关于Barcode自助借还机在高校图书馆应用满意度的实证研究——以江苏海洋大学图书馆为例

随着智慧图书馆概念的提出,Barcode(条形码)识别模式自助借还机作为一项新型的、日趋成熟的服务方式正广泛应用于图书馆。基于此,以Barcode自助借还机的使用情况为调研对象,了解读者和管理员使用后的满意度,以便发现自助借还机在使用过程中的各种利弊,并提出解决问题的有效途径。

COI序列:影响动物分类学与生态学的DNA barcode

DNA barcode是一段特殊的、可用于物种鉴定的DNA序列。目前在动物中最常用的DNA barcode是细胞色素C氧化酶1号基因(COI)的部分序列。随着标准数据库的建立,基于COI基因的DNA barcode在动物分类学和生态学中得到了广泛应用。但是,采用COI基因作为DNA barcode所隐含的涉及线粒体的进化历史、遗传特性和物种成种时间的默认前提,并非完全成立,由此引发了许多问题。本文阐述了基于COI基因的DNA barcode对分类学和生态学的影响,目前存在的问题,以及可能的研究方向。

Three newly recorded species of Acerentomata(Hexapoda:Protura) from China,with analysis of DNA barcodes

Three newly recorded species in the order Acerentomata in Protura from China are described:Filientomon duodecimsetosum Nakamura,2004,Verrucoentomon anatoli Shrubovych & Bernard,2012 and Verrucoentomon louisanne Shrubovych & Bernard,2012.The important morphological characters of Chinese specimens are described in detail.An updated key to Chinese Verrucoentomon species is provided.In addition,their DNA barcodes are sequenced and analyzed.

Barcode技术在ERP框架下生产过程管理中的应用研究

针对离散生产企业车间管理的黑箱作业,提出Barcode技术在企业资源计划(ERP)下生产过程管理中的应用框架,总结出Barcode技术在ERP下生产过程管理应用架构的三个主要技术,并介绍一种先进物料清单(BOM)设计和条码组合设计的思路。最后结合实施的多个案例分析此模式在离散制造业的应用。

Evaluating the Discriminatory Power of DNA Barcodes in Panicoideae, Poaceae

DNA barcoding is a powerful technique for species identification with little morphological knowledge, by using short sections of DNA from a specific region of the genome. Two core barcode markers, rbcL and matK, and a supplementary nuclear ribosomal internal transcribed spacer region were used to examine the effectiveness of the markers for Poaceae barcoding using 133 individuals of 36 taxa across 23 genera of Korean Panicoideae. We also aimed to establish a DNA barcode database for the major weeds of Korean Panicoideae. All three markers revealed a good level of amplification and sequencing success. As a single DNA marker, the ITS region achieved the highest species resolution, followed by matK. Resolving power was increased when nrlTS was incorporated into the core barcode markers. The best resolving power was obtained with a combination of matK ＋ ITS with 89.7%, followed by rbcL ＋ matK ＋ ITS with 89.3%. Thus, rbcL may be not necessary as a DNA barcode for Panicoideae species identification, when considering cost and effectiveness. Instead, a combination of matK ＋ ITS is proposed as the most suitable DNA barcode for the species identification of Panicoideae, Poaceae. We conclude that DNA barcoding using a combination of matK ＋ ITS could be one of powerful techniques for the identification of Poaceae species, The barcode sequences were deposited to the National Center for Biotechnology Information （NCBI） database for public use.

Barcode技术:增加输血安全性的作用

背景 错误的血液成分输入是最常见的输血相关的严重事故,这些频繁发生的错误原因包括病人的错误辨识。设计和方法 本研究评估Barcode病人辨识系统,包括手提电脑对采集血样的兼容性试验和血液管理的作用,和应用Barcode前后的作用。结果 基线检查显示尤其是病人辨识的实用性差。

基于barcode4j的独立学院图书馆资产管理平台——以华南师范大学增城学院为例

文章阐述了Barcode4j的原理和开发方法,对平台的设计与开发进行了分析,提出了在校园网上开发图书馆资产网络管理平台的思路。

Application of DNA Barcode Technology to the Identification of Exotic Mosquitoes at Ningbo Port

[Objective] The study aimed to solve the problem of morphological identi- fication difficulties and propose solutions for the identification of foreign mosquitoes that are difficult to identify. [Method] Based on the sequencing, alignment and anal- ysis of COl gene fragment, DNA barcode technology was used to identify 7 exotic mosquitoes, and the phylogenetic analysis was performed using MEGA6.0 and DNASTAR software. Then the morphological characteristics of the mosquitoes were reviewed. [Result[ These mosquitoes were Armigeres subalbatus, Culex gefidus, Anopheles gambiae, and Culiseta incidens. [Conclusion] DNA barcode technology is a useful supplement to the morphological classification method of mosquitoes.

An Improved Barcoded Oligonucleotide Primers-based Next-generation Sequencing Approach for Direct Identification of Viral Pathogens in Clinical Specimens

Objective To provide a feasible and cost-effective next-generation sequencing （NGS） method for accurate identification of viral pathogens in clinical specimens, because enormous limitations impede the clinical use of common NGS, such as high cost, complicated procedures, tremendous data analysis, and high background noise in clinical samples. Methods Viruses from cell culture materials or clinical specimens were identified following an improved NGS procedure： reduction of background noise by sample preprocessing, viral enrichment by barcoded oligonucleotide （random hexamer or non-ribosomal hexanucleotide） primer-based amplification, fragmentation-free library construction and sequencing of one-tube mixtures, as well as rapid data analysis using an in-house pipeline. Results NGS data demonstrated that both barcoded primer sets were useful to simultaneously capture multiple viral pathogens in cell culture materials or clinical specimens and verified that hexanucleotide primers captured as many viral sequences as hexamers did. Moreover, direct testing of clinical specimens using this improved hexanucleotide primer-based NGS approach provided further detailed genotypes of enteroviruses causing hand, foot, and mouth disease （HFMD） and identified other potential viruses or differentiated misdiagnosis events. Conclusion The improved barcoded oligonucleotide primer-based NGS approach is simplified, time saving, cost effective, and appropriate for direct identification of viral pathogens in clinical practice.

Barcode与RFID识别模式的自助借还系统比较分析

在论述Barcode与RFID识别模式图书馆自助借还系统原理的基础上,从成本、使用、管理和安全4方面分析两个模式现阶段应用于图书馆自助系统的优劣,并对上述两种识别模式的未来发展趋势进行了分析与展望。

Application of Evolutionary Encryption 2D Barcode Generation Technology in Agricultural Product Quality and Safety Traceability System

Two-dimensional(2D) barcode technology is an electronic tagging technology based on combination of computer and optical technology. It is an important way of information collection and input. 2D barcode technology has been widely used in various fields of logistics,production automation,and e-commerce,but it also has brought about a series of safety problems. Based on evolutionary encryption technology,this paper improved algorithm of traditional 2D barcode generation,to improve forgery- proof performance of 2D barcode. This algorithm is applied to agricultural products quality and safety traceability system and the results show that it is effective.

A WATERMARKING ALGORITHM BASED ON PERMUTATION AND 2-D BARCODE

This letter presents a method for digital image watermarking for copyright protection. This technique produces a watermarked image that closely retains the quality of the original host image while concurrently surviving various image processing operations such as lowpass/highpass filtering, lossy JPEG compression, and cropping. This image watermarking algorithm takes full advantage of permutation and 2-D barcode (PDF417). The actual watermark embedding in spatial domain is followed using permutated image for improving the resistance to image cropping. Much higher watermark robustness is obtainable via a simple forward error correction technique, which is the main feature of PDF417 codes. Additional features of this technique include the easy determination of the existence of the watermark and that the watermark verification procedure does not need the original host image. The experimental results demonstrate its effectiveness.

DNA Barcode Screening and Preliminary Study on Phylogeny of Sea Snake Based on Genes COI and cytb

[Objectives]The most common gene fragment used in animal DNA barcode technology is COI,but it is not necessarily suitable for all species.This study was conducted to screen genes suitable for the DNA barcode of sea snakes.[Methods]All COI and cytb gene sequences on GenBank were searched and downloaded.After the comparison with Mega software,clustering trees of MrBayes system were established.[Results]Interspecies distances were greater than intraspecies distances for the two genes.The topological structures of their molecular hierarchical clustering trees were clear,and the support rates were high.[Conclusions]Therefore,it is concluded that not the DNA barcode of each species must be gene COI.Cytb is more suitable in terms of the mitochondrial gene of sea snakes.

Crane flies(Diptera: Tipuloidea: Tipulidae) from Dayaoshan National Nature Reserve, China and analysis of DNA barcodes, with description of one new species in the genus Indotipula

This paper reports six species of crane flies from Dayaoshan National Nature Reserve, Guangxi Zhuang Autonomous Region, China, including one new species, Indotipula jinxiuensis sp. nov. The males of Pselliophora guangxiensis Yang Yang, 1988 and Holorusia basiflava Yang Yang, 1993 and female of Pselliophora xanthopimplina Enderlein, 1921 are redescribed and illustrated with new morphological characters. The females of P. guangxiensis and H. basiflava are described and illustrated for the first time. A key for separating known species of Indotipula Edwards, 1931 from China is provided. DNA barcodes of all species in this study are provided and analyzed.

DNA Barcodes in Fig Cultivars (Ficus carica L.) Using ITS Regions of Ribosomal DNA, the psbA-trnH Spacer and the matK Coding Sequence

Molecular markers provide a useful method for genotype characterization and allow a high precision determination of the genetic relationship between cultivars and varieties. A system based on DNA sequences—which is known as DNA barcoding—will choose one or several standard loci which can be sequenced and compared to differentiate between species. In this research, the ITS, matK, and trnH-psbA sequences were evaluated for the molecular identification of seven F. carica genotypes, generating complete sequences for the first two loci, but unable to produce bidirectional sequences by using the trnH-psbA sequence. The ITS sequence presented the highest variation rates, while the phylogeny constructed with the matK sequence obtained the highest percentage of solved monophyletic groups. Through Pearson’s correlation analysis, it was possible to determine the existence of a significant correlation between the ITS region and psbA-trnH, and the matK and psbA-trnH sequences, but not between ITS and matK. The phylogenies constructed with the ITS + matK barcodes and ITS + matK + psbA-trnH presented the highest percentage for resolution. However, considering the cost efficiency and the facilitated recovery by using PCR, the matK + ITS combination is recommended.

Construction of a New Molecular Barcode for Discriminating Plants and Animals with a Close Genetic Relationship

DNA barcodes have been proposed as a shortcut to provide species identification and as a way to accelerate the discovery of new species. A number of candidate gene regions have been suggested as possible barcodes for animals and plants, but for the identification of recently diverged species and/or varieties with only a few genetic differences it has been reported to be problematic in some cases. This study selected widely cultivated cruciferous vegetables as the primary samples, after failure of discrimination of each species using current DNA barcodes, we performed the fluorescent amplified fragment length polymorphism （F-AFLP） and successfully discriminated each species, subspecies, variety and their cultivar in 74 samples. Then the non-qualitative results obtained from F-AFLP were transformed into two-dimensional barcodes image file of each cultivar via the PDF417 software. This method was also successfully applied to the discrimination of 17 Chinese indigenous pig breeds. The barcode we constructed which greatly reduces the information storage space is genotypes-specific, and can be conveniently decoded into the original data and thereby be conveniently shared and referred to. We believe that it is possible to construct a new data sharing molecular barcode system that could discriminate the subspecies, varieties, cultivars and even individuals with close genetic relationships.

Emergence of Plastidial Intergenic Spacers as Suitable DNA Barcodes for Arid Medicinal Plant <i>Rhazya stricta</i>

The desert plant Rhazya stricta has anticancer and antimicrobial properties, and is widely used in indigenous medicines of Saudi Arabia. However, the therapeutic benefits rely on an accurate identification of this species. The authenticity of R. stricta and other medicinal plants and herbs procured from local markets can be questionable due to a lack of clear phenotypic traits. DNA barcoding is an emerging technology for rapid and accurate species identification. In this study, six candidate chloroplastid barcodes were investigated for the authentication of R. stricta. We compared the DNA sequences from fifty locally collected and five market samples of R. stricta with database sequences of R. stricta and seven closely related species. We found that the coding regions matK, rbcL, rpoB, and rpoC1 were highly similar among the taxa. By contrast, the intergenic spacers psbK-psbI and atpF-atpH were variable loci distinct for the medicinal plant R. stricta. psbK-psbI clearly discriminated R. stricta samples as an efficient single locus marker, whereas a two-locus marker combination comprising psbK-psbI + atpF-atpH was also promising according to results from the Basic Local Alignment Search Tool and a maximum likelihood gene tree generated using PHyML. Two-dimensional DNA barcodes (i.e., QR codes) for the psbK-psbI and psbK-psbI + atpF-atpH regions were created for the validation of fresh or dried R. stricta samples.

Digital Barcode Development for Single Nuclotide Polymorphism (SNP) Identification of Suzhong Swine Individuals

Suzhong swine is a hybrid breed derived from Taihu sows and Landraee boars. To identify Suzhong swine individuals and trace the source of pork products, single nucleotide polymorphisms （SNPs） identification of Suzhong swine individuals was studied. A total of 29 pairs of primers were designed and sev- en pairs of primers were used for identification of Suzhong swine individuals. The products amplified by seven pairs of primers could be directly sequenced, with clean sequencing map background and no ambiguity in sequence read. Totally 52 SNPs loci were amplified by seven pairs of primers, and 41 SNPs loci were reserved for identification of Suzhong swine individuals through correlation analysis and heterezygosity filtration （ H ≥0.1 ）. Meantime, the digital barcodes for SNP identification of 96 individuals of Suzhong swine derived from seven boars and 12 sows were developed, which well distinguished 96 individuals of Suzhong swine. Theoretically, 41SNPs amplified by seven pairs of primers could be used for identification of 5.0 × 10^6 pig individuals. Therefore, digital barcode devel- opment method for SNP identification of Suzbong swine individuals can be used for individual identification of Suzhong swine in scale pig farm and meat product traceability.

Earthquake Barcode from a Single-Degree-of-Freedom System

Earthquake is a violent and irregular ground motion that can severely damage structures. In this paper we subject a single-degree-of-freedom system, consisting of spring and damper, to an earthquake excitation, and meanwhile investigate the response behavior from a novel theory about the dynamical system, by viewing the time-varying signum function of It can reflect the characteristic property of each earthquake through and the second component of f, where is a time-sampling record of the acceleration of a ground motion. The barcode is formed by plotting with respect to time. We analyze the complex jumping behavior in a barcode and an essential property of a high percentage occupation of the first set of dis-connectivity in the barcode from four strong earthquake records: 1940 El Centro earthquake, 1989 Loma earthquake, and two records of 1999 Chi-Chi earthquake. Through the comparisons of four earthquakes, we can observe that strong earthquake leads to large percentage of the first set of dis-connectivity.

A High-Density Ternary Barcode Detection System with a Dual-Bias Differential Method

A dual-bias differential method is presented for increasing the detection range of a ternary barcode detection system. The system is provided with a second differential delay circuit with bias control to process optimally gray signals by lowering their averaged level using a clamping circuit. This is added to the primary conventional differential delay circuit without bias control and a comparator to process optimally black signals based on the envelope-differential fixed-period delay (EDFPD) detection technique. This method enables the system to detect over a longer range at high speeds while being capable of handling a large amount of information. The estimate results of gray and white code widths against the clamp bias made through the dynamic operation simulation of a differential circuit using SPICE were nearly consistent with the experimental results. Thereby we can conclude that the dynamic simulation is effective for estimation of an optimum clamp bias voltage. It was confirmed that the detection range of the system with a clamp bias voltage of ?0.4 V for a minimum bar width W = 0.25 mm was 1.4 times that of the conventional EDFPD detection technique. In addition, the system operated at a maximum scanning speed of 7.7 times that of conventional CCD cameras under the practical detection range. The system with clamp bias control is expected to enable the real-time identification of goods on production lines and in automated warehouses.