Magnetic Resonance imaging (MR imaging) as a powerful non-invasive modality is of high global interest for early cancer detection. The aim of this study was the synthesis of nanodendrimer and its conjugate with monocl...Magnetic Resonance imaging (MR imaging) as a powerful non-invasive modality is of high global interest for early cancer detection. The aim of this study was the synthesis of nanodendrimer and its conjugate with monoclonal antibody C595 against breast cancer cell, followed by its chelating with gadolinium for its magnetic property. First, anti-MUC-1 monoclonal antibody C595 was coupled to a biodegradable biocompatible Anionic Linear Globular Dendrimer G2 (having polyethylene glycol core and citric acid shell). Then prepared nanocomplex loaded by gadolinium to make novel agent of functional MR imaging. Anticancer effects and MR imaging parameters of the prepared nanoconjugate was investigated under in vitro conditions doing performing several studies such as evaluation of monoclonal antibody C595 binding to mucine-1 (MUC-1) cell, its purification, size of nanoconjugate and relaxivity measurements. The obtained data showed a powerful relaxations as well as selective MUC-1 antigen binding to the cell. Based on the findings from the present research Gd3+-ALGDG2-C595 nano-probe may be a potential breast molecular imaging and therapeutic agent. However, further investigations by in vivo studies and clinical trials are in the pipeline.展开更多
Liposomes are one of the significant classes of antitumor nanomaterials and the most successful nanomedicine drugs in clinical translation. However, it is difficult to accurately reveal liposome delivery modes and dru...Liposomes are one of the significant classes of antitumor nanomaterials and the most successful nanomedicine drugs in clinical translation. However, it is difficult to accurately reveal liposome delivery modes and drug release rates at different p H values to assess the biodistribution and drug delivery pathways in vivo. Here, we established a strategy to integrate Bi-doped carbon quantum dots(CQDs)with liposomes to produce fluorescence visualization and therapeutic effects, namely lipo/Bi-doped CQDs.Lipo/Bi-doped CQDs show good water solubility and physicochemical properties, which can be used for in vitro labeling of colon cancer(CT26) cells and in vivo imaging localization tracking tumors for monitoring. Simultaneously, thanks to the excellent p H sensitivity and ion doping characteristic of Bi-doped CQDs, lipo/Bi-doped CQDs can be used to reveal the drug release rate of liposomes at different p H values and exhibit potential effects in vivo antitumor therapy.展开更多
To investigate the validity and accuracy of tissue Doppler imaging (TDI) using a novel balloon phantom, validation of TDI myocardial velocity measurements has been carried out indirectly from conventional M mode imag...To investigate the validity and accuracy of tissue Doppler imaging (TDI) using a novel balloon phantom, validation of TDI myocardial velocity measurements has been carried out indirectly from conventional M mode images. However it is not a true and independent gold standard. We described a new TDI validation method by using a specially developed left ventricular balloon model mounted in a water bath and constructed using two pear shaped balloons. It was connected to a pulsatile flow pump at 8 stroke volumes (50-85 ml/beat). The displacement and velocity of the balloon walls were recorded simultaneously by video imaging and TDI on a GE Vingmed System Five with a 5 MHz phased array probe at the highest frame rates available. Conventional M mode and 2 D imaging verified that our balloon model mimicked the shape and wall motion of left ventricle. There was a good correlation and agreement between the maximum video excursion of the anterior and posterior walls of the phantom and the results of the temporal integration of digital distance data by TDI (Anterior wall: r =0.97, SEE=0.24 mm, ±s =0.04±0.24 mm; Posterior wall: r =0.95, SEE=0.22 mm, ±s =0.03±0.24 mm). Analysis of the velocity profile by the TDI method showed that the velocity at each measured point was correlated well with the velocity obtained from the video images (Anterior wall: r =0.97, SEE=0.30 mm, ±s =-0.04±0.28 mm; Posterior wall: r =0.97, SEE=0.30 mm, ±s =0.04±0.28 mm). Our balloon model provided a new independent method for the validation of TDI data. This study demonstrated that the present TDI system is reliable for measuring wall motion distance and velocity.展开更多
A simple and rapid method was established to study vascular permeability by in vitroperfused endothelial cell monolayers cultured on micropore filter membrane.It can be used todetermine filtration coefficient （K<s...A simple and rapid method was established to study vascular permeability by in vitroperfused endothelial cell monolayers cultured on micropore filter membrane.It can be used todetermine filtration coefficient （K<sub>f</sub>） to small molecules and osmotic reflection coefficient （σ） toproteins of the endothelial monolayer.Hanks’ balanced salt solution （HBSS） or 5g/L albuminin HBSS was used to perfuse the confluent endothelial monolayer at the hydrostatic pressure of2.45kPa （25cm H<sub>2</sub>O）.Control K<sub>f</sub> values were 10.1±0.75 and 3.6±0.75μl·min<sup>-1</sup>·cm<sup>-2</sup>·kPa（-1）（±,n=3） respectively for the perfusion of HBSS and albumin HBSS,suggesting that al-bumin may decrease endothelial monolayer permeability to water and small molecules.After ex-posure of endothelial monolayer to 10<sup>-8</sup>mol/L platelet-activating factor （PAF） for 30min,K<sub>f</sub>values increased to 193.1% and 133.3% respectively.Protein clearance rate (μl.min<sup>-1</sup>·cm<sup>-2</sup>)and osmotic reflection coefficient of the control endothelial monolayer were 8.0±3.22 and 0.37±0.09 respectively and those of the PAF treated endothelial monolayer 12.2±2.95μl·min<sup>-1</sup>·cm<sup>-2</sup> and 0.18±0.06,revealing increased permeability to albumin.Computer-assisted imageprocessing demonstrated that PAF treatment decreased cell area while increased cell form factorand intercellular space,suggesting that endothelial cells retracted and rounded,which may bean important mechanism of PAF-induced increase of vascular permeability.展开更多
In the procerebrum (PC), the olfactory center, of the land slug Limax, an oscillation of local field potential (LFP) with 0.5 - 1 Hz is observed by electrophysiological extracellular recording. The oscillation has a p...In the procerebrum (PC), the olfactory center, of the land slug Limax, an oscillation of local field potential (LFP) with 0.5 - 1 Hz is observed by electrophysiological extracellular recording. The oscillation has a phase delay along the distal-proximal axis, resulting in the propagation of waves from the distal to proximal region. One important advantage of nervous systems of mollusks such as Limax is that their nervous systems in vitro retain several types of computational properties found in vivo (e.g. learning and memory). A previous study showed that the LFP frequency in the PC of Limax increased specifically in response to innately aversive and in vitro aversively conditioned odors. In the present study, we examined spatiotemporal neural activity changes induced in the PC by those odors using the fluorescent voltage imaging technique. The results showed that innately aversive (onion and hexanol) and in vitro aversively conditioned (carrot, which is innately attractive) odors specifically induced an increase in propagation speed of the neural activity in the PC, while innately attractive odors did not induce it. The results also suggested that the avoidance behavior by those odors might be induced by the increase of propagation speed and the following increases in the discharges of the partial nerve that transmits the motor output.展开更多
目的探讨双源CT双能量成像技术对体外泌尿系结石成分分析的价值。材料与方法收集97例已知化学成分的泌尿系结石标本,结石成分包括草酸钙55例、羟基磷灰石13例、尿酸9例、胱氨酸4例、磷酸铵镁2例、混合结石14例,将结石编号后放入猪肾中...目的探讨双源CT双能量成像技术对体外泌尿系结石成分分析的价值。材料与方法收集97例已知化学成分的泌尿系结石标本,结石成分包括草酸钙55例、羟基磷灰石13例、尿酸9例、胱氨酸4例、磷酸铵镁2例、混合结石14例,将结石编号后放入猪肾中进行双源CT双能量扫描,测量80 k V及140 k V下结石CT值,计算CT值差值及双能量指数(DEI),分析结石成分,计算每种结石利用双能量技术分析结石成分的敏感度、特异度及准确度。结果双能量分析结石成分结果为草酸钙59例、羟基磷灰石11例、尿酸9例、胱氨酸4例,混合结石14例。2例羟基磷灰石、2例磷酸镁铵双能量分析均为草酸钙结石,准确率为95.88%(93/97)。不同成分结石双能量扫描(80 k V与140 k V)CT值差值及DEI大小依次为:草酸钙结石>羟基磷灰石>尿酸结石>胱氨酸结石(F=24.09、11.80,P<0.01);双能量扫描分析草酸钙、羟基磷灰石、尿酸、胱氨酸结石的敏感度分别为100.00%、84.60%、100.00%、100.00%,特异度分别为85.70%、100.00%、100.00%、100.00%,准确度分别为95.18%、97.59%、100.00%、100.00%。结论双源CT双能量成像技术可以准确分析体外泌尿系结石成分,对体内结石成分分析具有重要的临床价值。展开更多
文摘Magnetic Resonance imaging (MR imaging) as a powerful non-invasive modality is of high global interest for early cancer detection. The aim of this study was the synthesis of nanodendrimer and its conjugate with monoclonal antibody C595 against breast cancer cell, followed by its chelating with gadolinium for its magnetic property. First, anti-MUC-1 monoclonal antibody C595 was coupled to a biodegradable biocompatible Anionic Linear Globular Dendrimer G2 (having polyethylene glycol core and citric acid shell). Then prepared nanocomplex loaded by gadolinium to make novel agent of functional MR imaging. Anticancer effects and MR imaging parameters of the prepared nanoconjugate was investigated under in vitro conditions doing performing several studies such as evaluation of monoclonal antibody C595 binding to mucine-1 (MUC-1) cell, its purification, size of nanoconjugate and relaxivity measurements. The obtained data showed a powerful relaxations as well as selective MUC-1 antigen binding to the cell. Based on the findings from the present research Gd3+-ALGDG2-C595 nano-probe may be a potential breast molecular imaging and therapeutic agent. However, further investigations by in vivo studies and clinical trials are in the pipeline.
基金funded by Beijing Natural Science Foundation (Nos.L222109, 3222018)Military Health Care Project(No.22BJZ22)+6 种基金Science Foundation of China University of Petroleum (Nos.2462019QNXZ02, 2462019BJRC007)National Natural Science Foundation of China (Nos.52211530034, 82273236)Guangdong Provincial Basic and Applied Basic Research Foundation (Nos.2022A151522004, 2022A1515220042)Science and Technology Innovation Commission of Shenzhen (Nos.JSGG20210802153410031, JCYJ20220530141609021)Science and Technology Plan of Shenzhen Nanshan District (No.NS016)Discipline Leader Foundation of Huazhong University of Science and Technology Union Shenzhen Hospital (No.YN2021002)Crosswise Project of Daan Gene (No.HXKY2022002)。
文摘Liposomes are one of the significant classes of antitumor nanomaterials and the most successful nanomedicine drugs in clinical translation. However, it is difficult to accurately reveal liposome delivery modes and drug release rates at different p H values to assess the biodistribution and drug delivery pathways in vivo. Here, we established a strategy to integrate Bi-doped carbon quantum dots(CQDs)with liposomes to produce fluorescence visualization and therapeutic effects, namely lipo/Bi-doped CQDs.Lipo/Bi-doped CQDs show good water solubility and physicochemical properties, which can be used for in vitro labeling of colon cancer(CT26) cells and in vivo imaging localization tracking tumors for monitoring. Simultaneously, thanks to the excellent p H sensitivity and ion doping characteristic of Bi-doped CQDs, lipo/Bi-doped CQDs can be used to reveal the drug release rate of liposomes at different p H values and exhibit potential effects in vivo antitumor therapy.
文摘To investigate the validity and accuracy of tissue Doppler imaging (TDI) using a novel balloon phantom, validation of TDI myocardial velocity measurements has been carried out indirectly from conventional M mode images. However it is not a true and independent gold standard. We described a new TDI validation method by using a specially developed left ventricular balloon model mounted in a water bath and constructed using two pear shaped balloons. It was connected to a pulsatile flow pump at 8 stroke volumes (50-85 ml/beat). The displacement and velocity of the balloon walls were recorded simultaneously by video imaging and TDI on a GE Vingmed System Five with a 5 MHz phased array probe at the highest frame rates available. Conventional M mode and 2 D imaging verified that our balloon model mimicked the shape and wall motion of left ventricle. There was a good correlation and agreement between the maximum video excursion of the anterior and posterior walls of the phantom and the results of the temporal integration of digital distance data by TDI (Anterior wall: r =0.97, SEE=0.24 mm, ±s =0.04±0.24 mm; Posterior wall: r =0.95, SEE=0.22 mm, ±s =0.03±0.24 mm). Analysis of the velocity profile by the TDI method showed that the velocity at each measured point was correlated well with the velocity obtained from the video images (Anterior wall: r =0.97, SEE=0.30 mm, ±s =-0.04±0.28 mm; Posterior wall: r =0.97, SEE=0.30 mm, ±s =0.04±0.28 mm). Our balloon model provided a new independent method for the validation of TDI data. This study demonstrated that the present TDI system is reliable for measuring wall motion distance and velocity.
文摘A simple and rapid method was established to study vascular permeability by in vitroperfused endothelial cell monolayers cultured on micropore filter membrane.It can be used todetermine filtration coefficient （K<sub>f</sub>） to small molecules and osmotic reflection coefficient （σ） toproteins of the endothelial monolayer.Hanks’ balanced salt solution （HBSS） or 5g/L albuminin HBSS was used to perfuse the confluent endothelial monolayer at the hydrostatic pressure of2.45kPa （25cm H<sub>2</sub>O）.Control K<sub>f</sub> values were 10.1±0.75 and 3.6±0.75μl·min<sup>-1</sup>·cm<sup>-2</sup>·kPa（-1）（±,n=3） respectively for the perfusion of HBSS and albumin HBSS,suggesting that al-bumin may decrease endothelial monolayer permeability to water and small molecules.After ex-posure of endothelial monolayer to 10<sup>-8</sup>mol/L platelet-activating factor （PAF） for 30min,K<sub>f</sub>values increased to 193.1% and 133.3% respectively.Protein clearance rate (μl.min<sup>-1</sup>·cm<sup>-2</sup>)and osmotic reflection coefficient of the control endothelial monolayer were 8.0±3.22 and 0.37±0.09 respectively and those of the PAF treated endothelial monolayer 12.2±2.95μl·min<sup>-1</sup>·cm<sup>-2</sup> and 0.18±0.06,revealing increased permeability to albumin.Computer-assisted imageprocessing demonstrated that PAF treatment decreased cell area while increased cell form factorand intercellular space,suggesting that endothelial cells retracted and rounded,which may bean important mechanism of PAF-induced increase of vascular permeability.
文摘In the procerebrum (PC), the olfactory center, of the land slug Limax, an oscillation of local field potential (LFP) with 0.5 - 1 Hz is observed by electrophysiological extracellular recording. The oscillation has a phase delay along the distal-proximal axis, resulting in the propagation of waves from the distal to proximal region. One important advantage of nervous systems of mollusks such as Limax is that their nervous systems in vitro retain several types of computational properties found in vivo (e.g. learning and memory). A previous study showed that the LFP frequency in the PC of Limax increased specifically in response to innately aversive and in vitro aversively conditioned odors. In the present study, we examined spatiotemporal neural activity changes induced in the PC by those odors using the fluorescent voltage imaging technique. The results showed that innately aversive (onion and hexanol) and in vitro aversively conditioned (carrot, which is innately attractive) odors specifically induced an increase in propagation speed of the neural activity in the PC, while innately attractive odors did not induce it. The results also suggested that the avoidance behavior by those odors might be induced by the increase of propagation speed and the following increases in the discharges of the partial nerve that transmits the motor output.
文摘目的探讨双源CT双能量成像技术对体外泌尿系结石成分分析的价值。材料与方法收集97例已知化学成分的泌尿系结石标本,结石成分包括草酸钙55例、羟基磷灰石13例、尿酸9例、胱氨酸4例、磷酸铵镁2例、混合结石14例,将结石编号后放入猪肾中进行双源CT双能量扫描,测量80 k V及140 k V下结石CT值,计算CT值差值及双能量指数(DEI),分析结石成分,计算每种结石利用双能量技术分析结石成分的敏感度、特异度及准确度。结果双能量分析结石成分结果为草酸钙59例、羟基磷灰石11例、尿酸9例、胱氨酸4例,混合结石14例。2例羟基磷灰石、2例磷酸镁铵双能量分析均为草酸钙结石,准确率为95.88%(93/97)。不同成分结石双能量扫描(80 k V与140 k V)CT值差值及DEI大小依次为:草酸钙结石>羟基磷灰石>尿酸结石>胱氨酸结石(F=24.09、11.80,P<0.01);双能量扫描分析草酸钙、羟基磷灰石、尿酸、胱氨酸结石的敏感度分别为100.00%、84.60%、100.00%、100.00%,特异度分别为85.70%、100.00%、100.00%、100.00%,准确度分别为95.18%、97.59%、100.00%、100.00%。结论双源CT双能量成像技术可以准确分析体外泌尿系结石成分,对体内结石成分分析具有重要的临床价值。
