Objective:To investigate the potential of blastocyst development of D3 low quality embryos for blastocyst culturing and freezing. Methods:Two thousand one hundred and eighty embryos of 398 IVF/ICSI patients’ were obs...Objective:To investigate the potential of blastocyst development of D3 low quality embryos for blastocyst culturing and freezing. Methods:Two thousand one hundred and eighty embryos of 398 IVF/ICSI patients’ were observed after D3 embryo transfer.The low-quality embryos were cultured by sequential micro-drops and continuously observed blastocyst formation from D3 to D6. Results:(1) A total of 1,546 low-quality embryos were collected,and 426 blastocysts formed in D5-D6.Total blastocyst formation rate(BFR) was 27.56%,of which embryos with grade 6Ⅲ/Ⅳwere 40.87%(318/778),5Ⅰ/Ⅱ28.85%(30/104),4Ⅰ/Ⅱ8.88%(16/180),4Ⅲ/Ⅳ19.72%(56/284),2-3Ⅰ/Ⅱ3%(6/200).(2) Embryos with more blastomeres had a higher blastocyst formation rate.The lower qualities of embryos resulted in lower blastocyst formation rate. Conclusions:Low-quality embryos still have the potential to develop into blastocysts.Therefore,they should not be discarded on D3.They should be continually cultured to D6 in order to reduce embryo wastage and get good clinical results.展开更多
Objectives:To assess the clinical outcomes of frozen-thawed blastocysts transfer in natural and hormonally controlled cycles.Methods:A retrospective analysis of natural and hormonally controlled cycle for 246 frozen-t...Objectives:To assess the clinical outcomes of frozen-thawed blastocysts transfer in natural and hormonally controlled cycles.Methods:A retrospective analysis of natural and hormonally controlled cycle for 246 frozen-thawed blastocyst transfer cycles,the clinical pregnancy rate,implantation rate,early abortion rate were compared.Results:Of the 192 hormonally controlled cycles,the cancel rate,clinical pregnancy rate per ET,implantation rate and abortion rate were 7.3%(14/192),53.9%(96/178),38.8%(131/338)and 11.5%(11/96)respectively,whereas in 54 natural cycles,these rates were 16.7%(9/54),68.9%(31/45),52.9%(45/85)and 16.1%(5/31)respectively.There was no significant difference between the two groups with regard to the clinical pregnancy and abortion rate per ET,but the cancel rate and implantation rate were higher in natural cycles.However,the pregnancy and implantation rates of patients without PCOS in hormonal control cycles(57.2%,40.9%)were similar with those in natural cycles(P>0.05).Conclusion:These findings suggested that both hormonally controlled and natural cycles had similar pregnancy outcomes in frozen-thawed blastocysts transfer.展开更多
文摘Objective:To investigate the potential of blastocyst development of D3 low quality embryos for blastocyst culturing and freezing. Methods:Two thousand one hundred and eighty embryos of 398 IVF/ICSI patients’ were observed after D3 embryo transfer.The low-quality embryos were cultured by sequential micro-drops and continuously observed blastocyst formation from D3 to D6. Results:(1) A total of 1,546 low-quality embryos were collected,and 426 blastocysts formed in D5-D6.Total blastocyst formation rate(BFR) was 27.56%,of which embryos with grade 6Ⅲ/Ⅳwere 40.87%(318/778),5Ⅰ/Ⅱ28.85%(30/104),4Ⅰ/Ⅱ8.88%(16/180),4Ⅲ/Ⅳ19.72%(56/284),2-3Ⅰ/Ⅱ3%(6/200).(2) Embryos with more blastomeres had a higher blastocyst formation rate.The lower qualities of embryos resulted in lower blastocyst formation rate. Conclusions:Low-quality embryos still have the potential to develop into blastocysts.Therefore,they should not be discarded on D3.They should be continually cultured to D6 in order to reduce embryo wastage and get good clinical results.
文摘Objectives:To assess the clinical outcomes of frozen-thawed blastocysts transfer in natural and hormonally controlled cycles.Methods:A retrospective analysis of natural and hormonally controlled cycle for 246 frozen-thawed blastocyst transfer cycles,the clinical pregnancy rate,implantation rate,early abortion rate were compared.Results:Of the 192 hormonally controlled cycles,the cancel rate,clinical pregnancy rate per ET,implantation rate and abortion rate were 7.3%(14/192),53.9%(96/178),38.8%(131/338)and 11.5%(11/96)respectively,whereas in 54 natural cycles,these rates were 16.7%(9/54),68.9%(31/45),52.9%(45/85)and 16.1%(5/31)respectively.There was no significant difference between the two groups with regard to the clinical pregnancy and abortion rate per ET,but the cancel rate and implantation rate were higher in natural cycles.However,the pregnancy and implantation rates of patients without PCOS in hormonal control cycles(57.2%,40.9%)were similar with those in natural cycles(P>0.05).Conclusion:These findings suggested that both hormonally controlled and natural cycles had similar pregnancy outcomes in frozen-thawed blastocysts transfer.
