The research explored growth and development, economic profits, and eco- logical benefits of released black carps on basis of 21 group ecological data on 52 365 fish within 6 months. The results indicated that the age...The research explored growth and development, economic profits, and eco- logical benefits of released black carps on basis of 21 group ecological data on 52 365 fish within 6 months. The results indicated that the age and growth index of released black carp were normal and body length and size were coordinated; 88.89% released black carps exceeded theoretical weight, with an average of 25.15%, which indicated that black carps are hardly to reproduct naturally. In 2013, 310 000 black carps were released in the Reservoir, and 123 tons black carps were obtained, with direct economic value of 2.46 million yuan and indirect econom- ic value of 3.84 million yuan. It can be concluded based on averages that 123 tons black carps can be produced in 2013 and 3 690 kg nitrogen and 740 kg phospho- rus can be removed. The releasing of fish has shown much more economic, ecological and social benefits in Yantan Reservoir, but the hidden dangers still exist on fishery environment. Therefore, it is necessary to further increase the number of re- leased black carps in the Reservoir.展开更多
A series of experiments based on electrophoretical and biochemical assays were conducted to partially characterize proteinases present in the hepatopancreas and intestine of black carp （Mylopharyngodon piceus）, a...A series of experiments based on electrophoretical and biochemical assays were conducted to partially characterize proteinases present in the hepatopancreas and intestine of black carp （Mylopharyngodon piceus）, and investigate enzymatic activity and protein digestibility in vitro. Casein digestion assays revealed the presence of acidic proteinases with optimum activity in the range of pH 2.0-2.5 and alkaline proteinases with significantly higher activities both in the range of pH 8.1-8.6 and near pH 9.5. The inhibition and substrate specificity assays showed that trypsin and chymotrypsin are the main active components of the alkaline proteinases. The SDS-substrate-PAGE showed that the crude extract of black carp intestine had eight types of alkaline proteinases with the molecular mass range of 27.5-78.5 kDa while the crude extract of black carp hepatopancreas had six types of alkaline proteinases with the molecular mass range of 27.5-78.5 kDa. These enzymes were characterized as trypsin （27.5 kDa, 30.1 kDa）, chymotrypsin （40.5 kDa, 42.5 kDa）, serine proteinases （32.1 kDa, 33.2 kDa） and non-serine proteinase （61.5 kDa, 78.5 kDa）.In vitro protein digestibility assays showed that black cardcan be able to utilize a wider range of proteins.展开更多
To make more effective use of underutilized resources, pepsin-solubilized collagen (PSC) was successfully extracted from the skin of black carp (Mylopharyngdon piceus) with a yield of 45.7% based on dry weight. The PS...To make more effective use of underutilized resources, pepsin-solubilized collagen (PSC) was successfully extracted from the skin of black carp (Mylopharyngdon piceus) with a yield of 45.7% based on dry weight. The PSC comprising two identical α1-chains and one α2-chain with no disulfide bond was characterized as type I, and it contained 195 imino acid residues vs. 1000 amino acid residues. The collagen showed an absorption edge around 218 nm, which was lower than the maximum absorption wavelength of other PSC. The denaturation temperature of PSC was 25.6°C, which was lower than that of porcine collagen by approximately 11°C. The isoelectric point (pI) was estimated to be 8.23, and the collagen was soluble at an acidic pH as well as below 40 g/L NaCl. It is thought that the high yield and stability of PSC from the skin of black carp warrant its application as a new source of collagen for industrial purposes.展开更多
Ecological safety is a major consideration in the commercialization of transgenic fish. Development of sterile transgenic triploid fish through hybridization of transgenic tetraploid fish and transgenic diploid fish i...Ecological safety is a major consideration in the commercialization of transgenic fish. Development of sterile transgenic triploid fish through hybridization of transgenic tetraploid fish and transgenic diploid fish is a feasible way to solve this problem. The "all-fish" transgene, pbcAbcGHc, containing the black carp β-actin gene promoter and the open reading frame (ORF) of the black carp growth hormone (GH) gene was constructed and introduced into fertilized eggs of allotetraploid fish through microinjection. Contrast cultivation results showed that the growth rate of 150 day-old P 0 black carp GH gene transgenic allotetraploid fish was much higher than that of controls. Sixty 150 day-old transgenic allotetraploid fish were assayed by PCR for transgene integration and 90% of fish were positive for the transgene. The transgene was detected in 13 of 20 sperm samples from male transgenic allotetraploid fish. RT-PCR detected transcription of the exogenous black carp GH gene in the muscle, liver, kidney and ovaries of the largest transgenic allotetraploid fish. This study has developed P 0 black carp GH gene transgenic allotetraploid fish with a highly increased growth rate, which provides a solid foundation for the establishment of a pure line of transgenic allotetraploid fish and for the large scale production of sterile transgenic triploid fish.展开更多
基金Supported by Guangxi Natural Science Foundation(2013GXNSFEA053003)Special Fund for Agro-scientific Research in the Public Interest(201303048)Germplasm Resource Protection Project of Ministry of Agriculture([2013]75)~~
文摘The research explored growth and development, economic profits, and eco- logical benefits of released black carps on basis of 21 group ecological data on 52 365 fish within 6 months. The results indicated that the age and growth index of released black carp were normal and body length and size were coordinated; 88.89% released black carps exceeded theoretical weight, with an average of 25.15%, which indicated that black carps are hardly to reproduct naturally. In 2013, 310 000 black carps were released in the Reservoir, and 123 tons black carps were obtained, with direct economic value of 2.46 million yuan and indirect econom- ic value of 3.84 million yuan. It can be concluded based on averages that 123 tons black carps can be produced in 2013 and 3 690 kg nitrogen and 740 kg phospho- rus can be removed. The releasing of fish has shown much more economic, ecological and social benefits in Yantan Reservoir, but the hidden dangers still exist on fishery environment. Therefore, it is necessary to further increase the number of re- leased black carps in the Reservoir.
文摘A series of experiments based on electrophoretical and biochemical assays were conducted to partially characterize proteinases present in the hepatopancreas and intestine of black carp （Mylopharyngodon piceus）, and investigate enzymatic activity and protein digestibility in vitro. Casein digestion assays revealed the presence of acidic proteinases with optimum activity in the range of pH 2.0-2.5 and alkaline proteinases with significantly higher activities both in the range of pH 8.1-8.6 and near pH 9.5. The inhibition and substrate specificity assays showed that trypsin and chymotrypsin are the main active components of the alkaline proteinases. The SDS-substrate-PAGE showed that the crude extract of black carp intestine had eight types of alkaline proteinases with the molecular mass range of 27.5-78.5 kDa while the crude extract of black carp hepatopancreas had six types of alkaline proteinases with the molecular mass range of 27.5-78.5 kDa. These enzymes were characterized as trypsin （27.5 kDa, 30.1 kDa）, chymotrypsin （40.5 kDa, 42.5 kDa）, serine proteinases （32.1 kDa, 33.2 kDa） and non-serine proteinase （61.5 kDa, 78.5 kDa）.In vitro protein digestibility assays showed that black cardcan be able to utilize a wider range of proteins.
文摘To make more effective use of underutilized resources, pepsin-solubilized collagen (PSC) was successfully extracted from the skin of black carp (Mylopharyngdon piceus) with a yield of 45.7% based on dry weight. The PSC comprising two identical α1-chains and one α2-chain with no disulfide bond was characterized as type I, and it contained 195 imino acid residues vs. 1000 amino acid residues. The collagen showed an absorption edge around 218 nm, which was lower than the maximum absorption wavelength of other PSC. The denaturation temperature of PSC was 25.6°C, which was lower than that of porcine collagen by approximately 11°C. The isoelectric point (pI) was estimated to be 8.23, and the collagen was soluble at an acidic pH as well as below 40 g/L NaCl. It is thought that the high yield and stability of PSC from the skin of black carp warrant its application as a new source of collagen for industrial purposes.
基金supported by the National Special Fund for Scientific Research in Public Benefits (Grant No. 20090304608)the National Basic Research Program of China (Grant No. 2007CB109006)+1 种基金the National Natural Science Foundation of China (Grant No. 30500294)the Excellent Talent Program of Hunan Normal University (Grant No. ET31004)
文摘Ecological safety is a major consideration in the commercialization of transgenic fish. Development of sterile transgenic triploid fish through hybridization of transgenic tetraploid fish and transgenic diploid fish is a feasible way to solve this problem. The "all-fish" transgene, pbcAbcGHc, containing the black carp β-actin gene promoter and the open reading frame (ORF) of the black carp growth hormone (GH) gene was constructed and introduced into fertilized eggs of allotetraploid fish through microinjection. Contrast cultivation results showed that the growth rate of 150 day-old P 0 black carp GH gene transgenic allotetraploid fish was much higher than that of controls. Sixty 150 day-old transgenic allotetraploid fish were assayed by PCR for transgene integration and 90% of fish were positive for the transgene. The transgene was detected in 13 of 20 sperm samples from male transgenic allotetraploid fish. RT-PCR detected transcription of the exogenous black carp GH gene in the muscle, liver, kidney and ovaries of the largest transgenic allotetraploid fish. This study has developed P 0 black carp GH gene transgenic allotetraploid fish with a highly increased growth rate, which provides a solid foundation for the establishment of a pure line of transgenic allotetraploid fish and for the large scale production of sterile transgenic triploid fish.
基金This work was supported by the National 973 Program (No.2001CB109006), the Scientific Funds of the Educational Bureau of Hunan Province (No.04C378), and the Ph.D Programs Foundation in Hunan Normal University (No. 24030610)
