The interaction of bleomycinA5 with nucleic acids has been investigated byusing resonance Rayleigh scattering (RRS),molecular absorption and fluorescence spectra.The resultshows that in near pH 2.2 buffer medium and a...The interaction of bleomycinA5 with nucleic acids has been investigated byusing resonance Rayleigh scattering (RRS),molecular absorption and fluorescence spectra.The resultshows that in near pH 2.2 buffer medium and absence of any metal ions,nucleic acids are capable ofbinding with bleomycinA5 (BLMA5) to form complexes which can remarkably enhance the RRS intensityand result in batho-chromic and hyperchromic molecular absorption of nucleic acids and fluorescencequenching of bleomycinA5.The RRS spectral characteristics for the binding products of bleomycinA5with various DNA and RNA are similar,and the maximum RRS peaks are at 301 nm for ctDNA and sDNA,370nm for hsDNA,310 nm for RNAtypeVI and RNAtypelll,respectively.The increments of RRS intensity aregreatly different in which DNA enhances greatly and RNA enhances lightly.In this work,the optimumconditions of the interaction and some influencing factors have been investigated.The reactionmechanism and a binding model for the interaction of BLMA5 with the nucleic acids are discussed.Inaddition,a highly sensitive,simple and rapid new method for the determination of DNA has beendeveloped.The detection limits (3 sigma) are 5.7 ng/mL for ctDNA,7.4 ng/mL for sDNA and 9.2 ng/mLfor hsDNA,respectively.The method can be applied to determination of trace amounts of DNA.展开更多
基金Supported by the National Natural Science Foundation of China (Grant No. 20475045)
文摘The interaction of bleomycinA5 with nucleic acids has been investigated byusing resonance Rayleigh scattering (RRS),molecular absorption and fluorescence spectra.The resultshows that in near pH 2.2 buffer medium and absence of any metal ions,nucleic acids are capable ofbinding with bleomycinA5 (BLMA5) to form complexes which can remarkably enhance the RRS intensityand result in batho-chromic and hyperchromic molecular absorption of nucleic acids and fluorescencequenching of bleomycinA5.The RRS spectral characteristics for the binding products of bleomycinA5with various DNA and RNA are similar,and the maximum RRS peaks are at 301 nm for ctDNA and sDNA,370nm for hsDNA,310 nm for RNAtypeVI and RNAtypelll,respectively.The increments of RRS intensity aregreatly different in which DNA enhances greatly and RNA enhances lightly.In this work,the optimumconditions of the interaction and some influencing factors have been investigated.The reactionmechanism and a binding model for the interaction of BLMA5 with the nucleic acids are discussed.Inaddition,a highly sensitive,simple and rapid new method for the determination of DNA has beendeveloped.The detection limits (3 sigma) are 5.7 ng/mL for ctDNA,7.4 ng/mL for sDNA and 9.2 ng/mLfor hsDNA,respectively.The method can be applied to determination of trace amounts of DNA.
