目的探讨与评价过表达Bestrophin 3对皮质类固醇诱导的股骨头坏死(osteonecrosis of the femoral head,ONFH)保护作用及其机制。方法根据纳入标准和排除标准选取本院于2017年2月至2017年12月期间就诊的ONFH患者术后股骨头骨组织50例作...目的探讨与评价过表达Bestrophin 3对皮质类固醇诱导的股骨头坏死(osteonecrosis of the femoral head,ONFH)保护作用及其机制。方法根据纳入标准和排除标准选取本院于2017年2月至2017年12月期间就诊的ONFH患者术后股骨头骨组织50例作为观察组,股骨颈骨折患者自愿捐赠的股骨头骨组织50例作为对照组,采集两组标本的骨髓基质细胞,使用PCR方法检测骨髓基质细胞Bestrophin 3的表达水平。利用分子生物学手段构建Bestrophin 3不同表达水平的细胞株,通过MTT检测细胞生存率方法比较不同细胞株中细胞生存率。结果观察组中的股骨头骨组织Bestrophin 3相对表达量高于对照组,差异有统计学意义(P<0.05)。高表达Bestrophin 3的细胞株细胞生存率比较高,差异有统计学意义(P<0.05),且细胞生存率与Bestrophin 3表达量呈正相关(P<0.01)。结论Bestrophin 3在皮质类固醇性股骨头坏死骨组织中的呈现高表达状况,Bestrophin 3表达量与细胞生存率呈正相关,高表达Bestrophin 3对细胞具有保护作用。展开更多
目的:探讨与评价Bestrophin 3在激素性股骨头坏死(Osteonecrosis of the femoral head,ONFH)中的作用。方法:研究时间为2017年8月-2018年6月,收集河北医科大学第一医院收治的股骨颈骨折患者自愿捐赠的股骨头骨组织32例作为对照组,激素...目的:探讨与评价Bestrophin 3在激素性股骨头坏死(Osteonecrosis of the femoral head,ONFH)中的作用。方法:研究时间为2017年8月-2018年6月,收集河北医科大学第一医院收治的股骨颈骨折患者自愿捐赠的股骨头骨组织32例作为对照组,激素性股骨头坏死患者术后股骨头骨组织32例作为观察组,采集两组标本的骨髓基质细胞,使用PCR方法检测骨髓基质细胞Bestrophin 3的表达水平。结果:观察组中的股骨头骨组织Bestrophin 3 mRNA相对表达量高于对照组,比较差异有统计学意义(P<0.05)。结论:Bestrophin 3作为一种氯离子通道抑制剂,在激素性股骨头坏死组织中的呈现高表达状况。展开更多
During photosynthesis, photosynthetic electron transport generates a proton motive force （pmf） across the thylakoid membrane, which is used for ATP biosynthesis via ATP synthase in the chloroplast. The pmf is compos...During photosynthesis, photosynthetic electron transport generates a proton motive force （pmf） across the thylakoid membrane, which is used for ATP biosynthesis via ATP synthase in the chloroplast. The pmf is composed of an electric potential （△φ） and an osmotic component （△pH）. Partitioning between these components in chloroplasts is strictly regulated in response to fluctuating environments. However, our knowledge of the molecular mechanisms that regulate pmf partitioning is limited. Here, we report a bestrophin-like protein （AtBest）, which is critical for pmf partitioning. While the △pH component was slightly reduced in atbest, the △φ component was much greater in this mutant than in the wild type, resulting in less efficient activation of nonphotochemical quenching （NPQ） upon both illumination and a shift from low light to high light. Although no visible phenotype was observed in the atbest mutant in the greenhouse, this mutant exhibited stronger photoinhibition than the wild type when grown in the field. AtBest belongs to the bestrophin family proteins, which are believed to function as chloride （Cl^-） channels. Thus, our findings reveal an important Cl^- channel required for ion transport and homeo- stasis across the thylakoid membrane in higher plants. These processes are essential for fine-tuning photosynthesis under fluctuating environmental conditions.展开更多
Objective A calcium-activated chloride current （ICl（Ca）） has been observed in medium-sized sensory neurons of the dorsal root ganglion （DRG）. Axotomy of the sciatic nerve induces a similar current in the majorit...Objective A calcium-activated chloride current （ICl（Ca）） has been observed in medium-sized sensory neurons of the dorsal root ganglion （DRG）. Axotomy of the sciatic nerve induces a similar current in the majority of medium and large diameter neurons. Our aim is to identify the molecule（s） underlying this current. Methods Using conventional and quantitative RT-PCR, we examined the expression in DRG of members of three families of genes, which have been shown to have latch） current inducing properties. Results We showed the detection of transcripts representing several members of these families, i.e. chloride channel calciumactivated （CLCA）, Bestrophin and Tweety gene families in adult DRG, in the normal state and 3 d after sciatic nerve section, a model for peripheral nerve injury. Conclusion Our analysis revealed that that mBestl and Tweety2 appear as the best candidates to play a role in the injury-induced Icl（Ca） in DRG neurons.展开更多
文摘目的:探讨与评价Bestrophin 3在激素性股骨头坏死(Osteonecrosis of the femoral head,ONFH)中的作用。方法:研究时间为2017年8月-2018年6月,收集河北医科大学第一医院收治的股骨颈骨折患者自愿捐赠的股骨头骨组织32例作为对照组,激素性股骨头坏死患者术后股骨头骨组织32例作为观察组,采集两组标本的骨髓基质细胞,使用PCR方法检测骨髓基质细胞Bestrophin 3的表达水平。结果:观察组中的股骨头骨组织Bestrophin 3 mRNA相对表达量高于对照组,比较差异有统计学意义(P<0.05)。结论:Bestrophin 3作为一种氯离子通道抑制剂,在激素性股骨头坏死组织中的呈现高表达状况。
基金supported by the National Natural Science Foundation of China (31322007 and 31570239)the Hundred Talents Program of the Chinese Academy of Sciences
文摘During photosynthesis, photosynthetic electron transport generates a proton motive force （pmf） across the thylakoid membrane, which is used for ATP biosynthesis via ATP synthase in the chloroplast. The pmf is composed of an electric potential （△φ） and an osmotic component （△pH）. Partitioning between these components in chloroplasts is strictly regulated in response to fluctuating environments. However, our knowledge of the molecular mechanisms that regulate pmf partitioning is limited. Here, we report a bestrophin-like protein （AtBest）, which is critical for pmf partitioning. While the △pH component was slightly reduced in atbest, the △φ component was much greater in this mutant than in the wild type, resulting in less efficient activation of nonphotochemical quenching （NPQ） upon both illumination and a shift from low light to high light. Although no visible phenotype was observed in the atbest mutant in the greenhouse, this mutant exhibited stronger photoinhibition than the wild type when grown in the field. AtBest belongs to the bestrophin family proteins, which are believed to function as chloride （Cl^-） channels. Thus, our findings reveal an important Cl^- channel required for ion transport and homeo- stasis across the thylakoid membrane in higher plants. These processes are essential for fine-tuning photosynthesis under fluctuating environmental conditions.
文摘Objective A calcium-activated chloride current （ICl（Ca）） has been observed in medium-sized sensory neurons of the dorsal root ganglion （DRG）. Axotomy of the sciatic nerve induces a similar current in the majority of medium and large diameter neurons. Our aim is to identify the molecule（s） underlying this current. Methods Using conventional and quantitative RT-PCR, we examined the expression in DRG of members of three families of genes, which have been shown to have latch） current inducing properties. Results We showed the detection of transcripts representing several members of these families, i.e. chloride channel calciumactivated （CLCA）, Bestrophin and Tweety gene families in adult DRG, in the normal state and 3 d after sciatic nerve section, a model for peripheral nerve injury. Conclusion Our analysis revealed that that mBestl and Tweety2 appear as the best candidates to play a role in the injury-induced Icl（Ca） in DRG neurons.
