Bacterial infection associated with the use of medical or dental devices is a serious concern.Although devices made of ethylene vinyl acetate（EVA） are often used in the oral cavity,there are no established standards...Bacterial infection associated with the use of medical or dental devices is a serious concern.Although devices made of ethylene vinyl acetate（EVA） are often used in the oral cavity,there are no established standards for their storage.We investigated bacterial survival on EVA sheets under various storage conditions to establish a standard for hygienic storage of such dental devices.Bacterial counts were evaluated,which showed a significant decrease after washing with sterilized water,mechanical brushing and rinsing,and using Mouthguard Cleaner as compared to untreated samples.In addition,no bacteria were detected on samples stored 2 days or longer in a ventilated environment,whereas they were detected for up to 14 days on samples without any cleaning stored in a closed environment.Bacterial counts for the untreated samples gradually declined,while surviving bacteria on samples treated with sterilized water and mechanical brushing showed a rapid decrease.Additionally,bacterial identification using polymerase chain reaction（PCR） revealed that Streptococcus oralis was dominantly detected on salivary samples after 14 days of storage among both two subjects.For effective hygienic storage of dental devices made of EVA,washing with sterilized water is important to remove absorbed salivary compounds along with storage in a ventilated environment.展开更多
Although bacterial persistence was first observed in 1944,its underlying mechanism is just beginning to be understood and many fundamental questions remain.In this review,we summarize studies in order to chart the ful...Although bacterial persistence was first observed in 1944,its underlying mechanism is just beginning to be understood and many fundamental questions remain.In this review,we summarize studies in order to chart the full map of bacterial persistence.Because persistence significantly contributes to disease recalcitrance,we also elucidate the probable relationships between bacterial persistence and prolonged chronic infections,with some comments on future research directions and therapeutic strategies.展开更多
文摘Bacterial infection associated with the use of medical or dental devices is a serious concern.Although devices made of ethylene vinyl acetate（EVA） are often used in the oral cavity,there are no established standards for their storage.We investigated bacterial survival on EVA sheets under various storage conditions to establish a standard for hygienic storage of such dental devices.Bacterial counts were evaluated,which showed a significant decrease after washing with sterilized water,mechanical brushing and rinsing,and using Mouthguard Cleaner as compared to untreated samples.In addition,no bacteria were detected on samples stored 2 days or longer in a ventilated environment,whereas they were detected for up to 14 days on samples without any cleaning stored in a closed environment.Bacterial counts for the untreated samples gradually declined,while surviving bacteria on samples treated with sterilized water and mechanical brushing showed a rapid decrease.Additionally,bacterial identification using polymerase chain reaction（PCR） revealed that Streptococcus oralis was dominantly detected on salivary samples after 14 days of storage among both two subjects.For effective hygienic storage of dental devices made of EVA,washing with sterilized water is important to remove absorbed salivary compounds along with storage in a ventilated environment.
基金supported by the National Natural Science Foundation of China(31370847)the Recruitment Program of Global Youth Experts
文摘Although bacterial persistence was first observed in 1944,its underlying mechanism is just beginning to be understood and many fundamental questions remain.In this review,we summarize studies in order to chart the full map of bacterial persistence.Because persistence significantly contributes to disease recalcitrance,we also elucidate the probable relationships between bacterial persistence and prolonged chronic infections,with some comments on future research directions and therapeutic strategies.
