Improving treatment plan and mental health in children with abdominal infection for broad-spectrum bacterial infections

BACKGROUND Pediatric abdominal infection is a common but serious disease that requires timely and effective treatment.In surgical treatment,accurate diagnosis and rational application of antibiotics are the keys to improving treatment effects.AIM To investigate the effect of broad-spectrum bacterial detection on postoperative antibiotic therapy.METHODS A total of 100 children with abdominal infection who received surgical treatment in our hospital from September 2020 to July 2021 were grouped.The observation group collected blood samples upon admission and sent them for broad-spectrum bacterial infection nucleic acid testing,and collected pus or exudate during the operation for bacterial culture and drug sensitivity testing;the control group only sent bacterial culture and drug sensitivity testing during the operation.RESULTS White blood cell count,C-reactive protein,procalcitonin,3 days after surgery,showed better postoperative index than the control group(P<0.05).The hospital stay in the observation group was significantly shorter than that in the control group.The hospitalization cost in the observation group was significantly lower than that in the control group,and the difference between the two groups was statistically significant(P<0.05).CONCLUSION Early detection of broad-spectrum bacterial infection nucleic acids in pediatric abdominal infections can help identify pathogens sooner and guide the appropriate use of antibiotics,improving treatment outcomes and reducing medical costs to some extent.

Antibacterial effect of povidone iodine disinfectant and antibiotic eye drops before cataract surgery

Objective:Investigate the distribution of bacteria in the conjunctival sac of two groups of cataract patients with different preoperative aseptic treatments,and compare the clinical effects of 0.5%povidone-iodine(PI)and antibiotic eye drops on killing conjunctival sac bacteria.Methods:400 cases of patients(400 eyes)who underwent cataract surgery in the Department of Ophthalmology of Baogang Hospital in Inner Mongolia from October 2019 to October 2020 were selected in a randomized controlled study.They were randomly divided into the experimental group(group A)and the control group(group B),with 200 cases in each group.Group A:the patients were given Gatifloxacin Eye Gel(3 times/day,1 drop/time)combined with Diclofenac Sodium Eye Drops(4 times/day,1 drop/time)before the operation;0.5%PI was administered to irrigate the conjunctival sac,with 0.9%Sodium Chloride Injection washing after 3 min;0.5%PI was administered to irrigate the conjunctival sac after operation.The corneal epithelium was observed after each irrigation.Group B:the patients were given Gatifloxacin Eye Gel(3 times/day,1 drop/time)combined with Diclofenac Sodium Eye Drops(4 times/day,1 drop/time)2 days and 1 day before the operation;0.9%Sodium Chloride Injection was administered to irrigate the conjunctival sac before and after surgical disinfection.Conjunctival sac specimens were collected for bacterial culture 2 hours before the operation,after irrigation,and after the procedure.The positive rate and the distribution of bacteria were compared between the two groups.Results:The difference in the positive rate of bacteria in the conjunctival sac between the two groups at different time points had a statistical significance(χ^(2)=11.498,p<.022).Conjunctival sac specimens were collected on admission and 2 hours before the operation.There was no significant difference in the pathogens with positive results between the two groups(p=.955;p=.073);there was a substantial difference in the distribution of positive pathogens between the two groups before and after surgical disinfection(p<.001);there was a significant difference in the distribution of pathogens between the two groups after the operation(p=.005).For Staphylococcus epidermidis,Corynebacterium,Enterococcus faecalis,Streptococcus,and other Gram-positive bacteria,there was a significant difference in the disinfection methods between the two groups at different time points(p<.001);for Staphylococcus aureus,Gram-positive cocci,and Gram-negative bacteria,there was no significant difference in the disinfection methods between the two groups at different time points(p=.113;p=.224;p=.146).There was no significant difference between the two groups with 0-10 and 101-1000 bacterial colonies at different time points(p=.370 and.071,respectively).When there were 11-100 bacterial colonies,there was a significant difference between the two groups at different time points(p<.001).There was no significant difference in corneal epithelial injury between the two groups at other time points(χ^(2)=4.133,p=.127).Conclusions:The combination of 0.5%PI disinfectant and antibiotic eye drops can effectively reduce the bacterial load of the conjunctival sac before operation.At the same time,it is safe and effective to irrigate the conjunctival sac with 0.5%PI disinfectant before the procedure.

Clinical Investigation on the Correlation between Lower Urinary Tract Infection and Cystitis Glandularis

Summary: In order to study the association between lower urinary tract infection and cystitis glandularis (CG), 120 cases of CG were diagnosed by cystoscopic biopsy in the suspicious foci of the bladder. Among them, 72 cases were subjected to bacterial counting culture of urine and microscopic examination of urinary sediment, and 60 cases to fluorescence quantitative polymerase chain reaction (FQ-PCR) assay to detect HPV, CMV and HSV DNA in urine samples. In the 72 cases of CG, the positive rate of bacterial counting culture of urine was 15.3 % (11/72), and gray zone rate was 18.1 % (13/72). 31.9 % (23/72) patients were positive in bacterioscopy of urinary sediment. There was statistically significant difference as compared with the control group (P<0.01). Only 4 of 60 urine samples were positive by FQ-PCR in detection of the three viruses mentioned above with the positive rate being 6.67 %. Compared with the control group, there was no significant difference (P>0.05). It was concluded that the genesis of CG was closely correlated with the chronic lower urinary tract infection, especially caused by Esch coli.

Microbial Community Structure of Casing Soil During Mushroom Growth

The culturable bacterial population and phospholipid fatty acid （PLFA） profile of casing soil were investigated at different mushroom （Agaricus bisporus） cropping stages. The change in soil bacterial PLFAs was always accompanied by a change in the soil eulturable bacterial population in the first flush. Comparatively higher culturable bacterial population and bacterial PLFAs were found in the casing soil at the primordia formation stage of the first flush. There was a significant increase in the ratio of fungal to bacterial PLFAs during mushroom growth. Multivariate analysis of PLFA data demonstrated that the mushroom cropping stage could considerably affect the microbial community structure of the casing soil. The bacterial population increased significantly from casing soil application to the primordia formation stage of the first flush. Casing soil application resulted in an increase in the ratio of gram-negative bacterial PLFAs to gram-positive bacterial PLFAs, suggesting that some gram-negative bacteria might play an important role in mushroom sporophore initiation.

High-level disinfection of gastrointestinal endoscope reprocessing

High level disinfection(HLD) of the gastrointestinal(GI)endoscope is not simply a slogan, but rather is a form of experimental monitoring-based medicine. By definition,GI endoscopy is a semicritical medical device. Hence,such medical devices require major quality assurance for disinfection. And because many of these items are temperature sensitive, low-temperature chemical methods, such as liquid chemical germicide, must be used rather than steam sterilization. In summarizing guidelines for infection prevention and control for GI endoscopy, there are three important steps that must be highlighted: manual washing, HLD with automated endoscope reprocessor, and drying. Strict adherence to current guidelines is required because compared to any other medical device, the GI endoscope is associated with more outbreaks linked to inadequate cleaning or disinfecting during HLD. Both experimental evaluation on the surveillance bacterial cultures and in-use clinical results have shown that, the monitoring of the stringent processes to prevent and control infection is an essential component of the broader strategy to ensure the delivery of safe endoscopy services, because endoscope reprocessing is a multistep procedure involving numerous factors that can interfere with its efficacy. Based on our years of experience in the surveillance of culture monitoring of endoscopic reprocessing, we aim in this study to carefully describe what details require attention in the GI endoscopy disinfection and to share our experience so that patients can be provided with high quality and safe medical practices. Quality management encompasses all aspects of pre- and postprocedural care including the efficiency of the endoscopy unit and reprocessing area, as well as the endoscopic procedure itself.

Moxa-Stick Suffumigation for Disinfecting Air in Hematology and Hematopoietic Stem Cell Transplantation Wards with Class 100 Laminar Flow

Objective： To evaluate the effect of moxa-stick suffumigation in the hematology and hematopoieticstem cell transplantation （HSCT） wards with luminar flow. Methods： The plate exposure method was usedto measure the effect of air-disinfection of moxa-stick suffumigation in hematology and HSCT wards. Theyearly average qualified rates of air sampling in HSCT wards were evaluated from 2007 to 2010. To furtherinvestigate the disinfecting effect of moxa-stick suffumigation, the colony counts of common pathogens（including Staphylcoccus aureus and Pseudomonas aeruginosa） before and after moxa-stick suffumigation werecompared. Results： The mean air quality rates of the HSCT wards with class 100 laminar flow were all above90.0% （91.2%-96.2%） from 2007 to 2010. Moxa-stick suffumigation effectively decreased the presence ofbacteria in the hematology ward＇s air （P〈0.01）. The most notable effect was the drastic reduction in the colonycounts of Staphylococcus aureus and Pseudomonas aeruginosa on the blood plates exposed to air treatedwith moxa-stick suffumigation （77.1 ± 52.9 cfu/m2 vs 196.1 ± 87.5 cfu/m2, P〈0.01; and 100.2±35.3 cfu/m2 vs371.5± 35.3 cfu/m2, P〈0.01）. Conclusion： Moxa-stick suffumigation proved to be a reliable and effective air-disinfection method for hematology and HSCT wards, and hence, it should be employed extensively.

Pyogenic Liver Abscess Caused by Staphylococcus hominis:A Case Report

Staphylococcus hominis is a component of the normal human microflora and is considered to be an opportunistic pathogen that may cause a variety of infections.It has been reported to cause bacteremia,endocarditis and endophthalmitis.However,abscesses caused by S.hominis are rarely documented.Here,we describe a case of pyogenic liver abscesses caused by S.hominis.Laboratory tests,imaging examinations,and abscess puncture fluid culture were used to identify the etiology and pathogenic bacterium.This case suggests that percutaneous drainage and targeted antibiotic treatment according to the results of antimicrobial susceptibility tests are effective therapeutic approaches for pyogenic liver abscesses caused by S.hominis.