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The principle of optimization of binary mobile phase composition of multi-step linear gradient elution
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作者 ZOU Han-Fa ZHANG Yu-Kui +2 位作者 DONG Li-Fu BAO Mian-Sheng LU Pei-Zhang 《Acta Chimica Sinica English Edition》 SCIE CAS CSCD 1989年第6期511-519,共1页
The basic principle of optimal method called “moving overlapping resolution mapping Method” to select the optimal binary mobile phase composition of multi-step linear gradient liquid chromatography is discussed with... The basic principle of optimal method called “moving overlapping resolution mapping Method” to select the optimal binary mobile phase composition of multi-step linear gradient liquid chromatography is discussed with simultaneously considering effects of position of solute inside the column and mobile phase composition on peak resolution and retention value, then a BASIC program based on this principle is developed in IBM-PC computer. The validities of both principle of optimization and BASIC program are confirmed by separation of samples Containing bile acids and PAHs in RP-HPLC. 展开更多
关键词 time LENGTH The principle of optimization of binary mobile phase composition of multi-step linear gradient elution
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