In order to study the effects of ethyl pyruvate on cardiomyocyte apoptosis following ischemia/reperfusion （I/R） in vitro and the expression of Bcl-2 and Bax proteins, isolated rat hearts were perfused in a Langendor...In order to study the effects of ethyl pyruvate on cardiomyocyte apoptosis following ischemia/reperfusion （I/R） in vitro and the expression of Bcl-2 and Bax proteins, isolated rat hearts were perfused in a Langendorff model. Twenty-four rats were randomly divided into 3 groups （n=8 in each group）： control group was perfused for 120 min. In the I/R group, after 30 min stabilization the injury was induced by 30 min global ischemia followed by 60 min reperfusion. Ethyl pyruvate （EP） group was set up with the same protocol as I/R group except that it was supplied with 2 mmol/L EP 15 rain before ischemia and throughout reperfusion. Myocardial malonaldehyde （MDA） content was measured. Myocardial apoptotic index （AI） was tested by terminal deoxynucleotidyl transferase mediated dUTP nick end labeling （TUNEL） method. The expression of anti-apoptotic protein Bcl-2 and pro-apoptotic protein Bax in cardiac myocytes was detected by immunohistochemistry. As compared with control group, the content of MDA, myocardial AI and the expression of Bcl-2, Bax proteins were increased significantly in I/R group, but the content of MDA, myocardial AI and the expression of Bax protein were decreased obviously and the expression of Bcl-2 protein was up-regulated in EP group （P〈0.05）. These results demonstrate that EP could inhibit apoptosis of cardiac myocytes possibly via alleviating oxidative stress, up-regulating Bcl-2 and down-regulating Bax proteins.展开更多
Tanshinone lla is an effective monomer component of Danshen, which is a traditional Chinese medicine for activating blood circulation to dissipate blood stasis. Tanshinone Ila can effectively improve brain tissue isch...Tanshinone lla is an effective monomer component of Danshen, which is a traditional Chinese medicine for activating blood circulation to dissipate blood stasis. Tanshinone Ila can effectively improve brain tissue ischemia/hypoxia injury. The present study established a rat model of spinal cord ischemia/reperfusion injury and intraperitoneally injected Tanshinone lla, 0.5 hour prior to model establishment. Results showed that Tanshinone Ila promoted heat shock protein 70 and Bcl-2 protein expression, but inhibited Bax protein expression in the injured spinal cord after ischemia/reperfusion injury. Furthermore, Nissl staining indicated a reduction in nerve cell apoptosis and fewer pathological lesions in the presence of Tanshinone Ila, compared with positive control Danshen injection.展开更多
目的观察昆母汤醇提液(KMT)对人类风湿关节炎成纤维样滑膜细胞(RA-FLS)B淋巴细胞瘤-2基因(bcl-2)、bcl-2相关X蛋白(bax)m RNA及蛋白的影响,探讨昆母汤治疗RA的部分作用机制。方法滑膜组织取自行关节置换术或关节镜检查术的8例活动性类...目的观察昆母汤醇提液(KMT)对人类风湿关节炎成纤维样滑膜细胞(RA-FLS)B淋巴细胞瘤-2基因(bcl-2)、bcl-2相关X蛋白(bax)m RNA及蛋白的影响,探讨昆母汤治疗RA的部分作用机制。方法滑膜组织取自行关节置换术或关节镜检查术的8例活动性类风湿关节炎(RA)患者,分离出FLS并进行原代培养,用逆转录-聚合酶链反应(RT-PCR)观察bax、bcl-2 m RNA表达的变化,采用酶联免疫吸附测定(ELISA)法检测bax、bcl-2蛋白含量的变化。结果与肿瘤坏死因子-α(TNF-α)组比较,KMT高剂量组可以明显促进bax m RNA表达,明显抑制bcl-2 m RNA表达,且bax m RNA/bcl-2 m RNA比值明显提高(P<0.05,P<0.01);KMT中、高剂量组可以提高bax蛋白含量,各剂量组均可降低bcl-2蛋白含量,bax/bcl-2的蛋白比值明显提高(P<0.05,P<0.01),且有一定的剂量依赖性。结论昆母汤可能通过抑制bcl-2的表达,同时促进bax的表达,调节bax/bcl-2比值来发挥对RA-FLS的促凋亡作用,具有治疗RA的潜在价值。展开更多
文摘In order to study the effects of ethyl pyruvate on cardiomyocyte apoptosis following ischemia/reperfusion （I/R） in vitro and the expression of Bcl-2 and Bax proteins, isolated rat hearts were perfused in a Langendorff model. Twenty-four rats were randomly divided into 3 groups （n=8 in each group）： control group was perfused for 120 min. In the I/R group, after 30 min stabilization the injury was induced by 30 min global ischemia followed by 60 min reperfusion. Ethyl pyruvate （EP） group was set up with the same protocol as I/R group except that it was supplied with 2 mmol/L EP 15 rain before ischemia and throughout reperfusion. Myocardial malonaldehyde （MDA） content was measured. Myocardial apoptotic index （AI） was tested by terminal deoxynucleotidyl transferase mediated dUTP nick end labeling （TUNEL） method. The expression of anti-apoptotic protein Bcl-2 and pro-apoptotic protein Bax in cardiac myocytes was detected by immunohistochemistry. As compared with control group, the content of MDA, myocardial AI and the expression of Bcl-2, Bax proteins were increased significantly in I/R group, but the content of MDA, myocardial AI and the expression of Bax protein were decreased obviously and the expression of Bcl-2 protein was up-regulated in EP group （P〈0.05）. These results demonstrate that EP could inhibit apoptosis of cardiac myocytes possibly via alleviating oxidative stress, up-regulating Bcl-2 and down-regulating Bax proteins.
基金supported by the National Natural Science Foundation of China,No.30973765New Century Excellent Talents Program,No. NECT-09-0013the Foundationfor Doctors,Ministry of Education,No.20113519110001
文摘Tanshinone lla is an effective monomer component of Danshen, which is a traditional Chinese medicine for activating blood circulation to dissipate blood stasis. Tanshinone Ila can effectively improve brain tissue ischemia/hypoxia injury. The present study established a rat model of spinal cord ischemia/reperfusion injury and intraperitoneally injected Tanshinone lla, 0.5 hour prior to model establishment. Results showed that Tanshinone Ila promoted heat shock protein 70 and Bcl-2 protein expression, but inhibited Bax protein expression in the injured spinal cord after ischemia/reperfusion injury. Furthermore, Nissl staining indicated a reduction in nerve cell apoptosis and fewer pathological lesions in the presence of Tanshinone Ila, compared with positive control Danshen injection.
文摘目的观察昆母汤醇提液(KMT)对人类风湿关节炎成纤维样滑膜细胞(RA-FLS)B淋巴细胞瘤-2基因(bcl-2)、bcl-2相关X蛋白(bax)m RNA及蛋白的影响,探讨昆母汤治疗RA的部分作用机制。方法滑膜组织取自行关节置换术或关节镜检查术的8例活动性类风湿关节炎(RA)患者,分离出FLS并进行原代培养,用逆转录-聚合酶链反应(RT-PCR)观察bax、bcl-2 m RNA表达的变化,采用酶联免疫吸附测定(ELISA)法检测bax、bcl-2蛋白含量的变化。结果与肿瘤坏死因子-α(TNF-α)组比较,KMT高剂量组可以明显促进bax m RNA表达,明显抑制bcl-2 m RNA表达,且bax m RNA/bcl-2 m RNA比值明显提高(P<0.05,P<0.01);KMT中、高剂量组可以提高bax蛋白含量,各剂量组均可降低bcl-2蛋白含量,bax/bcl-2的蛋白比值明显提高(P<0.05,P<0.01),且有一定的剂量依赖性。结论昆母汤可能通过抑制bcl-2的表达,同时促进bax的表达,调节bax/bcl-2比值来发挥对RA-FLS的促凋亡作用,具有治疗RA的潜在价值。
