Baizhi, as a medicinal plant, has been demonstrated to be useful for the treatment of aches and pains in China. The physicochemical characterization of Baizhi particles is greatly influenced by ultrafine pulverization...Baizhi, as a medicinal plant, has been demonstrated to be useful for the treatment of aches and pains in China. The physicochemical characterization of Baizhi particles is greatly influenced by ultrafine pulverization. To study the physicochemical characterization of Baizhi, the raw plant material of Baizhi was ground to 6 μm particles by a high speed centrifugal sheering （HSCS） pulverizer. The micron particles were characterized by optical microscopy and scanning electron microscopy （SEM）. Imperatorin is one of the active ingredients of Baizhi, and its extraction yield is determined to evaluate the chemical characterization of Baizhi powder. Imperatorin was analyzed by high performance liquid chromatography （HPLC）. The results show that after ultrafine pulverization, the plant cell walls are broken into pieces and the extraction yield of imperatorin is increased by 11.93% compared with the normal particles.展开更多
目的:利用效应面法优选白芷的醇提取工艺参数。方法:采用HPLC方法,Wat er s公司的Sunf i r e C18色谱柱(150 mm×4.6mm,5μm);流动相为甲醇-水(70∶30),等度洗脱;流速0.9 m L/mi n;检测波长254nm;柱温30℃;分析时间为20分钟;进样量1...目的:利用效应面法优选白芷的醇提取工艺参数。方法:采用HPLC方法,Wat er s公司的Sunf i r e C18色谱柱(150 mm×4.6mm,5μm);流动相为甲醇-水(70∶30),等度洗脱;流速0.9 m L/mi n;检测波长254nm;柱温30℃;分析时间为20分钟;进样量10μL。结果:白芷中欧前胡素的最佳提取工艺为甲醇浓度83.17%,提取时间9.15小时,甲醇用量为147.32 m L;异欧前胡素的最佳提取工艺为甲醇浓度91.83%,提取时间9.15小时,甲醇用量为112.68 m L。结论:采用星点设计与效应面法相结合的方法对实验工艺优化更方便、更准确。展开更多
基金supported by the National High-Tech Research and Development Program of China (No.2009AA043202)the Special Project of State Administration of Traditional Chinese Medicine, China (No.200807054)
文摘Baizhi, as a medicinal plant, has been demonstrated to be useful for the treatment of aches and pains in China. The physicochemical characterization of Baizhi particles is greatly influenced by ultrafine pulverization. To study the physicochemical characterization of Baizhi, the raw plant material of Baizhi was ground to 6 μm particles by a high speed centrifugal sheering （HSCS） pulverizer. The micron particles were characterized by optical microscopy and scanning electron microscopy （SEM）. Imperatorin is one of the active ingredients of Baizhi, and its extraction yield is determined to evaluate the chemical characterization of Baizhi powder. Imperatorin was analyzed by high performance liquid chromatography （HPLC）. The results show that after ultrafine pulverization, the plant cell walls are broken into pieces and the extraction yield of imperatorin is increased by 11.93% compared with the normal particles.
文摘目的:利用效应面法优选白芷的醇提取工艺参数。方法:采用HPLC方法,Wat er s公司的Sunf i r e C18色谱柱(150 mm×4.6mm,5μm);流动相为甲醇-水(70∶30),等度洗脱;流速0.9 m L/mi n;检测波长254nm;柱温30℃;分析时间为20分钟;进样量10μL。结果:白芷中欧前胡素的最佳提取工艺为甲醇浓度83.17%,提取时间9.15小时,甲醇用量为147.32 m L;异欧前胡素的最佳提取工艺为甲醇浓度91.83%,提取时间9.15小时,甲醇用量为112.68 m L。结论:采用星点设计与效应面法相结合的方法对实验工艺优化更方便、更准确。
