A comparative study of the biochemical and biophysical characteristics of myocardial mitochondria was conducted in 16-weeks-old male stroke-prone spontaneously hypertensive rats （SHRsp） and normotensive Wistar-Kyoto...A comparative study of the biochemical and biophysical characteristics of myocardial mitochondria was conducted in 16-weeks-old male stroke-prone spontaneously hypertensive rats （SHRsp） and normotensive Wistar-Kyoto control rats （WKY） （n = 6-12）. Cardiac and ventricular weights of SHRsp were significantly higher than those of the WKY group. The average amounts of mitochondria （Mit） and sarcoplasmic reticulum （SR） proteins isolated from the ventricle of SHRsp were 37% and 25% greater, respectively, when compared with those of WKY. Mit cytochrome c oxidase activities （μmol / min / mg） were 4.63 ± 0.71 for SHRsp and 5.55 ± 0.50 for WKY myocardium （x± s）, so that the activity of the former was significantly reduced by 17% （P<0.01）. Mit Ca2- , Mg2-TPase activity （μmol / min / mg） of SHRsp was 24% lower than that of WKY myocardium （P<0.01）. The fluorescence polarization of myocardial Mit membrane labelled with DPH （1, 6-diphenyl-1, 3, 5-hexatriene） in hypertensive rats was not different from that in con展开更多
基金This work was supported In part by the program re-search grant 85-915-03-01 lfrom the StuteCommlsslon of Science and Technology of China.
文摘A comparative study of the biochemical and biophysical characteristics of myocardial mitochondria was conducted in 16-weeks-old male stroke-prone spontaneously hypertensive rats （SHRsp） and normotensive Wistar-Kyoto control rats （WKY） （n = 6-12）. Cardiac and ventricular weights of SHRsp were significantly higher than those of the WKY group. The average amounts of mitochondria （Mit） and sarcoplasmic reticulum （SR） proteins isolated from the ventricle of SHRsp were 37% and 25% greater, respectively, when compared with those of WKY. Mit cytochrome c oxidase activities （μmol / min / mg） were 4.63 ± 0.71 for SHRsp and 5.55 ± 0.50 for WKY myocardium （x± s）, so that the activity of the former was significantly reduced by 17% （P<0.01）. Mit Ca2- , Mg2-TPase activity （μmol / min / mg） of SHRsp was 24% lower than that of WKY myocardium （P<0.01）. The fluorescence polarization of myocardial Mit membrane labelled with DPH （1, 6-diphenyl-1, 3, 5-hexatriene） in hypertensive rats was not different from that in con
