Objective\ To understand the transcription of BamHI L DNA fragment from genome of strong virulent GA strain of Marek′s disease herpesvirus (MDV) in lymphoblastoid tumor tissue induced by oncogenic strain Beijing 1 ...Objective\ To understand the transcription of BamHI L DNA fragment from genome of strong virulent GA strain of Marek′s disease herpesvirus (MDV) in lymphoblastoid tumor tissue induced by oncogenic strain Beijing 1 (a specific local strain in China) of MDV. Methods\ Two oligonucleotide primers were synthesized according to the reported sequence of \%meq\% gene an ideal oncogenic candidate and our previously determined sequence of BamHI L fragment of Marek′s disease herpesvirus (MDV), respectively. Reverse transcriptase PCR(RT PCR) assay was performed by using these primers and the mRNA as a template which was isolated from visceral lymphoblastoid tumors obtained from chickens artificially infected with strain Beijing 1 of oncogenic MDV. Southern blot molecular hybridization was further carried out to detect the product of RT PCR with digoxigenin labeled nucleotide probe from BamHI I2 and L fragment in the gene library of MDV strain GA, respectively. Results\ Two probes could simultaneously hybridize this cDNA amplified by RT PCR with a length of about 730 bp. Conclusion\ It is suggested that \%meq\% transcription could extend from the right hand end of BamHI I2 to the adjacent BamHI L, and the BamHI L region was likely to be transcribed in MDV induced lymphoblastoid tumors.展开更多
According to the features of high-resolution panchromatic imagery of Beijing-1 small satellite,an approach to extracting information of residential areas is proposed in this paper based on Gabor texture segmentation.T...According to the features of high-resolution panchromatic imagery of Beijing-1 small satellite,an approach to extracting information of residential areas is proposed in this paper based on Gabor texture segmentation.The algorithm extracts the features in different directions and different scales by building the Gabor filter,uses cluster analysis of multiple features to segment the image,and performs the fusion processing based on morphological scale space.It solves the problems in image processing resulting from low contrast between remote sensing objects and background,the blurring of image edges and high noise.It has the benefits of direction selection and frequency selection with strong self-adaptive ability.Our experiments prove the effectiveness of the approach for extracting information of residential areas from Beijing-1 high-resolution imagery.展开更多
文摘Objective\ To understand the transcription of BamHI L DNA fragment from genome of strong virulent GA strain of Marek′s disease herpesvirus (MDV) in lymphoblastoid tumor tissue induced by oncogenic strain Beijing 1 (a specific local strain in China) of MDV. Methods\ Two oligonucleotide primers were synthesized according to the reported sequence of \%meq\% gene an ideal oncogenic candidate and our previously determined sequence of BamHI L fragment of Marek′s disease herpesvirus (MDV), respectively. Reverse transcriptase PCR(RT PCR) assay was performed by using these primers and the mRNA as a template which was isolated from visceral lymphoblastoid tumors obtained from chickens artificially infected with strain Beijing 1 of oncogenic MDV. Southern blot molecular hybridization was further carried out to detect the product of RT PCR with digoxigenin labeled nucleotide probe from BamHI I2 and L fragment in the gene library of MDV strain GA, respectively. Results\ Two probes could simultaneously hybridize this cDNA amplified by RT PCR with a length of about 730 bp. Conclusion\ It is suggested that \%meq\% transcription could extend from the right hand end of BamHI I2 to the adjacent BamHI L, and the BamHI L region was likely to be transcribed in MDV induced lymphoblastoid tumors.
基金supported by a grant from the National High Technology Research and Development Program of China(863 Program)(No.2005AA133013,No.2006CB701305)the Major State Basic Research Development Program of China(973 Program)(No.2006CB701305).
文摘According to the features of high-resolution panchromatic imagery of Beijing-1 small satellite,an approach to extracting information of residential areas is proposed in this paper based on Gabor texture segmentation.The algorithm extracts the features in different directions and different scales by building the Gabor filter,uses cluster analysis of multiple features to segment the image,and performs the fusion processing based on morphological scale space.It solves the problems in image processing resulting from low contrast between remote sensing objects and background,the blurring of image edges and high noise.It has the benefits of direction selection and frequency selection with strong self-adaptive ability.Our experiments prove the effectiveness of the approach for extracting information of residential areas from Beijing-1 high-resolution imagery.
