Relationship Between Water-Stable Aggregates and Nutrients in Black Soils After Reclamation

Water-stable aggregates, which are an index for the evaluation of the structural properties of the soil, are affected by many factors. Zhaoguang Farm, Longzhen Farm, and Jiusan Farm were chosen as the representative study sites in the region of black soils, a typical soil resource in Northeast China. The variation in the content of 〉 0.25 mm water-stable aggregates and its relationship with the nutrients in black soil were investigated after different years of reclamation. The results showed that the 〉 0.25 mm water-stable aggregates were more in the surface than in the subsurface soil and they changed in the following order： Longzhen Farm 〉 Zhaoguang Farm 〉 Jiusan Farm. The water-stable aggregates decreased sharply at the initial stage of reclamation and then became stable gradually with time. They were significantly correlated with the contents of organic C, total N, total P, and CEC in black soil, with the correlation coefficients r being 0.76, 0.68, 0.61, and 0.81 （P 〈 0.01）, respectively; however, their relationships with available P, available K, and total K were unclear. These showed that organic matter was the cementation of soil water-stable aggregates. Increasing decompositions and decreasing inputs of organic matter after reclamation were responsible for the amount of reduction of the water-stable aggregates. Thus, to maintain good soil aggregate structure, attention should be paid to improvement of soil nutrient status, especially the supply of organic C and N.

Light Fraction Carbon and Water-Stable Aggregates in Black Soils

The distribution of light fraction carbon （LF-C） in the various size classes of aggregates and its relationship to water- stable aggregates as well as the influence of cultivation on the organic components in virgin and cultivated black soils were studied by wet sieving and density separation methods. The total organic carbon （TOC） and LF-C were significantly higher （P≤ 0.05） in the virgin soils than in the cultivated soils. The LF-C in aggregates of different size classes varied from 0.9 to 2.5 g kg^-1 in the cultivated soils and from 2.5 to 7.1 g kg^-1 in the virgin soils, whereas the ratio of LF-C to TOC varied from 1.9% to 7.3% and from 5.0% to 12.2%, respectively. After being incubated under constant temperature and controlled humidity for three months, the contribution of LF-C to TOC sharply decreased to an amount （1.7%4.5%） close to the level in soils that had been cultivated for 20 to 25 years （1.3%-8.8%）. As a result, the larger water-stable macro-aggregates （especially 〉 1 mm） decreased sharply, indicating that the LF-C pool in virgin soils declined quickly after cultivation, which reduced the water stability of soil aggregates.

Effects of Acetochlor and Methamidophos on Fungal Communities in Black Soils

Using plate counting and ergosterol assay, single and joint effects of acetochlor and methamidophos on the dynamicsof soil fungal population and total fungal biomass in the black soil zone of Northeast China were investigated. The resultsdemonstrated that acetochlor at high concentration levels (150 and 250 mg kg-1) had an acute and mostly chronic toxicityon both the soil fungal population and total fungal biomass, but at a low concentration (50 mg kg-1) generally had astimulating effect that was stronger with total fungal biomass than with the soil fungal population. Methamidophos ata high concentration level (250 mg kg-1) alone and almost all of its combinations with various dosages of acetochlorincreased the soil fungal population, whereas at most sampling dates with 250 mg methamidophos kg-1 soil, total fungalbiomass increased, but in combination with acetochlor it was decreased in the early period of incubation and then increased28 days after incubation. Thus, through measuring the number of colony forming unit of the soil fungal population alongwith the total fungal biomass, a better understanding on effects of agrochemicals on soil fungi could be made.

Spatio-temporal Evaluation of Multi-scale Cultivated Land System Resilience in Black Soil Region from 2000 to 2019:A Case Study of Liaoning Province,Northeast China

It is of great significance to systematically analyze the cultivated land system resilience(CLSR) for the black soil protection and national food security.The CLSR is impacted by planting structure adjustment and cultivated land quality decline,posing major hidden dangers to food security.It is urgent to evaluate the CLSR at multiple spatio-temporal scales.This study took Liaoning Province in the black soil region of Northeast China as an example.Based on the resilience theory,this study constructed the CLSR evaluation system from the input-feedback perspective at the provincial-scale and the city-scale,and used the rank-sum ratio comprehensive evaluation method(RSR) to analyze the key influencing factors of CLSR in Liaoning Province and its 14 cities from 2000 to 2019.The results showed that:1) the time series changes of CLSR at the provincial-scale and the city-scale in Liaoning Province were similar,both showing an increasing trend.2) The CLSR in Liaoning Province presented a spatial pattern of ‘high in the west and low in the east’ at the city-scale.3) There were seven and six main influencing factors of CLSR at the provincial-scale and the city-scale,respectively.In addition to the net income per capita of rural households,other influencing factors of CLSR were different at the provincial-scale and the city-scale.The feedback factors were dominant at the provincial-scale,and the input factors and feedback factors were dominant at the city-scale.The results could provide a reference for the utilization of black soil and draw on the experience of regional agricultural planning and adjustment.

Effects of Slow-release Nitrogen Fertilizer on Yield and Nitrogen Accumulation of Summer Maize in Shajiang Black Soil Area

[Objectives] This study was conducted to verify the field application effect of slow-release nitrogen fertilizer on summer maize in Shajiang black soil area by simultaneous sowing and fertilization, and explore the application scope and nitrogen metabolism mechanism, so as to lay a foundation for fertilizer reduction and efficiency improvement. [Methods] With maize variety Beiqing 340 and sulfur-coated urea as experimental materials, five nitrogen application levels were set, namely, control (C0), slow-release nitrogen 70 kg/hm^(2) (C70), slow-release nitrogen 140 kg/hm^(2) (C140), slow-release nitrogen 210 kg/hm^(2) (C210) and slow-release nitrogen 280 kg/hm^(2) (C280). The phosphorus and potassium fertilizers were all in accordance with the unified standard. [Results] With the application rate of slow-release nitrogen increasing, the nitrogen accumulation in organs increased first and then decreased after tasseling stage of maize. In order to reduce the fertilizing amount and increase efficiency, 210 kg/hm^(2) of slow-release nitrogen fertilizer was the best fertilizing amount for summer maize in Shajiang black soil area. [Conclusions] This study provides reference for fertilizer reduction, efficiency improvement and sustainable development of summer maize in Shajiang black soil area.

Trade-off and Synergy of Rural Functions Under County Depopulation in the Typical Black Soil Region of Northeast China

As the population continues to shrink in the black soil region of Northeast China since 2000,it is critical to master the impact of population shrinkage on rural functions to realize rural revitalization and sustainable development.In this study,we focused on the impacts of depopulation on the evolution and interrelationship of rural subfunctions.Based on the rural function indexes system,the TOPSIS(Technique for Order Preference by Similarity to an Ideal Solution)method,spatial analysis method,and mathematical statistics analysis method were used to summarize the spatial and temporal characteristics of rural function development,as well as the effect of population shrinkage in the typical black soil region of Northeast China.The results showed that depopulation varied in the extent and duration between the forested region and plain areas,which both impacted the trajectories of rural subfunctions evolution.For the economic development function and ecological conservation function,the effect of continuous slight depopulation was beneficial,while the effect of rapid depopulation was adverse,which was exactly opposite to the agricultural production function.All forms of population shrinkage were conducive to the development of the social security function.With the deepening population shrinkage,depopulation mainly promoted the collaborative development between subfunctions in this study,except the relationship between agricultural production and social security function.But effects of depopulation on the interrelationship of rural subfunctions varied between the forested region and plain areas in some cases.The results provided evidence for the cognition that population shrinkage had complicated effects on rural subfunctions.

Evaluation and Driving Force Analysis of Cultivated Land Quality in Black Soil Region of Northeast China

Cultivated land is an important natural resource to ensure food,ecological and economic security.The cultivated land quality evaluation(CQE)is greatly significant for protecting and managing cultivated land.In this study,320 counties in the black soil region of Northeast China(BSRNC)represent the research units used to construct the CQE system measuring the soil properties(SP),cultivated land productivity(CLP),ecological environment(EE)and social economy(SE).The total of 19 factors were selected to calculate the integrated fertility index(IFI)and divided into grades.Simultaneously,we used the coupling coordination degree model to comprehensively analyze the spatial pattern of the cultivated land quality(CLQ)in the BSRNC,and use the structural equation model(SEM)to analyze the driving mechanism.The results show the following:1)The CLQ of 262 counties in the BSRNC is in a state of coupling and coordination,and the coupling and coordination degree presents a spatial distribution pattern of‘high in the southwest and northeast,low in the northwest and southeast’.The coordinated development degree of 271 counties is between 0.4 and 0.6,which is in a transitional state between coordination and disorder.2)The CLQ in the BSRNC is generally good,with an average grade of 3.High-quality cultivated land accounts for 58.45%of all counties,middle-and upper-quality cultivated land accounts for 27.05%,and poor-quality cultivated land accounts for 14.49%.3)The SEM analysis shows that the SP,CLP,EE,and SE all influence the CLQ.Among them,the SP has the largest driving force on the CLQ,while the SE has the smallest driving force on it.The results confirm that the main factors affecting the evaluation results are crop productivity level,normalized difference vegetation index,ratio vegetation index,difference vegetation index,and organic carbon content.When implementing protection measures in counties with a low CLQ,considering a balanced coordination of multiple systems and reasonably controlling the quality degradation are important.This study provides the current situation and driving factors of the CLQ in the BSRNC and will play an important role in black soil governance and utilization.

Changes of spring wind erosion based on wind erosion climate factor in the black soil region of Northeast China

The dry and windy climate and low ground cover in spring in the black soil region of Northeast China make the soil strongly affected by wind erosion,which seriously threatens the food security and ecological security of this region.In this paper,based on the daily observation data of 124 meteorological stations in study area from 1961 to 2020,seasonal and monthly wind erosion climate factor(C)in spring(March to May)were calculated by using the method proposed by the Food and Agriculture Organization of the United Nations(FAO),the wind erosion characterization in spring were systematically analyzed based on C by various statistical analysis methods.The results showed that in the past 60 years,spring wind erosion climate factor(CSp)and monthly C of the whole region and each province(region)all showed highly significant decreasing trend,but they began to show rebounded trend in the middle or late 2000s.CSp of the study area showed a significant upward trend since 2008 with an increase of 4.59(10a)^(-1).The main contributors to this upward trend are the changes of C in March and in April.For the four provinces(regions),CSp in Heilongjiang,Jilin,Liaoning and eastern Inner Mongolia all showed rebounded since 2008,2011,2008 and 2009,respectively.The rebounded trend of CSp in eastern Inner Mongolia was the most obvious with a tendency rate of 11.27(10a)^(-1),and its mutation occurred after 1984.The rebound trend of CSp in Heilongjiang Province takes the second place,with a trend rate of 4.72(10a)^(-1),but there’s no obvious time mutation characteristics.The spatial characteristics of CSpand monthly C are similar,showing decreasing characteristics centered on the typical black soil belt of Northeast China.Compared with 1961-1990,in the period from 1991 to 2020,the proportion of high value areas(CSp>35,monthly C>10)has decreased to varying degrees,while the proportion of low value areas(CSp≤10,monthly C≤4)has increased.The trends of seasonal and monthly C in 82.2%~87.7%of the stations show significant decreases at 95%confidence level.CSp is closely related to wind speed at 2m height,temperature difference,minimum temperature and precipitation in the same period,of which the correlation between CSp and wind speed is the strongest,indicating that the main control factor for CSp in the study area is wind speed,but the impact of the change of temperature and precipitation on CSp cannot be ignored.

Change of cultivated land area and effect on ecosystem service in black soil region in Northeast China: a case study of Lishu County, Jilin Province

It is of great significance to study the influence of spatial pattern change of cultivated land on eco-system service,including the sustainable utilization and ecological protection in black soil region in Northeast China.Taking Lishu County of Jilin Province as the study area,we quantitatively evaluated soil conservation,habitat quality,carbon storage and grain production service,and analyzed the change of cultivated land and ecosystem service pattern in Lishu County from 1990 to 2020 by using Pearson correlation analysis on the correlation between cultivated land change and ecosystem service.The results show that:(1)The cultivated land area of Lishu County was reduced by 19.67 km2,the characteristics of cultivated land change are signifi-cant;(2)the overall change rate of carbon storage,habitat quality,soil conservation and grain production ser-vice in the study area was 10.82%,-0.09%,4.07%and 1.80%,respectively.They all had significant spatial differentiation features;(3)the change of habitat quality,grain production service showed a significant pos-itive correlation with the change of cultivated land area.The change in soil conservation and carbon storage were negatively related to cultivated land area.In order to promote the rational development and utilization of cultivated land in the research area,it is suggested to scientifically carry out the comprehensive utilization of cultivated land reserve resources such as saline-alkali land,strengthen the management and protection of newly added cultivated land at the same time.The results can provide scientific reference for formulating comprehensive land use planning under ecological security conditions.

Soil structure characters of different soil and water conservation plantations in typical black soil region

A study was conducted to determine the characters of soil structure in different water and soil conservation forests in Keshan County,northwest of Heilongjiang Province,China.The soil bulk density,the ratio of non-capillary porosity and capillary porosity（NCP/CP）,and the generalized soil structure index（GSSI） were measured for Fraxinus mandshurica,Larix gmelini,Pinus sylvestris var.mongolica,and Picea koraiensis plantations as well as the abandoned land（as control） adjacent to the forests in typical black soil region.Results show that at soil depth of 0–30cm,the soil bulk density of F.mandshurica forest and L.gmelini forest was lower than that of P.sylvestris var.mongolica forest and P.koraiensis forest,with the relative decrease of 8.04%–11.01%.The soil bulk density of L.gmelini forest was significantly different from that of the P.sylvestris var.mongolica forest and P.koraiensis forest.The NCP/CP values of the four types of plantations were all higher（59.75%–128.82% relatively） than that of abandoned land（p〈0.05）,indicating that the soil aeration and permeability under forest were enhanced,especially under L.gmelini forest.GSSI values of the four types of forests were also relatively higher（2.98%–4.36%） than abandoned land（p〈0.05）,indicating that those soil and water conservation forests,especially the F.mandshurica forest and P.koraiensis forest,can promote soil condition to approximate ideal soil structure.The result of this study can provide theoretical basis for scientifically evaluating the effects of vegetation restoration on soil quality in typical black soil region.

Study on Food-making Quality of Strong-gluten Wheat Varieties from Lime Concretion Black Soil Area in the Huanghuai Wheat Region

In this study, 13 strong-gluten wheat varieties screened by the Key Project of Modern Agricultural Industry Technology System ＂Study on Industrial Technology for Strong-gluten Wheat from Lime Concretion Black Soil Area in the Huanghuai Wheat Region＂ were used as experimental materials to investigate their bread-making quality, noodle-making quality and other related characteristics. The results showed that more than half of the wheat varieties had better bread-making quality; the bread made from wheat with longer dough mixing time than 3.0 min had better texture, lighter color, and better taste. All these 13 strong-gluten wheat varieties showed good noodle-making quality in color, appearance, smoothness and taste; the differences between varieties were mainly found in palatability and viscoelasticity. Jimai 20, Xinong 979, Zhengmai 7698, Ji＇nan 17 and Zhengmai 9023 exhibited excellent bread-making quality; Zhengmai 366, Jimai 20 and Xinong 979 displayed excellent noodle-making quality. Fresh dough sheets made from Zhengmai 366, Jimai 20 and Xinong 979 exhibited slight color variation within 24 h and high peak starch paste viscosity; dry and cooked noodles made from Zhengmai 366, Jimai 20 and Xinong 979 had good quality.

Straw mulching alters the composition and loss of dissolved organic matter in farmland surface runoff by inhibiting the fragmentation of soil small macroaggregates

Straw mulching is a widespread practice for reducing the soil carbon loss caused by erosion.However,the effects of straw mulching on dissolved organic matter(DOM)runoff loss from black soil are not well studied.How straw mulching affects the composition and loss of runoff DOM by changing soil aggregates remains largely unclear.Here,a straw mulching treatment was compared to a no mulching treatment(as a control)on sloping farmland with black soil erosion in Northeast China.We divided the soil into large macroaggregates(>2 mm),small macroaggregates(0.25-2 mm),and microaggregates(<0.25 mm).After five rain events,the effects of straw mulching on the concentration(characterized by dissolved organic carbon(DoC)and composition(analyzed by fluorescence spectroscopy)of runoff and soil aggregate DOM were studied.The results showed that straw mulching reduced the runoff amount by 54.7%.Therefore,although straw mulching increased the average DOc concentration in runoff,it reduced the total runoff DOM loss by 48.3%.The composition of runoff DOM is similar to that of soil,as both contain humic-like acid and protein-like components.With straw mulching treatment,the protein-like components in small macroaggregates accumulated and the protein-like components in runoff declined with rain events.Fluorescence spectroscopy technology may help in understanding the hydrological paths of rain events by capturing the dynamic changes of runoff and soil DOM characteristics.A variation partitioning analysis(VPA)indicated that the DOM concentration and composition of microaggregates explained 68.2%of the change in runoff DOM from no mulching plots,while the change in runoff DOM from straw mulching plots was dominated by small macroaggregates at a rate of 55.1%.Taken together,our results demonstrated that straw mulching reduces the fragmentation of small macroaggregates and the loss of microaggregates,thus effecting DOM compositions in soil and reducing the DOM loss in runoff.These results provide a theoretical basis for reducing carbon loss in sloping farmland.

Effect of Measurement Time on Emission Flux of CO_2 and N_2O in Black Soil Region

[Objective] The aim was to investigate and reveal effect of measurement time on emission flux of CO2 and N2O to ensure the optimum time of emission flux, in order to provide scientific reference for emission reduction of greenhouse gas in black soil region. [Method] Based on experiment of long-term fertilizer location in black soil region, the paper studied on daily dynamic variation of CO2 and N2O discharge in 3 key growth periods （booting stage, grain-filling stage and mature stage） to reveal differences of CO2 and N2O emission flux in different times. [Result] Daily variations of CO2 and N2O emission flux were large, from 205 mg/（m2·h） to 552 mg/（m2·h） for CO2 and from 51 h to 295 μg/（m2·h） for N2O. Trend of CO2 discharge in different growth times showed a unimodal curve, and the peak was at noon of 12：00 and the peak valley was at 3：00 am; discharge of N2O was small in day time at booting stage and large at night. Regardless of rice growth period effect on CO2 and N2O emission flux, representative time of CO2 discharge was 6：00-8：00 or 16：00-21：00; and time of N2O was 8：00-10：00 or 16：00-21：00; if CO2 and N2O emission fluxes were measured simultaneously, the optimum time was 16：00-18：00; if the measurement was started during 9：00-12：00, correction coefficients of CO2 and N2O were 0.81 and 0.90, respectively. [Conclusion] The result provided scientific reference for reduction of greenhouse gas emission in black soil region.

Study on Relative Soil and Water Conservation Benefits of Ridge Tillage in Different Terrain Conditions in the Black Soil Area of Northeast China

Ridge tillage, which is a very common and important tillage measure in the black soil area of northeast China, has some soil and water conservation bene- fits, but has little attention. It is very important to explore the spatial distribution of the ridge direction of the arable land and its soil and water conservation benefits in different terrain conditions in the black soil area. So Binxian County of Heilongjiang Province was selected as the study area, and 168 field investigation units were ex- tracted by stratified sampling method and investigated. According to equations of slope gradient factor and slope gradient in ridge direction, and based on the soft- ware of Arcmap, SPSS and Excel, the investigation data of soil and water loss in Binxian County were analyzed and counted, The results show that in plain, hilly and mountainous areas, the average ground slope gradients are 1.92°, 6.20° and 8.27° respectively, and the average slope gradients along ridge direction are 1.33°, 4.52°and 6.45° respectively, which account for about 70%, 73% and 78% of the average ground slope gradients in the same terrain condition; the relative quantities of soil erosion in the present ridge tillage condition account for about 55%, 69% and 67% respectively of that in down-slope ridge tillage conditions, so the present ridge tillage has obviously relative soil and water conservation benefits. Based on these results, the reasons of the present ridge tillage status were analyzed, and some reform measures were proposed. The results could not only help to comprehend the spatial distribution and soil and water conservation benefits of ridge tillage in the black soil area of Northeast China, but also provide scientific references for the layout of local soil and water conservation measures.

Spatial Variability of Nutrient Properties in Black Soil of Northeast China

A total of 1400 soil samples from the plow layer （0-20 cm） at an approximate interval of 5 km were collected in the autumn of 2002 over the entire black soil arable crops region to determine the spatial variability of seven variables, such as total organic matter content （OMC）, total N, total P, total K, alkali-dissolvable N （AN）, available P （AP） and available K （AK）, with classical statistics and geostatistical analysis across the entire black soil area in Northeast China. In nonsampled areas ordinary kriging was utilized for interpolation of estimated nutrient determinations. Classical statistics revealed highly significant （P ≤ 0.01） correlations with all seven of the soil properties, except for OMC with AP and total K with AK. In addition, using coefficients of variation, all soil properties, except for total K, were moderately variable. A geostatistical analysis indicated that structural factors, such as parent material, terrain, and water table, were the main causes of the spatial correlations. Strong spatial correlations were noted with OMC, total N, total P, AN, and AP, while they were moderate for total K and AK. The effective spatial autocorrelation of OMC, total N, total P, and AN ranged from 1 037 to 1353 km, whereas the ranges of total K, AP, and AK were only from 6 to 138 km. The fit of the experimental scmi-variograms to the theoretical models indicated that except for AN, kriging could successfully interpolate other six variables. Thns, the geostatistical method used on a large scale could accurately evaluate the spatial variability of most black soil nutrient properties in Northeast China.

Bacterial Community Structure and Diversity in a Black Soil as Affected by Long-Term Fertilization

Black soil （Mollisol） is one of the main soil types in northeastern China. Biolog and polymerase chain reactiondenaturing gradient gel electrophoresis （PCR-DGGE） methods were used to examine the influence of various fertilizer combinations on the structure and function of the bacterial community in a black soil collected from Harbin, Heilongjiang Province. Biolog results showed that substrate richness and catabolic diversity of the soil bacterial community were the greatest in the chemical fertilizer and chemical fertilizer＋manure treatments. The metabolic ability of the bacterial community in the manure treatment was similar to the control. DGGE fingerprinting indicated similarity in the distribution of most 16S rDNA bands among all treatments, suggesting that microorganisms with those bands were stable and not influenced by fertilization. However, chemical fertilizer increased the diversity of soil bacterial community. Principal com- ponent analysis of Biolog and DGGE data revealed that the structure and function of the bacterial community were similar in the control and manure treatments, suggesting that the application of manure increased the soil microbial population, but had no effect on the bacterial community structure. Catabolic function was similar in the chemical fertilizer and chemical fertilizer＋manure treatments, but the composition structure of the soil microbes differed between them. The use of chemical fertilizers could result in a decline in the catabolic activity of fast-growing or eutrophic bacteria.

Using ^(137)Cs Tracer Technique to Evaluate Erosion and Deposition of Black Soil in Northeast China

Soil and water losses through erosion have been serious in the black soil region of Northeast China. Therefore, a sloping cultivated land in Songnen Plain was selected as a case study to： 1） determine the ^137Cs reference inventory in the study area; 2） calculate erosion and deposition rates of black soil on different slope locations; 3） conduct a sensitivity analysis of some model parameters; and 4） compare overall outputs using four different models. Three transects were set in the field with five slope locations for each transect, including summit, shoulder-slope, back-slope, foot-slope, and toe-slope. Field measurements and model simulation were used to estimate a bomb-derived ^137Cs reference inventory in the study area. Soil erosion and deposition rates were estimated using four ^137Cs models and percentage of ^137Cs loss/gain. The ^137Cs reference value in the study area was 2 232.8 Bq m^-2 with ^137Cs showing a clear topographic pattern, decreasing from the summit to shoulder-slope, then increasing again at the foot-slope and reaching a maximum at the toe-slope, Predicted soil redistribution rates for different slope locations varied. Among models, the Yang Model （YANG-M） overestimated erosion loss but underestimated deposition. However, the standard mass balance model （MBM1） gave predictions similar to a mass balance model incorporating soil movement by tillage （MBM2）. Sensitivity analysis of the proportion factor and distribution pattern of ^137Cs in the surface layer demonstrated the impact of ^137Cs enrichment on calculation of the soil erosion rate. Factors influencing the redistribution of fallout ^137Cs in landscape should be fully considered as calculating soil redistribution rate using ^137Cs technique.

Effect of Long-Term Application of Chemical Fertilizers on Microbial Biomass and Functional Diversity of a Black Soil

An experiment with seven N, P, K-fertilizer treatments, i.e., control (no fertilizer), NP, NK, PK, NPK, NP2K, and NPK2 where P2 and K2 indicate double amounts of P and K fertilizers respectively, was conducted to examine the effect of long-term continuous application of chemical fertilizers on microbial biomass and functional diversity of a black soil (Udoll in the USDA Soil Taxonomy) in Northeast China. The soil microbial biomass C ranged between 94 and 145 mg kg-1, with the NK treatment showing a lower biomass; the functional diversity of soil microbial community ranged from 4.13 to 4.25, with an increasing tendency from control to double-fertilizer treatments, and to triple-fertilizer treatments. The soil microbial biomass, and the microbial functional diversity and evenness did not show any significant differences among the different fertilizer treatments including control, suggesting that the long-term application of chemical fertilization would not result in significant changes in the microbial characteristics of the black soil.

Basic Soil Productivity of Spring Maize in Black Soil Under Long-Term Fertilization Based on DSSAT Model

Increasing basic farmland soil productivity has significance in reducing fertilizer application and maintaining high yield of crops. In this study, we defined that the basic soil productivity （BSP） is the production capacity of a farmland soil with its own physical and chemical properties for a specific crop season under local environment and field management. Based on 22-yr （1990-2011） long-term experimental data on black soil （Typic hapludoll） in Gongzhuling, Jilin Province, Northeast China, the decision support system for an agro-technology transfer （DSSAT）-CERES-Maize model was applied to simulate the yield by BSP of spring maize （Zea mays L.） to examine the effects of long-term fertilization on changes of BSP and explore the mechanisms of BSP increasing. Five treatments were examined： （1） no-fertilization control （control）; （2） chemical nitrogen, phosphorus, and potassium （NPK）; （3） NPK plus farmyard manure （NPKM）; （4） 1.5 time of NPKM （1.5NPKM） and （5） NPK plus straw （NPKS）. Results showed that after 22-yr fertilization, the yield by BSP of spring maize significantly increased 78.0, 101.2, and 69.4% under the NPKM, 1.5NPKM and NPKS, respectively, compared to the initial value （in 1992）, but not significant under NPK （26.9% increase） and the control （8.9% decrease）. The contribution percentage of BSP showed a significant rising trend （P〈0.05） under 1.5NPKM. The average contribution percentage of BSP among fertilizations ranged from 74.4 to 84.7%, and ranked as 1.5NPKM〉NPKM〉NPK〉NPKS, indicating that organic manure combined with chemical fertilizers （I.5NPKM and NPKM） could more effectively increase BSP compared with the inorganic fertilizer application alone （NPK） in the black soil. This study showed that soil organic matter （SOM） was the key factor among various fertility factors that could affect BSP in the black soil, and total N, total P and/or available P also played important role in BSP increasing. Compared with the chemical fertilization, a balanced chemical plus manure or straw fertilization （NPKM or NPKS） not only increased the concentrations of soil nutrient, but also improved the soil physical properties, and structure and diversity of soil microbial population, resulting in an iincrease of BSP. We recommend that a balanced chemical plus manure or straw fertilization （NPKM or NPKS） should be the fertilization practices to enhance spring maize yield and improve BSP in the black soil of Northeast China.

Impact of Long-Term Fertilization on Phosphorus Status in Black Soil

A twelve-year field trial was carried out to determine the impact of long-term fertilization on phosphorus (P) distribu- tion in the soil profile, P balance, and the transformation and availability of soil P in a black soil (Phaeozem, FAO). Under a wheat-soybean-corn rotation, during twelve years where no fertilizer was applied, crops removed 60 and 81 mg P kg-1 soil in the control and NK treatment, respectively. About one third of the P absorbed by crops was originated from organic P. Ca2-P, Cag-P, Al-P and Fe-P were the main forms of inorganic P absorbed by crops. The surplus P from fertilization remained in the 0-20 cm soil layer and increased with the application rate of P. The combined application of NP or NPK increased available P to a lesser extent than the combined application of PK. Fertilizer P had mainly transformed to the Al-P, Fe-P, Ca2-P, and Cas-P forms. By using the P budget (X), changes in total P (Y1) and available P (Y2) in soils under the current cropping system could be predicted by the equations: Y1 = 0.02 + 1.01X and Y2 = 2.08 + 0.15X.