Objective： To observe changes in RBC count, changes, RBC morphology, and serum potassium during compressed transfusion of WBC-removal whole blood. Methods： Prepared human WBC-removal whole blood and connected transf...Objective： To observe changes in RBC count, changes, RBC morphology, and serum potassium during compressed transfusion of WBC-removal whole blood. Methods： Prepared human WBC-removal whole blood and connected transfusion apparatus with different sizes of intravenous detaining needles （18G, 20G, 22G and 24G）. Observed RBC count and serum potassium concentration under different pressures （100 mmHg, 200 mmHg, and 300 mmHg） as blood flowed out of the pinhead end of the intravenous detaining needle. Samples obtained with the 20G needle were smeared on glass slides, and RBC morphologic changes were observed under an oil immersion lens. Results： RBC count and serum potassium changed slightly under different pressures with different sizes of intravenous detaining needles as blood flowed through the transfusion apparatus. In addition, the observation of blood samples under a common light microscope revealed that coarse- prick, oblong, and spindle cell counts in the visual fields increased gradually as the pressure increased. Additionally, a portion of cells had undergone splintering. Conclusions： While applying 18G, 20G, 22G and 24G intravenous detaining needles for blood transfusion under less than 300 mmHg of pressure, no significant RBC count change was found in blood samples in the short term. However, there were significant RBC morphologic changes. The results could offer more basis to ensure the clinical safetv of oatients undereoing blood transfilsion.展开更多
In order to provide pertinent evidence of ion channel with immune response in the fish, whole cell patch-clamp technique was employed for potassium ion channel study in turbot （Scophthalmus maximus）. Lymphocytes wer...In order to provide pertinent evidence of ion channel with immune response in the fish, whole cell patch-clamp technique was employed for potassium ion channel study in turbot （Scophthalmus maximus）. Lymphocytes were isolated by Percoll density gradient centrifugation from peripheral blood samples, and electrophysiological characters of the channel were analyzed. In the recorded cells, activated voltage of the channels was -42.5±3.7 mV and the average peak current was 313.12±28.2 pA. The channel was identified as voltage dependent, the current was outward and it could be inhibited by 10 mmol/dma TEA or 5 mmol/dm^3 4-AP, a specific potassium channel inhibitor, identifying the existence of potassium channel in peripheral lymphocytes of the turbot.展开更多
The authors conducted a retrospective study over a period of 6 months in a hypertensive population in order to determine the correlation between serum uric acid on glomerular filtration rate (GFR) and estimated serum ...The authors conducted a retrospective study over a period of 6 months in a hypertensive population in order to determine the correlation between serum uric acid on glomerular filtration rate (GFR) and estimated serum potassium with left ventricular hypertrophy (LVH) and obesity. This study enrolled 122 patients including 63 women (51.6%). The mean age was 55.9 ± 10.6 years (range 30 to 74 years). Obesity weight was found in 38 cases (31.1%) of which 20?were men (33.9%) and 18 women (28.6%). Abdominal obesity was found in 104 cases (85.2%). The average serum uric acid in patients with obesity weight was 63.3 ± 18.9 mg/L vs 62.4 ± 14.2 mg/L for no-obese patients (p?= 0.63). The average serum potassium in obese patients was 4.06 ± 0.42 mEq/L vs 4.02 ± 0.46 mEq/L for no-obese (p?= 0.65). The average GFR was 73.4 ± 21.4 ml/L in obese patients vs 66.6 ± 22.6 ml/min in no-obese (p?= 0.03). The LVH was found in 81 cases (66.4%). The LVH was found in 65 (62.5%) obese patients vs?16 (88%) non-obese patients (OR = 4.8, 95% 1.04?-?22?p?= 0.02). Only abdominal?obesity has been correlated with left ventricular hypertrophy after multivariate analysis. Emphasis must be focused on public health actions for effective and appropriate measures against obesity and hypertension, whose prevalence is increasing in our region.展开更多
目的探讨miRNA基因多态性与钠钾饮食干预后血压反应性之间的关系。方法本课题组2004年从中国陕西宝鸡7个村庄的124个家庭中招募514名参与者进行慢性盐负荷试验干预,包括3 d基线期、7 d低盐饮食、7 d高盐饮食和7 d高盐补钾饮食干预。纳...目的探讨miRNA基因多态性与钠钾饮食干预后血压反应性之间的关系。方法本课题组2004年从中国陕西宝鸡7个村庄的124个家庭中招募514名参与者进行慢性盐负荷试验干预,包括3 d基线期、7 d低盐饮食、7 d高盐饮食和7 d高盐补钾饮食干预。纳入19个miRNA-SNP位点进行分析研究。结果在钠钾饮食干预过程中,受试者的血压在低盐期呈下降趋势,高盐期呈现上升趋势,而在高盐补钾后,血压则再次下降。在低盐期,miR-210-3p SNP rs12364149与收缩压反应性、舒张压反应性及平均动脉压反应性显著相关,miR-4638-3p SNP rs6601178与收缩压反应性显著相关,而miR-26b-3p SNP rs115254818与平均动脉压反应性显著相关。在高盐干预后,miR-26b-3p SNP rs115254818与收缩压反应性、舒张压反应性及平均动脉压反应性显著相关;miR-1307-5p SNP rs11191676、rs2292807与收缩压反应性及平均动脉压反应性密切相关;miR-4638-3p SNP rs6601178、miR-210-3p SNP rs12364149以及miR-382-5p SNP rs4906032、rs4143957与收缩压反应性存在显著关联性。此外,在给予补钾干预后miR-26b-3p SNP rs115254818与收缩压反应性、舒张压反应性及平均动脉压反应性关联显著;miR-1307-5p SNP rs11191676、rs2292807以及miR-19a-3p SNP rs4284505与收缩压反应性显著相关。结论miRNA基因多态性与血压钠钾反应性密切相关,提示miRNA基因可能参与血压盐敏感性及钾敏感性的形成。展开更多
基金supported by the Shanxi Science and Technology Development Fund(No.200233)
文摘Objective： To observe changes in RBC count, changes, RBC morphology, and serum potassium during compressed transfusion of WBC-removal whole blood. Methods： Prepared human WBC-removal whole blood and connected transfusion apparatus with different sizes of intravenous detaining needles （18G, 20G, 22G and 24G）. Observed RBC count and serum potassium concentration under different pressures （100 mmHg, 200 mmHg, and 300 mmHg） as blood flowed out of the pinhead end of the intravenous detaining needle. Samples obtained with the 20G needle were smeared on glass slides, and RBC morphologic changes were observed under an oil immersion lens. Results： RBC count and serum potassium changed slightly under different pressures with different sizes of intravenous detaining needles as blood flowed through the transfusion apparatus. In addition, the observation of blood samples under a common light microscope revealed that coarse- prick, oblong, and spindle cell counts in the visual fields increased gradually as the pressure increased. Additionally, a portion of cells had undergone splintering. Conclusions： While applying 18G, 20G, 22G and 24G intravenous detaining needles for blood transfusion under less than 300 mmHg of pressure, no significant RBC count change was found in blood samples in the short term. However, there were significant RBC morphologic changes. The results could offer more basis to ensure the clinical safetv of oatients undereoing blood transfilsion.
基金The National Natural Science Foundation of China under contract Nos 40476060 and 40876089the State Key Basic Research Program under contract No. 2006CB101805
文摘In order to provide pertinent evidence of ion channel with immune response in the fish, whole cell patch-clamp technique was employed for potassium ion channel study in turbot （Scophthalmus maximus）. Lymphocytes were isolated by Percoll density gradient centrifugation from peripheral blood samples, and electrophysiological characters of the channel were analyzed. In the recorded cells, activated voltage of the channels was -42.5±3.7 mV and the average peak current was 313.12±28.2 pA. The channel was identified as voltage dependent, the current was outward and it could be inhibited by 10 mmol/dma TEA or 5 mmol/dm^3 4-AP, a specific potassium channel inhibitor, identifying the existence of potassium channel in peripheral lymphocytes of the turbot.
文摘The authors conducted a retrospective study over a period of 6 months in a hypertensive population in order to determine the correlation between serum uric acid on glomerular filtration rate (GFR) and estimated serum potassium with left ventricular hypertrophy (LVH) and obesity. This study enrolled 122 patients including 63 women (51.6%). The mean age was 55.9 ± 10.6 years (range 30 to 74 years). Obesity weight was found in 38 cases (31.1%) of which 20?were men (33.9%) and 18 women (28.6%). Abdominal obesity was found in 104 cases (85.2%). The average serum uric acid in patients with obesity weight was 63.3 ± 18.9 mg/L vs 62.4 ± 14.2 mg/L for no-obese patients (p?= 0.63). The average serum potassium in obese patients was 4.06 ± 0.42 mEq/L vs 4.02 ± 0.46 mEq/L for no-obese (p?= 0.65). The average GFR was 73.4 ± 21.4 ml/L in obese patients vs 66.6 ± 22.6 ml/min in no-obese (p?= 0.03). The LVH was found in 81 cases (66.4%). The LVH was found in 65 (62.5%) obese patients vs?16 (88%) non-obese patients (OR = 4.8, 95% 1.04?-?22?p?= 0.02). Only abdominal?obesity has been correlated with left ventricular hypertrophy after multivariate analysis. Emphasis must be focused on public health actions for effective and appropriate measures against obesity and hypertension, whose prevalence is increasing in our region.
文摘目的探讨miRNA基因多态性与钠钾饮食干预后血压反应性之间的关系。方法本课题组2004年从中国陕西宝鸡7个村庄的124个家庭中招募514名参与者进行慢性盐负荷试验干预,包括3 d基线期、7 d低盐饮食、7 d高盐饮食和7 d高盐补钾饮食干预。纳入19个miRNA-SNP位点进行分析研究。结果在钠钾饮食干预过程中,受试者的血压在低盐期呈下降趋势,高盐期呈现上升趋势,而在高盐补钾后,血压则再次下降。在低盐期,miR-210-3p SNP rs12364149与收缩压反应性、舒张压反应性及平均动脉压反应性显著相关,miR-4638-3p SNP rs6601178与收缩压反应性显著相关,而miR-26b-3p SNP rs115254818与平均动脉压反应性显著相关。在高盐干预后,miR-26b-3p SNP rs115254818与收缩压反应性、舒张压反应性及平均动脉压反应性显著相关;miR-1307-5p SNP rs11191676、rs2292807与收缩压反应性及平均动脉压反应性密切相关;miR-4638-3p SNP rs6601178、miR-210-3p SNP rs12364149以及miR-382-5p SNP rs4906032、rs4143957与收缩压反应性存在显著关联性。此外,在给予补钾干预后miR-26b-3p SNP rs115254818与收缩压反应性、舒张压反应性及平均动脉压反应性关联显著;miR-1307-5p SNP rs11191676、rs2292807以及miR-19a-3p SNP rs4284505与收缩压反应性显著相关。结论miRNA基因多态性与血压钠钾反应性密切相关,提示miRNA基因可能参与血压盐敏感性及钾敏感性的形成。
基金supported by the Guangdong Provincial Medical Science and Technology Research Fund Project(B2023011)the Guangzhou Science and Technology Plan Project(202102080292)。
