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Bactericidal/permeability-increasing protein originates in both the testis and the epididymis and localizes in mouse spermatozoa 被引量:2
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作者 Zhong-Ping Zhou 《Asian Journal of Andrology》 SCIE CAS CSCD 2014年第2期309-313,I0012,共6页
Bactericidal/permeability-increasing protein (BPI) is an endogenous antibiotic protein with activity against gram-negative bacteria. In the present study, we examined the expression of BPI in postnatal mouse testes ... Bactericidal/permeability-increasing protein (BPI) is an endogenous antibiotic protein with activity against gram-negative bacteria. In the present study, we examined the expression of BPI in postnatal mouse testes and epididymides as well as the subcellular localization within epididymal spermatozoa. Our results showed that, BPI mRNA was expressed in testis and epididymis independently. Throughout the epididymis, the BPI protein level gradually decreased in the epididymal epithelium in a spatial manner, specialized within the cytoplasm of clear cells in the cauda part. We detected BPI proteins in intact acrosome, implying its testicular origin; on the other hand, after the acrosome reaction, BPI proteins were observed dispersed across the entire sperm head, especially enriched at the equatorial segment. Our findings suggested a dual origin of the BPI that generated both in the testis and epididymis, and associated with mouse spermatozoa. BPI protein might be involved in the dynamics modification of the sperm plasma membrane and also the fertilization process. 展开更多
关键词 ACROSOME antimicrobial protein bactericidal/permeability-increasing protein EPIDIDYMIS TESTIS
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Protective effects of bactericidal/permeability-increasing protein onrats after endotoxic shock
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作者 蒋建新 朱佩芳 +3 位作者 王正国 刘大维 何娅妮 谢国旗 《Journal of Medical Colleges of PLA(China)》 CAS 1999年第3期180-183,共4页
objective: To investigate the protective effects of bactericidal/permeability-increa protein (BPIP) on rats after endotoxic shock as to provide more experimental evidence for studies on its clinical use. Methods:E. co... objective: To investigate the protective effects of bactericidal/permeability-increa protein (BPIP) on rats after endotoxic shock as to provide more experimental evidence for studies on its clinical use. Methods:E. coli 026:B6 LPS was injected at a dosage of 12. 5 mg/kg through the artery to reproduce endo toxic shock. BPIP at a dosage of 5 mg/kg (BPIP-treated group) or equal volume of normal saline (control group) were injected immediately after the injection of LPS. Results: ①Survival time of the shocked animals was prolonged and the 24 h survival rate was also significantly increased in BPIP-treated group as compared with the control group. ②The mean arterial pressure, left intraventricular systolic pressure, isovolemic ven tricular pressure and ±dp/dtmax. were significantly higher in BPIP-treated group than in control group. ③ Plasma levels of glutamic-pyruvic transaminase and urea nitrogen were markedly higher but those of endotox in and TNFα were lower in BPIP-treated group than in control group. Conclusion: BPIP can exert significant protective effects on cardiac, hepatic and renal functions in rats after endotoxic shock, indicating that BPIP might be a good choice in treatment of sepsis/septic shock. 展开更多
关键词 ENDOTOXIC shock bactericidal/permeability-increasing protein PROTECTION
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Protective effects of bactericidal/permeability-increasing protein against hemorrhagic shock and its mechanism
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作者 蒋建新 朱佩芳 +3 位作者 王正国 陈惠孙 田昆化 刁有芳 《Journal of Medical Colleges of PLA(China)》 CAS 1998年第2期143-145,149,共4页
Effects of bactericidal/permeability-increa protein(BPI, 1. 5 and 3. 5 rug/kg iv at the beginning of shock and resuscitation respectively) on the outcome of hemorrhagic shock was investigated in rats. Itwas found that... Effects of bactericidal/permeability-increa protein(BPI, 1. 5 and 3. 5 rug/kg iv at the beginning of shock and resuscitation respectively) on the outcome of hemorrhagic shock was investigated in rats. Itwas found that BPI administration could improve hepatic and renal functions after hemorrhagic shock, andenhance the survival rate of the rats with shock (BPI group: 81 % vs physiological saline group: 44 %, P <0.05). In BPI group, the plasma endotoxin level was not significantly changed (0. 20± 0. 04 at the end of resuscitation vs 0. 24± 0. 05 U/ml before shock). Tumor necrosis factor. and interleukin-6 levels in BPI group, although higher after shock and resuscitation, were significantly lower than those in physiologia saline group.It is suggested that BPI exerts a protective effects on rats with hernorrhagic shock, which might be due to itsaction against hemorrhage-induced endotoxin translocation and its inhibition of cytokine responses in shock, 展开更多
关键词 hernorrhagic/shock bactericidal/permeability-increa protein ENDOTOXIN tumor NECROSIS factor α INTERLEUKIN-6 rat
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Effect of tight junction protein of intestinal epithelium and permeability of colonic mucosa in pathogenesis of injured colonic barrier during chronic recovery stage of rats with inflammatory bowel disease 被引量:3
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作者 Chun-Mei Xu Xiu-Mei Li +1 位作者 Bing-Zhao Qin Bo Liu 《Asian Pacific Journal of Tropical Medicine》 SCIE CAS 2016年第2期143-147,共5页
Objective: To discuss the changes in the tight junction protein of intestinal epithelium and permeability of colonic mucosa and its possible mechanism by building the rat mode of inflammatory bowel disease at the chro... Objective: To discuss the changes in the tight junction protein of intestinal epithelium and permeability of colonic mucosa and its possible mechanism by building the rat mode of inflammatory bowel disease at the chronic recovery stage. Methods: A total of 36 SD rats were divided into the model group and control one according to the random number table, with 18 rats in each group. Rats in the model group were given the 3% dextran sulfate sodium solution by the way of drinking for 7 d to build the rat model of inflammatory bowel disease, while rats in the control group were given free drinking of water. Six rats were executed at day 7, 14 and 21 respectively. The colonic tissues were collected from rats to observe the pathological changes of colonic mucosa. The activity of myeloperoxidase was detected and the white blood count was performed for rats in each group. The Ussing chamber technique was employed to detect the transepithelial electrical resistance(TER) and short-circuit current(SC) of colonic mucosa of rats in different time intervals; the quantum dots labeling technique was employed to detect the expression level of claudin-1 and claudin-2 in the colonic tissues. Results: After the successful modeling, the weight of rats in the model group was significantly reduced, while the disease activity index score was increased. The weight was at the lowest level at day 14 and then it began to increase afterwards. The disease activity index score was at the highest level at day 12 and then it began to decrease gradually. The activity of myeloperoxidase and WBC for rats in the model group all reached the peak value at day 14 and then decreased gradually. There was no significant difference in the changes of TER and SC in different time intervals for rats in the control group(P>0.05). TER of model group was at the lowest level at day 14 and then increased gradually; SC was at the highest level at day 14 and then decreased gradually. TER of model group at day 7, 14 and 21 was significantly lower than that of control group, while SC of model group was significantly higher than that of control group(P<0.05). There was no significant difference in the change of mean fluorescence intensity of claudin-1 and claudin-2 in different time intervals for rats in the control group(P>0.05). The claudin-1 and claudin-2 for rats in the model group reached the highest level at day 14 and then decreased gradually. The claudin-1 and claudin-2 of model group at day 7, 14 and 21 was significantly higher than that of control group(P<0.05). Conclusions: After the acute stage, the inflammatory bowel disease is then in the chronic recovery stage; the increased permeability of colonic mucosa and increased expression of tight junction protein of intestinal epithelium are closely related to the pathogenesis and development of disease. The tight junction protein plays a key role in the pathogenesis of injured colonic barrier of inflammatory bowel disease. 展开更多
关键词 Inflammatory BOWEL disease Tight junction protein COLONIC MUCOSA permeability COLONIC BARRIER
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Relationship of gelatinases-tight junction proteins and blood-brain barrier permeability in the early stage of cerebral ischemia and reperfusion 被引量:4
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作者 Haolin Xin Wenzhao Liang +4 位作者 Jing Mang Lina Lin Na Guo Feng Zhang Zhongxin Xu 《Neural Regeneration Research》 SCIE CAS CSCD 2012年第31期2405-2412,共8页
Gelatinases matrix metalloproteinase-2 and matrix metalloproteinase-9 have been shown to mediate claudin-5 and occludin degradation, and play an important regulatory role in blood-brain barrier permeability. This stud... Gelatinases matrix metalloproteinase-2 and matrix metalloproteinase-9 have been shown to mediate claudin-5 and occludin degradation, and play an important regulatory role in blood-brain barrier permeability. This study established a rat model of 1.5-hour middle cerebral artery occlusion with reperfusion. Protein expression levels of claudin-5 and occludin gradually decreased in the early stage of reperfusion, which corresponded to the increase of the gelatinolytic activity of matrix metalloproteinase-2 and matrix metalloproteinase-9. In addition, rats that received treatment with matrix metalloproteinase inhibitor N-[(2R)-2-(hydroxamidocarbonylmethyl)-4-methylpenthanoyl]-L- tryptophan methylamide (GM6001) showed a significant reduction in Evans blue leakage and an inhibition of claudin-5 and occludin protein degradation in striatal tissue. These data indicate that matrix metalloproteinase-2 and matrix metalloproteinase-9-mediated claudin-5 and occludin degradation is an important reason for blood-brain barrier leakage in the early stage of reperfusion. The leakage of the blood-brain barrier was present due to gelatinases-mediated degradation of claudin-5 and occludin proteins. We hypothesized that the timely closure of the structural component of the blood-brain barrier (tight junction proteins) is of importance. 展开更多
关键词 gelatinases matrix metalloproteinase claudin-5 occludin blood-brain barrier Evans blue middle cerebral artery occlusion reperfusion injury GM6001 junction protein permeability neural regeneration
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AB004. Regulation of retinal angiogenesis and vascular permeability by bone morphogenetic protein signaling
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作者 Bruno Larrivée 《Annals of Eye Science》 2018年第1期410-410,共1页
The bone morphogenetic protein(BMP)family of proteins has a multitude of roles throughout the body.It plays important roles in development and in the adult vascular endothelium,by modulating the angiogenic response.Th... The bone morphogenetic protein(BMP)family of proteins has a multitude of roles throughout the body.It plays important roles in development and in the adult vascular endothelium,by modulating the angiogenic response.The endothelial-specific receptor BMP receptor Alk1 is of particular importance in the proper remodeling of the vasculature and its ligand BMP9 has been shown to be a potent inhibitor of neovascularization.Dysregulated BMP signaling has been linked to multiple vascular diseases and can lead to the abnormal angiogenesis.We therefore investigated the role of BMP9/Alk1 signaling in retinal angiogenesis,and its therapeutic implications for vascular pathologies of the eye. 展开更多
关键词 Retinal angiogenesis bone morphogenetic protein(BMP) vascular permeability
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C-reactive protein aggravates myocardial ischemia/reperfusion injury through activation of extracellular-signal-regulated kinase 1/2 被引量:10
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作者 Wei-Na PEI Hai-Juan HU +3 位作者 Fan LIU Bing XIAO Ya-Bei ZUO Wei CUI 《Journal of Geriatric Cardiology》 SCIE CAS CSCD 2018年第7期502-513,共12页
Background Ischemia/reperfusion injury (IRI) is an inflammatory response that occurs when tissue is reperfused following a prolonged period of ischemia. Several studies have indicated that C-reactive protein (CRP)... Background Ischemia/reperfusion injury (IRI) is an inflammatory response that occurs when tissue is reperfused following a prolonged period of ischemia. Several studies have indicated that C-reactive protein (CRP) might play an important role in inducing IRI. However, the effects of CRP on myocardial IRI and the underlying mechanisms have not been fully elucidated. This study aimed to investigate the association between CRP and myocardial IRI and the underlying mechanisms. Methods We simulated ischemia/reperfusion using oxygen-glucose deprivation/ reoxygenation (OGD/R) in neonatal Sprague-Dawley rat cardiomyocytes; reperfusion injury was induced by three hours of hypoxia with glucose and serum deprivation followed by one hour of reperfusion. Cell viability was tested with MTS assays, and cardiomyocyte damage was evaluated by lactate dehydrogenase (LDH) leakage. Mitochondrial membrane potential was measured using tetramethylrhodamine ethyl ester (TMRE) and mitochondrial permeability transition pore (mPTP) opening was measured using calcein/AM; both TMRE and caocein/AM were visualized with laser scanning confocal microscopy. In addition, we studied the signaling pathways underlying CRP-mediated ischemia/reperfusion injury via Western blot analysis. Results Compared with the simple OGD/R group, after intervention with 10 pg/mL CRP, cell viability decreased markedly (82.36 % ± 6.18% vs. 64.84% ± 4.06%, P = 0.0007), and the LDH leakage significantly increased (145.3 U/L ± 16.06 U/L vs. 208.2 U/L ± 19.23 U/L, P = 0.0122). CRP also activated mPTP opening and reduced mitochondrial membrane potential during myocardial ischemia/reperfusion. Pretreatment with 1 pM atorvastatin (Ator) before CRP intervention protected cardiomyocytes from IRI. Mitochondrial KATP channel opener diazoxide and mPTP inhibitor cyclosporin A also offset the effects of CRP in this process. The level of phosphorylated extracellular-signal-regulated kinase (ERK) 1/2 was significantly higher after pre-treatment with CRP compared with the OGD/R group (170.4% ± 3.00% v.v. 93.53% ± 1.94%, P 〈 0.0001). Western blot analysis revealed that Akt expression was markedly activated (184.2% ± 6.96% vs. 122.7% ± 5.30%, P = 0.0003) and ERK 1/2 phosphorylation significantly reduced after co-treatment with Ator and CRP compared with the level after CRP pretreatment alone. Conclusions Our results suggested that CRP directly aggravates myocardial IRI in myocardial cells and that this effect is primarily mediated by inhibiting mitochondrial ATP- sensitive potassium (mitoKATp) channels and promoting mPTP opening. Ator counteracts these effects and can reduce CRP-induced IRI. One of the mechanisms of CRP-induced IRI may be related to the sustained activation of the ERK signaling pathway. 展开更多
关键词 C-reactive protein Ischemia/reperfusion injury Mitochondrial permeability transition pore Mitochondrial KATP channel STATIN
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小白链霉菌全细胞转化L-赖氨酸合成ε-聚赖氨酸的体系构建与优化
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作者 朱道君 刁文娇 +4 位作者 张佳微 王靓 张宏建 张建华 陈旭升 《食品与发酵工业》 CSCD 北大核心 2024年第1期29-36,共8页
小白链霉菌(Streptomyces albulus)是天然抗菌肽ε-聚赖氨酸(ε-poly-L-lysine,ε-PL)的主要生产菌株。为了提高小白链霉菌生产ε-PL效率,该文构建并优化了全细胞转化L-赖氨酸合成ε-PL体系:葡萄糖质量浓度80 g/L,菌龄12 h,反应温度30℃... 小白链霉菌(Streptomyces albulus)是天然抗菌肽ε-聚赖氨酸(ε-poly-L-lysine,ε-PL)的主要生产菌株。为了提高小白链霉菌生产ε-PL效率,该文构建并优化了全细胞转化L-赖氨酸合成ε-PL体系:葡萄糖质量浓度80 g/L,菌龄12 h,反应温度30℃,L-赖氨酸质量浓度15 g/L,柠檬酸浓度15 g/L,初始反应p H 4.0,硫酸铵质量浓度6 g/L,湿菌体量为1900 g/L。基于该转化体系,实现小白链霉菌在96 h合成ε-PL产量和底物转化率达到13.80 g/L和38.9%,分别是常规摇瓶发酵的4.1、3.2倍。最后,在小白链霉菌中异源表达来自大肠杆菌的L-赖氨酸特异性通透蛋白基因lysp,获得的重组菌S.albulus OE-lysp实现L-赖氨酸利用能力和底物转化率较出发菌株分别提升26%和33%,ε-PL产量增加至17.21 g/L,约为常规摇瓶发酵ε-PL产量的6.4倍,这是文献报道的最高摇瓶规模ε-PL产量。该研究结果一方面说明了通过全细胞转化L-赖氨酸生产ε-PL的可行性,另一方面为S.albulus转化大宗氨基酸L-赖氨酸生产高值ε-PL奠定了坚实的技术基础,具有重要的理论意义和经济价值。 展开更多
关键词 小白链霉菌 Ε-聚赖氨酸 全细胞转化 异源表达 L-赖氨酸通透蛋白
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谷朊粉对大豆分离蛋白膜理化性质的影响
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作者 江睿钊 石林凡 +3 位作者 任中阳 杨燊 张玉苍 翁武银 《中国粮油学报》 CAS CSCD 北大核心 2024年第5期79-85,共7页
为利用枧水处理的谷朊粉(WG)改良大豆分离蛋白(SPI)膜性质,考察了m_(WG)∶m_(SPI)分别为0∶10、1∶9、2∶8、3∶7、4∶6时对复合膜理化性质的影响,测定膜的微观结构、蛋白结构、机械性能等理化性质。扫描电子显微镜结果显示添加的WG主... 为利用枧水处理的谷朊粉(WG)改良大豆分离蛋白(SPI)膜性质,考察了m_(WG)∶m_(SPI)分别为0∶10、1∶9、2∶8、3∶7、4∶6时对复合膜理化性质的影响,测定膜的微观结构、蛋白结构、机械性能等理化性质。扫描电子显微镜结果显示添加的WG主要聚集在复合膜下表面,当m_(WG)∶m_(SPI)超过2∶8时膜的下表面网络结构会遭到破坏。根据傅里叶变换红外光谱和十二烷基硫酸钠-聚丙烯酰氨凝胶电泳的结果,发现WG会阻碍SPI分子之间的交联。SPI膜的抗拉伸强度和断裂伸长率分别为6.60 MPa和54.91%,伴随WG的添加抗拉伸强度逐渐下降而断裂伸长率逐渐上升。复合膜的水蒸气阻隔能力和上表面接触角随着WG比例的增加而增大。当m_(WG)∶m_(SPI)增加到2∶8时,WG/SPI复合膜的b*值达到了18.72的高值。差示扫描量热法的结果表明,在m_(WG)∶m_(SPI)为1∶9时,复合膜的热稳定性最高。研究结果表明适当添加枧水预处理的WG能提高SPI膜的部分物理性质,这将为SPI膜理化性质改良提供新的途径。 展开更多
关键词 大豆分离蛋白 谷朊粉 枧水 复合膜 水蒸气透过率
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肝硬化患者血清高迁移率族蛋白B水平变化及其与肠黏膜通透性的相关性
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作者 谢娇娇 何燕芳 +2 位作者 马燕花 郭才 郑兰兰 《山东医药》 CAS 2024年第15期9-12,共4页
目的观察不同病情肝硬化患者血清高迁移率族蛋白B1(HMGB1)、肠黏膜通透性(IP)相关指标[D-乳酸(D-lac)、二胺氧化酶(DAO)、内毒素(ET)]水平变化,分析血清HMGB1水平与IP相关指标的相关性,以明确血清HMGB1水平与患者IP的关系。方法肝硬化患... 目的观察不同病情肝硬化患者血清高迁移率族蛋白B1(HMGB1)、肠黏膜通透性(IP)相关指标[D-乳酸(D-lac)、二胺氧化酶(DAO)、内毒素(ET)]水平变化,分析血清HMGB1水平与IP相关指标的相关性,以明确血清HMGB1水平与患者IP的关系。方法肝硬化患者63例,根据Child-Pugh评分分为3组,CP-A级组16例、CP-B级组32例、CP-C级组15例,同期收集体检健康者33例作为对照组。采用ELISA法检测各组血清HMGB1、ET、DAO、D-lac。分析肝硬化患者血清HMGB1与IP相关指标的相关性及血清HMGB1、IP相关指标与Child-Pugh分级的相关性。结果CP-A级、CP-B级、CP-C级组血清HMGB1、ET、DAO、D-lac水平均高于对照组,CP-C级组血清HMGB1、D-lac、ET水平高于CP-A级组,CP-B级组血清HMGB1、ET水平高于CP-A级组(P均<0.05)。肝硬化患者血清HMGB1与IP相关指标ET、DAO、D-lac呈正相关(r分别为0.371、0.854、0.623,P均<0.05);血清HMGB1、ET、DAO、D-lac水平均与Child-Pugh分级呈正相关(r分别为0.356、0.272、0.301、0.382,P均<0.05)。结论肝硬化患者血清HMGB1水平升高,IP增加,且随着病情程度加重而改变更明显;血清HMGB1水平与患者IP有正相关关系。 展开更多
关键词 高迁移率族蛋白B1 肠黏膜通透性 D-乳酸 二胺氧化酶 内毒素 肝硬化
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肝素结合蛋白对脓毒症患者血管通透性影响的研究
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作者 朱秀琪 《中国现代医生》 2024年第10期32-35,共4页
目的 探讨肝素结合蛋白(heparin-bindingprotein,HBP)与脓毒症患者血管通透性的相关性。方法 回顾性选取2019年11月至2022年11月浙江大学医学院附属金华医院收治的400例感染患者,根据是否诊断为脓毒症将其分为脓毒症组(n=190)和非脓毒症... 目的 探讨肝素结合蛋白(heparin-bindingprotein,HBP)与脓毒症患者血管通透性的相关性。方法 回顾性选取2019年11月至2022年11月浙江大学医学院附属金华医院收治的400例感染患者,根据是否诊断为脓毒症将其分为脓毒症组(n=190)和非脓毒症组(n=210)。比较两组患者的HBP、血细胞比容(hematocrit,HCT)与血清白蛋白(albumin,ALB)差值(HCT-ALB)及液体超负荷百分比。采用Pearson法分析HBP与HCT-ALB、液体超负荷百分比的相关性。结果 脓毒症组患者的HBP、HCT-ALB及液体超负荷百分比均显著高于非脓毒症组(P<0.05);Pearson相关性分析结果显示,入院后24h血清HBP水平与HCT-ALB及液体超负荷百分比均呈正相关(P<0.05)。结论 脓毒症患者的血管通透性增加,可能与细菌毒素刺激中性粒细胞释放高水平HBP有关。 展开更多
关键词 脓毒症 肝素结合蛋白 血管通透性 液体超负荷
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IgG Fab-BPI融合蛋白真核表达载体的构建及其在CHO细胞中的表达 被引量:7
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作者 马越云 马文煜 +3 位作者 于文彬 尹文 苏明权 丁振若 《细胞与分子免疫学杂志》 CAS CSCD 北大核心 2001年第5期470-472,共3页
目的构建抗抗LPSFab-BPI真核表达载体,并在CHO细胞中表达。方法通过RT-PCR,获得广谱抗LPSmAbC3A2IgG的Fd和完整轻链LCcDNA片段。在分别构建了pcDNA3-Fd和pcDNA3-LC表达载体的基础上,将包括BPIN端抗LPS活性中心的180bpDNA片段,通过一段... 目的构建抗抗LPSFab-BPI真核表达载体,并在CHO细胞中表达。方法通过RT-PCR,获得广谱抗LPSmAbC3A2IgG的Fd和完整轻链LCcDNA片段。在分别构建了pcDNA3-Fd和pcDNA3-LC表达载体的基础上,将包括BPIN端抗LPS活性中心的180bpDNA片段,通过一段长16个氨基酸的linker连接于上述Fab表达载体中Fd基因的下游,构建成Fab-BPI融合蛋白FB真核表达载体。将pcDNA3-LC质粒中包括hCMV启动子、LC和BGHpolyA等序列在内的片段,插入到pcDNA3-Fd中hCMV启动子上游,从而构建成单质粒的Fab-BPI表达载体pcD-NA3-FB,转染真核细胞。结果序列测定表明,重组质粒构建正确。pcDNA3-FB转染CHO细胞后,经ELISA、免疫荧光和mR-NA鉴定表达成功,免疫印迹试验显示,与抗κ轻链和抗BPI抗体反应的蛋白区带的Mr均为60000左右。交叉反应试验证明,Fab和FB与多种革兰氏阴性菌的LPS有交叉反应性。结论成功地在CHO细胞中表达出了抗LPSFab-BPIFB融合蛋白。FB作为融合蛋白,集广泛交叉反应性和强大中和活性于一身,有可能成为更适于临床应用的新的抗LPS免疫制剂。 展开更多
关键词 LPS FAB 杀菌通透性增强蛋白 真核表达 IGG
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鉴别诊断临床急性肺炎病原体感染的血清标志物研究 被引量:12
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作者 刘萌 窦云鹏 +5 位作者 谷丽 吕喆 王一民 刘颖梅 安云庆 曹彬 《首都医科大学学报》 CAS 北大核心 2016年第5期636-640,共5页
目的检测急性肺炎患者血清相关生物标志物含量,为建立一种快速鉴别诊断临床急性支原体肺炎、病毒性肺炎、细菌性肺炎的免疫胶体金检测法提供实验依据。方法以临床病原学诊断明确的急性支原体肺炎,病毒性肺炎,细菌性肺炎患者和正常人血... 目的检测急性肺炎患者血清相关生物标志物含量,为建立一种快速鉴别诊断临床急性支原体肺炎、病毒性肺炎、细菌性肺炎的免疫胶体金检测法提供实验依据。方法以临床病原学诊断明确的急性支原体肺炎,病毒性肺炎,细菌性肺炎患者和正常人血清为检测样品,采用血小板衍生生长因子(platelet-derived growth factor,PDGF)/杀菌渗透增强蛋白(bactericidal/permeability-increasing protein,BPI)酶联免疫法(enzyme-linked immunosorbent assay,ELISA)检测试剂盒检测上述血清中相关生物标志物含量。结果 (1)正常人群血清78份,急性支原体肺炎患者血清46份,病毒性肺炎和细菌性肺炎患者血清42份,其中病毒感者患者血清35份,细菌感染患者血清7份;(2)正常人群血清PDGF最大值<6 000 pg/m L;血清BPI最大值<80 ng/m L;(3)以血清PDGF值6 000 pg/m L作为鉴别诊断急性支原体感染的基线值,支原体感染患者阳性检出率为93.47%(43/46);病毒和细菌感染患者出现支原体感染的假阳性率为7.14%(3/42);(4)以血清BPI值80 ng/m L作为鉴别诊断急性细菌感染的基线值,细菌感染患者阳性检出率为85.71%(6/7);病毒感染患者出现细菌感染的假阳性率为3.22%(1/31)。结论以血清PDGF6 000 pg/m L和BPI 80 ng/m L作为鉴别诊断不同病原体感染的基线值,对临床急性支原体肺炎、病毒性肺炎、细菌性肺炎鉴别诊断具有一定意义。 展开更多
关键词 肺炎 鉴别诊断 血小板衍生生长因子 杀菌渗透增强蛋白
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重组BPI_(23)-Fcγ1融合蛋白在CHO细胞中的表达 被引量:6
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作者 徐俊杰 徐静 +1 位作者 童贻刚 王海涛 《生物工程学报》 CAS CSCD 北大核心 2001年第5期587-589,共3页
The fusion gene of BPI 23 and human Fcγ1 was obtained by PCR method,and the expression plasmid was constructed to express recombinant BPI 23 \|Fcγ1 fusion protein in CHO cells.After transfection with the plasmid and... The fusion gene of BPI 23 and human Fcγ1 was obtained by PCR method,and the expression plasmid was constructed to express recombinant BPI 23 \|Fcγ1 fusion protein in CHO cells.After transfection with the plasmid and selection by methotrexate,the cell lines expressing the fusion protein were obtained.The recombinant protein was purified using cation\|exchange chromatography and its bioactivity was proved with bactericidal assays. 展开更多
关键词 杀菌/通透性增加蛋白 BPI23-Fcγ1融合蛋白 表达 CHO细胞 重组
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杀菌/通透性增加蛋白N端片段在原核细胞中的表达及其临床应用的初步探讨 被引量:4
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作者 周宇麒 张天托 +2 位作者 胡波 李林 黄静 《中国免疫学杂志》 CAS CSCD 北大核心 2005年第5期385-389,共5页
目的:构建编码杀菌/通透性增加蛋白(BPI)N端片段基因原核表达载体,并在大肠杆菌中表达重组蛋白。方法:采用RT PCR技术,从人外周血中性粒细胞的总RNA中扩增BPI蛋白N端片段cDNA编码区基因,DNA序列分析鉴定;将测序证实的BPI蛋白N端片段编... 目的:构建编码杀菌/通透性增加蛋白(BPI)N端片段基因原核表达载体,并在大肠杆菌中表达重组蛋白。方法:采用RT PCR技术,从人外周血中性粒细胞的总RNA中扩增BPI蛋白N端片段cDNA编码区基因,DNA序列分析鉴定;将测序证实的BPI蛋白N端片段编码区基因PCR产物直接克隆于原核表达载体pBAD/Thio TOPO中,再次测序鉴定;通过在大肠杆菌中以L 阿拉伯糖进行诱导表达,然后以SDS PAGE、Westernblot对表达的重组蛋白进行分析。重组蛋白经初步纯化处理后,进行生物学活性检测。结果:RT PCR扩增出一个835bp的DNA片段,DNA测序表明为所需目的基因。限制性内切酶酶切和DNA序列分析表明,BPI蛋白N端片段正确克隆到大肠杆菌表达载体pBAD/Thio TOPO中,SDS PAGE和Westerblot结果表明在大肠杆菌中成功表达了BPI蛋白N端片段。对该蛋白进行初步活性测定显示其可与BPI单克隆抗体结合并具有杀灭耐药铜绿假单胞菌的活性。结论:成功构建了BPI蛋白N端片段编码区基因原核表达载体,并在大肠杆菌中进行了表达。 展开更多
关键词 杀菌作用 通透性增加蛋白 基因克隆 基因表达 中性粒细胞
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BPI-BD3融合抗菌肽修饰C3H10T1/2细胞对小鼠感染创面愈合的影响 被引量:6
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作者 张欣然 刘翠 +3 位作者 钱智勇 徐红梅 郭希民 郭红延 《解放军医学杂志》 CAS CSCD 北大核心 2015年第9期722-726,共5页
目的观察p Adxsi-BPI-BD3转染C3H10T1/2细胞对小鼠感染创面愈合的影响。方法使用重组腺病毒载体p Adxsi-BPI-BD3转染C3H10T1/2细胞,并采用RT-PCR及Western blotting进行鉴定。取30只KM小鼠行直径1cm的全层皮肤缺损创面,并接种金黄色葡... 目的观察p Adxsi-BPI-BD3转染C3H10T1/2细胞对小鼠感染创面愈合的影响。方法使用重组腺病毒载体p Adxsi-BPI-BD3转染C3H10T1/2细胞,并采用RT-PCR及Western blotting进行鉴定。取30只KM小鼠行直径1cm的全层皮肤缺损创面,并接种金黄色葡萄球菌制造感染创面。造模后,随机均分为3组每组10只,T组尾静脉注射p Adxsi-BPIBD3转染后的C3H10T1/2细胞,C组注射未转染的C3H10T1/2细胞,N组注射等量PBS。通过大体观察、痂下细菌计数、测定创面残留面积、HE染色等来评价BPI-BD3修饰的C3H10T1/2的促愈效果。结果与其余两组相比,T组痂下菌量较少(P<0.05),且T组愈合较快,7、14d时,创面残留面积与其余两组相比差异有统计学意义(P<0.05)。HE染色结果显示,与其余两组相比,T组炎症较轻、表皮生长较快。结论 BPI-BD3修饰的C3H10T1/2可以促进金黄色葡萄球菌所致的小鼠感染创面的愈合。 展开更多
关键词 C3H10T1/2细胞 Β防御素 杀菌/通透性增加蛋白 伤口感染
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酸性氧化电位水对铜绿假单胞菌的杀菌机制研究 被引量:9
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作者 赵凯丽 李武平 +2 位作者 张晓娜 王刚 庄玉晨 《中国感染控制杂志》 CAS 北大核心 2017年第1期41-45,共5页
目的探讨酸性氧化电位水(EOW)对铜绿假单胞菌的杀菌机制。方法以铜绿假单胞菌作为研究对象,从蛋白质渗漏,核酸和细胞膜钙离子通透性等方面对EOW的杀菌机制进行研究,同时以2%戊二醛作为阳性对照组,以生理盐水(NS)作为阴性对照组。结果以... 目的探讨酸性氧化电位水(EOW)对铜绿假单胞菌的杀菌机制。方法以铜绿假单胞菌作为研究对象,从蛋白质渗漏,核酸和细胞膜钙离子通透性等方面对EOW的杀菌机制进行研究,同时以2%戊二醛作为阳性对照组,以生理盐水(NS)作为阴性对照组。结果以EOW、2%戊二醛对铜绿假单胞菌作用30 s杀灭率>99.99%,作用60 s杀灭率可达100.00%。EOW、NS、2%戊二醛作用铜绿假单胞菌60 s后,经BCA法检测蛋白渗漏,分别为(96.00±7.42)、(94.15±7.49)、(216.97±10.35)μg/mL,总体比较,差异有统计学意义(F=613.20,P<0.01),2%戊二醛组高于EOW组、NS组;蛋白渗漏量不随作用时间增加而改变(均P>0.05)。随机多态性扩增DNA电泳图谱显示,EOW组与NS组均在500~1 000 kb间显示亮度较高的密集条带,2%戊二醛组无扩增条带。EOW及2%戊二醛作用后钙离子荧光强度均低于NS组。结论 EOW可能的杀菌机制是使铜绿假单胞菌细胞膜通透性受到一定程度的损伤,致使Ca2+泄漏,但未能造成蛋白质泄漏,对其核酸损伤作用不明显,DNA可能并非EOW杀菌靶点。 展开更多
关键词 酸性氧化电位水 戊二醛 铜绿假单胞菌 杀菌机制 蛋白质泄漏 核酸 细胞膜通透性
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杀菌/渗透性增强蛋白功能及应用前景 被引量:4
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作者 颜金鹏 王丽 +2 位作者 李善妮 邓丽明 韩旭 《中国生物化学与分子生物学报》 CAS CSCD 北大核心 2011年第7期622-631,共10页
杀菌/通透性增强蛋白(BPI)是存在于中性粒细胞中的阳离子蛋白,约为55 kD.BPI能与革兰氏阴性菌外膜上的脂多糖结合,增加外膜对抗菌药的通透性,具有特异性杀灭革兰氏阴性菌及结合/中和内毒素的生物学功能,在革兰氏阴性菌感染的治疗方面有... 杀菌/通透性增强蛋白(BPI)是存在于中性粒细胞中的阳离子蛋白,约为55 kD.BPI能与革兰氏阴性菌外膜上的脂多糖结合,增加外膜对抗菌药的通透性,具有特异性杀灭革兰氏阴性菌及结合/中和内毒素的生物学功能,在革兰氏阴性菌感染的治疗方面有良好的发展前景.近年来,国内外对BPI研究颇多,并逐渐发现BPI还具有调理作用、抗真菌、抗原虫和抑制血管生成等许多功能,被学者称为未来的"超级抗生素".本文主要就BPI的结构、分布、生理功能、作用机理及临床研究方面的研究进展作一综述. 展开更多
关键词 杀菌/渗透性增强蛋白(BPI) 中性粒细胞 革兰氏阴性菌 内毒素
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杀菌/通透性增加蛋白研究现状及应用前景 被引量:4
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作者 李彦兵 桂祎 +3 位作者 李进涛 李作生 史宪伟 张以芳 《中国畜牧兽医》 CAS 北大核心 2009年第9期29-34,共6页
杀菌/通透性增加蛋白(BPI)是中性粒细胞内存在的一种碱性蛋白,是一个分子质量介于50-60 ku的阳性蛋白,它主要存在于人、牛、兔等哺乳动物多形核粒细胞(PN4NL)的嗜天青颗粒中及其表面,它能与革兰氏阴性菌脂多糖(LPS)结合,具有中和... 杀菌/通透性增加蛋白(BPI)是中性粒细胞内存在的一种碱性蛋白,是一个分子质量介于50-60 ku的阳性蛋白,它主要存在于人、牛、兔等哺乳动物多形核粒细胞(PN4NL)的嗜天青颗粒中及其表面,它能与革兰氏阴性菌脂多糖(LPS)结合,具有中和内毒素和杀灭细菌的作用,在革兰氏阴性菌感染的治疗方面有良好的应用前景,人类最初注意杀菌性/通透性增加蛋白是因其具有杀菌作用,但随后发现它还具有较强的颉颃内毒素的作用。近年来国外对其研究颇多,并逐渐发现其还具有很多其他功能,如抗真菌、杀灭寄生虫等作用,其被学者称为未来的“超级抗生素”。 展开更多
关键词 杀菌/通透性增加蛋白 内毒素 中性粒细胞
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BPI蛋白对感染绵羊肺炎支原体的盘羊杂交羊细胞因子水平的影响 被引量:4
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作者 郭海英 沈文 +4 位作者 陈冬梅 陈凯丽 张晓丽 高宝德 孙延鸣 《畜牧兽医学报》 CAS CSCD 北大核心 2015年第10期1882-1890,共9页
研究重组BPI蛋白(rBPI)对感染绵羊肺炎支原体(MO)的盘羊杂交羊的免疫应答分析。将6只巴什拜羊作为A组,将12只盘羊杂交羊随机分为B、C组,全部人工感染MO,感染第4天开始,C组每天分别注射rBPI,A和B组注射生理盐水。采用ELISA方法检测试验... 研究重组BPI蛋白(rBPI)对感染绵羊肺炎支原体(MO)的盘羊杂交羊的免疫应答分析。将6只巴什拜羊作为A组,将12只盘羊杂交羊随机分为B、C组,全部人工感染MO,感染第4天开始,C组每天分别注射rBPI,A和B组注射生理盐水。采用ELISA方法检测试验羊不同时间血清中IL-8、IL-10、IFN-γ及TNF-α的含量。在试验结束后宰杀各组6只羊,取肺组织做病理切片,进行治疗效果评价。结果:在感染初期,3个组IL-8、TNF-α及IFN-γ含量都升高,而IL-10有降低趋势。在感染后第7天,C组IL-8浓度显著低于A组(P<0.05);在第14—21天,B组极显著高于A组(P<0.01)。在第5天,C组IL-10显著高于A组(P<0.05),极显著低于B组(P<0.01);在第14天,C组极显著低于A组(P<0.01),显著高于B组(P<0.05);在第21天,C组显著低于A组(P<0.05),极显著高于B组(P<0.01)。在第14天,C组IFN-γ含量显著高于A组(P<0.05);在第21天,C组显著低于B组(P<0.05),但极显著高于A组(P<0.01)。在感染第14天,C组TNF-α显著低于B组(P<0.05),显著高于A组(P<0.05);在第21天,C组的TNF-α显著低于B组(P<0.05),但极显著高于A组(P<0.01)。组织病理学评分结果:A组平均分为9.4,B组平均分19.9,C组平均分为12.8,B组评分显著高于A、C组(P<0.05、P<0.05)。rBPI对MO感染的杂交羊有治疗作用,对细胞因子有调节作用。 展开更多
关键词 杀菌/通透性增加蛋白 杂交羊 绵羊肺炎支原体 细胞因子 病理评分
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