The application of molecular biology technology in the identification and quality control of Mongolian medicine is increasing gradually,and it provides a new method for identifying fake and inferior products and confu...The application of molecular biology technology in the identification and quality control of Mongolian medicine is increasing gradually,and it provides a new method for identifying fake and inferior products and confused products of Mongolian medicine.In this paper,the application and prospect of molecular biology technology(such as DNA barcoding and PCR molecular identification technique)in the identification of crude Mongolian medicine were reviewed.展开更多
The genus Pyrrosia belongs to the family Polypodiaceae and are medium-sized epiphytic ferns,where the dried leaves of Pyrrosia lingua,Pyrrosia sheareri,Pyrrosia lanceolata,and Pyrrosia calvata are commonly used in med...The genus Pyrrosia belongs to the family Polypodiaceae and are medium-sized epiphytic ferns,where the dried leaves of Pyrrosia lingua,Pyrrosia sheareri,Pyrrosia lanceolata,and Pyrrosia calvata are commonly used in medicinal practice.In this study,the authenticity of the collected medicinal plant samples of Shiwei was identified with the help of DNA barcoding technology using the internal transcribed spacer 2(ITS2)as the identifying sequence.The experimental samples were analyzed using the basic local alignment search tool(BLAST)and the authenticity of the samples was further verified with the results of similarity comparison.The results proved that the sequences of the experimentally collected samples of Pyrrosia lingua,Pyrrosia sheareri,Pyrrosia lanceolata,and Pyrrosia calvata had a similarity of more than 97%when compared with the corresponding sequences that were uploaded on the Internet.展开更多
Objective:To identify Ejiao and its adulterants at the DNA level by using DNA molecula marker.Ejiao(Asini Corii Colla)is a commonly used medicinal material.However,its adulteration is a serious concern.Due to the morp...Objective:To identify Ejiao and its adulterants at the DNA level by using DNA molecula marker.Ejiao(Asini Corii Colla)is a commonly used medicinal material.However,its adulteration is a serious concern.Due to the morphological characteristics of Asini Corii Colla and its adulterants,traditional identification techniques often complex and professional,which is not conducive to the circulation management and safety of the medicinal materials.To improve the distinction between Asini Corii Colla and its adulterants accurately,this study identified and its adulterant samples based on the CytB sequence.Sequence characteristics,Basic Local Alignment Search Tool(BLAST)application,genetic distance,construction of phylogenetic tree showed the CytB sequence to accurately identify Asini Corii Colla from its adulterants.Furthermore,in this study,we designed a specific primer,based on the CytB sequence,and established a PCR detection system for rapid,sensitive,and specific identification of Asini Corii Colla.Compared to DNA barcoding technology,this method has shorter detection time,stronger specificity,and higher sensitivity,which lays the foundation for the rapid identification of Asini Corii Colla.展开更多
基金Supported by the"First-class Discipline"Project of Mongolian Medicine in 2021(myxylxk202122)Collaborative Innovation Project of Inner Mongolia Autonomous Region(MYYXT202005)+1 种基金Scientific Research Project for Teachers of"First-class Discipline"of Mongolian Pharmacy in 2020(myxylxkky2020-04)Million Science and Technology Project of Inner Mongolia Medical University(YKD2018KJBW029).
文摘The application of molecular biology technology in the identification and quality control of Mongolian medicine is increasing gradually,and it provides a new method for identifying fake and inferior products and confused products of Mongolian medicine.In this paper,the application and prospect of molecular biology technology(such as DNA barcoding and PCR molecular identification technique)in the identification of crude Mongolian medicine were reviewed.
文摘The genus Pyrrosia belongs to the family Polypodiaceae and are medium-sized epiphytic ferns,where the dried leaves of Pyrrosia lingua,Pyrrosia sheareri,Pyrrosia lanceolata,and Pyrrosia calvata are commonly used in medicinal practice.In this study,the authenticity of the collected medicinal plant samples of Shiwei was identified with the help of DNA barcoding technology using the internal transcribed spacer 2(ITS2)as the identifying sequence.The experimental samples were analyzed using the basic local alignment search tool(BLAST)and the authenticity of the samples was further verified with the results of similarity comparison.The results proved that the sequences of the experimentally collected samples of Pyrrosia lingua,Pyrrosia sheareri,Pyrrosia lanceolata,and Pyrrosia calvata had a similarity of more than 97%when compared with the corresponding sequences that were uploaded on the Internet.
基金supported by Scientific Research Fund Projects of Hubei Food and Drug Administration in 2016-2018 under Grant No.20160212.
文摘Objective:To identify Ejiao and its adulterants at the DNA level by using DNA molecula marker.Ejiao(Asini Corii Colla)is a commonly used medicinal material.However,its adulteration is a serious concern.Due to the morphological characteristics of Asini Corii Colla and its adulterants,traditional identification techniques often complex and professional,which is not conducive to the circulation management and safety of the medicinal materials.To improve the distinction between Asini Corii Colla and its adulterants accurately,this study identified and its adulterant samples based on the CytB sequence.Sequence characteristics,Basic Local Alignment Search Tool(BLAST)application,genetic distance,construction of phylogenetic tree showed the CytB sequence to accurately identify Asini Corii Colla from its adulterants.Furthermore,in this study,we designed a specific primer,based on the CytB sequence,and established a PCR detection system for rapid,sensitive,and specific identification of Asini Corii Colla.Compared to DNA barcoding technology,this method has shorter detection time,stronger specificity,and higher sensitivity,which lays the foundation for the rapid identification of Asini Corii Colla.
