The analytical method developed is based on HPLC-MS/MS to simultanous determine Progesterone,Boldenone and Trenbolone in Chicken,Beef and Pork meats.The sample is freeze drying reduced in powder and a Methanol-Acetate...The analytical method developed is based on HPLC-MS/MS to simultanous determine Progesterone,Boldenone and Trenbolone in Chicken,Beef and Pork meats.The sample is freeze drying reduced in powder and a Methanol-Acetate Buffer (pH=5.2) solution is added in order to perform the extraction and sonicated with a solution prior to deconjugation using β-glucosidase.The sample is purified using C18 and SiOH solid phase extraction (SPE) cartridge prior to analyze on reversed phase with a elution gradient performed on Agilent C18 coupled with a HPLC-MS/MS.The detection Limit is respectively 0.11,0.17 and 0.02 μg/kg for Trenbolone,Boldenone and Progesterone with a ratio Signal/Noise>3 in the 3 different kind of meat.The quantification was based on the peak area and overall recoveries of synthetic growth hormones were 62%-99%.展开更多
文摘The analytical method developed is based on HPLC-MS/MS to simultanous determine Progesterone,Boldenone and Trenbolone in Chicken,Beef and Pork meats.The sample is freeze drying reduced in powder and a Methanol-Acetate Buffer (pH=5.2) solution is added in order to perform the extraction and sonicated with a solution prior to deconjugation using β-glucosidase.The sample is purified using C18 and SiOH solid phase extraction (SPE) cartridge prior to analyze on reversed phase with a elution gradient performed on Agilent C18 coupled with a HPLC-MS/MS.The detection Limit is respectively 0.11,0.17 and 0.02 μg/kg for Trenbolone,Boldenone and Progesterone with a ratio Signal/Noise>3 in the 3 different kind of meat.The quantification was based on the peak area and overall recoveries of synthetic growth hormones were 62%-99%.
