The disease of banana Fusarium wilt currently threatens banana production areas all over the world.Rapid and large-area monitoring of Fusarium wilt disease is very important for the disease treatment and crop planting...The disease of banana Fusarium wilt currently threatens banana production areas all over the world.Rapid and large-area monitoring of Fusarium wilt disease is very important for the disease treatment and crop planting adjustments.The objective of this study was to evaluate the performance of supervised classification algorithms such as support vector machine(SVM),random forest(RF),and artificial neural network(ANN)algorithms to identify locations that were infested or not infested with Fusarium wilt.An unmanned aerial vehicle(UAV)equipped with a five-band multi-spectral sensor(blue,green,red,red-edge and near-infrared bands)was used to capture the multi-spectral imagery.A total of 139 ground sample-sites were surveyed to assess the occurrence of banana Fusarium wilt.The results showed that the SVM,RF,and ANN algorithms exhibited good performance for identifying and mapping banana Fusarium wilt disease in UAV-based multi-spectral imagery.The overall accuracies of the SVM,RF,and ANN were 91.4%,90.0%,and 91.1%,respectively for the pixel-based approach.The RF algorithm required significantly less training time than the SVM and ANN algorithms.The maps generated by the SVM,RF,and ANN algorithms showed the areas of occurrence of Fusarium wilt disease were in the range of 5.21-5.75 hm2,accounting for 36.3%-40.1%of the total planting area of bananas in the study area.The results also showed that the inclusion of the red-edge band resulted in an increase in the overall accuracy of 2.9%-3.0%.A simulation of the resolutions of satellite-based imagery(i.e.,0.5 m,1 m,2 m,and 5 m resolutions)showed that imagery with a spatial resolution higher than 2 m resulted in good identification accuracy of Fusarium wilt.The results of this study demonstrate that the RF classifier is well suited for the identification and mapping of banana Fusarium wilt disease from UAV-based remote sensing imagery.The results provide guidance for disease treatment and crop planting adjustments.展开更多
Banana(Musa spp.) is seriously threatened by the soil-borne fungus Fusarium oxysporum f. sp. cubense(Foc), also known as Panama disease.Attempts to control Fusarium wilt with fungicides damage soil health and have lim...Banana(Musa spp.) is seriously threatened by the soil-borne fungus Fusarium oxysporum f. sp. cubense(Foc), also known as Panama disease.Attempts to control Fusarium wilt with fungicides damage soil health and have limited efficiency due to pathogenic variability. Elucidating the mechanism of infection and molecular basis of host defense through banana genome sequencing, genome editing and proteomic profile analysis will help formulate strategies to develop resistant cultivars. This will include research to better understand the functions of Fusarium wilt-resistance proteins. Transgenic approaches and protoplast fusion could be employed as tools for transferring resistance genes from wild relatives to commercial banana varieties, and may serve as a new strategy in solving the problems faced by banana breeding programmes.Evaluation of banana germplasm for resistance to Fusarium wilt using in vitro mutation and selection, along with somaclonal variation and somatic hybridization, could improve banana breeding efficiency for resistance against Foc. Plant hormones could also play an important role in regulating plant growth and defense by mediating developmental processes and signaling networks involved in banana responses to Foc. A complementary approach for managing Fusarium wilt, such as exclusion, surveillance and biological control as important components of integrated disease management programs must be considered to prevent and contain contagion. This includes studies on banana plant-microbe interactions, embracing both plant growth promoting rhizobacteria(PGPR) to induce Foc resistance, and exploring Foc-derived elicitors for inducing defense-related enzymes in bananas. The role of Silicon and crop and livestock integration must also be included in the Fusarium control toolbox. The current review also gathers knowledge of the biotechnological approaches along with biological control of Fusarium wilt of banana that will provide researchers insights and criteria to develop future studies.展开更多
This study is aimed at assessing the ability of two endophytic bacteria originally isolated from healthy oil palm roots, Pseudomonas sp. （UPMP3） and Burkholderia sp. （UPMB3） to induce resistance in susceptible Ber...This study is aimed at assessing the ability of two endophytic bacteria originally isolated from healthy oil palm roots, Pseudomonas sp. （UPMP3） and Burkholderia sp. （UPMB3） to induce resistance in susceptible Berangan banana against Fusarium oxysporum race 4 （FocR4）. Increased accumulation of resistance-related enzymes such as peroxidase （PO）, phenylalanine ammonia lyase （PAL）, lignithioglycolic acid （LTGA）, and pathogenesis-related （PR） proteins （ehitinase and β-1,3-glucanase） has been observed in plantlets treated with endophytic bacteria UPMP3 and UPMB3 singly or as mixture under glasshouse conditions. Pre-inoculation of banana plantlets with UPMP3 showed a significant reduction in Fusarium wilt incidence 72 d after challenged inoculation with FocR4. UPMB3 was less effective in suppressing Fusarium wilt compared to UPMP3, whereas, the mixture of both endophytes showed an intermediate effect. Based on these results, it is concluded that UPMP3 could be a promising biological control agent that can trigger resistance against Fusarium wilt in susceptible Berangan banana.展开更多
Fusarium wilt of banana, which is caused by Fusarium oxysporum f. sp. cubense tropical race 4 （Foc TR4）, is a serious soil-borne fungal disease. Now, the epigenetic molecular pathogenic basis is elusive. In this stu...Fusarium wilt of banana, which is caused by Fusarium oxysporum f. sp. cubense tropical race 4 （Foc TR4）, is a serious soil-borne fungal disease. Now, the epigenetic molecular pathogenic basis is elusive. In this study, with methylation-sensitive amplification polymorphism （MSAP） technique, DNA methylation was compared between the leaves inoculated with Foc TR4 and the mock-inoculated leaves at different pathogenic stages. With 25 pairs of primers, 1 144 and 1 255 fragments were amplified from the infected and mock-inoculated leaves, respectively. DNA methylation was both changed and the average methylated CCGG sequences were 34.81 and 29.26% for the infected and the mock-inoculated leaves. And DNA hypermethylation and hypomethylation were induced by pathogen infection during all pathogenic stages. Further, 69 polymorphic fragments were sequenced and 29 of them showed sequence similarity to genes with known functions. And RT-PCR results of four genes indicated that their expression patterns were consistent with their methylation patterns. Our results suggest that DNA methylation plays important roles in pathogenic response to Foc TR4 for banana.展开更多
Effective control strategies are lacking for Fusarium wilt of banana crops worldwide. Here, the inhibitory efficacy of suspensions derived from Tilapia offal by anaerobic fermentation against Fusarium oxysporum f. sp....Effective control strategies are lacking for Fusarium wilt of banana crops worldwide. Here, the inhibitory efficacy of suspensions derived from Tilapia offal by anaerobic fermentation against Fusarium oxysporum f. sp. cubense tropical race 4 (Foc TR4) was evaluated. Two anaerobic fermentation methods were used: 1) natural fermentation of offal (NF) and 2) fermentation of offal supplemented with 5% lime (LF). The suspensions were applied in three treatments: Plate assay, pot system, and in the field. The inhibition rate and disease index were determined. The results showed that the inhibition was significantly greater for LF than for NF on plates. In pot system and in the field, the disease index was lower for the LF group than for the NF group and was significantly lower than that of the control. Therefore, suspensions derived from anaerobically fermented offal provide a new control method for Fusarium wilt in banana.展开更多
Fusarium wilt of banana, caused by Fusarium oxysporum f. sp. cubense tropic race 4(Foc TR4), is a typical vascular and soil-borne disease which has significantly threatened the sustainable development of banana indust...Fusarium wilt of banana, caused by Fusarium oxysporum f. sp. cubense tropic race 4(Foc TR4), is a typical vascular and soil-borne disease which has significantly threatened the sustainable development of banana industry. In order to reveal the infection process and pathogenesis of Foc TR4, the young mycelia(66.7 mg/ml) of wild-type strain of Foc TR4(WT-Foc TR4) cultured for 18-20 h were lysed with enzyme mixture for protoplast formation, which consisted of 25 mg/ml driselase, 0.4 mg/ml chitinase, 15 mg/ml lysing enzyme and 1.2 mol/L potassium chloride. The resulted protoplasts of 2×10~7 cells/ml were used to test the efficiency of transformation mediated by polyethylene glycol, and up to 9 transformants per microgram of DNA were obtained. AmCyan, RFP and YFP genes were stably transferred into the WT-Foc TR4, separately, using the protoplast transformation system. The gene FoOCH1 encoding α-1, 6-mannosyltransferase in the WT-Foc TR4 was knocked out using the split-marker recombination technology. The genetic transformation and gene knockout system in this pathogen lays a foundation for the study of functional genomics and plant-pathogen interactions.展开更多
基金This research was funded by the Hainan Provincial Key R&D Program of China(ZDYF2018073)National Natural Science Foundation of China(41571354)+2 种基金Hainan Provincial Major Science and Technology Program of China(ZDKJ2019006)Agricultural Science and Technology Innovation of Sanya,China(2016NK16)National Special Support Program for High-level Personnel Recruitment(Ten-thousand Talents Program)(Wenjiang Huang),Innovation Foundation of Director of Institute of Remote Sensing and Digital Earth,Chinese Academy of Sciences.We also gratefully acknowledge the National Meteorological Information Center of China,Guangxi Jiejiarun Technology Co.,Ltd.and Guangxi Jinsui Agriculture Group Co.,Ltd.for the experiments.
文摘The disease of banana Fusarium wilt currently threatens banana production areas all over the world.Rapid and large-area monitoring of Fusarium wilt disease is very important for the disease treatment and crop planting adjustments.The objective of this study was to evaluate the performance of supervised classification algorithms such as support vector machine(SVM),random forest(RF),and artificial neural network(ANN)algorithms to identify locations that were infested or not infested with Fusarium wilt.An unmanned aerial vehicle(UAV)equipped with a five-band multi-spectral sensor(blue,green,red,red-edge and near-infrared bands)was used to capture the multi-spectral imagery.A total of 139 ground sample-sites were surveyed to assess the occurrence of banana Fusarium wilt.The results showed that the SVM,RF,and ANN algorithms exhibited good performance for identifying and mapping banana Fusarium wilt disease in UAV-based multi-spectral imagery.The overall accuracies of the SVM,RF,and ANN were 91.4%,90.0%,and 91.1%,respectively for the pixel-based approach.The RF algorithm required significantly less training time than the SVM and ANN algorithms.The maps generated by the SVM,RF,and ANN algorithms showed the areas of occurrence of Fusarium wilt disease were in the range of 5.21-5.75 hm2,accounting for 36.3%-40.1%of the total planting area of bananas in the study area.The results also showed that the inclusion of the red-edge band resulted in an increase in the overall accuracy of 2.9%-3.0%.A simulation of the resolutions of satellite-based imagery(i.e.,0.5 m,1 m,2 m,and 5 m resolutions)showed that imagery with a spatial resolution higher than 2 m resulted in good identification accuracy of Fusarium wilt.The results of this study demonstrate that the RF classifier is well suited for the identification and mapping of banana Fusarium wilt disease from UAV-based remote sensing imagery.The results provide guidance for disease treatment and crop planting adjustments.
基金supported by the National Natural Science Foundation of China (NSFC31560505)Science and Technology Department of Yunnan Provincial Government (2015HA033 and 2015HA034)
文摘Banana(Musa spp.) is seriously threatened by the soil-borne fungus Fusarium oxysporum f. sp. cubense(Foc), also known as Panama disease.Attempts to control Fusarium wilt with fungicides damage soil health and have limited efficiency due to pathogenic variability. Elucidating the mechanism of infection and molecular basis of host defense through banana genome sequencing, genome editing and proteomic profile analysis will help formulate strategies to develop resistant cultivars. This will include research to better understand the functions of Fusarium wilt-resistance proteins. Transgenic approaches and protoplast fusion could be employed as tools for transferring resistance genes from wild relatives to commercial banana varieties, and may serve as a new strategy in solving the problems faced by banana breeding programmes.Evaluation of banana germplasm for resistance to Fusarium wilt using in vitro mutation and selection, along with somaclonal variation and somatic hybridization, could improve banana breeding efficiency for resistance against Foc. Plant hormones could also play an important role in regulating plant growth and defense by mediating developmental processes and signaling networks involved in banana responses to Foc. A complementary approach for managing Fusarium wilt, such as exclusion, surveillance and biological control as important components of integrated disease management programs must be considered to prevent and contain contagion. This includes studies on banana plant-microbe interactions, embracing both plant growth promoting rhizobacteria(PGPR) to induce Foc resistance, and exploring Foc-derived elicitors for inducing defense-related enzymes in bananas. The role of Silicon and crop and livestock integration must also be included in the Fusarium control toolbox. The current review also gathers knowledge of the biotechnological approaches along with biological control of Fusarium wilt of banana that will provide researchers insights and criteria to develop future studies.
基金the Research University Grants(RUGS 91009),Malaysia,for financing this research
文摘This study is aimed at assessing the ability of two endophytic bacteria originally isolated from healthy oil palm roots, Pseudomonas sp. （UPMP3） and Burkholderia sp. （UPMB3） to induce resistance in susceptible Berangan banana against Fusarium oxysporum race 4 （FocR4）. Increased accumulation of resistance-related enzymes such as peroxidase （PO）, phenylalanine ammonia lyase （PAL）, lignithioglycolic acid （LTGA）, and pathogenesis-related （PR） proteins （ehitinase and β-1,3-glucanase） has been observed in plantlets treated with endophytic bacteria UPMP3 and UPMB3 singly or as mixture under glasshouse conditions. Pre-inoculation of banana plantlets with UPMP3 showed a significant reduction in Fusarium wilt incidence 72 d after challenged inoculation with FocR4. UPMB3 was less effective in suppressing Fusarium wilt compared to UPMP3, whereas, the mixture of both endophytes showed an intermediate effect. Based on these results, it is concluded that UPMP3 could be a promising biological control agent that can trigger resistance against Fusarium wilt in susceptible Berangan banana.
基金supported by the National Natural Science Foundation of China (30860149 and 31360364)the Joint Support Program from Tropical Crop Breeding Engineering Center of Ministry of Education of China+1 种基金the Crop Science National Key Disciplines of China (lhxm-2012-2)the Key Scientific Research Program from Hainan Province,China (ZDZX2013023)
文摘Fusarium wilt of banana, which is caused by Fusarium oxysporum f. sp. cubense tropical race 4 （Foc TR4）, is a serious soil-borne fungal disease. Now, the epigenetic molecular pathogenic basis is elusive. In this study, with methylation-sensitive amplification polymorphism （MSAP） technique, DNA methylation was compared between the leaves inoculated with Foc TR4 and the mock-inoculated leaves at different pathogenic stages. With 25 pairs of primers, 1 144 and 1 255 fragments were amplified from the infected and mock-inoculated leaves, respectively. DNA methylation was both changed and the average methylated CCGG sequences were 34.81 and 29.26% for the infected and the mock-inoculated leaves. And DNA hypermethylation and hypomethylation were induced by pathogen infection during all pathogenic stages. Further, 69 polymorphic fragments were sequenced and 29 of them showed sequence similarity to genes with known functions. And RT-PCR results of four genes indicated that their expression patterns were consistent with their methylation patterns. Our results suggest that DNA methylation plays important roles in pathogenic response to Foc TR4 for banana.
文摘Effective control strategies are lacking for Fusarium wilt of banana crops worldwide. Here, the inhibitory efficacy of suspensions derived from Tilapia offal by anaerobic fermentation against Fusarium oxysporum f. sp. cubense tropical race 4 (Foc TR4) was evaluated. Two anaerobic fermentation methods were used: 1) natural fermentation of offal (NF) and 2) fermentation of offal supplemented with 5% lime (LF). The suspensions were applied in three treatments: Plate assay, pot system, and in the field. The inhibition rate and disease index were determined. The results showed that the inhibition was significantly greater for LF than for NF on plates. In pot system and in the field, the disease index was lower for the LF group than for the NF group and was significantly lower than that of the control. Therefore, suspensions derived from anaerobically fermented offal provide a new control method for Fusarium wilt in banana.
基金云南省万人计划“云岭学者”项目(YNWR-YLXZ-2018-018)国际原子能机构(International Atomic Energy Agency)项目“An IntegrativeApproach to Enhance Disease Resistance Against Fusarium Wilt(Foc TR4)in Banana–Phase II”(D23033-26673)。
基金Supported by Yunnan Science and Technology Innovation Talent Program(2015HA034)National Natural Science Foundation of China(NSFC31560505)
文摘Fusarium wilt of banana, caused by Fusarium oxysporum f. sp. cubense tropic race 4(Foc TR4), is a typical vascular and soil-borne disease which has significantly threatened the sustainable development of banana industry. In order to reveal the infection process and pathogenesis of Foc TR4, the young mycelia(66.7 mg/ml) of wild-type strain of Foc TR4(WT-Foc TR4) cultured for 18-20 h were lysed with enzyme mixture for protoplast formation, which consisted of 25 mg/ml driselase, 0.4 mg/ml chitinase, 15 mg/ml lysing enzyme and 1.2 mol/L potassium chloride. The resulted protoplasts of 2×10~7 cells/ml were used to test the efficiency of transformation mediated by polyethylene glycol, and up to 9 transformants per microgram of DNA were obtained. AmCyan, RFP and YFP genes were stably transferred into the WT-Foc TR4, separately, using the protoplast transformation system. The gene FoOCH1 encoding α-1, 6-mannosyltransferase in the WT-Foc TR4 was knocked out using the split-marker recombination technology. The genetic transformation and gene knockout system in this pathogen lays a foundation for the study of functional genomics and plant-pathogen interactions.
