BACKGROUND Gastric cancer is one of the most common malignant tumors worldwide,and surgical resection is one of the main ways to treat gastric cancer.However,the immune status of postoperative patients is crucial for ...BACKGROUND Gastric cancer is one of the most common malignant tumors worldwide,and surgical resection is one of the main ways to treat gastric cancer.However,the immune status of postoperative patients is crucial for prognosis and survival,and immune cells play an important role in this process.Therefore,it is helpful to understand the immune status of postoperative patients by evaluating the levels of peripheral blood immune cells,especially total T cells(CD3+),helper T cells(CD3+CD4+),and suppressor T cells(CD3+CD8+),and its relationship to sur-vival.AIM To analyzed the immune cells in peripheral blood of patients with gastric cancer after surgery,detect the levels of total T cells,helper T cells and suppressor T cells.METHODS A total of 58 patients with gastric cancer who received surgical treatment were included in the retrospective study.Flow cytometry was used to detect the level of peripheral blood immune cells and analyze the correlation between total T cells,helper T cells and inhibitory T cells.To explore the relationship between these immune markers and patient survival.RESULTS The results showed that the levels of total T cells,helper T cells,and suppressor T cells changed in patients after gastric cancer surgery.There was a significant positive correlation between total T cells,helper T cells and suppressor T cells(r=0.35,P<0.01;r=0.56,P<0.01).However,there was a negative correlation between helper T cells and suppressor T cells(r=-0.63,P<0.01).Follow-up showed that the survival rate of patients in the high-level total T cell group was significantly higher than that in the low-level group(28.87±24.98 months vs 18.42±16.21 months).The survival curve shows that the curve of patients in the high-level group is shifted to the upper right,and that of the low-level group is shifted downward.There was no significant difference between the levels of helper T cells and suppressor T cells and patient survival time.CONCLUSION By detecting peripheral blood immune cells with flow cytometry,we can initially evaluate the immune status of patients after gastric cancer surgery and initially explore its relationship with patient survival.展开更多
The cerebral blood flow（CBF） alterations related to sound-induced opening of the blood–brain barrier（BBB） in adult mice are studied using laser speckle contrast imaging（LSCI） and wavelet analysis of vascular ph...The cerebral blood flow（CBF） alterations related to sound-induced opening of the blood–brain barrier（BBB） in adult mice are studied using laser speckle contrast imaging（LSCI） and wavelet analysis of vascular physiology.The results clearly show that the opening of the BBB is accompanied by the changes of venous but not microvessel circulation in the brain. The elevation of the BBB permeability is associated with the decrease of venous CBF and the increase of its complexity. These data suggest that the cerebral veins rather than microvessels are sensitive components of the CBF related to the opening BBB.展开更多
Laser speckle contrast imaging （LSCI） is a powerful tool for blood flow mapping. In this paper, we described a simple algorithm based on histogram analysis of laser speckle contrast image to provide rapid differenti...Laser speckle contrast imaging （LSCI） is a powerful tool for blood flow mapping. In this paper, we described a simple algorithm based on histogram analysis of laser speckle contrast image to provide rapid differentiation between macro- and microcirculations. The algorithm was successfully verified by the study of blood flow in rat cortex under functional activation.展开更多
Blood analysis is a ubiquitous and critical aspect of modern medicine.Analyzing blood samples requires invasive techniques,various testing systems,and samples are limited to relatively small volumes.Photoacoustic imag...Blood analysis is a ubiquitous and critical aspect of modern medicine.Analyzing blood samples requires invasive techniques,various testing systems,and samples are limited to relatively small volumes.Photoacoustic imaging(PAI)is a novel imaging modality that utilizes non-ionizing energy that shows promise as an alternative to current methods.This paper seeks to review current applications of PAI in blood analysis for clinical use.Furthermore,we discuss obstacles to implementation and future directions to overcome these challenges.Firstly,we discuss three applications to cellular analysis of blood:sickle cell,bacteria,and circulating tumor cell detection.We then discuss applications to the analysis of blood plasma,including glucose detection and anticoagulation quantification.As such,we hope this article will serve as inspiration for PAI's potential application in blood analysis and prompt further studies to ultimately implement PAI into clinical practice.展开更多
目的研究高场磁共振三维准连续动脉自旋标记(3D p CASL)序列对头颈部肿瘤非对比剂增强灌注成像的可行性,分析不同标记时间(PLD)对图像质量的影响及肿瘤血流量重复测量的信度。方法前瞻性分析2013年6月至9月在北京协和医院临床发现头颈...目的研究高场磁共振三维准连续动脉自旋标记(3D p CASL)序列对头颈部肿瘤非对比剂增强灌注成像的可行性,分析不同标记时间(PLD)对图像质量的影响及肿瘤血流量重复测量的信度。方法前瞻性分析2013年6月至9月在北京协和医院临床发现头颈部肿瘤但未经过治疗的患者25例,所有患者均行头颈部3D p CASL非对比剂增强灌注扫描。在进行常规T1加权及T2加权成像后,进行3组不同标记后延迟(PLD)时间的ASL序列扫描:ASL1(PLD1=1525 ms)、ASL2(PLD2=2025 ms)和ASL3(PLD3=2525 ms)。测量3组ASL灌注加权图像上肿瘤区域及背景的信号强度(SI),计算肿瘤区域的信噪比(SNR)及对比噪声比(CNR)。由两位观察者分别对3D p CASL的灌注加权图像上螺旋伪影及颈部血管伪影的显示情况进行评分,测量血流量(BF)图像上肿瘤区域的BF值。结果共对17例取得病理结果的患者进行3D p CASL的图像质量评价及肿瘤的BF值测量。ASL1、ASL2及ASL3的SNR及CNR均依次降低,其中ASL1与ASL3的SNR(P=0.011)及CNR(P=0.009)差异有统计学意义。3组ASL螺旋伪影评分差异无统计学意义(P=0.932)。ASL1、ASL2及ASL3灌注加权图像颈部血管伪影的定性评分均值依次减低,其中ASL1与ASL3的差异有统计学意义(P=0.000)。3组BF值的观察者间及观察者内重复测量的信度水平均非常好(ICC>0.9)。尽管ASL1、ASL2及ASL3序列的BF均值依次升高,但差异无统计学意义(P=0.977)。结论采用3D p CASL高场磁共振进行头颈部肿瘤非对比剂增强灌注成像可以获得比较满意的图像质量,并且肿瘤BF值具有较好的重复测量信度。在3组3D p CASL序列中,PLD=1525 ms及PLD=2025 ms序列的图像质量优于PLD=2525 ms序列,且BF值无明显差异,因此更适合头颈部肿瘤3D p CASL灌注成像。展开更多
文摘BACKGROUND Gastric cancer is one of the most common malignant tumors worldwide,and surgical resection is one of the main ways to treat gastric cancer.However,the immune status of postoperative patients is crucial for prognosis and survival,and immune cells play an important role in this process.Therefore,it is helpful to understand the immune status of postoperative patients by evaluating the levels of peripheral blood immune cells,especially total T cells(CD3+),helper T cells(CD3+CD4+),and suppressor T cells(CD3+CD8+),and its relationship to sur-vival.AIM To analyzed the immune cells in peripheral blood of patients with gastric cancer after surgery,detect the levels of total T cells,helper T cells and suppressor T cells.METHODS A total of 58 patients with gastric cancer who received surgical treatment were included in the retrospective study.Flow cytometry was used to detect the level of peripheral blood immune cells and analyze the correlation between total T cells,helper T cells and inhibitory T cells.To explore the relationship between these immune markers and patient survival.RESULTS The results showed that the levels of total T cells,helper T cells,and suppressor T cells changed in patients after gastric cancer surgery.There was a significant positive correlation between total T cells,helper T cells and suppressor T cells(r=0.35,P<0.01;r=0.56,P<0.01).However,there was a negative correlation between helper T cells and suppressor T cells(r=-0.63,P<0.01).Follow-up showed that the survival rate of patients in the high-level total T cell group was significantly higher than that in the low-level group(28.87±24.98 months vs 18.42±16.21 months).The survival curve shows that the curve of patients in the high-level group is shifted to the upper right,and that of the low-level group is shifted downward.There was no significant difference between the levels of helper T cells and suppressor T cells and patient survival time.CONCLUSION By detecting peripheral blood immune cells with flow cytometry,we can initially evaluate the immune status of patients after gastric cancer surgery and initially explore its relationship with patient survival.
基金supported by the Grant of Russian Science Foundation No 17-15-01263supported by the Open Research Fund of Key Laboratory for Biomedical Photonics,HUST,Ministry of Education,China
文摘The cerebral blood flow（CBF） alterations related to sound-induced opening of the blood–brain barrier（BBB） in adult mice are studied using laser speckle contrast imaging（LSCI） and wavelet analysis of vascular physiology.The results clearly show that the opening of the BBB is accompanied by the changes of venous but not microvessel circulation in the brain. The elevation of the BBB permeability is associated with the decrease of venous CBF and the increase of its complexity. These data suggest that the cerebral veins rather than microvessels are sensitive components of the CBF related to the opening BBB.
文摘Laser speckle contrast imaging （LSCI） is a powerful tool for blood flow mapping. In this paper, we described a simple algorithm based on histogram analysis of laser speckle contrast image to provide rapid differentiation between macro- and microcirculations. The algorithm was successfully verified by the study of blood flow in rat cortex under functional activation.
基金This research was funded by The United States National Institutes of Health(NIH)grants R38HL143612,R21EB027981,R21 EB027304,RF1 NS115581(BRAIN Initiative),R01 NS111039,R01 EB028143The United States National Science Foundation(NSF)CAREER award 2144788Chan Zuckerberg Initiative Grant(2020-226178).
文摘Blood analysis is a ubiquitous and critical aspect of modern medicine.Analyzing blood samples requires invasive techniques,various testing systems,and samples are limited to relatively small volumes.Photoacoustic imaging(PAI)is a novel imaging modality that utilizes non-ionizing energy that shows promise as an alternative to current methods.This paper seeks to review current applications of PAI in blood analysis for clinical use.Furthermore,we discuss obstacles to implementation and future directions to overcome these challenges.Firstly,we discuss three applications to cellular analysis of blood:sickle cell,bacteria,and circulating tumor cell detection.We then discuss applications to the analysis of blood plasma,including glucose detection and anticoagulation quantification.As such,we hope this article will serve as inspiration for PAI's potential application in blood analysis and prompt further studies to ultimately implement PAI into clinical practice.
文摘目的研究高场磁共振三维准连续动脉自旋标记(3D p CASL)序列对头颈部肿瘤非对比剂增强灌注成像的可行性,分析不同标记时间(PLD)对图像质量的影响及肿瘤血流量重复测量的信度。方法前瞻性分析2013年6月至9月在北京协和医院临床发现头颈部肿瘤但未经过治疗的患者25例,所有患者均行头颈部3D p CASL非对比剂增强灌注扫描。在进行常规T1加权及T2加权成像后,进行3组不同标记后延迟(PLD)时间的ASL序列扫描:ASL1(PLD1=1525 ms)、ASL2(PLD2=2025 ms)和ASL3(PLD3=2525 ms)。测量3组ASL灌注加权图像上肿瘤区域及背景的信号强度(SI),计算肿瘤区域的信噪比(SNR)及对比噪声比(CNR)。由两位观察者分别对3D p CASL的灌注加权图像上螺旋伪影及颈部血管伪影的显示情况进行评分,测量血流量(BF)图像上肿瘤区域的BF值。结果共对17例取得病理结果的患者进行3D p CASL的图像质量评价及肿瘤的BF值测量。ASL1、ASL2及ASL3的SNR及CNR均依次降低,其中ASL1与ASL3的SNR(P=0.011)及CNR(P=0.009)差异有统计学意义。3组ASL螺旋伪影评分差异无统计学意义(P=0.932)。ASL1、ASL2及ASL3灌注加权图像颈部血管伪影的定性评分均值依次减低,其中ASL1与ASL3的差异有统计学意义(P=0.000)。3组BF值的观察者间及观察者内重复测量的信度水平均非常好(ICC>0.9)。尽管ASL1、ASL2及ASL3序列的BF均值依次升高,但差异无统计学意义(P=0.977)。结论采用3D p CASL高场磁共振进行头颈部肿瘤非对比剂增强灌注成像可以获得比较满意的图像质量,并且肿瘤BF值具有较好的重复测量信度。在3组3D p CASL序列中,PLD=1525 ms及PLD=2025 ms序列的图像质量优于PLD=2525 ms序列,且BF值无明显差异,因此更适合头颈部肿瘤3D p CASL灌注成像。
