A high-performance liquid chromatography (HPLC) method has been developed for the determination of bemegride and its salts in cosmetics. A high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) m...A high-performance liquid chromatography (HPLC) method has been developed for the determination of bemegride and its salts in cosmetics. A high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method was used for the verification of the analytical results. Cosmetic samples were ultrasonically extracted with methanol, centrifuged and filtered by microporous membrane filter prior to instrumental analysis. The chromatographic separation of the analytes was performed using a Symmetry C18 (4.6 mm × 250 mm, 5 μm) column. The quantitation was carried out by external standard method. The experimental results indicated that the limit of quantitation (LOQ) for bemegride and its salts was 0.5 mg/kg. The mean recoveries at three spiked levels ranged from 85.1% to 107.4% with the relative standard deviations (RSDs) between 3.7% and 9.3%. The suspected positive samples were subjected to further verification by HPLC-MS/ MS. The method is accurate, rapid, sensitive and can be used for the inspection of cosmetics.展开更多
文摘A high-performance liquid chromatography (HPLC) method has been developed for the determination of bemegride and its salts in cosmetics. A high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method was used for the verification of the analytical results. Cosmetic samples were ultrasonically extracted with methanol, centrifuged and filtered by microporous membrane filter prior to instrumental analysis. The chromatographic separation of the analytes was performed using a Symmetry C18 (4.6 mm × 250 mm, 5 μm) column. The quantitation was carried out by external standard method. The experimental results indicated that the limit of quantitation (LOQ) for bemegride and its salts was 0.5 mg/kg. The mean recoveries at three spiked levels ranged from 85.1% to 107.4% with the relative standard deviations (RSDs) between 3.7% and 9.3%. The suspected positive samples were subjected to further verification by HPLC-MS/ MS. The method is accurate, rapid, sensitive and can be used for the inspection of cosmetics.
