption mechanism of AG and BA were further analyzed to determine which functional groups in AG and BA are active in Pb 2+ biosorption.In this paper,the Pb 2+ biosorption behavior of AG and BA was respectively investigated in batch experiments from the perspectives of the initial pH,contact time,and initial Pb 2+ concentration.The results showed that biosorption of Pb 2+ by AG and BA occurred within 60min at the initial Pb 2+ concentrations(0 150 mg L-1).The actual saturated Pb 2+ biosorption capability of AG was 101.97 mg g-1(dry weight of aerobic granular biomass).When the initial pH was 5,the biosorption capability of AG and BA was highest at the initial Pb 2+ concentrations(0 20mg L-1).During the process of Pb 2+ biosorption,K +,Ca 2+,and Mg 2+ were released.The Ion Chromatography(IC) and Fourier Transform Infrared Spectroscopy(FTIR) further highlighted the main role of ion exchange between Ca 2+ and Pb 2+ and sequestration of Pb 2+ with carboxyl(-COO) of AG and BA.This analogical analysis verifies that BA is responsible for biosorption of Pb 2+ by AG.At the same optimal pH,AG cultivated with different carbon source has different Pb 2+ biosorption capacity.The Pb 2+ biosorption by AG with sodium acetate as the sole carbon source is higher than AG with glucose as carbon source.展开更多
Based on high resolution,high signal-to-noise(S/N)ratio spectra from Keck/HIRES,we have determined abundances of 20 elements for 18 Ba candidates.The parameter space of these stars is in the range of 4880≤Teff≤6050 ...Based on high resolution,high signal-to-noise(S/N)ratio spectra from Keck/HIRES,we have determined abundances of 20 elements for 18 Ba candidates.The parameter space of these stars is in the range of 4880≤Teff≤6050 K,2.56≤log g≤4.53 dex and-0.27≤[Fe/H]≤0.09 dex.It is found that four of them can be identified as Ba stars with[s/Fe]>0.25 dex(s:Sr,Y,Zr,Ba,La,Ce and Nd),and three of them are newly discovered,which include two Ba giants(HD 16178 and HD 22233)and one Ba subgiant(HD 2946).Our results show that the abundances ofα,odd and iron-peak elements(O,Na,Mg,Al,Si,Ca,Sc,Ti,Mn,Ni and Cu)for our program stars are similar to those of the thin disk,while the distribution of[hs/ls](hs:Ba,La,Ce and Nd,ls:Sr,Y and Zr)ratios of our Ba stars is similar to those of the known Ba objects.None of the four Ba stars show clear enhancement in carbon including the known CH subgiant HD 4395.It is found that three of the Ba stars present clear evidence of hosting stellar or sub-stellar companions from the radial velocity data.展开更多
Rainwater contains substantial bacteria and rain is an efficient pathway for the dissemination of bacteria from the atmosphere to land and water surfaces.However,quantitative information on rainwater bacteria is very ...Rainwater contains substantial bacteria and rain is an efficient pathway for the dissemination of bacteria from the atmosphere to land and water surfaces.However,quantitative information on rainwater bacteria is very limited due to the lack of a reliable method.In this study,the epifluorescence microscopy enumeration with the LIVE/DEAD BacLight Bacterial Viability Kit stain was verified to quantify the abundance of viable and non-viable bacterial cells in rainwater,with the 4＇,6-diamidino-2-phenylindole（DAPI） stain for the reference of total cell counts.Results showed that the total counts of bacterial cells by LIVE/DEAD BacLight staining were consistent with those by DAPI staining,and the average detection efficiency was（109 ± 29）%.The ratio of cell count with glutaraldehyde fixation to that without fixation was（106 ± 5）%on average.The bacterial concentration in negative control was usually an order of magnitude lower than that in rainwater samples.However,in case of small precipitation,the abundance in negative control could be more than that in rainwater samples.These results indicate that the enumeration with LIVE/DEAD BacLight bacterial viability assay coupled with glutaraldehyde fixation and careful negative control investigation is an approach applicable to the measurement of the concentration and viability of bacterial cells in rainwater.展开更多
Low availability of phosphorus(P) is a major constraint for optimal crop production, as P is mostly present in its insoluble form in soil. Therefore,phosphate-solubilizing bacteria(PSB) from paddy field soils of the I...Low availability of phosphorus(P) is a major constraint for optimal crop production, as P is mostly present in its insoluble form in soil. Therefore,phosphate-solubilizing bacteria(PSB) from paddy field soils of the Indo-Gangetic Plain, India were isolated, and their abundance was attempted to be correlated with the physicochemical characteristics of the soils. Ninety-four PSB were isolated on Pikovskaya’s agar medium, and quantitative phosphate solubilization was evaluated using NBRIP medium. The isolates solubilized P up to a concentration of 1 006 μg mL-1 from tricalcium phosphate with the secretion of organic acids. These isolates were identified by 16 S rRNA gene sequence comparison, and they belonged to Gammaproteobacteria(56 isolates),Firmicutes(28 isolates), Actinobacteria(8 isolates), and Alphaproteobacteria(2 isolates). Phylogenetic analysis confirmed the identification by clustering the isolates in the clade of the respective reference organisms. The correlation analysis between PSB abundance and physicochemical characteristics revealed that the PSB population increased with increasing levels of soil organic carbon, insoluble P, K+, and Mg2+. The promising PSB explored in this study can be further evaluated for their biofertilizer potential in the field and for their use as potent bio-inoculants.展开更多
Fildes Peninsula, in King George Island, Antarctica, has a great concentration of international facilities, and it has clearly been affected by human activities. The objective of this 5-year study was to assess the im...Fildes Peninsula, in King George Island, Antarctica, has a great concentration of international facilities, and it has clearly been affected by human activities. The objective of this 5-year study was to assess the impact of anthropogenic activities on the bacterial abundance in water bodies close to Artigas Antarctic Scientific Base （BCAA, in Spanish Base Cientifica Antdrtica Artigas）. Water samples from areas under different human influence （Uruguay Lake, nearby ponds, and meltwater from Collins Glacier） were aseptically collected and refrigerated until processed. The number of heterotrophic bacteria and Pseudomonas spp. was analyzed using a culture-dependent approach. Physico-chemical properties of the water samples （temperature, pH, and conductivity） were also determined. Results showed that water from the highly affected area, Uruguay Lake, where the pump that provides water to the BCAA is located, did not suffer significant fluctuations in heterotrophie bacterial abundance （10^4- 10^5 CFU.mL^-1）; however, Pseudomonas abundance increased until becoming the predominant population. In other water samples, the number of heterotrophie bacteria and Pseudomonas gradually increased during this 5-year study, by 2014 reaching similar values to those observed for Uruguay Lake. The implications of human activities on Antarctic bacterial abundance are discussed.展开更多
Fluoride(F^(-))is widespread in aquatic environments;however,it is not clear whether the fluctuation of F^(-)concentrations in overlying lake water affects the composition and migration of dissolved organic matter(DOM...Fluoride(F^(-))is widespread in aquatic environments;however,it is not clear whether the fluctuation of F^(-)concentrations in overlying lake water affects the composition and migration of dissolved organic matter(DOM)from sediment.A case study was presented in Sand Lake,China,and an experiment was conducted to analyze the influence of different F^(-)concentrations in overlying water on DOM characteristics.Diffusion resulted in similarities in DOM components between overlying and pore waters,and bacterial activities and enzyme variation resulted in differences between them.Higher F^(-)concentrations in overlying water resulted in a higher pH of pore water,which favored the enrichment of protein-like substances.Higher F^(-)concentrations caused lower DOM concentrations and lower maximum fluorescence intensities(Fmax)of protein-like components in pore water.The F^(-)concentrations had significantly negative correlations with Shannon indexes(P<0.05).Thiobacillus influenced the migration of tyrosinelike substances by decreasing the pH of pore water.Trichococcus and Fusibacter altered the Fmax of protein-like,humic-like,and fulvic-like substances.The F^(-)concentrations affected the DOM composition and migration due to the response of functional bacterial communities,which were positively correlated with the relative abundance of Thiobacillus and negatively correlated with the relative abundances of Trichococcus and Fusibacter.The high F^(-)concentrations influenced the biosynthesis and degradation of protein-like substances by shifting the abundances of the relevant enzymes.The results of this study may provide ideas for investigating DOM cycling under the influence of F^(-),especially in lakes with fluctuations in F^(-)concentrations.展开更多
基金Supported by the Sofia University "St. Kl. Ohridski" fund for scientific research (No. 90/2006)
文摘In the high mountain oligotrophic Seven Rila Lakes (2 095–2 535 m a.s.l.) bacterial abundance was recorded in July and September 2006. In July, bacterial abundance (average (3.32±2.5)×105 cell/ml) in the lakes correlated negatively with elevation, while in September (average (26.39±7.1)×105 cell/ml) this pattern was altered and the greatest abundance of bacteria occurred at the highest ((39.05±1.5)×105 cell/ml) and the lowest ((30.63±6.0)×105 cell/ml) elevated lakes. Regression analyses suggested that the principal factor controlling bacterial abundance, in both July and September, was inorganic nitrogen (NO3-N and NH4-N; R2=0.70, P<0.05). Other major regulating factors were temperature (R2=0.66, P<0.03) in July and filter-feeding zooplankton (R2=0.95, P<0.01) in September. All factors, except NO3-N, had a positive effect on bacterial abundance.
基金The National Natural Science Foundation of China under contract No.41171389the Public Science and Technology Research Funds Projects of Ocean under contract No.201305043Program for Liaoning Excellent Talents in University under contract No.LR2013035
文摘The effects of nitrogen （N） addition on microbial biomass, bacterial abundance, and community composition in sediment colonized by Suaeda heteroptera were examined by chloroform fumigation extraction method, real-time quantitative polymerase chain reaction, and denaturing gradient gel electrophoresis （DGGE） in a salt marsh located in Shuangtai Estuary, China. The sediment samples were collected from plots treated with different amounts of a single N fertilizer （urea supplied at 0.1, 0.2, 0.4 and 0.8 g/kg （nitrogen content in sediment） and different forms of N fertilizers （urea, （NH4）2SO4, and NH4NO3, each supplied at 0.2 g/kg （calculated by nitrogen）. The fertilizers were applied 1-4 times during the plant-growing season in May, luly, August, and September of 2013. Untreated plots were included as a control. The results showed that both the amount and form of N positively influenced microbial biomass carbon, microbial biomass nitrogen, and bacterial abundance. The DGGE profiles revealed that the bacterial community composition was also affected by the amount and form of N. Thus, our findings indicate that short-term N amendment increases microbial biomass and bacterial abundance, and alters the structure of bacterial community.
基金supported by the National Natural Science Foundation of China (No. 30570339)the Key Science & Technology Project of the Ministry of Education China (No.106105)
文摘Lead(Pb) and its compounds are common pollutants in industrial wastewaters.To develop appropriate Pb 2+ treatment technologies,aerobic granules(AG) and bacterial alginates(BA) were studied as alternative biosorbents to remove Pb 2+ from aqueous solutions.The biosorption mechanism of AG and BA were further analyzed to determine which functional groups in AG and BA are active in Pb 2+ biosorption.In this paper,the Pb 2+ biosorption behavior of AG and BA was respectively investigated in batch experiments from the perspectives of the initial pH,contact time,and initial Pb 2+ concentration.The results showed that biosorption of Pb 2+ by AG and BA occurred within 60min at the initial Pb 2+ concentrations(0 150 mg L-1).The actual saturated Pb 2+ biosorption capability of AG was 101.97 mg g-1(dry weight of aerobic granular biomass).When the initial pH was 5,the biosorption capability of AG and BA was highest at the initial Pb 2+ concentrations(0 20mg L-1).During the process of Pb 2+ biosorption,K +,Ca 2+,and Mg 2+ were released.The Ion Chromatography(IC) and Fourier Transform Infrared Spectroscopy(FTIR) further highlighted the main role of ion exchange between Ca 2+ and Pb 2+ and sequestration of Pb 2+ with carboxyl(-COO) of AG and BA.This analogical analysis verifies that BA is responsible for biosorption of Pb 2+ by AG.At the same optimal pH,AG cultivated with different carbon source has different Pb 2+ biosorption capacity.The Pb 2+ biosorption by AG with sodium acetate as the sole carbon source is higher than AG with glucose as carbon source.
基金supported by the National Key R&D Program of China (No. 2019YFA0405502)the National Natural Science Foundation of China (Grant Nos. 12090040, 12090044, 11833006, 12022304, 11973052, 11973042, U2031144 and U1931102)+1 种基金supported by the Astronomical Big Data Joint Research Center, co-founded by the National Astronomical Observatories, Chinese Academy of Sciences (CAS)support from the Youth Innovation Promotion Association of the CAS (id. 2019060)
文摘Based on high resolution,high signal-to-noise(S/N)ratio spectra from Keck/HIRES,we have determined abundances of 20 elements for 18 Ba candidates.The parameter space of these stars is in the range of 4880≤Teff≤6050 K,2.56≤log g≤4.53 dex and-0.27≤[Fe/H]≤0.09 dex.It is found that four of them can be identified as Ba stars with[s/Fe]>0.25 dex(s:Sr,Y,Zr,Ba,La,Ce and Nd),and three of them are newly discovered,which include two Ba giants(HD 16178 and HD 22233)and one Ba subgiant(HD 2946).Our results show that the abundances ofα,odd and iron-peak elements(O,Na,Mg,Al,Si,Ca,Sc,Ti,Mn,Ni and Cu)for our program stars are similar to those of the thin disk,while the distribution of[hs/ls](hs:Ba,La,Ce and Nd,ls:Sr,Y and Zr)ratios of our Ba stars is similar to those of the known Ba objects.None of the four Ba stars show clear enhancement in carbon including the known CH subgiant HD 4395.It is found that three of the Ba stars present clear evidence of hosting stellar or sub-stellar companions from the radial velocity data.
基金supported by a Grant-in-Aid for Challenging Exploratory Research(No.15K12192)from the Japan Society for the Promotion of Science(JSPS)The State Scholarship Fund of Chinese Scholarship Council(CSC No.201406010350)provides Mr.Wei Hu the scholarship for his study and stay at the Prefectural University of Kumamoto
文摘Rainwater contains substantial bacteria and rain is an efficient pathway for the dissemination of bacteria from the atmosphere to land and water surfaces.However,quantitative information on rainwater bacteria is very limited due to the lack of a reliable method.In this study,the epifluorescence microscopy enumeration with the LIVE/DEAD BacLight Bacterial Viability Kit stain was verified to quantify the abundance of viable and non-viable bacterial cells in rainwater,with the 4＇,6-diamidino-2-phenylindole（DAPI） stain for the reference of total cell counts.Results showed that the total counts of bacterial cells by LIVE/DEAD BacLight staining were consistent with those by DAPI staining,and the average detection efficiency was（109 ± 29）%.The ratio of cell count with glutaraldehyde fixation to that without fixation was（106 ± 5）%on average.The bacterial concentration in negative control was usually an order of magnitude lower than that in rainwater samples.However,in case of small precipitation,the abundance in negative control could be more than that in rainwater samples.These results indicate that the enumeration with LIVE/DEAD BacLight bacterial viability assay coupled with glutaraldehyde fixation and careful negative control investigation is an approach applicable to the measurement of the concentration and viability of bacterial cells in rainwater.
文摘Low availability of phosphorus(P) is a major constraint for optimal crop production, as P is mostly present in its insoluble form in soil. Therefore,phosphate-solubilizing bacteria(PSB) from paddy field soils of the Indo-Gangetic Plain, India were isolated, and their abundance was attempted to be correlated with the physicochemical characteristics of the soils. Ninety-four PSB were isolated on Pikovskaya’s agar medium, and quantitative phosphate solubilization was evaluated using NBRIP medium. The isolates solubilized P up to a concentration of 1 006 μg mL-1 from tricalcium phosphate with the secretion of organic acids. These isolates were identified by 16 S rRNA gene sequence comparison, and they belonged to Gammaproteobacteria(56 isolates),Firmicutes(28 isolates), Actinobacteria(8 isolates), and Alphaproteobacteria(2 isolates). Phylogenetic analysis confirmed the identification by clustering the isolates in the clade of the respective reference organisms. The correlation analysis between PSB abundance and physicochemical characteristics revealed that the PSB population increased with increasing levels of soil organic carbon, insoluble P, K+, and Mg2+. The promising PSB explored in this study can be further evaluated for their biofertilizer potential in the field and for their use as potent bio-inoculants.
基金supported by the Program for the Development of Basic Sciences (Programa de Desarrollo de las Ciencias Básicas, PEDECIBA)provided by the Uruguayan Antarctic Institute (Instituto Antártico Uruguayo, IAU)
文摘Fildes Peninsula, in King George Island, Antarctica, has a great concentration of international facilities, and it has clearly been affected by human activities. The objective of this 5-year study was to assess the impact of anthropogenic activities on the bacterial abundance in water bodies close to Artigas Antarctic Scientific Base （BCAA, in Spanish Base Cientifica Antdrtica Artigas）. Water samples from areas under different human influence （Uruguay Lake, nearby ponds, and meltwater from Collins Glacier） were aseptically collected and refrigerated until processed. The number of heterotrophic bacteria and Pseudomonas spp. was analyzed using a culture-dependent approach. Physico-chemical properties of the water samples （temperature, pH, and conductivity） were also determined. Results showed that water from the highly affected area, Uruguay Lake, where the pump that provides water to the BCAA is located, did not suffer significant fluctuations in heterotrophie bacterial abundance （10^4- 10^5 CFU.mL^-1）; however, Pseudomonas abundance increased until becoming the predominant population. In other water samples, the number of heterotrophie bacteria and Pseudomonas gradually increased during this 5-year study, by 2014 reaching similar values to those observed for Uruguay Lake. The implications of human activities on Antarctic bacterial abundance are discussed.
基金the Innovative team project of Nanjing Institute of Environmental Sciences,MEE(GYZX200101)the National Natural Science Foundation of China(No.51778265)Key R&D Program of Ningxia Hui Autonomous Region(2019BFG02028,2021BEG01002).
文摘Fluoride(F^(-))is widespread in aquatic environments;however,it is not clear whether the fluctuation of F^(-)concentrations in overlying lake water affects the composition and migration of dissolved organic matter(DOM)from sediment.A case study was presented in Sand Lake,China,and an experiment was conducted to analyze the influence of different F^(-)concentrations in overlying water on DOM characteristics.Diffusion resulted in similarities in DOM components between overlying and pore waters,and bacterial activities and enzyme variation resulted in differences between them.Higher F^(-)concentrations in overlying water resulted in a higher pH of pore water,which favored the enrichment of protein-like substances.Higher F^(-)concentrations caused lower DOM concentrations and lower maximum fluorescence intensities(Fmax)of protein-like components in pore water.The F^(-)concentrations had significantly negative correlations with Shannon indexes(P<0.05).Thiobacillus influenced the migration of tyrosinelike substances by decreasing the pH of pore water.Trichococcus and Fusibacter altered the Fmax of protein-like,humic-like,and fulvic-like substances.The F^(-)concentrations affected the DOM composition and migration due to the response of functional bacterial communities,which were positively correlated with the relative abundance of Thiobacillus and negatively correlated with the relative abundances of Trichococcus and Fusibacter.The high F^(-)concentrations influenced the biosynthesis and degradation of protein-like substances by shifting the abundances of the relevant enzymes.The results of this study may provide ideas for investigating DOM cycling under the influence of F^(-),especially in lakes with fluctuations in F^(-)concentrations.
