Lower Bifidobacteria counts in both duodenal mucosa-associated and fecal microbiota in irritable bowel syndrome patients

AIM: To determine the composition of both fecal and duodenal mucosa-associated microbiota in irritable bowel syndrome (IBS) patients and healthy subjects using molecular-based techniques. METHODS: Fecal and duodenal mucosa brush samples were obtained from 41 IBS patients and 26 healthy subjects. Fecal samples were analyzed for the composition of the total microbiota using fluorescent in situ hybridization (FISH) and both fecal and duodenal brush samples were analyzed for the composition of bif idobacteria using real-time polymerase chain reaction. RESULTS: The FISH analysis of fecal samples revealed a 2-fold decrease in the level of bifidobacteria (4.2 ± 1.3 vs 8.3 ± 1.9, P < 0.01) in IBS patients compared to healthy subjects, whereas no major differences in other bacterial groups were observed. At the species level, Bifidobacterium catenulatum levels were significantly lower (6 ± 0.6 vs 19 ± 2.5, P < 0.001) in the IBS patients in both fecal and duodenal brush samples than in healthy subjects.CONCLUSION: Decreased bifidobacteria levels in both fecal and duodenal brush samples of IBS patients compared to healthy subjects indicate a role for microbiotic composition in IBS pathophysiology.

Anti-inflammatory effects of bifidobacteria by inhibition of LPS-induced NF-κB activation

AIM： Different strains of bifidobacteria were analysed for their effects on HT-29 intestinal epithelial cells （IECs） in in vitro models both of the non-inflamed and inflamed intestinal epithelium. METHODS： A reporter gene system in HT-29 cells was used to measure levels of NF-KB activation after challenge with bifidobacteria or after bacterial pre-treatment following LPS challenge. IL-8 protein and pro-inflammatory gene expression was investigated using normal HT-29 cells. RESULTS： None of the bifidobacteria tested induced activation of nuclear factor κB （NF-κB） indicating that bifidobacteria themselves do not induce inflammatory events in IECs. However, six out of eight bifidobacteria tested inhibited lipopolysaccharide- （LPS-） induced NF-κB activation in a dose- and strain-dependent manner. In contrast, NF-κB activation in response to challenge with tumor necrosis factor-α （TNF-α） was affected by none of the tested bifidobacteria, indicating that the inhibitory effect of bifidobacteria is specific for LPS-induced inflammation in IECs. As shown with two of the six inhibitionpositive bifidobacteria, LPS-induced inhibition of NF-κB activation was accompanied by a dose-dependent decrease of interleukin 8 （IL-8） secretion and by lower mRNA levels for IL-8, TNF-α, cyclooxygenase 2 （Cox-2）, and intercellular adhesion molecule 1（ICAM-1）. CONCLUSION： Some strains of bifidobacteria are effective in inhibiting LPS-induced inflammation and thus might be appropriate candidates for probiotic intervention in chronic intestinal inflammation.

Effect of EPEC endotoxin and bifidobacteria on intestinal barrier function through modulation of toll-like receptor 2 and toll-like receptor 4 expression in intestinal epithelial cell-18

AIM To investigate toll-like receptor 2(TLR2) and TLR4 expression, following bifidobacteria and low-dose EPEC endotoxin treatment, and intestinal barrier function in rat intestinal epithelial cell-18(IEC-18).METHODS Six experimental groups were established-normal control, EPEC, Bifidobacteria infantis(B. infantis), B. longum, B. bifidum, and B. youth groups. Optimal EPEC endotoxin concentration, bifidobacteria fold dilution, and treatment duration were determined. Quantitative real-time polymerase chain reaction and western blot, respectively, were conducted to detect TLR2 and TLR4 m RNA and protein expression in IEC-18 cells. Transepithelial electrical resistance(TEER) was measured by the EVOM chopstick voltohmmeter in each group. All experiments were conducted in triplicate and data were analyzed on SPSS 16.RESULTS TLR2 and TLR4 m RNA and protein expression in the EPEC group were significantly higher than in the control group(P < 0.05). TLR2 m RNA and protein expression in the B. infantis, B. longum and B. youth groups were significantly lower than in the normal control group(P < 0.05). TLR4 m RNA and protein expression in the B. bifidum and B. youth groups were significantly lower than in normal controls(P < 0.05). In addition, the TEER in B. infantis, B. longum, B. bifidum, and B. youth groups were decreased by 19%, 18%, 23% and 23%, respectively, after 120 min of intervention, as compared to the control group. However, the TEER in the EPEC group was significantly decreased by 67% in comparison to the normal control group(P < 0.05).CONCLUSION Bifidobacteria protect IEC-18 cells against injury by down-regulating TLR2 and TLR4 expression and enhance intestinal barrier function to protect the intestinal epithelial cells from pathogenic invasion.

Bifidobacteria as Potential Functional Starter Cultures: A Case Study by MSc Students in Food Science and Technology (University of Foggia, Southern Italy)

This research paper was the results of activity of MSc students of Food Science and Technology, attending the class “Biotechnology of Functional Starter”. Five strains of bifidobacteria (Bifidobacterium animalis subsp. lactis;B. longum subsp. infantis;B. breve;B. animalis subsp. animalis;B. bifidum) were evaluated in order to assess their suitability as functional starter cultures, by studying the following technological and probiotic traits: growth at different temperatures, NaCl amounts and pH values;acidifying ability;metabolism (arginin deamination, esculin hydrolysis, acetoin production);survival at low pH and in presence of bile salts;hydrophobic properties;antibiotic resistance. After laboratory assays and strain selection through a multivariate analyses, it was highlighted that B. longum subsp. infantis and B. animalis subsp. lactis represent a good compromise as potential functional starter cultures, as B. animalis subp. lactis showed a high growth index at pH 5 and good values at 25?C and 30?C, as well as the minimal viability loss at pH 2.5. B. longum subsp. infantis DSMZ 20088 was the best microorganism for its growth index in presence of 6.5% of salt added and at 25?C and 30?C.

SIGNAL MECHANISM OF INHIBITION OF BIFIDOBACTERIA ON GROWTH OF COLON CANCER

Objective: To explore the antitumor mechanisms of bifidobacteria adolescence in vivo. Methods: The content of extracellular signal regulated proteins (ERK)1/2, C-Jun N-terminal kinase (JNK), p38, c-fos and c-jun in nude mouse transplanted large bowel carcinoma was detected by using laser confocal microscopy. The expression of NF-κB was determined by immunohistochemistry. Results: After the nude mouse transplanted tumor was treated with bifidobacteria, the average fluorescent strength of ERK1/2, JNK, c-fos and c-jun was significantly lower than that in tumor control group (P<0.01). The average fluorescent strength of p38 was not obvious difference in the two groups (P>0.05). The positive cell density of NF-κB in large bowel carcinoma transplantation tumors in Bifidobacterium injection group was markedly lower than that in tumor group(P<0.01). Conclusion: bifidobacteria adolescence could markedly decrease the activity of ERK1/2 and JNK, the expression c-fos and c-jun, and the activity of NF-κB.

Effects of Fructooligofructoses Chain Length on the Bifidobacteria of the Human Colon: A Pilot Study

Human gastrointestinal health may be improved by the consumption of prebiotic food ingredients such as fructooligo-fructoses. A study was initiated to determine the effect of fructooligofructoses of different chain lengths on gastrointes-tinal parameters. Nineteen healthy subjects aged 20 - 57 y took part in a 10-week cross-over designed study. Subjects consumed either inulin or oligofructose for 3 weeks followed by a 2-week washout period between treatments. Stool samples were collected five times (baseline, 2 treatments, 2 washout) and analyzed for bifidobacteria. Daily records were kept for stool frequency, stool consistency and flatulence frequency. Bifidobacteria counts (cfu/ml) were higher (trending toward significance) during inulin and oligofructose intakes (1.2 × 107 ± 4.8 × 107 and 2.0 × 108 ± 4.7 × 108) and washout periods (2.9 × 106 ± 6.5 × 106 and 1.1 × 107 ± 1.6 × 107) than baseline counts (2.2 × 105 ± 5.1 × 105 and 2.9 × 106 ± 6.5 × 106), respectively. Inulin and oligofructose treatment periods had a significant effect on stool consistency (watery/very hard) and flatulence frequency, but not stool frequency, when compared to baseline (P < 0.05). Further research is needed to confirm these results due to small sample size and the need for a longer washout period between treatments.

Dietetic Valorization of Cow＇s Milk Proteins Fermented at 45 ℃ by Lactobacillus acidophilus Associated with Bifidobacteria

Cow＇s milk constitutes a significant food for babies when mother＇s milk is insufficient. The purpose of this work is to offer a fermented milk, potentially hypoallergenic, using selected Lactobaeillus acidophilus and Bifidobacteria sp. for their proteolytic activities. The fermentation is evaluated by the rate of lactic acid production and by the bacterial enumeration at 45 ℃. The rate of acidification obtained by mixed cultures （La ＋ B. longum, 63.8 ± 3.5 °D） and （La ＋ B. breve, 43.4 ± 1.67 °D） compared with the control （milk） （18.6 ± 1.31 °D）. The result of the best hydrolysis was obtained by （La＋ B. longum） （154.88 ± 30.33 ug/mg） which corresponded to a better release of the a-NH2 functions （103.32 ± 12.81 umoles/mg） compared with control （307.2 ± 11.54 ug/mg and 10.25 ± 0.44 umoles/mg ）. The best synergy was obtained by （La ＋ B. bifidum） （13 × 10^6 cfu/mL and 60 × 10^6 cfu/mL） and reached 95 × 10^10 cfu/mL and 119 × 10^10 cfu/mg at the end. The electrophoresis of fermented milk revealed the presence of soluble proteins （a-Lactalbumin and β-Lactoglobulin）. The Enzyme Linked Immtmo Sorbent Assay showed the results of residual antigenic activities of β-Lg （1.4 ± 0.23 ug/mg）, a-La （0.012 ± 0.004 ug/mg） and bovin serum albumin （0.014 ± 0.005 ug/mg）by the associations （La ＋ B. longum）, （La ＋ B. bifidum） and （La ＋ B. bifidum） compared with the control （14.43 ± 5.91 ug/mg, 0.183 ± 0.062 gg/mg, 0.05 ± 0.008 ug/mg） respectively. Lactic fermentation reduced to a significant antigenic reactivity of principal whey milk proteins.

Deep Exploration of Bifidobacteria through Metabolomics Study

Bifidobacteria are probiotic bacteria with multiple health-promoting properties for human being. The global market for probiotics, especially for bifidobacteria is booming. However, the entire market is still at an early stage as there is nearly no fine products developed yet except the whole bacterial cells. The maturation of metabolomics technologies make it possible to study complex mixture with high-throughput, comprehensive maps and libraries. Therefore, we prospect that metabolomics studies mainly based on liquid/gas chromatography-mass spectrometry (LC/GC-MS) can deepen our understanding in detail during the study of metabolic mechanisms of bifidobacteria. These studies can be conducted at three phases, including non-targeted, targeted metabolomic analysis of bifidobacteria, and specific metabolites production through metabolic engineering and fermentation. Metabolomic studies of bifidobacteria will allow us to fully explore their metabolic mechanisms and to utilize metabolites that contribute to human health. In particular, bifidobacteria derived conjugated linoleic acids and bacteriocins are two kinds of fined products that may have great potentials in the future and can be used as food additives.

Functional Characterization of Bifidobacteria of Human Origin: A Case Study by the Students of Food Science and Technology of the University of Foggia (Southern Italy)

The aim of this paper was to study the potential technological and probiotic properties of bifidobacteria isolated from human feces. Bifidobacteria, naturally present in the dominant colonic microbiota, represent up to 25% of the cultivable faecal bacteria in adults and 80% in infants. Bifidobacteria have been shown to adhere and colonize in high numbers different types of cultured intestinal epithelial cells;moreover some authors reported that some strains are able to stabilize the intestinal microbiota during and after antibiotic therapy, modulate the immune system, protecting against chemically induced intestinal inflammation and reducing symptoms of colitis. Eight isolates of bifidobacteria were studied to assess their technological and probiotic traits;the technological characterization relied on the assessment of enzymatic activities (proteolytic and lipolytic activity), growth under various conditions (pH, temperature and addition of salt), acidifying ability and metabolism (arginine deamination, esculin, esculin hydrolysis and citrate metabolism). The study of the probiotic characteristics focused on the evaluation of the survival at low pH and with bile salts added, antibiotic resistance, and hydrophobic properties. As a result of this process, two promising strains were selected for further studies.

Bifidobacteria Upregulate Expression of Toll-Like Receptor Negative Regulators Counteracting Enterotoxigenic <i>Escherichia coli</i>Mediated Inflammation in Bovine Intestinal Epitheliocytes

We previously established a bovine intestinal epithelial cell line (BIE cells) and showed that BIE cells are useful in vitro model system for the study of interactions between pathogenic and beneficial microorganisms and bovine intestinal epithelial cells (IECs). In the present study, we aimed to select potential immunomodulatory bifidobacteria that may be used to beneficially modulate the inflammatory response in bovine IECs. We also aimed to gain insight into the molecular mechanisms involved in the anti-inflammatory effect of bifidobacteria by evaluating the role of Toll-like receptor (TLR)-2 and TLR negative regulators in the regulation of proinflammatory cytokines production and MAPK, NF-κB and PI3K pathways activation in BIE cells. Five bifidobacteria strains were evaluated in this study and according to their capacity to modulate the inflammatory response of BIE cells. Despite the unique effect of each strain, four common points were found when comparing the effect of the high and moderate anti-inflammatory strains: 1) Upregulation of TLR negative regulators and the intensity of that upregulation was related to the different immunomodulatory capacity of each bifidobacteria strain;2) The balance between MAPK activation and MKP-1 upregulation affected the anti-inflammatory effect of bifidobacteria in BIE cells;3) The inhibition of PI3K pathway was related to the anti-inflammatory effect of bifidobacteria;4) The immunoregulatory effect of bifidobacteria in BIE cells is partially dependent on TLR2. This study shows that BIE cells can be used for the selection of immunoregulatory bifidobacteria and for studying the mechanisms involved in the protective activity of immunobiotics against TLR4-induced inflammatory damage. In addition, we have demonstrated that the anti-inflammatory effect of bifidobacteria was achieved by a complex interaction of multiple TLRs negative regulators as well as the inhibition/activation of multiple signaling pathways.

Bioinformatic Survey of S-Layer Proteins in Bifidobacteria

Surface layer (S-layer) proteins are one of the most commonly observed cell envelope components in both Archaea and Bacteria. It has versatile functions and holds considerable application potential in biotechnology. Bifidobacteria are representative probiotics conferring health promoting properties. However, there is little study of S-layer in bifidobacteria yet. The distribution and characteristics of S-layer in bifidobacteria are unknown. In this study, search for S-layer protein in the identical protein groups in NCBI yielded 49 hits belonging to bifidobacteria. These proteins were annotated as either “S-layer (domain) protein” or “putative S-layer (y) domain protein” that distributed among 26 species of Bifidobacterium genus. Multiple alignments suggest S-layer proteins are relatively conservative. Phylogenetic analysis of 24 S-layer (domain) protein sequences groups them into three distinct clusters, with the majority species in Cluster-2. S-layer (domain) protein has a universe motif DUF4381, though its function is unknown. Meanwhile, two other motifs CARDB and EphA2_TM involved in cell adhesion and cell signaling respectively, presented in most S-layer (domain) protein in bifidobacteria. All S-layer proteins have a typical N-terminal Sec-dependent signal peptide and a C-terminal trans-membrane region. Homological modeling of representative S-layer proteins from each cluster revealed a few unique structural features. All representative S-layer proteins have a plenty of β-meander motif that exclusively composed by β-barrel structural architectures linked together by hairpin loops.

Research progress in the use of bifidobacteria for the treatment of colorectal cancer

Colorectal cancer has high morbidity and mortality rates;therefore,developing new therapeutic drugs and methods for it is crucial.There is increasing evidence that intestinal flora plays a vital role in maintaining intestinal homeostasis and therefore,in the development of colorectal cancer.As such,therapeutic approaches using probiotics such as Bifidobacterium lactis to regulate intestinal flora are expected to represent a new treatment strategy for colorectal cancer.This article introduces and compares the development status of conventional therapies for colorectal cancer,primarily targeted therapy and immunotherapy,the relationship between intestinal flora and colorectal cancer,and the research and application of Bifidobacterium bifidum in colorectal cancer treatment to provide a reference for its clinical application.

Viability of Bifidobacteria in frozen yogurt made from camel milk

The purpose of this research was to incorporate Bifidobacterium angulatum and Bifidobacterium infantis in frozen fermented dairy desserts made from camel or cow milk and to determine their viability during freezing and storage at .20℃. To meet this objective, ice cream mixtures were formulated using camel or cow milk constituents, inoculated with regular yogurt starter （Lactobacillus delbruecldi ssp bulgaricus and streptococcus thermophilus） and incubated at 42℃ till a pH value of 5.0 was attained. The fermented mixes were heated to 80℃ for 5 min in water bath to inhibit yogurt organisms. Bifidobacteria were then added at 2 g/kg mix （1 gram from each Bifidobacterium strain）. The results showed that the initial counts of Bifidobacteria before freezing were 7.3 × 10^8 and 7.1 × 108 cfu/g for camel and cow mix respectively and decreased to 1.06× 10^8 and 1.1×10^8 in the same order （about 0.8 log reduction in the count of Bifidobacteria was observed） after freezing and storage for one day. No significant changes in counts of Bifidobaeteria were found during storage at -20℃ for 17 weeks. Changes in pH and titratable acidity were also studied. No significant changes in titratable acidities of frozen yogurt made from camel or cow milk constituents during storage period were observed.

Effects of Bifidobacteria bifidum strains on 2,4,6-trinitrobenzene sulfonic acid (TNBS)-induced acute colitis and its potential mechanism

Microbiota-based therapies for improving symptoms of Crohn’s disease(CD)are receiving increasing attention.Probiotics and second-generation probiotics can potentially prevent this disease.However,the therapeutic action and underlying molecular mechanisms of probiotics remain largely unknown.Efficient screening of probiotics is also challenging.In this study,we screened eight B.bifidum strains with CD-relieving potential using a 2,4,6-trinitrobenzene sulfonic acid(TNBS)colitis mouse model.We also observed that B.bifidum CJ238 could prevent but not cure the colitis-symptoms(weight loss,colon shortening and connective tissue hyperplasia)in mice model.The 16S rRNA sequencing data showed that supplementation with B.bifidum did not alter the composition of the intestinal microbiota.B.bifidum CJ238 administration also provided full protection against acute colitis in pseudo germ-free mice.Therefore,we infered that the protective effect of B.bifidum strains does not depend on their interaction with the gut microbiota.Furthermore,heat-killed(95℃ for 20min)B.bifidum cells(109 CFU/mouse)also protected mice against acute colitis.In-vitro adhesion and immunomodulatory activities of the B.bifidum strains were measured.Combined with the in-vivo results,the anti-colitis effect of B.bifidum was positively associated with its strong anti-inflammatory and adhesion capacity in-vitro.This study provides insights into the in-vitro screening and clinical application of probiotics in CD prevention.

Bifidobacteria DNA Induces Murine Macrophages Activation in vitro

Previous studies have shown that oligodeoxynucleotides containing unmethylated CpG motifs were used as adjuvants for immunoregulation and immune response. This study was to explore the activation effects of Bifidobacteria DNA containing unmethylated CpG motifs （CpG DNA） on murine macrophage J774A.1 cells. The genomic DNA of Bifidobacteria was extracted and purified, and the methylation degree of CpG motifs was tested. The phagocytic ability of the macrophages was detected by flow cytometry. The cytokines （IL-1β, IL-6, IL-12p40 and TNF-α） levels in the culture supernatants of Bifidobacteria DNA treated J774A.1 cells were assayed by ELISA. The content of nitric oxide （NO） was detected by Griess reagent. After treated with Bifidobacteria DNA for 24h, Nile Red stain increased in J774A.1 macrophage, which suggested that the lipid metabolism increased in the macrophages. The phagocytic ability and levels of NO and cytokines of IL-1β, IL-6, IL-12p40 and TNF-α were significantly higher than PBS group and CT DNA group. The results indicated that Bifidobacteria DNA could activate murine macrophages J774A.1, which could provide scientific basis for the research and application of microorganism DNA preparation. Cellular ＆ Molecular Immunology. 2005;2（6）：473-478.

A preliminary clinical study of bifidobacteria preparation on the treatment of diarrhea in severely burned patients

Objective To investigate the role of bifidobacteria preparation in the prevention of diarrhea in severely burned patients. Methods Forty three severely burned patients who were inflicted by diarrhea were included in this study. The changes of intestinal microflora were observed. Results The intestinal flora of all patients with diarrhea changed greatly before treatment. The proportions of bifidobacteria and bacteroid decreased significantly, whereas those of aerobe and Candida increased relatively. The ratio of anaerobe to aerobe decreased. As a result, the patients' intestinal flora were distorted. After six days treatment of bifidobacteria, diarrhea in most patients ceased and the intestinal microflora restored. Conclusion Bifidobacteria feeding plays an important role in restoring intestinal microflora and stopping diarrhea in severely burned patients.

Bifidobacteria and host health——an update

The intestinal microbiota has a vital role in human health.Especially,Bifidobacteriumis a genus that is crucial for health and different diseases.Further potentially functional investigation of Bifidobacteriumis needed,which will be of great importance to human health.This review aims to describe bifidobacteria compositional changes associated with different ages,to highlight the impact of bifidobacteria on health and disease,and to indicate their clinical applications.Bifidobacterium longum,B.breve,and B.bifidumare dominant in infants,whereas B.catenulatumand B.adolescentis are more prevalent in adults.Bifidobacteria improve the treatment of diarrhea,inflammatory bowel disease(IBD),colon irregularity,colorectal cancer,necrotizing enterocolitis,diabetes and disorder of carbohydrate metabolism.

双歧杆菌缓解溃疡性结肠炎的作用机制研究进展

溃疡性结肠炎是一种慢性炎症性肠病,其发病率在我国逐年升高。目前的药物治疗方式均存在一定的副作用,利用益生菌调节肠道菌群成为一种新兴干预方式。大量研究表明,双歧杆菌具有很好的缓解溃疡性结肠炎症状的临床效果。然而,双歧杆菌缓解溃疡性结肠炎的作用机制还没有较为系统深入的阐述。近年来,随着多组学、肠道菌群、肠-脑轴、肠-肝轴相关研究的不断突破,双歧杆菌缓解溃疡性结肠炎的作用机制研究也取得了重要进展。本文综述了近10年双歧杆菌缓解溃疡性结肠炎在研究和应用方面取得的最新进展,介绍了具有缓解溃疡性结肠炎作用的双歧杆菌,并主要对其缓解机制和研究应用进行归纳总结,阐释了双歧杆菌主要通过调节肠道菌群结构、修复肠道屏障、改善宿主肠道免疫反应、调节肠道嘌呤代谢、抑制氧化应激和为肠上皮提供能量等作用缓解溃疡性结肠炎,以期为进一步阐明双歧杆菌对生命健康的调控机制及益生菌制剂的进一步应用推广提供参考。

基于脑肠轴理论探讨双歧杆菌联合中药肉苁蓉对于帕金森病患者抑郁及痴呆改善的调控作用

目的:探讨双歧杆菌联合中药肉苁蓉对于帕金森病患者抑郁及痴呆改善的调控作用,以及其可能的机制。方法:选取2022年1月-2023年2月在本院诊治的帕金森病(PD)患者112例,按照随机数字表法分为两组,每组56例。对照组给予常规治疗,实验组在对照组基础上加用双歧杆菌联合中药肉苁蓉治疗,观察并记录患者的一般资料、临床症状、抑郁程度、认知功能、血清神经营养因子(BDNF)、肠道菌群结构等指标。结果:治疗后,两组患者的运动症状均有不同程度的改善,但实验组的改善幅度更大,即统一帕金森病评定量表(UPDRS)总分和各分项评分均低于对照组(P<0.05)。实验组患者的抑郁程度和认知功能改善也明显优于对照组,即Hamilton抑郁量表(HAMD)评分和Montreal认知评估量表(MoCA)评分优于对照组(P<0.05)。实验组患者的血清BDNF水平显著高于对照组(P<0.05),提示神经保护作用增强。实验组患者的肠道菌群结构也发生了有利的变化,与对照组相比,肠道中有益菌如双歧杆菌、乳酸杆菌等比例增加,而有害菌如厌氧菌、产氢硫菌等比例减少(P<0.05)。结论:双歧杆菌联合中药肉苁蓉能够有效改善帕金森病患者的运动症状、抑郁程度和认知功能,其机制可能与增强神经保护作用和调节肠道菌群失衡有关。该方法为帕金森病的综合治疗提供了一种新的思路和手段。

双歧杆菌四联活菌片联合恩替卡韦对老年乙肝肝硬化患者肝纤维化程度及IL-10、sICAM-1水平的影响

目的探讨双歧杆菌四联活菌片+恩替卡韦治疗老年乙肝肝硬化(hepatitis B cirrhosis,HBC)患者的效果及对肝纤维化程度、IL-10、sICAM-1水平的影响。方法选取我院2021年4月至2023月6月收治的老年HBC患者一共86例,随机分为观察组、对照组,每组43例。观察组采用双歧杆菌四联活菌片联合恩替卡韦治疗,对照组采用恩替卡韦治疗。比较2组疗效、治疗前、治疗6个月后肝功能指标[天门冬氨酸氨基转移酶(AST)、丙氨酸氨基转移酶(ALT)、总胆红素(TBIL)]、肝纤维化指标[层粘连蛋白(LN)、Ⅲ型前胶原(PCⅢ)、透明质酸(HA)、Ⅳ型胶原(Ⅳ-C)]、炎性因子指标[可溶性细胞间黏附分子-1(sICAM-1)、白细胞介素-10(IL-10)]水平、肠道菌群指标[乳酸杆菌、肠杆菌、双歧杆菌]数量。结果观察组总有效率高于对照组(P<0.05);治疗6个月以后观察组ALT、TBIL、AST水平均低于对照组(P<0.05);治疗6个月以后观察组LN、HA、Ⅳ-C、PCⅢ水平均低于对照组(P<0.05);治疗6个月以后sICAM-1水平低于对照组,观察组IL-10水平高于对照组(P<0.05)。结论双歧杆菌四联活菌片联合恩替卡韦治疗老年HBC患者效果确切,可有效调节肠道菌群,纠正免疫炎症反应,改善肝功能,减轻肝纤维化,利于控制病情进展。