利用生物素标记法和 Western blotting技术检测血小板膜糖蛋白

为了建立一种简便的血小板膜糖蛋白检测方法,用生物素标记血小板膜糖蛋白,然后用westernblotting法进行检测。结果显示生物素可标记十几种血小板膜糖蛋白。用以标记的生物素浓度以5mmol/L较为适宜。在Western blotting图谱上,血小板无力症患者的膜糖蛋白Ⅱb、Ⅲa与正常对照相比明显减少。结果提示该方法可用于血小板膜糖蛋白减少性疾病的检测。

Quantitative effect of kerogen type on the hydrocarbon generation potential of Paleogene lacustrine source rocks,Liaohe Western Depression,China

Kerogen types exert a decisive effect on the onset and capacity of hydrocarbon generation of source rocks.Lacustrine source rocks in the Liaohe Western Depression are characterized by thick deposition,high total organic carbon(TOC)content,various kerogen types,and a wide range of thermal maturity.Consequently,their hydrocarbon generation potential and resource estimation can be misinterpreted.In this study,geochemical tests,numerical analysis,hydrocarbon generation kinetics,and basin modeling were integrated to investigate the differential effects of kerogen types on the hydrocarbon generation potential of lacustrine source rocks.Optimized hydrocarbon generation and expulsion(HGE)models of different kerogen types were established quantitatively upon abundant Rock-Eval/TOC/vitrinite reflectance(R_(o))datasets.Three sets of good-excellent source rocks deposited in the fourth(Es4),third(Es3),and first(Es1)members of Paleogene Shahejie Formation,are predominantly types I-II_(1),II_(1)-II_(2),and II-III,respectively.The activation energy of types I-II_(2)kerogen is concentrated(180-230 kcal/mol),whereas that of type III kerogen is widely distributed(150-280 kcal/mol).The original hydrocarbon generation potentials of types I,II_(1),II_(2),and III kerogens are 790,510,270,and 85 mg/g TOC,respectively.The Ro values of the hydrocarbon generation threshold for type I-III source rocks gradually increase from 0.42%to 0.74%,and Ro values of the hydrocarbon expulsion threshold increase from 0.49%to 0.87%.Types I and II_(1)source rocks are characterized by earlier hydrocarbon generation,more rapid hydrocarbon expulsion,and narrower hydrocarbon generation windows than types II_(2)and III source rocks.The kerogen types also affect the HGE history and resource potential.Three types(conventional,tight,and shale oil/gas)and three levels(realistic,expected,and prospective)of hydrocarbon resources of different members in the Liaohe Western Depression are evaluated.Findings suggest that the Es3 member has considerable conventional and unconventional hydrocarbon resources.This study can quantitatively characterize the hydrocarbon generation potential of source rocks with different kerogen types,and facilitate a quick and accurate assessment of hydrocarbon resources,providing strategies for future oil and gas exploration.

Quality of life and survival analyses of breast cancer cases treated with integrated traditional Chinese and Western medicine

BACKGROUND Breast cancer(BC)is the second leading cause of tumor-related mortality after lung cancer.Chemotherapy resistance remains a major challenge to progress in BC treatment,warranting further exploration of feasible and effective alternative therapies.AIM To analyzed the quality of life(QoL)and survival of patients with BC treated with integrated traditional Chinese and Western medicine(TCM-WM).METHODS This study included 226 patients with BC admitted to the First Affiliated Hospital of Guangxi University of Traditional Chinese Medicine between February 2018 and February 2023,including 100 who received conventional Western medicine treatment(control group)and 126 who received TCM-WM treatment(research group).The total effective rate,side effects(alopecia,nausea and vomiting,hepatorenal toxicity,and myelosuppression),QoL assessed using the European Organization for Research and Treatment of Cancer Core Quality of Life Questionnaire(EORTC QLQ-C30),1-year overall survival(OS),recurrence and metastasis rates,and serum inflammatory factors[interleukin(IL)-6,IL-10,and tumor necrosis factor alpha]were comparatively analyzed.RESULTS The research group showed statistically better overall efficacy,EORTC QoL-C30 scores,and 1-year OS than the control group,with markedly lower side effects and 1-year recurrence and metastasis rates.Moreover,the posttreatment levels of serum inflammatory in the research group were significantly lower than the baseline and those in the control group.CONCLUSION Overall,TCM-WM demonstrated significantly improved therapeutic efficacy while ensuring drug safety in BC,which not only improved patients’QoL and prolonged survival,but also significantly inhibited the inflammatory response.

Application of multidisciplinary team-based integrated traditional Chinese medicine and Western medicine in rotator cuff injury patients undergoing arthroscopic surgery

BACKGROUND Arthroscopic rotator cuff repair is a common surgical treatment for rotator cuff injuries(RCIs).Although this procedure has certain clinical advantages,it requires rehabilitation management interventions to ensure therapeutic efficacy.AIM To investigate the effect of integrated traditional Chinese medicine and Western medicine(TCM-WM)under the multidisciplinary team(MDT)model on the postoperative recovery of patients undergoing arthroscopic surgery for RCIs.METHODS This study enrolled 100 patients who underwent arthroscopic rotator cuff repair for RCIs at the Seventh People’s Hospital of Shanghai University of Traditional Chinese Medicine between June 2021 and May 2024.They were divided into a control group(n=48)that received routine rehabilitation treatment and an experimental group(n=52)that received TCM-WM under the MDT model(e.g.,acupuncture,TCM traumatology and orthopedics,and rehabilitation).The results of the Constant–Murley Shoulder Score(CMS),Visual Analogue Scale(VAS),Shoulder Pain and Disability Index(SPADI),muscular strength evaluation,and shoulder range of motion(ROM)assessments were analyzed.RESULTS After treatment,the experimental group showed significantly higher CMS scores in terms of pain,functional activity,shoulder joint mobility,and muscular strength than the baseline and those of the control group.The experimental group also exhibited significantly lower VAS and SPADI scores than the baseline and those of the control group.In addition,the experimental group showed significantly enhanced muscular strength(forward flexor and external and internal rotator muscles)and shoulder ROM(forward flexion,abduction,and lateral abduction)after treatment compared with the control group.CONCLUSION TCM-WM under the MDT model improved shoulder joint function,relieved postoperative pain,promoted postoperative functional recovery,and facilitated the recovery of muscular strength and shoulder ROM in patients with RCIs who underwent arthroscopic rotator cuff repair.

Research Progress on Combined Chinese and Western Medicine Treatment of Mycoplasma pneumoniae Pneumonia in Children

With the continuous development of medical technology,combined treatment of Chinese and Western medicine has gradually become a research hotspot.As a common disease in pediatrics,the treatment of Mycoplasma pneumoniae pneumonia(MPP)in children is also being explored and improved.This article summarizes the research progress of combined Chinese and Western medicine treatment of MPP in children in recent years,aiming to provide a useful reference for the combined treatment of MPP in children.The article firstly introduces the etiology and pathogenesis of MPP in children,thereafter briefly introduces the Western anti-infective treatment and traditional Chinese medicine(TCM)diagnosis and treatment of MPP in children,and lastly introduces the methods of combined treatment of TCM and Western medicine in detail.The article points out that the combination of Chinese and Western medicine can give full play to the overall regulation of Chinese medicine and the precise treatment advantages of Western medicine,improve the therapeutic effect,reduce the use of antibiotics,and lower the recurrence rate of the disease,which is worthy of further research and promotion.

A Case Report on Treating Progressive Supranuclear Palsy with a Combination of Chinese and Western Medicine

A case of progressive supranuclear palsy in a 66-year-old woman is presented.The patient complained of“progressive stiffness of both lower limbs for five years,accompanied by choking and coughing for more than 1 year,”and was diagnosed with paralysis in traditional Chinese medicine(TCM),which was characterized by a deficiency of liver and kidney,and phlegm and blood stasis blocking the collaterals.Western medicine diagnosed it as progressive supranuclear palsy.The patient was diagnosed with“Parkinson’s disease”several times in the past and was given“polybasic hydrazide”and“amantadine hydrochloride tablets,”etc.The patient did not have significant symptomatic relief,so she sought further treatment with a combination of traditional Chinese and Western medicine.Based on the concept of deficiency and excess,this disease is categorized as a paralytic disease in Chinese medicine,and the onset of the disease is closely related to the deficiency of the body after a long period of illness,with wind,phlegm,blood stasis,and deficiency as the main pathogenetic mechanism.The treatment is based on the combination of disease and evidence,with traditional Chinese medicine and acupuncture as the main treatments,supplemented by intermediate-frequency pulsed electrical stimulation,transcranial repetitive magnetic stimulation,medicated canisters,wax therapy,traditional Chinese medicine guasha,and acupoint injections.Western medicine is based on symptomatic treatment,and the patient’s symptoms were relieved significantly after the combination of Chinese and Western medicine.

疯牛病和羊痒病Western blotting检测方法的建立

以朊蛋白单抗AH6和碱性磷酸酶标记的马抗鼠酶标二抗建立了疯牛病和羊痒病的Western blotting检测方法。对Western blotting各种反应条件进行摸索,并确定了最佳工作条件,结果表明:匀浆缓冲液为RIPA时的最佳反应条件包括浓缩胶电泳电压为恒压90V,分离胶电泳电压为恒压160V,转印的最佳电压和时间为恒压100V1.5h;封闭液为3%BSA时,封闭15min,封闭效果最好;AH6的最佳稀释度为1∶4000,4℃下孵育过夜,马抗鼠二抗的最佳稀释度1∶1000,室温下孵育30min。采用已确立的反应条件对样品进行检测并与Prionics-Check WEST-ERN进口试剂盒的检测结果比较,发现其敏感性为100%,特异性为99.4%,与进口试剂盒(100%,100%)无显著差异,这为国产试剂盒研制提供了条件。

拟南芥DBB1a(double B-box 1a)蛋白的表达、纯化及Western blotting检测

PCR扩增拟南芥(Arabidopsis thaliana)DBB1a cDNA的保守区段(GenBank登录号:AT2G21320),转化到冷诱导表达载体pCold TF上,构建pCold-DBB1a重组质粒,转化大肠杆菌DH5a。15℃下IPTG诱导表达融合蛋白,并通过SDS-PAGE检测。证实目的蛋白以可溶形式在约20 kD处高效表达,与预期蛋白大小相吻合。表达蛋白经Ni琼脂糖凝胶亲和层析纯化,SDS-PAGE及Western blotting检测证实纯化后获得高纯度融合蛋白,这为进一步研究DBBl a功能奠定了基础。

A Physics-informed Deep-learning Intensity Prediction Scheme for Tropical Cyclones over the Western North Pacific

Accurate prediction of tropical cyclone(TC)intensity is challenging due to the complex physical processes involved.Here,we introduce a new TC intensity prediction scheme for the western North Pacific(WNP)based on a time-dependent theory of TC intensification,termed the energetically based dynamical system(EBDS)model,together with the use of a long short-term memory(LSTM)neural network.In time-dependent theory,TC intensity change is controlled by both the internal dynamics of the TC system and various environmental factors,expressed as environmental dynamical efficiency.The LSTM neural network is used to predict the environmental dynamical efficiency in the EBDS model trained using besttrack TC data and global reanalysis data during 1982–2017.The transfer learning and ensemble methods are used to retrain the scheme using the environmental factors predicted by the Global Forecast System(GFS)of the National Centers for Environmental Prediction during 2017–21.The predicted environmental dynamical efficiency is finally iterated into the EBDS equations to predict TC intensity.The new scheme is evaluated for TC intensity prediction using both reanalysis data and the GFS prediction data.The intensity prediction by the new scheme shows better skill than the official prediction from the China Meteorological Administration(CMA)and those by other state-of-art statistical and dynamical forecast systems,except for the 72-h forecast.Particularly at the longer lead times of 96 h and 120 h,the new scheme has smaller forecast errors,with a more than 30%improvement over the official forecasts.

Natural gas exploration potential and favorable targets of Permian Fengcheng Formation in the western Central Depression of Junggar Basin,NW China

Based on the organic geochemical data and the molecular and stable carbon isotopic compositions of natural gas of the Lower Permian Fengcheng Formation in the western Central Depression of Junggar Basin,combined with sedimentary environment analysis and hydrocarbon-generating simulation,the gas-generating potential of the Fengcheng source rock is evaluated,the distribution of large-scale effective source kitchen is described,the genetic types of natural gas are clarified,and four types of favorable exploration targets are selected.The results show that:(1)The Fengcheng Formation is a set of oil-prone source rocks,and the retained liquid hydrocarbon is conducive to late cracking into gas,with characteristics of high gas-generating potential and late accumulation;(2)The maximum thickness of Fengcheng source rock reaches 900 m.The source rock has entered the main gas-generating stage in Penyijingxi and Shawan sags,and the area with gas-generating intensity greater than 20×10^(8) m^(3)/km^(2) is approximately 6500 km^(2).(3)Around the western Central Depression,highly mature oil-type gas with light carbon isotope composition was identified to be derived from the Fengcheng source rocks mainly,while the rest was coal-derived gas from the Carboniferous source rock;(4)Four types of favorable exploration targets with exploration potential were developed in the western Central Depression which are structural traps neighboring to the source,stratigraphic traps neighboring to the source,shale-gas type within the source,and structural traps within the source.Great attention should be paid to these targets.

医学检验专业开设Western Blotting综合性实验初探

分子生物学是当前生命科学发展的主流，其理论与技术已广泛应用于各个领域。Western Blottlng作为分子生物学的常规技术，在医学领域具有不可忽视的地位。因此，在医学检验专业开设Western Blotting实验课程，已成为一项非常必要的实验教学内容。其一，Western Blotting实验是分子生物学与免疫学的有效结合体，有助于学生对前沿的、跨学科知识加深了解；

Geomorphic signatures and active tectonics in western Saurashtra,Gujarat,India

Active tectonics in an area includes ongoing or recent geologic events.This paper investigates the tectonic influence on the subsidence,uplift and tilt of western Saurashtra through morphotectonic analysis of ten watersheds along with characteristics of relief and drainage orientation.Watersheds 7-9 to the north(N)are tectonically active,which can be linked with the North Kathiawar Fault System(NKFS)and followed by watersheds 6,10,1,4 and 5.Stream-length gradient index and sinuosity index indicate the effect of tectonic events along the master streams in watersheds 6-9.Higher R^(2)values of the linear curve fit for watershed 7 indicate its master stream is much more tectonically active than the others.The R^(2)curve fitting model and earthquake magnitude/depth analysis confirm the region to be active.The reactivation of the NKFS most likely led to the vertical movement of western Saurashtra.

Geophysical and Crustal Movement Characteristic of Western Sichuan

Analysis and research of large complex phenomena before and after the devastating earthquake to reduce the threat of natural disasters to human survival environment is of great significance.This paper analyzes the Wenchuan earthquake zone characteristics of gravity anomaly distributions,and then use edge detection and Euler deconvolution method to inverse Longmenshan gravity anomaly before earthquake.Fault distribution features and the general depth about the fault top of Longmenshan and its adjacent area before earthquake has been obtained.Morphology difference and possible earthquake formation have been analyzed through the Euler deconvolution result of gravity anomaly profile before and after the earthquake.

Food Hygiene and Safety Practices amongst Food Vendors in the Western Area, Sierra Leone

Food safety and hygiene practices require a multisectoral approach including food, water, sanitation, waste management, transport, education, trade, policies and programs that enable safe food preparation, storage and service. Unsafe food can cause illness keeping people from achieving their full potential and death. This was a descriptive study that uses a mixed method approach to derive insights into the characteristics of food vendors related to demography, knowledge, practices, infrastructure, compliance and recommendation for a policymaking framework. Using the Lemeshows’ sample size formula, 473 vendors from formal (restaurants) and informal (cookri-baffa/table top) sites were interviewed and observed. We found from discussions that respondents had a good understanding on how to keep food safe. However, observed practices were poor 93% handled food with their bare hands, 83% did not cover their hair, and 76% did not wear an apron whilst handling, preparing or serving food, 61% did not keep their finger nails clean or short and 57% did not wash their hand before preparing or serving food. Over half (51%) had access to a toilet but 32% reported their use required payment and emphasized their poor condition/inadequate management. Nearly half (47%) of the vending sites did not have a handwashing facility, with soap and water available. Only 7% reported having any authority oversight of food safety. Food safety and hygiene practices in most cookri shops and restaurants was ‘poor’ with very limited surveillance system in place by competent authorities for compliance of food operators. Hand washing, clean surroundings, and covered food were the most common and emphasized practices to mitigate the risks associated with unsafe food.

Climate warming is significantly influenced by rising summer maximum temperatures: insights from tree-ring evidence of the Western Tianshan Mountains, China

As one of the regions most affected by global cli-mate warming,the Tianshan mountains has experienced sev-eral ecological crises,including retreating glaciers and water deficits.Climate warming in these mountains is considered mainly to be caused by increases in minimum temperatures and winter temperatures,while the influence of maximum temperatures is unclear.In this study,a 300-year tree-ring chronology developed from the Western Tianshan Moun-tains was used to reconstruct the summer(June-August)maximum temperature(T_(max6-8))variations from 1718 to 2017.The reconstruction explained 53.1% of the variance in the observed T_(max6-8).Over the past 300 years,the T_(max6-8)reconstruction showed clear interannual and decadal vari-abilities.There was a significant warming trend(0.18°C/decade)after the 1950s,which was close to the increasing rates of the minimum and mean temperatures.The increase in maximum temperature was also present over the whole Tianshan mountains and its impact on climate warming has increased.The T_(max6-8) variations in the Western Tianshan mountains were influenced by frequent volcanic eruptions combined with the influence of solar activity and the sum-mer North Atlantic Oscillation.This study reveals that cli-mate warming is significantly influenced by the increase in maximum temperatures and clarifies possible driving mech-anisms of temperature variations in the Western Tianshan mountains which should aid climate predictions.

Climate warming is significantly influenced by rising summer maximum temperatures:insights from tree-ring evidence of the Western Tianshan Mountains,China

As one of the regions most affected by global climate warming,the Tianshan mountains has experienced several ecological crises,including retreating glaciers and water deficits.Climate warming in these mountains is considered mainly to be caused by increases in minimum temperatures and winter temperatures,while the influence of maximum temperatures is unclear.In this study,a 300-year tree-ring chronology developed from the Western Tianshan Mountains was used to reconstruct the summer(June-August)maximum temperature(Tmax6-8) variations from 1718 to2017.The reconstruction explained 53.1% of the variance in the observed Tmax6-8.Over the past 300 years,the Tmax6-8reconstruction showed clear interannual and decadal variabilities.There was a significant warming trend(0.18 ℃/decade) after the 1950s,which was close to the increasing rates of the minimum and mean temperatures.The increase in maximum temperature was also present over the whole Tianshan mountains and its impact on climate warming has increased.The Tmax6-8variations in the Western Tianshan mountains were influenced by frequent volcanic eruptions combined with the influence of solar activity and the summer North Atlantic Oscillation.This study reveals that climate warming is significantly influenced by the increase in maximum temperatures and clarifies possible driving mechanisms of temperature variations in the Western Tianshan mountains which should aid climate predictions.

Impacts of hydropower-induced flow alterations on composition and diversity of riparian vegetation in the Western Himalayas: A case study in Uttarakhand, India

The increasing demand for water and energy resources has led to widespread dam construction,particularly in ecologically sensitive regions like the Himalayan Range.This study focuses on the Uttarakhand state in the Western Himalayas,where hydroelectric projects(HEPs)have significantly altered river flow regimes.The research investigates the impact of flow alterations on the composition and structure of riparian vegetation in the Garhwal Himalayas,specifically analysing four rivers regulated by hydroelectric projects.Utilizing the paired-reach comparison method,control(undisturbed),diverted(downstream of barrage/dam),and altered flow conditions(downstream of water outlet)were examined.The research reveals diverse and unique riparian ecosystems,with 89 genera and 113 taxa identified,showcasing the dominance of families like Asteraceae and Lamiaceae.The study unveils the structural importance of key species such as Berberis asiatica and Artemisia nilagirica.The density,diversity,and richness of shrub and herb species vary significantly across flow conditions.Notably,altered flow conditions demonstrate resilience in vegetation structure,while diverted conditions exhibit decreased species richness and density.The study emphasizes the importance of nuanced environmental flow management for mitigating adverse effects on riparian biodiversity in the fragile Himalayan region.These findings contribute to the global discourse on dam impacts and riparian ecology,shedding light on the complexities of this dynamic relationship in a vulnerable ecosystem.

Impact of typical environments in China’s western mountainous areas on the durability of railway concrete:a review

Purpose–This study aims to analyze the impact mechanism of typical environments in China’s western mountainous areas on the durability of railway concrete and propose measures to improve durability.Design/methodology/approach–With the continuous promotion of infrastructure construction,the focus of China’s railway construction has gradually shifted to the western region.The four typical environments of large temperature differences,strong winds and dryness,high cold and low air pressure unique to the western mountainous areas of China have adverse effects on the durability of typical railway structure concrete(bridges,ballastless tracks and tunnels).This study identified the characteristics of four typical environments in the western mountainous areas of China through on-site research.The impact mechanism of the four typical environments on the durability of concrete in different structural parts of railways has been explored through theoretical analysis and experimental research;Finally,a strategy for improving the durability of railway concrete suitable for the western mountainous areas of China was proposed.Findings–The daily temperature difference in the western mountainous areas of China is more than twice that of the plain region,which will lead to significant temperature deformation and stress in the multi-layered structure of railway ballastless tracks.It will result in cracking.The wind speed in the western plateau region is about 2.5 to 3 times that of the plain region,and the average annual rainfall is only 1/5 of that in the plain region.The drying effect on the surface of casting concrete will significantly accelerate its cracking process,leading to serious durability problems.The environmental temperature in the western mountainous areas of China is generally low,and there are more freeze-thaw cycles,which will increase the risk of freeze-thaw damage to railway concrete.The environmental air pressure in the western plateau region is only 60%of that in the plain region.The moisture inside the concrete is more likely to diffuse into the surrounding environment under the pressure difference,resulting in greater water loss and shrinkage deformation of the concrete in the plateau region.The above four issues will collectively lead to the rapid deterioration of concrete durability in the western plateau region.The corresponding durability improvement suggestions from theoretical research,new technology development and standard system was proposed in this paper.Originality/value–The research can provide the mechanism of durability degradation of railway concrete in the western mountainous areas of China and corresponding improvement strategies.

Track-Pattern-Based Characteristics of Extratropical Transitioning Tropical Cyclones in the Western North Pacific

Based on the Regional Specialized Meteorological Center(RSMC)Tokyo-Typhoon Center best-track data and the NCEP-NCAR reanalysis dataset,extratropical transitioning(ET)tropical cyclones(ETCs)over the western North Pacific(WNP)during 1951–2021 are classified into six clusters using the fuzzy c-means clustering method(FCM)according to their track patterns.The characteristics of the six hard-clustered ETCs with the highest membership coefficient are shown.Most tropical cyclones(TCs)that were assigned to clusters C2,C5,and C6 made landfall over eastern Asian countries,which severely threatened these regions.Among landfalling TCs,93.2%completed their ET after landfall,whereas 39.8%of ETCs completed their transition within one day.The frequency of ETCs over the WNP has decreased in the past four decades,wherein cluster C5 demonstrated a significant decrease on both interannual and interdecadal timescales with the expansion and intensification of the western Pacific subtropical high(WPSH).This large-scale circulation pattern is favorable for C2 and causes it to become the dominant track pattern,owning to it containing the largest number of intensifying ETCs among the six clusters,a number that has increased insignificantly over the past four decades.The surface roughness variation and three-dimensional background circulation led to C5 containing the maximum number of landfalling TCs and a minimum number of intensifying ETCs.Our results will facilitate a better understanding of the spatiotemporal distributions of ET events and associated environment background fields,which will benefit the effective monitoring of these events over the WNP.

Introduction of Western Medicines into China and Improvement on Decoctions during Modern China

During the late Qing dynasty(1840 A.D.-1912 A.D.),a large quantity of Western medicines entered China,which continuously impacted the traditional Chinese medicine(TCM)market and revealed the shortcomings of Chinese medicines.Some personages in the TCM community followed the trend of learning from the West,and attempted to reform TCM,with the improvement on decoction becoming an important aspect of this effort.Through debates and trials,the improvement on decoction underwent three stages of conceptual evolution:“taking Chinese medicines as the foundation and referring to the dosage forms of Western medicines”,“introducing Western techniques to serve the preparation of decoctions”and“integrating the theories of TCM and Western medicine to improve decoctions”.The study highlights the effective complementarity between modern TCM and Western medicine in the field of pharmacy,and provides valuable experience and support for the reevaluation of the value of TCM in contemporary society.